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Overexpression a Alternative Oxidase Gene OsAOX1a Improves Cold Tolerance in Oryza sativa L.

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Overexpression a Alternative Oxidase Gene OsAOX1a Improves C...

Optimization of performance of the KM2A full array using the Crab Nebula

生态文明建设中的植物学：现在与未来——中国植物学会第十五届会员代表大会暨八十周年学术年会

作者： C.r.Li D.D.Liang r.f.xu H.Li Y.P.Zhang r.Y.Qin L.Li P.C.Wei J.B.Yang College of Life Sciences Anhui University Key Laboratory of rice Genetics Breeding of Anhui Province Rice Research InstituteAnhui Academy of Agricultural Sciences Institute of Agricultural Engineering Anhui Academy of Agricultural Sciences

Low temperature is a major environmental stress in rice cultivating and ***,AOX1 is an important family in gene engineering to adapt ***,the related reports were mostly focused on dicot plants Arabidopsis and tobacco,while the functional research on rice was *** this study,we cloned a rice predominant cold-response AOX1 gene,*** transgenic rice plants with OsAOX1a overexpression were *** found the OsAOX1a overexpression could strongly enhanced the cold growth of seedling,especially the root ***,the growth were not changed under normal ***,the lipid peroxidation and ion leakage rate were determined after cold treatment in transgenic *** indictors was reduced by OsAOX1a overexpression,which revealed that OsAOX1a could reduce the oxidative damage under cold *** together,our results suggested overexpressing OsAOX1a gene could improve the growth performance under cold,which might closely related to the reduction of rOS generation and oxidative damage.

关键词： Alternative Oxidase rice Cold OsAOX1a Oxidative Damage

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Chinese Physics C 2024年第6期48卷 169-178页

作者：曹臻 f.Aharonian 安琪阿西克古白云翔包逸炜 D.Bastieri 毕效军毕玉江蔡金庭曹晴曹文羽曹喆常进常劲帆陈尚明陈恩生陈亮陈林陈龙陈明君陈玛丽陈起辉陈素弘陈天禄陈阳程宁程耀东崔明阳崔树旺崔晓红崔昱东戴本忠代洪亮戴子高单增罗布 D.della Volpe 董绪强段凯凯樊军辉范一中方军方堃冯存峰封莉冯少辉丰晓婷冯有亮 S.Gabici 高博高川东高林青高启高卫高伟康葛茂茂耿利斯 G.Giacinti 龚光华苟全补顾旻皓郭福来郭晓磊郭义庆郭莹莹韩毅昂何会海贺昊宁何佳银何新波何钰 M.Heller 贺远强侯博文侯超侯贤胡红波胡铨胡世聪黄代绘黄天奇黄文俊黄性涛黄晓渊黄勇黄志成季筱璐贾焕玉贾康江琨姜晓巍姜泽军金敏康明铭柯通 D.Kuleshov.K.Kurinov 李兵兵李澄李骢李丹李飞李海波李会财李华阳李军李剑李捷李凯李文龙李文莲李秀荣李昕李一卓李哲黎卓梁恩维梁云峰林苏杰刘冰刘成刘栋刘虎刘海东刘佳刘江来刘金艳刘茂元柳若愚刘四明刘伟刘怡刘以农鲁睿罗晴吕洪魁马伯强马玲玲马欣华毛基荣闵振 W.Mitthumsiri 穆慧君南云程 A.Neronov 区子维庞彬宇 P.Pattarakijwanich 裴致远齐孟尧祁业情乔冰强秦家军 D.ruffolo A.Sáiz D.Semikoz 邵澄宇邵琅 O.Shchegolev 盛祥东舒富文宋慧超 Yu.V.Stenkin V.Stepanov 苏扬孙秦宁孙晓娜孙志斌谭柏轩唐庆文唐泽波田文武王超王昌贝王广威王洪光王惠惠王建成汪凯王利苹王玲玉王培汉王冉王为王祥高王祥玉王阳王玉东王岩谨王忠海王仲翔王振王铮韦大明魏俊杰魏永健文韬吴超勇吴含荣武莎吴雪峰吴雨生席邵强夏捷夏君集项光漫肖迪泫肖刚辛广广辛玉良邢祎熊峥徐东莲徐仁峰徐仁新徐伟立薛良闫大海颜景志颜田杨朝文杨帆杨冯帆杨何文杨佳盈杨莉莉杨明洁杨睿智杨深邦姚玉华姚志国叶一锰尹丽巧尹娜游晓浩游智勇于艳红袁强岳华曾厚敦曾婷轩曾玮查敏张彬彬张丰张海明张恒英张建立张丽霞张力张鹏飞张佩佩张瑞张少博张少如张寿山张潇张笑鹏张云峰张毅张勇赵兵赵静赵雷赵立志赵世平郑福周斌周浩周佳能周猛周平周荣周勋秀祝成光祝凤荣朱辉朱科军左雄 LHAASO Collaboration Key Laboratory of Particle Astrophyics&Experimental Physics Division&Computing Center Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China Tianfu Cosmic ray research Center Chengdu 610000China Dublin Institute for Advanced Studies 31 Fitzwilliam Place2 DublinIreland Max-Planck-Institut for Nuclear Physics P.O.Box 10398069029 HeidelbergGermany State Key Laboratory of Particle Detection and Electronics China University of Science and Technology of China Hefei 230026China School of Physical Science and Technology&School of Information Science and Technology Southwest Jiaotong UniversityChengdu 610031China School of Astronomy and Space Science Nanjing UniversityNanjing 210023China Center for Astrophysics Guangzhou UniversityGuangzhou 510006China Hebei Normal University Shijiazhuang 050024China Key Laboratory of Dark Matter and Space Astronomy&Key Laboratory of radio Astronomy Purple Mountain ObservatoryChinese Academy of SciencesNanjing 210023China Tsung-Dao Lee Institute&School of Physics and Astronomy Shanghai Jiao Tong UniversityShanghai 200240China Key Laboratory for research in Galaxies and Cosmology Shanghai Astronomical ObservatoryChinese Academy of SciencesShanghai 200030China Key Laboratory of Cosmic rays(Tibet University) Ministry of EducationLhasa 850000TibetChina National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China School of Physics and Astronomy(Zhuhai)&School of Physics(Guangzhou)&Sino-french Institute of Nuclear Engineering and Technology(Zhuhai) Sun Yat-sen UniversityZhuhai 519000&Guangzhou 510275China School of Physics and Astronomy Yunnan UniversityKunming 650091China Département de Physique Nucléaire et Corpusculaire Facultéde SciencesUniversitéde Genève24 Quai Ernest Ansermet1211 GenevaSwitzerland Institute of frontier and Interdisciplinary Science Shandong UniversityQingdao 266237China APC Universit'e Paris Cit'eCNRS/IN2P3CEA/IRFUObservat

The full array of the Large High Altitude Air Shower Observatory(LHAASO)has been in operation since July *** its kilometer-square array(KM2A),we optimized the selection criteria for very high and ultrahigh energyγ-rays using data collected from August 2021 to August 2022,resulting in an improvement in significance of the detection in the Crab Nebula of approximately 15%,compared with that of previous *** the implementation of these new selection criteria,the angular resolution was also significantly improved by approximately 10%at tens of *** aspects of the full KM2A array performance,such as the pointing error,were also calibrated using the Crab *** resulting energy spectrum of the Crab Nebula in the energy range of 10-1000 TeV are well fitted by a log-parabola model,which is consistent with the previous results from LHAASO and other experiments.

关键词： γ-ray Crab Nebula significance

LHAASO观测发现W51区域粒子能量加速至PeV的证据（英文）

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Science Bulletin 2024年第18期 2833-2841页

作者： Zhen Cao f.Aharonian Axikegu Y.X.Bai Y.W.Bao D.Bastieri X.J.Bi Y.J.Bi W.Bian A.V.Bukevich Q.Cao W.Y.Cao Zhe Cao J.Chang J.f.Chang A.M.Chen E.S.Chen H.X.Chen Liang Chen Lin Chen Long Chen M.J.Chen M.L.Chen Q.H.Chen S.Chen S.H.Chen S.Z.Chen T.L.Chen Y.Chen N.Cheng Y.D.Cheng M.Y.Cui S.W.Cui X.H.Cui Y.D.Cui B.Z.Dai H.L.Dai Z.G.Dai Danzengluobu X.Q.Dong K.K.Duan J.H.fan Y.Z.fan J.fang J.H.fang Kun fang C.f.feng H.feng L.feng S.H.feng X.T.feng Y.feng Y.L.feng S.Gabici B.Gao C.D.Gao Q.Gao W.Gao W.K.Gao M.M.Ge L.S.Geng G.Giacinti G.H.Gong Q.B.Gou M.H.Gu f.L.Guo X.L.Guo Y.Q.Guo Y.Y.Guo Y.A.Han M.Hasan H.H.He H.N.He J.Y.He Y.He Y.K.Hor B.W.Hou C.Hou X.Hou H.B.Hu Q.Hu S.C.Hu D.H.Huang T.Q.Huang W.J.Huang X.T.Huang X.Y.Huang Y.Huang X.L.Ji H.Y.Jia K.Jia K.Jiang X.W.Jiang Z.J.Jiang M.Jin M.M.Kang I.Karpikov D.Kuleshov K.Kurinov B.B.Li C.M.Li Cheng Li Cong Li D.Li f.Li H.B.Li H.C.Li Jian Li Jie Li K.Li S.D.Li W.L.Li W.L.Li X.r.Li Xin Li Y.Z.Li Zhe Li Zhuo Li E.W.Liang Y.f.Liang S.J.Lin B.Liu Cheng Liu D.Liu D.B.Liu H.Liu H.D.Liu J.Liu J.L.Liu M.Y.Liu r.Y.Liu S.M.Liu W.Liu Y.Liu Y.N.Liu Q.Luo Y.Luo H.K.Lv B.Q.Ma L.L.Ma X.H.Ma J.r.Mao Z.Min W.Mitthumsiri H.J.Mu Y.C.Nan A.Neronov L.J.Ou P.Pattarakijwanich Z.Y.Pei J.C.Qi M.Y.Qi B.Q.Qiao J.J.Qin A.raza D.ruffolo A.Sáz M.Saeed D.Semikoz L.Shao O.Shchegolev X.D.Sheng f.W.Shu H.C.Song Yu.V.Stenkin V.Stepanov Y.Su D.X.Sun Q.N.Sun X.N.Sun Z.B.Sun J.Takata P.H.T.Tam Q.W.Tang r.Tang Z.B.Tang W.W.Tian C.Wang C.B.Wang G.W.Wang H.G.Wang H.H.Wang J.C.Wang Kai Wang Kai Wang L.P.Wang L.Y.Wang P.H.Wang r.Wang W.Wang X.G.Wang X.Y.Wang Y.Wang Y.D.Wang Y.J.Wang Z.H.Wang Z.X.Wang Zhen Wang Zheng Wang D.M.Wei J.J.Wei Y.J.Wei T.Wen C.Y.Wu H.r.Wu Q.W.Wu S.Wu X.f.Wu Y.S.Wu S.Q.Xi J.Xia G.M.Xiang D.X.Xiao G.Xiao Y.L.Xin Y.Xing D.r.Xiong Z.Xiong D.L.xu r.f.xu r.X.xu W.L.xu L.xue D.H.Yan J.Z.Yan T.Yan C.W.Yang C.Y.Yang f.Yang f.f.Yang L.L.Yang M.J.Yang r.Z.Yang W.X.Yang Y.H.Yao Z.G.Yao L.Q.Yin N.Yin X.H.You Z.Y.You Y.H.Yu Q.Yuan H.Yue H.D.Zeng T.X.Zeng W.Zeng M.Zha B.B.Zhang f.Zhang H.Zhang H.M.Zhang H.Y.Z LHAASO合作组 Key Laboratory of Particle Astrophysics & Experimental Physics Division & Computing Center Institute of High Energy Physics Chinese Academy of Sciences University of Chinese Academy of Sciences TIANfU Cosmic ray research Center Dublin Institute for Advanced Studies Max-Planck-Institut for Nuclear Physics School of Physical Science and Technology & School of Information Science and Technology Southwest Jiaotong University School of Astronomy and Space Science Nanjing University Center for Astrophysics Guangzhou University Tsung-Dao Lee Institute & School of Physics and Astronomy Shanghai Jiao Tong University Institute for Nuclear research of russian Academy of Sciences Hebei Normal University University of Science and Technology of China State Key Laboratory of Particle Detection and Electronics China Key Laboratory of Dark Matter and Space Astronomy & Key Laboratory of radio Astronomy Purple Mountain Observatory Chinese Academy of Sciences research Center for Astronomical Computing Zhejiang Laboratory Key Laboratory for research in Galaxies and Cosmology Shanghai Astronomical Observatory Chinese Academy of Sciences School of Physics and Astronomy Yunnan University Key Laboratory of Cosmic rays (Tibet University) Ministry of Education National Astronomical Observatories Chinese Academy of Sciences School of Physics and Astronomy (Zhuhai) & School of Physics(Guangzhou) & Sino-french Institute of Nuclear Engineering and Technology (Zhuhai) Sun Yat-sen University Institute of frontier and Interdisciplinary Science Shandong University APC UniversitéParis Cité CNrS/IN2P3 CEA/IrfU Observatoire de Paris Department of Engineering Physics Tsinghua University School of Physics and Microelectronics Zhengzhou University Yunnan Observatories Chinese Academy of Sciences China Center of Advanced Science and Technology College of Physics Sichuan University School of Physics Peking University Guangxi Key Laboratory for relativistic Astrophysics School of Physical Science and Technology Guangxi University Department of Ph

The γ-ray emission from the W51 complex is widely acknowledged to be attributed to the interaction between the cosmic rays （Crs） accelerated by the shock of supernova remnant （SNr） W51C and the dense molecular clouds in the adjacent star-forming region,***,the maximum acceleration capability of W51C for Crs remains *** on observations conducted with the Large High Altitude Air Shower Observatory （LHAASO）,we report a significant detection of γ rays emanating from the W51 complex,with energies from 2 to 200 *** LHAASO measurements,for the first time,extend the γ-ray emission from the W51 complex beyond 100 TeV and reveal a significant spectrum bending at tens of *** combining the"π0-decay bump"featured data from fermi-LAT,the broadband γ-ray spectrum of the W51 region can be well-characterized by a simple pp-collision *** observed spectral bending feature suggests an exponential cutoff at～400 TeV or a power-law break at～200 TeV in the Cr proton spectrum,most likely providing the first evidence of SNrs serving as Cr accelerators approaching the PeV ***,two young star clusters within W51B could also be theoretically viable to produce the most energetic γ rays observed by *** findings strongly support the presence of extreme Cr accelerators within the W51 complex and provide new insights into the origin of Galactic Crs.

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Electrochemical realization of 3D Interconnected MoS_(3)/PPy Nanowire frameworks as Sulfur-Equivalent Cathode Materials for Li-S Batteries

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Energy & Environmental Materials 2024年第2期7卷 9-17页

作者： Hongtao Yu Andreas Siebert Shilin Mei raul Garcia-Diez roberto félix Ting Quan Yaolin xu Johannes frisch regan G.Wilks Marcus Bär Chun Pei Yan Lu Department for Electrochemical Energy Storage Helmholtz-Zentrum Berlin für Materialien und Energie GmbH(HZB)Hahn-Meitner Platz 114109 BerlinGermany Guangdong Province Key Laboratory of Durability for Marine Civil Engineering School of Civil EngineeringShenzhen UniversityShenzhenGuangdong 518060China Department of Interface Design HZBAlbert-Einstein-Str.1512489 BerlinGermany Energy Materials In-Situ Laboratory Berlin(EMIL) Albert-Einstein-Str.1512489 BerlinGermany Helmholtz-Institute Erlangen-Nürnberg for renewable Energy(HI ErN) Albert-Einstein-Str.1512489 BerlinGermany Department of Chemistry and Pharmacy Friedrich-Alexander-Universität Erlangen-NürnbergEgerlandstr.391058Erlangen Germany Institute of Chemistry University of Potsdam14467Potsdam Germany

The development of freestanding and binder-free electrode is an effective approach to perform the inherent capacity of active materials and promote the mechanism study by minimizing the interference from ***,we construct a freestanding cathode composed of MoS_(3)/PPy nanowires(NWs)deposited on porous nickel foam(Nf)(MoS_(3)/PPy/Nf)through electrochemical methods,which can work efficiently as sulfur-equivalent cathode material for Li-S *** structural stability of the MoS_(3)/PPy/Nf cathode is greatly enhanced due to its significant tolerance to the volume expansion of MoS_(3)during the lithiation process,which we ascribe to the flexible 3D framework of PPy NWs,leading to superior cycling performance compared to the bulk-MoS_(3)/Nf *** the interference of binder and carbon additives,the evolution of the chemical and electronic structure of Mo and S species during the discharge/charge was studied by X-ray absorption near-edge spectroscopy(XANES).The formation of lithium polysulfides was excluded as the driving cathode reaction mechanism,suggesting the great potential of MoS_(3)as a promising sulfur-equivalent cathode material to evade the shuttle effect for Li-S *** present study successfully demonstrates the importance of structural design of freestanding electrode enhancing the cycling performances and revealing the corresponding mechanisms.

关键词： 3D-Network electrochemical lithium-sulfur battery MoS_(3) PPy

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Layered Potassium Titanium Niobate/reduced Graphene Oxide Nanocomposite as a Potassium‑Ion Battery Anode

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Nano-Micro Letters 2024年第1期16卷 1-16页

作者： Charlie A.f.Nason Ajay Piriya Vijaya Kumar Saroja Yi Lu runzhe Wei Yupei Han Yang xu Department of Chemistry University College London20 Gordon StreetLondon WC1H 0AJUK

With graphite currently leading as the most viable anode for potassium-ion batteries(KIBs),other materials have been left relatively *** metal oxides are among these,with many positive attributes such as synthetic maturity,longterm cycling stability and fast redox ***,to address this research deficiency we report herein a layered potassium titanium niobate KTiNbO5(KTNO)and its rGO nanocomposite(KTNO/rGO)synthesised via solvothermal methods as a high-performance anode for *** effective distribution across the electrically conductive rGO,the electrochemical performance of the KTNO nanoparticles was *** potassium storage performance of the KTNO/rGO was demonstrated by its first charge capacity of 128.1 mAh g^(−1) and reversible capacity of 97.5 mAh g^(−1) after 500 cycles at 20 mA g^(−1),retaining 76.1%of the initial capacity,with an exceptional rate performance of 54.2 mAh g^(−1)at 1 A g^(−1).furthermore,to investigate the attributes of KTNO in-situ XrD was performed,indicating a low-strain ***-situ X-ray photoelectron spectra further investigated the mechanism of charge storage,with the titanium showing greater redox reversibility than the *** work suggests this lowstrain nature is a highly advantageous property and well worth regarding KTNO as a promising anode for future high-performance KIBs.

关键词： Potassium-ion batteries Intercalation Transition metal oxides Anodes Nanocomposite

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DCVAE-adv:A Universal Adversarial Example Generation Method for White and Black Box Attacks

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Tsinghua Science and Technology 2024年第2期29卷 430-446页

作者： Lei xu Junhai Zhai College of Mathematics and Information Science Hebei UniversityBaoding 071002China

Deep neural network(DNN)has strong representation learning ability,but it is vulnerable and easy to be fooled by adversarial *** order to handle the vulnerability of DNN,many methods have been *** general idea of existing methods is to reduce the chance of DNN models being fooled by observing some designed adversarial examples,which are generated by adding perturbations to the original *** this paper,we propose a novel adversarial example generation method,called *** from the existing methods,DCVAE-adv constructs adversarial examples by mixing both explicit and implicit perturbations without using original ***,the proposed method can be applied to both white box and black box *** addition,in the inference stage,the adversarial examples can be generated without loading the original images into memory,which greatly reduces the memory *** compared DCVAE-adv with three most advanced adversarial attack algorithms:fGSM,AdvGAN,and AdvGAN++.The experimental results demonstrate that DCVAE-adv is superior to these state-of-the-art methods in terms of attack success rate and transfer ability for targeted *** code is available at https://***/xzforeverlove/DCVAE-adv.

关键词： deep neural network adversarial examples white box attack black box attack robustness

Experimental investigation on the permeability of gap-graded soil due to horizontal suffusion considering boundary effect

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Journal of rock Mechanics and Geotechnical Engineering 2024年第3期16卷 1072-1084页

作者： xuwei Wang Yeshuang xu State Key Laboratory of Ocean Engineering School of Naval ArchitectureOceanand Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China Shanghai Key Laboratory for Digital Maintenance of Buildings and Infrastructure Department of Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China

The boundary condition is a crucial factor affecting the permeability variation due to *** experimental investigation on the permeability of gap-graded soil due to horizontal suffusion considering the boundary effect is conducted,where the hydraulic head difference(DH)varies,and the boundary includes non-loss and soil-loss *** samples are filled into seven soil storerooms connected in *** evaluation,the variation in content of fine sand(Δr_(f))and the hydraulic conductivity of soils in each storeroom(C_(i))are *** the non-loss test,the soil sample filling area is divided into runoff,transited,and accumulated areas according to the negative or positive Δr_(f) values.Δr_(f) increases from negative to positive along the seepage path,and Ci decreases from runoff area to transited area and then rebounds in accumulated *** the soil-loss test,all soil sample filling areas belong to the runoff area,where the gentle-loss,strengthened-loss,and alleviated-loss parts are further divided.Δr_(f) decreases from the gentle-loss part to the strengthened-loss part and then rebounds in the alleviated-loss part,and C_(i) increases and then decreases along the seepage *** relationship between Δr_(f) and Ci is different with the boundary *** exponentially decreases with Δr_(f) in the non-loss test and increases with Δr_(f) generally in the soil-loss test.

关键词： Suffusion Permeability Experimental investigation Boundary effect Horizontal seepage

Periodic Variation Studies of the Two Short Period W UMa-type Eclipsing Binaries: LX Lyn and V0853 Aur

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research in Astronomy and Astrophysics 2024年第1期24卷 273-284页

作者： xu Zhang Bin Zhang School of Physics and Electronic Science Guizhou Normal UniversityGuiyang 550001China Guizhou Provincial Key Laboratory of radio Astronomy and Data Processing Guizhou Normal UniversityGuiyang 550025China

In this paper,new light curves(LCs) of contact eclipsing binary(CEB) systems LX Lyn and V0853 Aur are presented and analyzed by using the 2015 version of the Wilson-Devinney(W-D) *** order to explain their asymmetric LCs,cool starspots on the components were *** is suggested that their fill-out degrees are f=12.0%(LX Lyn) and f=26.3%(V0853 Aur).At the same time,we found that LX Lyn is a W-type eclipsing binary(EB) with an orbital inclination of i=84°.88 and a mass ratio of q=2.31.V0853 Aur is also a W-type CEB with a mass ratio of q=2.77 and an orbital inclination of i= 79°.*** on all available times of light minimum,their orbital period changes are studied by using the O-C *** O-C diagram of LX Lyn reveals a cyclic oscillation with a period of about 14.84 yr and an amplitude of 0.0019 days,which can be explained by the light-travel time effect(LTTE) due to the presence of a third body with a minimum mass of0.06M_⊙.for V0853 Aur,it is discovered that the O-C diagram of the system also shows a cyclic oscillation with a period of 9.64 yr and an amplitude of 0.03365 *** cyclic oscillation of V0853 Aur can be attributed to the LTTE by means of a third body with a mass no less than 3.77M_⊙.The third body may play an important role in the formation and evolution of these systems.

关键词： stars:activity (stars:)binaries(including multiple):close (stars:)binaries:eclipsing (stars:)brown dwarfs stars:evolution stars:formation

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Quantitative assessment of the breast cancer marker HEr2 using a gold nanoparticle-based lateral flow immunoassay

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Nano research 2024年第6期17卷 5452-5460页

作者： Liya Ye Xinxin xu Aihua Qu Liqiang Liu Chuanlai xu Hua Kuang International Joint research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Human epidermal growth factor receptor 2(HEr2)is an important biomarker for detection and treatment of breast *** this study,we developed monoclonal antibodies against the extracellular domain(ECD)of HEr2 and established a rapid and accurate lateral flow immunoassay(LfIA)for use in community medical *** gene sequence of human HEr2-ECD was obtained from the National Center for Biotechnology Information(NCBI)to construct the expression ***2-ECD protein expressed in HEK293f cells was used to immunize BALB/c *** monoclonal antibodies were produced in mouse ascites and isolated by hybridoma cell *** were analyzed for purity by SDS-PAGE(sodium dodecyl sulphate-polyacrylamide gel-electrophoresis)and affinity was assessed by enzyme-linked immunosorbent assay(ELISA)while subtypes were detected using the commercial *** HEr2-ECD test strip was prepared based on the sandwich method and evaluated using a portable detection *** affinity of the paired antibodies,4D8 and 8D9,both reached 1×108 L/*** antibodies specifically recognized the HEr2-ECD protein in *** limit of detection(LOD)of the gold nanoparticle(AuNP)-based LfIA was 1.7 ng/mL with a detection range of 1.7-400 ng/mL,and the performance of the HEr2-ECD strip correlated well with that of a Siemens chemiluminescent immunoassay(CLIA)*** conclusion,the paired antibodies were successfully prepared with high affinity and *** AuNP-based LfIA of HEr2-ECD provides a fast and accurate method to detect the concentration of HEr2-ECD in serum samples for clinical use in community medical institutions,and could contribute to determining the progress of the disease or the effectiveness of treatment.

关键词： human epidermal growth factor receptor 2 breast cancer monoclonal antibody quantitative lateral flow immunoassay