检索结果-南通市图书馆

Synthetic and Systems Biotechnology 2024年第2期9卷 330-339页

作者： Kristin V.Presnell Omar Melhem Sarah M.coleman Nicholas j.Morse Hal S.Alper McKetta Department of Chemical Engineering The University of Texas at Austin200E Dean Keeton St.Stop C0400AustinTX78712USA Institute for Cellular and Molecular Biology The University of Texas at Austin2500 Speedway AvenueAustinTX78712USA

Metabolic engineering and synthetic biology endeavors benefit from promoters that perform consistently(or robustly)with respect to cellular growth phase(exponential and stationary)and fermentation scale(microtiter plates,tubes,flasks,and bioreactors).However,nearly all endogenous promoters(especially in Saccharomyces cerevisiae)do not perform in this *** this work,a hybrid promoter engineering strategy is leveraged to create novel synthetic promoters with robustness across these *** a multi-dimensional rNA-seq dataset,promoters with specific phase dependencies were *** enriched with functional transcription factors were identified using MEME *** motif-containing fragments could impart activity dependence in the opposing ***,we obtain two new promoters with high and consistent expression across both phases by increasing the exponential phase activity of the starting stationary-phase scaffold by 38 and 23-fold ***,we show that these promoters function consistently across various laboratory growth scales over time in a microtiter plate and in ***,this work presents and validates a new strategy for engineering promoters in *** with high levels of expression that are robust to cellular growth phase and the scale of the culture.

关键词： endogenous synthetic stationary

Individual consistency in spatiotemporal characteristics of migratory Whimbrels in the East Asian-Australasian Flyway

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Avian research 2024年第3期15卷 308-315页

作者： Siwei An Fenliang Kuang Wei Wu Chris j.Hassell jonathan T.coleman Zijing Gao Xuena Sun Yue Yuan Grace Maglio Kar-Sin K.Leung Xuesong Feng Zhijun Ma Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Coastal Ecosystems Research Station of the Yangtze River EstuaryInstitute of Biodiversity ScienceSchool of Life SciencesFudan UniversityShanghai200433China School of Chemistry and Environment Yunnan Minzu UniversityKunming650504China Shanghai Chongming Dongtan Nature reserve Administration Center ChongmingShanghai202183China Global Flyway Network PO Box 3089BroomeWA 6725Australia Australasian Wader Studies Group BroomeWA6725Australia Queensland Wader Study Group Shailer ParkQLD4128Australia Hong Kong Waterbirds ringing Group Hong Kong999077China

Many migratory birds exhibit interannual consistency in migration schedules,routes and stopover *** the interannual consistency in spatiotemporal characteristics helps understand the maintenance of migration and enables the implementation of targeted conservation *** tracked the migration of Whimbrel(Numenius phaeopus)in the East Asian-Australasian Flyway and collected spatiotemporal data from individuals that were tracked for at least two *** non-parametric tests were used to compare the interannual variations in the dates of departure from and arrival at breeding/nonbreeding sites,and the inter-annual variation in the longitudes when the same individual across the same *** exhibited a high degree of consistency in the use of breeding,nonbreeding,and stopover sites between *** variation of arrival dates at nonbreeding sites was significantly larger than that of the departure dates from nonbreeding and breeding *** used stopover sites by the same individuals in multiple years were concentrated in the Yellow Sea coast during northward migration,but were more widespread during southward *** stopover duration at repeatedly used sites was significantly longer than that at sites used only *** flying across the Yellow Sea,Whimbrels breeding in Sakha(Yakutia)exhibited the highest consistency in migration routes in both autumn and ***,the consistency in migration routes of Yakutia breeding birds was generally higher than that of birds breeding in *** results suggest that the northward migration schedule of the Whimbrels is mainly controlled by endogenous factors,while the southward migration schedule is less affected by endogenous *** repeated use of stopover sites in the Yellow Sea coast suggests this region is important for the migration of Whimbrel,and thus has high conservation value.

关键词： Consistency Migration route Migration timing Shorebird

Habitat structure directly affects aggression in convict cichlids Archocentrus nigrofasciatus

在线全文

学校读者我要写书评

暂无评论

Current Zoology 2010年第1期56卷 52-56页

作者： Anthony j. BArLEY ronald M. coleman Department of Biological Sciences California State University Sacramento California 95819-6077 USA

Aggressive behavior can be an important factor in determining how animals use and divide space and resources. Previous studies have shown that aggression in fishes can be influenced by a variety of factors, including water temperature and resource levels. In this study, we tested if the amount of habitat structure in the environment affected aggression levels in female convict cichlids Archocentrus nigrofasciatus. We performed a laboratory experiment in which we placed female convict cichlids into an aquarium with low or high amounts of habitat structure and monitored the dominant female＇s behavior toward the subordinate female. Aggressive behavior in convict cichlids primarily consists of chases and bites. We found that the total time the dominant female spent chasing the subordinate female was greater when there was a low amount of habitat structure as compared to when there was a high amount of habitat structure. We also found that both the average duration of a chasing bout and the number of bites directed at the subordinate fish increased when there was a low amount of structure, but the number of chases did not. These results indicate that increased habitat structural complexity decreases aggressive behavior in convict cichlids [Current Zoology 56 （1）： 52-56, 2010].

关键词： Neotropical Life-history Female-female competition Fish resource competition

Expanding the SDI environment:comparing current spatial data infrastructure with emerging indoor location-based services

在线全文

学校读者我要写书评

暂无评论

International journal of Digital Earth 2016年第6期9卷 629-647页

作者： David j.coleman Abbas rajabifard Kris W.Kolodziej Department of Geodesy and Geomatics Engineering University of New BrunswickFrederictonCanada Centre for Spatial Data Infrastructures and Land Administration Department of Infrastructure EngineeringThe University of MelbourneMelbourneAustralia Indoor LBS LLC New YorkNYUSA

The authors compare key elements of the emerging field of Indoor Location-Based Services(Indoor LBS)to those currently found in spatial data infrastructure(SDI)*** a brief review of SDIs and Location-Based Services,the corresponding drivers,characteristics and emerging issues within the field of Indoor LBS are introduced and discussed.A comparative framework relates the two in terms of the criteria‘People’,‘Data’,‘Technologies’,‘Standards’and‘Policies/Institutional Arrangements’.After highlighting key similarities and differences,the authors suggested three areas–definition of common framework datasets in Indoor LBS,more effective use of volunteered geographic information by SDI programs and development of appropriate privacy policies by both communities–that may benefit from sharing‘lessons learned’.

关键词： Spatial data infrastructure location-based services indoor positioning mapping geospatial

The thermal and storage stability of bovine haemoglobin by ultraviolet-visible and circular dichroism spectroscopies

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

journal of Pharmaceutical Analysis 2016年第4期6卷 242-248页

作者： ruchir Bhomia Vivek Trivedi Nichola j.coleman john C.Mitchell School of Science University of GreenwichCentral Avenue

The effects of temperature,pH and long-term storage on the secondary structure and conformation changes of bovine haemoglobin（bHb） were studied using circular dichroism（CD） and ultraviolet-visible（UV-vis） *** network software was used to deconvolute the CD data to obtain the fractional content of the five secondary *** storage stability of bHb solutions in pH 6,7 and8 buffers was significantly higher at 4 ℃ than at 23 ℃ for the first 3 days.A complete denaturation of bHb was observed after 40 days irrespective of storage temperature or *** bHb solutions were also exposed to heating and cooling cycles between 25 and 65 ℃ and structural changes were followed by UVvis and CD *** experiments demonstrated that α-helix content of bHb decreased steadily with the increasing temperature above 35 ℃ at all pH *** loss in a-helicity and gain in random coil conformations was pH-dependent and the greatest under alkaline ***,there was minimal recovery of the secondary structure content upon cooling to 25 ℃.The use of bHb as a model drug is very common and this study elucidates the significance of storage and processing conditions on its stability.

关键词： Bovine haemoglobin Circular dichroism Thermal stability Storage stability Ultraviolet-visible spectroscopy

Pancreatic cancer survival trends in the US from 2001 to 2014:a CONCOrD-3 study

在线全文

学校读者我要写书评

暂无评论

Cancer Communications 2023年第1期43卷 87-99页

作者： Maja Nikšić Pamela Minicozzi Hannah K Weir Heather Zimmerman Maria j Schymura judith r rees Michel P coleman Claudia Allemani 无 Cancer Survival Group Department of Non-Communicable Disease EpidemiologyLondon School of Hygiene&Tropical MedicineKeppel StreetLondon WC1E 7HTUK Centre for Health Services Studies University of KentCanterbury CT27NFUK Division of Cancer Prevention and Control Centers for Disease Control and PreventionAtlantaGA 30333USA Montana Central Tumor registry Chronic Disease Prevention and Health Promotion BureauPO Box 2029511400 BroadwayHelenaMT 59620-2951USA Bureau of Cancer Epidemiology New York State Cancer RegistryNew York State Department of Health150 BroadwayAlbanyNY 12204-2719USA New Hampshire State Cancer registry Norris Cotton Cancer Centerand Department of EpidemiologyGeisel School of MedicineDartmouth CollegeDartmouth-Hitchcock Medical CenterOne Medical Center DriveLebanonNH 03756USA Cancer Division University College London Hospitals NHS Foundation Trust250 Euston RoadLondon NW12PGUK US CONCOrDWorking Group

Background:Survival from pancreatic cancer is low *** the US,the 5-year relative survival has been slightly higher for women,whites and younger patients than for their counterparts,and differences in age and stage at diagnosis[Corrections added Nov 16,2022,after first online publication:a new affiliation is added to Maja Nikšić]may contribute to this *** aimed to examine trends in survival by race,stage,age and sex for adults(15-99 years)diagnosed with pancreatic cancer in the ***:This population-based study included 399,427 adults registered with pancreatic cancer in 41 US state cancer registries during 2001-2014,with followup to December 31,*** estimated age-specific and age-standardized net survival at 1 and 5 ***:Overall,12.3%of patients were blacks,and 84.2%were *** 9.5%of patients were diagnosed with localized disease,but 50.5%were diagnosed at an advanced stage;slightly more among blacks,mainly among *** substantial changes were seen over time(2001-2003,2004-2008,2009-2014).In general,1-year net survival was higher in whites than in blacks(26.1%vs.22.1%during 2001-2003,35.1%vs.31.4%during 2009-2014).This difference was particularly evident among patients with localized disease(49.6%in whites vs.44.6%in blacks during 2001-2003,60.1%vs.55.3%during 2009-2014).The survival gap between blacks and whites with localized disease was persistent at 5 years after diagnosis,and it widened over time(from 24.0%vs.21.3%during 2001-2003 to 39.7%vs.31.0%during 2009-2014).The survival gap was wider among men than among ***:Gaps in 1-and 5-year survival between blacks and whites were persistent throughout 2001-2014,especially for patients diagnosed with a localized tumor,for which surgery is currently the only treatment modality with the potential for cure.

关键词： pancreatic cancer race/ethnicity stage net survival population-based cancer registries

SOME DYNAMIC BEHAVIOUrS OF TaS3 AND ITS POSSIBLE ELECTrONIC APPLICATIONS

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Mathematics 1986年第10期29卷 1085-1095页

作者：陈倜嵘,朱维乐,P.D.coleman,j.TUCKEr Chengdu Institute of radio Engineering Chengdu Institute of radio Engineering University of Illinois U.S.A. University of Illinois U.S.A.

Some dynamic behaviours of quasi-1-dimensional conductor TaS3under charge density waves （CDW's） state have been carefully examined. The presence of hysteresis and phase shift in the response of CDW's to ac signals has been revealed for the first time. It has been demonstrated that the strong nonlinear electrical properties of CDW's can be used in electronic circuit to perform a number of nonlinear transformations, such as frequency conversion, modulation and heterodyne detection. This makes the 1-dimensional conductor a very attractive material for the bulk effect electronic device.A circuit model has been proposed to explain phenomenologically the observed effects.

关键词： SOME DYNAMIC BEHAVIOUrS OF TaS3 AND ITS POSSIBLE ELECTrONIC APPLICATIONS CDW MHz 卜尸 TTF

Maternal body condition during latepregnancy is associated with in utero development and neonatal growth of Holstein calves

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

journal of Animal Science and Biotechnology 2021年第3期12卷 984-994页

作者： A.S.Alharthi D.N.coleman I.A.Alhidary M.M.Abdelrahman E.Trevisi j.j.Loor Department of Animal Production College of Food and Agriculture SciencesKing Saud UniversityRiyadh 11451Saudi Arabia Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbanaIL 61801USA Department of Animal Sciences Food and Nutrition(DIANA)Faculty of AgricultureFood and Environmental ScienceUniversitàCattolica del Sacro Cuore29122 PiacenzaItaly

Background:Nutritional management in the dry period can alter body condition score(BCS)in dairy cows,a subjective measure of body *** such,differences in BCS during late-pregnancy not only mirror nutrient utilization by fat depots,but also can play important roles on the metabolic and hormonal *** investigated the association between cow BCS during late-pregnancy on developmental parameters and blood variables of neonatal ***-nine multiparous Holstein cows were retrospectively divided by prepartal BCS into normal BCS≤3.25(NormBCS;3.02±0.17,n=30)or high BCS≥3.75(HighBCS;3.83±0.15,n=19)*** samples were collected from cows at−10 d relative to *** weight,hip and wither height,hip width and body length were measured at birth and weekly through weaning(42 d of age)and until 9 weeks of *** blood samples were collected from the jugular vein at birth(before receiving colostrum,0 d),24 h after first colostrum and at 7,21,42 and 50 d of *** data were subjected to ANOVA using the mixed procedure of *** statistical model included day,BCS,and their ***:Dry matter intake(kg/d or%of body weight)during the last 4 weeks of pregnancy was lower(P≤0.06)in HighBCS *** concentrations of fatty acids,ceruloplasmin,and nitric oxide were greater overall(Prior to calving in HighBCS cows,and they tended(P=0.08)to have greater concentrations of reactive oxygen *** body weight was lower(P=0.03)in calves born to dams with *** addition,plasma concentrations of fatty acids,albumin and urea(Pre greater in those *** calves born to cows with HighBCS maintained a lower postnatal body weight(P=0.04),hip and wither height,hip width,and body length,there was no difference(P>0.05)in daily starter intake and average daily gain due to maternal ***:Overall,results highlight an association between BCS during late-gestation on in utero calf development and postna

关键词： Metabolism Neonate Nutritional programming Transition cow

reproductive potential of field-collected overwintering boll weevils （Coleoptera： Curculionidae） fed on pollen in the laboratory

在线全文

学校读者我要写书评

暂无评论

Insect Science 2009年第4期16卷 321-327页

作者： S. M. Greenberg G. D. jones j. j. Adamczyk, jr. F. Eischen j. S. Armstrong r. j. coleman M. Setamou Tong-Xian Liu USDA-ArS-KSArC-BIrU 2413 E. Hwy. 83 Weslaco Texas 78596 APMrU-SPA-ArS-USDA College Station Texas 77845 USDA- ArS-KSA rC-Honey Bee research Unit Texas A&M University Kingsville Citrus Center Weslaco TX 78596 Department of Entomology Texas AgriLife Research Texas A&M University System Weslaco Texas 78596 USA

The reproductive potential of overwintering boll weevil, Anthonomus grandis grandis （Boheman）, females collected from pheromone traps in September, November and january, fed for 1, 3, and 5 weeks on plant pollens, and then provided cotton squares, was determined in the laboratory at 27 ~ I^C, 65% rH, and a photoperiod 13 ： 11 （L ： D） h. Duration of pollen feeding by overwintering boll weevils did not significantly influence egg and feeding punctures, or puncture ratios （egg to total punctures） for any of the three months of parent weevil collections when provided cotton squares on a daily basis. However, punctures and puncture ratios are significantly different when comparing mean data between months of boll weevil collections. When boll weevils were provided with cotton squares daily, the pre-ovipositional periods of female parents captured in September, November and january were 5, 9 and 14 days, respectively. The rate of eggs by females was significantly lower during November and january than September. Female parents collected in September produced a significantly higher percentage of eggs yielding adult progeny than those collected in November and january. Life table parameters indicated that net reproductive rate （ro） of boll weevil females collected in September was 1.2- fold higher than those collected in November and 10.7-fold higher than those collected in january. Except for testes size, no differences in male reproductive parameters were observed during the cotton-free period compared with males captured during mid-cotton （june）. The number of oocytes in the ovarioles and the number of ooeytes containing yolk were significantly lower during September, November and january compared with june. The reproductive potential ofoverwintering boll weevil females collected in different months is an important consideration in determining the success of any control strategy.

关键词： Anthonomusgrandisgrandis overwintering boll weevil reproductive pollen