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Effect of coenzyme Q10 supplementation on post-vitrification mouse embryo development

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Asian pacific Journal of reproduction 2024年第3期13卷 126-132页

作者： Anisa-Annur s Wan-Hafizah WJ Nor-Ashikin MNK Muhammad-Zaki r Faculty of Pharmacy and Health sciences Royal College of Medicine PerakUniversiti Kuala Lumpur30450 IpohPerakMalaysia Maternofetal and Embryo research Group Faculty of MedicineUniversiti Teknologi MARASelangor BranchSungai Buloh Campus47000 Sungai BulohSelangorMalaysia

Objective:To investigate the effects of coenzyme Q10(CoQ10)supplementation on post-vitrification embryo development and gross ***:balb/c mouse embryos were cultured in potassium simplex optimised medium(KsOM)with varying CoQ10 concentrations[0(control),20,40,and 60μM].The most effective CoQ10 concentration(40μM)was selected for subsequent post-vitrification morphology *** were randomly divided into four groups:Group A(non-vitrified without CoQ10),Group b(non-vitrified with CoQ10),Group C(vitrified without CoQ10),and Group D(vitrified with CoQ10),followed by vitrification at the 8-cell *** rates and development until the blastocyst stage were evaluated through morphological examinations using AsEbIr's system,distinguishing normal and abnormal ***:supplementation of 40μM CoQ10 significantly increased blastocyst formation(95%)compared to the control group(92%),20μM(62%),and 60μM(56%)(Prification,Group D exhibited a significant increase in blastocyst formation(92%)compared to Group C(82%)(Prphological assessments indicated superior embryo quality in Group b over Group D during the cleavage stage,morula,and blastocyst(Psions:CoQ10 supplementation exhibits promising potential to enhance preimplantation embryo development,increase blastocyst formation rates,and improve embryo quality *** offers a promising approach to mitigate oxidative stress on embryos,potentially improving overall assisted reproductive technology outcomes.

关键词： Coenzyme Q10 Antioxidant Embryo development Vitrification Morphology Assisted reproductive technology

HVOF-sprayed HAp/s53P4 bG composite coatings on an AZ31 alloy for potential applications in temporary implants

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Journal of Magnesium and Alloys 2024年第1期12卷 345-360页

作者： Carlos A.Poblano-salas John Henao Astrid L.Giraldo-betancur Paola Forero-sossa Diego German Espinosa-Arbelaez Jorge A.González-sánchez Luis r.Dzib-Pérez susana T.Estrada-Moo Idelfonso E.Pech-Pech CIATEQ A.C. Av.Manantiales 23-AParque Industrial Bernardo QuintanaEl MarquésQuerétaro 76246Mexico CONAHCYT-CIATEQ A.C. Av.Manantiales 23-AParque Industrial Bernardo QuintanaEl MarquésQuerétaro 76246Mexico CONAHCYT-Cinvestav Libramiento Norponiente#2000.Fraccionamiento Real de JuriquillaQuerétaro 76230Mexico Universidad Tecnológica de Querétaro Av.Pie de la Cuesta N°2501Querétaro 76148Mexico CIDEsI Av.Playa Pie de la Cuesta No 702Col.San PabloQuerétaro 76125Mexico Center for Corrosion research Autonomous University of CampecheAv.Héroe de Nacozari No.480P.C.24070San Francisco de CampecheCam.Mexico

bioactive thermal spray coatings produced via high-velocity oxygen fuel spray(HVOF)from hydroxyapatite(HAp)and bioactive glasses(bG)have the potential to be employed on temporary implants due to the ability of both HAp and bG to dissolve and promote osseointegration,considering that both phases have different reaction and dissolution rates under in-vitro *** the present work,75%***-25%wt.s53P4 bioactive glass powders were HVOF-sprayed to obtain HAp/s53P4 bG composite coatings on a bioresorbable AZ31 *** study is focused on exploring the effect of the stand-off distance and fuel/oxygen ratio variation as HVOF parameters to obtain stable structural coatings and to establish their effect on the phases and microstructure produced in those *** characterization techniques,such as scanning electron microscopy,X-ray diffraction,and Fourier transform infrared spectroscopy,were employed to characterize relevant structural and microstructural properties of the composite *** results showed that thermal gradients during coating deposition must be managed to avoid delamination due to the high temperature achieved(max 550℃)and the differences in coefficients of thermal *** was also found that both spraying distance and oxygen/fuel ratio allowed to keep the hydroxyapatite as the main phase in the *** addition,in-vitro electrochemical studies were performed on the obtained HAp/s53P4 bG composite coatings and compared against the uncoated AZ31 *** results showed a significant decrease in hydrogen evolution(at least 98%)when the bioactive coating was applied on the Mg alloy during evaluation in simulated body fluid(sbF).

关键词： Coatings Composites Thermal spray Temporary implants Hydrogen evolution

structural effects induced by dialysis-based purification of carbon nanomaterials

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Nano Materials science 2024年第4期6卷 475-483页

作者： A.D.Veloso A.M.Ferraria A.M.botelho do rego A.s.Viana A.J.s.Fernandes A.J.Fielding r.A.Videira M.C.Oliveira CQ-Vr and Chemistry Department University of Trás-os-Montes e Alto Douro bsIrG iBB-Institute for Bioengineering and BiosciencesDepartment of Chemical Engineeringand Associate Laboratory i4HB-Institute for Health and BioeconomyInstituto Superior TécnicoUniversidade de Lisboa Centro de Química Estrutural Institute of Molecular SciencesDepartamento de Química e BioquímicaFaculdade de CiênciasUniversidade de Lisboa I3N and Physics Department University of AveiroCampus de Santiago Faculty of science Pharmacy and Biomolecular SchoolLiverpool John Moores University rEQUIMTE/LAQV Laborat orio de FarmacognosiaDepartamento de QuímicaFaculdade de Farm aciaUniversidade do Porto

Dialysis plays a crucial role in the purification of nanomaterials but its impact on the structural properties of carbon nanomaterials was never ***,a carbon-based nanomaterial generated electrochemically in potassium phosphate buffer,was characterized before and after dialysis against pure *** is shown that dialysis affects the size of the carbon domains,structural organization,surface functionalization,oxidation degree of carbon,and grade of ***,dialysis drives the nanomaterial organization from discrete roundish carbon domains,with sizes ranging from 70 to 160 nm,towards linear stacking structures of small nanoparticles （rallel,alcohol and ether （epoxide） surface groups evolve into more oxidized carbon groups （e.g.,ketone and ester groups）.Investigation of the as-prepared nanomaterial by electron paramagnetic resonance （EPr） revealed a resonance signal consistent with carbon-oxygen centred ***,this study brings to light the selective affinity of the carbon nanomaterial under study to capture Na+ions,a property greatly enhanced by the dialysis process,and its high ability to trap oxygen,particularly before *** findings open new perspectives for the application of carbon-based nanomaterials and raise awareness of the importance of structural changes that can occur during the purification of carbon-based nanomaterials.

关键词： Nanocarbon surface functionalization NMr XPs Hydrophilic carbon Paramagnetic

Multidimensional images and aberrations in sTEM

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Chinese Physics b 2024年第9期33卷 68-74页

作者： Eric r.Hoglund Andrew r.Lupini Center for Nanophase Materials sciences Oak Ridge National LaboratoryOak RidgeTN 37831United States

recent advances in scanning transmission electron microscopy(sTEM)have led to increased development of multidimensional sTEM imaging modalities and novel image reconstruction *** interest arises because the main electron lens in a modern transmission electron microscope usually has a diffraction-space information limit that is significantly better than the real-space resolution of the same *** state-of-affairs is sometimes shared by other scattering methods in modern physics and contributes to a broader excitement surrounding multidimensional techniques that scan a probe while recording diffraction-space images,such as ptychography and scanning nano-beam ***,the contrasting resolution in the two spaces raises the question as to what is limiting their effective ***,we examine this paradox by considering the effects of aberrations in both image and diffraction planes,and likewise separate the contributions of pre-and post-sample *** consideration provides insight into aberration-measurement techniques and might also indicate improvements for super-resolution techniques.

关键词： scanning transmission electron microscopy (sTEM) aberrations

Laboratory evaluation of toxicity of selected insecticides against egg and larval stages of cotton pink bollworm, Pectinophora gossypiella (saunders) (Lepidoptera: Gelechiidae)

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Journal of Cotton research 2024年第1期7卷 15-28页

作者： bUsNOOr Abhishek V. WADAsKAr r.M. FAND babasaheb b. TAMbE V.J. PILLAI T. MAHULE D.J. NAGrArE V.s. PrAsAD Y.G. Entomology section College of Agriculture(Dr.Panjabrao Deshmukh Krishi Vidyapeeth)Nagpur 440001MaharashtraIndia Indian Council of Agricultural research-Central Institute for Cotton research Nagpur 441108MaharashtraIndia

background The cryptic nature of pink bollworm Pectinophora gossypiella(saunders)larvae enables its reduced vul-nerability to insecticidal ***,the development of resistance against bacillus thuringiensis(bt)toxins posed a serious threat to transgenic cotton *** necessitated determining the critical timing of spray applications on the control *** study assessed the influence of egg age(freshly laid ***-day-old)and the loca-tion of larvae(directly exposed to the insecticide residues on the boll rind *** inside the bolls)on insecticide control *** The results revealed a significant decrease in the ovicidal activity of tested insecticides with an increase in the age of eggs from one day old to three days old(paired t-test,Prvae directly exposed to the insec-ticide residues on the boll rind were more susceptible(>80%mortality)than the larvae exposed after they had bur-rowed inside the bolls(rtality).The inhibitory effects of tested insecticides on developmental biology were more pronounced in the experiment on pre-larval release insecticide treatment compared with insecticide treatment given post-larval release and entry inside the *** Egg age influences the insecticide susceptibility,as does the larval location,directly exposed vs bur-rowed inside the *** eggs and the larvae that had burrowed inside the green bolls of cotton were relatively less susceptible to the insecticide *** toxic effects of insecticides on egg and larval stages were primar-ily *** findings are significant for devising a comprehensive strategy for pink bollworm management on a sustainable basis.

关键词： bioefficacy Cotton Insecticides Pink bollworm Pectinophora gossypiella Timings of spray

The autophagy protein Atg9 functions in glia and contributes to parkinsonian symptoms in a Drosophila model of Parkinson’s disease

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Neural regeneration research 2024年第5期19卷 1150-1155页

作者： shuanglong Yi Linfang Wang Margaret s.Ho shiping Zhang school of Life science and Technology ShanghaiTech UniversityShanghaiChina College of Life sciences University of Chinese Academy of SciencesBeijingChina

Parkinson’s disease is a progressive neurodegenerative disease characterized by motor deficits,dopaminergic neuron loss,and brain accumulation ofα-synuclein aggregates called Lewy *** in protein degradation pathways,such as autophagy,has been demonstrated in neurons as a critical mechanism for eliminating protein aggregates in Parkinson’s ***,it is less well understood how protein aggregates are eliminated in glia,the other cell type in the *** the present study,we show that autophagy-related gene 9(Atg9),the only transmembrane protein in the autophagy machinery,is highly expressed in Drosophila glia from adult *** from immunostaining and live cell imaging analysis reveal that a portion of Atg9 localizes to the trans-Golgi network,autophagosomes,and lysosomes in ***9 is persistently in contact with these *** glial atg9 reduces the number of omegasomes and autophagosomes,and impairs autophagic substrate *** suggests that glial Atg9 participates in the early steps of autophagy,and hence the control of autophagic ***,loss of glial atg9 induces parkinsonian symptoms in Drosophila including progressive loss of dopaminergic neurons,locomotion deficits,and glial *** findings identify a functional role of Atg9 in glial autophagy and establish a potential link between glial autophagy and Parkinson’s *** results may provide new insights on the underlying mechanism of Parkinson’s disease.

关键词： Atg9 autophagy glia Parkinson’s disease

基于机器学习方法的短期太阳爆发活动预报模型:综述

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中国科学:地球科学 2024年

作者：黄鑫赵忠瑞钟昱丰徐龙 Marianna b.KOrsós r.ErDéLYI 宁波大学信息科学与工程学院中国科学院空间天气学国家重点实验室国家空间科学中心中国科学院大学天文与空间科学学院北京元胞探索科技中心 Dipartimento di Fisica e Astronomia“Ettore Majorana” Universitàdi Catania Department of Astronomy E?tv?s Loránd University Hungarian solar Physics Foundation solar Physics&space Plasma research Center (sP2rC) School of Mathematics and StatisticsUniversity of Sheffield

太阳爆发活动主要包括太阳耀斑、日冕物质抛射(coronal mass ejections, CME)和太阳质子事件(solar proton events, sPE),太阳爆发活动对空间天气和高技术领域有重要影响.太阳爆发活动短期预报是空间天气预报中一个活跃的研究领域.目前... 详细信息

太阳爆发活动主要包括太阳耀斑、日冕物质抛射(coronal mass ejections, CME)和太阳质子事件(solar proton events, sPE),太阳爆发活动对空间天气和高技术领域有重要影响.太阳爆发活动短期预报是空间天气预报中一个活跃的研究领域.目前,数值的、统计的和机器学习的方法被用来建立太阳爆发活动预报模型.随着天基和地基观测设备的发展,积累了大量的太阳观测数据,数据驱动的太阳爆发活动预报模型取得了重大进展.本文介绍了机器学习算法在太阳爆发活动预报中的应用,总结了预报建模过程,概述了太阳爆发活动预报模型的进展,并展望了未来可能的研究方向.

关键词：太阳耀斑日冕物质抛射太阳质子事件机器学习预报模型

Performance and safety Improvement of Induction Motors based on Testing and Evaluation standards

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CEs Transactions on Electrical Machines and systems 2024年第3期8卷 310-318页

作者： Nkosinathi s.Khumalo Ntombizotwa P.Memane Udochukwu b.Akuru Department of Electrical Engineering Tshwane University of TechnologyPretoria 0183South Africa

The induction motor,which converts electrical energy into mechanical energy,has been recognized as the cornerstone of *** rotor of an induction motor can be either a squirrel cage rotor or a wound-type rotor,both existing as magnetless ***-phase squirrel cage induction motors are frequently utilized in industrial drives because they are dependable,have high starting torque,are selfstarting and ***-phase induction motors,on the other hand,are commonly used for small loads such as domestic appliances in form of modest fans,pumps and electric power *** south Africa,there have been reports of fires and explosions resulting in live and property loss because of induction motors that have not been thoroughly tested or are incorrectly labelled in terms of ratings,electrical safety and *** goal of this study is targeted at preventing end-user injuries and failures caused by non-compliant induction motors,by evaluating locally manufactured/imported induction motors based on tests and evaluation from standards(IEC and sANs).The study is conducted using experimental procedures at the Explosion Prevention Technology and rotating Machines(EPT and rM)laboratory,south African bureau of standards(sAbs),south *** main finding from the study shows differences in the nameplate characteristics of various induction motors which could have detrimental effects such as production and operational downtime in their end-use industries,at later stages.

关键词： Compliance Induction motors International Electro-Technical standards(IEC) Nameplate Experimental tests south African National standards(sANs)

Characterization of early maturing elite genotypes based on MTsI and MGIDI indexes:an illustration in upland cotton(Gossypium hirsutum L.)

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Journal of Cotton research 2024年第3期7卷 253-265页

作者： D s rAJ supritha PATIL rajesh s. PATIL bhuvaneshwara r. NAYAK spurthi N. PAWAr Kasu N. Department of Genetics and Plant breeding College of AgricultureUniversity of Agricultural SciencesDharwad 580005India Agricultural research station Dharwad 580005India Department of biotechnology College of AgricultureUniversity of Agricultural SciencesDharwad 580005India Department of Crop Physiology College of AgricultureUniversity of Agricultural SciencesDharwad 580005India

background Globally,the cultivation of cotton is constrained by its tendency for extended periods of *** maturity plays a potential role in rainfed-based multiple cropping system especially in the current era of climate *** the current study,a set of 20 diverse Gossypium hirsutum genotypes were evaluated in two crop seasons with three planting densities and assessed for 11 morphological traits related to early *** study aimed to identify genotype(s)that mature rapidly and accomplish well under diverse environmental conditions based on the two robust multivariate techniques called multi-trait stability index(MTsI)and multi-trait genotype-ideotype distance index(MGIDI).results MTsI analysis revealed that out of the 20 genotypes,three genotypes,viz.,NNDC-30,A-2,and s-32 accomplished well in terms of early maturity traits in two ***,three genotypes were selected using MGIDI method for each planting densities with a selection intensity of 15%.The strengths and weaknesses of the genotypes selected based on MGIDI method highlighted that the breeders could focus on developing early-maturing genotypes with specific traits such as days to first flower and boll *** selected genotypes exhibited positive genetic gains for traits related to earliness and a successful harvest during the first and second ***,there were negative gains for traits related to flowering and boll *** The study identified three genotypes exhibiting early maturity and accomplished well under different planting *** multivariate methods(MTsI and MGIDI)serve as novel approaches for selecting desired genotypes in plant breeding programs,especially across various growing *** methods offer exclusive benefits and can easily construe and minimize multicollinearity issues.

关键词： Cotton MTsI MGIDI Genotype environment interaction Early maturity Multi-trait Multi-environment