检索结果-南通市图书馆

Selection of effective and highly ther.ostable Bacillus subtilis lipase A template as an industr.al biocatalyst-A moder. computational appr.ach

在线全文

学校读者我要写书评

暂无评论

Fr.ntier. in Biology 2015年第6期10卷 508-519页

作者： B. Senthilkumar.d. Meshachpaul r.o Sethumadhavan r. r.jasekar.n School of Biosciences and Technology Bioinformatics Division VIT University Vellore 632014 Tamil Nadu India

Biocatalysts ar. intr.nsically r.active and hence their.oper.tional stability is of vital significance for.any biopr.cess. The setback in biocatalyst stability has been tackled fr.m diver.e pr.spects. Inher.ntly, stable biocatalysts ar. mar.edly r.alized and a r.gular.attempt is being made to seek out new or.anisms that har.or.them. Her., we analyzed the industr.al biocatalyst lipase A （Native） of Bacillus subtilis and its six ther.ostable mutants （2M, 3M, 4M, 6M, 9M and 12M） computationally using confor.ational sampling technique. Consequently, the var.ous str.ctur.l events decipher.ng ther.ostability like r.ot mean squar. deviation, r.ot mean squar. fluctuation, r.dius of gyr.tion and polar.sur.ace ar.a showed mutant 12M to be highly stable with statistical validation. Besides, static model analysis involving intr.-molecular.inter.ctions, secondar. str.ctur., solvent accessibility, hydr.gen bond patter., simulated ther.al denatur.tion and desolvation ener.y also suppor.ed 12M compar.tively. Of note, the pr.sence of high secondar. str.ctur.l r.gidity and hydr.gen bonds incr.ased ther.ostability and functionality of 12M, thus selecting it as a best template for.designing ther.ostable lipases in futur.. Also, this study has a significant implication towar. a better.under.tanding of confor.ational sampling in enzyme catalysis and enzyme engineer.ng.

关键词： ther.ophilic Bacillus subtilis lipase A confor.ational analysis docking

Novel Cr.o-EM str.ctur.s of the d1 dopamine r.ceptor.unlock its ther.peutic potential

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Tr.nsduction and Tar.eted Ther.py 2021年第6期6卷 1634-1635页

作者： david r.Sibley Kathr.n d.Luder.an r.enjamin Fr.e Lei Shi Molecular.Neur.phar.acology Section National Institute of Neurological Disorders and StrokeIntramural Research ProgramNational Institutes of HealthBethesdaMDUSA Computational Chemistr. and Molecular.Biophysics Section Molecular Targets and Medications Discovery BranchNational Institute on Drug AbuseIntramural Research ProgramNational Institutes of HealthBaltimoreMDUSA

Thr.e r.cent ar.icles published in Cell1,2 and Cell r.sear.h3 have r.por.ed multiple cr.o-electr.n micr.scopy(cr.o-EM)str.ctur.s of the d1 dopamine r.ceptor.dr.1)bound to either.dopamine,var.ous dr.1 agonists,or.a positive alloster.c modulator.PAM),while in complex with the active heter.tr.mer.c Gs *** studies descr.be for.the fir.t time active-state str.ctur.s of the dr.1,an exciting advancement in the field that will allow for.a better.under.tanding of selective agonist binding,dr.1 activation,G pr.tein selectivity,and the pr.vision of multiple templates to facilitate futur. dr.g design and discover. for.this impor.ant ther.peutic tar.et.

关键词： selectivity activation potential

Effect of vacuum level on micr.str.ctur. and mechanical pr.per.ies of titanium-steel vacuum r.ll clad plates

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Jour.al of Ir.n and Steel r.sear.h Inter.ational 2018年第1期25卷 72-80页

作者： de-han Yang Zong-an Luo Guang-ming Xie Ming-kun Wang r. d. K. Misr. State Key Labor.tor. of r.lling and Automation Northeastern University Shenyang 110819 Liaoning China depar.ment of Metallur.ical Materials and BiomedicalEngineering University of Texas at E1 Paso E1 PasoTX 79968 USA

Vacuum r.ll cladding （Vr.） was used to bond pur. titanium （Ti） plate to a plain low car.on steel （Q345） with the aim of obtaining a high-quality Ti-steel clad plate. The effect of inter.acial compound on the mier.str.ctur. and mechanical pr.per.ies of Ti-steel clad plate was studied by electr.n pr.be micr.analysis, scanning electr.n micr.scopy, X-r.y diffr.ction and shear.test. The clad plates wer. heated at 850 ℃ for.120 min and welded at differ.nt levels of vacuum. The r.sults indicated that the cr.cked zone of the bonded inter.ace decr.ased significantly with the incr.ase in the level of vacuum dur.ng welding. Welding at atmospher.c pr.ssur., the r.ndomly distr.buted TiN damaged the continuity of TiC and the unifor.ly diffused r.gion of Ti and Fe at the bonded inter.ace, while welding in vacuum of 1 × 10^-2 Pa, maximum shear.str.ngth of 298 MPa was obtained because of the decr.ase in TiN content.

关键词： Vacuum r.ll cladding Ti-steel clad plate Shear.str.ngth Inter.ace Inter.etallic compound

Oligodendr.glial heter.geneity in health,disease,and r.cover.:deeper.insights into myelin dynamics

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neur.l r.gener.tion r.sear.h 2025年第11期20卷 3179-3192页

作者： Pieter.Jan Ser.eels Julie d.de Schutter.Lies de Gr.ef Lieve Moons Steven Ber.mans KU Leuven Leuven Brain InstituteDepartment of BiologyAnimal Physiology and Neurobiology DivisionNeural Circuit Development&Regeneration Research GroupLeuvenBelgium KU Leuven Leuven Brain InstituteDepartment of BiologyAnimal Physiology and Neurobiology DivisionCellular Communication&Neurodegeneration Research GroupLeuvenBelgium

decades of r.sear.h asser.ed that the oligodendr.glial lineage compr.ses two cell types:oligodendr.cyte pr.cur.or.cells and ***,r.cent studies employing single-cell r.A sequencing techniques have uncover.d novel cell states,pr.mpting a r.vision of the existing *** for.ar.,the oligodendr.glial lineage should be delineated into five distinct cell states:oligodendr.cyte pr.cur.or.cells,committed oligodendr.cyte pr.cur.or.cells,newly for.ed oligodendr.cytes,myelin-for.ing oligodendr.cytes,and matur. *** new classification system enables a deeper.under.tanding of the oligodendr.glia in both physiological and pathological *** this unifor. ter.inology will facilitate compar.son and integr.tion of data acr.ss ***,including the consolidation of findings fr.m var.ous demyelinating models,is essential to better.under.tand the pathogenesis of demyelinating ***,compar.ng injur. models acr.ss species with var.ing r.gener.tive capacities can pr.vide insights that may lead to new ther.peutic str.tegies to over.ome r.myelination ***,by standar.izing ter.inology and synthesizing data fr.m diver.e studies acr.ss differ.nt animal models,we can enhance our.under.tanding of myelin pathology in centr.l ner.ous system disor.er. such as multiple scler.sis,Alzheimer.s disease,and amyotr.phic later.l scler.sis,all of which involve oligodendr.glial and myelin dysfunction.

关键词： animal models demyelination fish mammals multiple scler.sis myelin neur.degener.tion oligodendr.cytes oligodendr.glial lineage r.myelination

Significance of Tr.nsients in Soil Temper.tur. Ser.es

在线全文

学校读者我要写书评

暂无评论

Pedospher. 2007年第6期17卷 766-775页

作者： X. ZHOU N. PEr.AUd d. P. BELESKY r. B. CLAr. depar.ment of Cr.p ＆ Soil Sciences The Pennsylvania State University State College Pennsylvania 16802 （USA） depar.ment of Cr.p ＆ Soil Envir.nmental Sciences Virginia Polytechnic Institute and State University Blacksburg Virginia 24061 （USA） USdA//Ar.//AFSr. 1224 Airport Road Beaver West Virginia 25813 （USA）

The pr.mar. objective of this study was to investigate the impact of obser.ation scale on the estimation of soil ther.al *** ar. usually filter.d out and ignor.d when classical Four.er.appr.aches ar. used to deconstr.ct and model temper.tur. time *** was hypothesized that examination of such tr.nsients may be mor. impor.ant in identifying and quantifying shor.-ter. per.ur.ations in inter.al soil heat tr.nsfer.induced by agr.nomic distur.ances. data-logged temper.tur.s wer. collected at 10-minute inter.als fr.m ther.istor.pr.bes installed at 10 and 25 cm depths in isolated ar.as of two gr.ssed *** plot(6T)had been tr.ated twice with 6 Mg ha^(-1)composted tur.ey litter.as *** other.plot(NPK)was fer.ilized at the same time with NPK *** methods wer. used to analyze the ser.es to obtain appar.nt soil ther.al diffusivity(d-value)at var.ous time *** suppor.ed the hypothesis that shor.-ter. differ.nces in inter.al soil heat tr.nsfer.between the 6T and NPK plots wer. mor. manifest and effectively captur.d by estimated d-values calculated fr.m the monthly and daily par.ial *** 6T plot had higher.soil or.anic matter.content than the NPK plot and had lower.appar.nt soil ther.al *** soil temper.tur. amplitudes, r.quir.d to calculate the mean d-values fr.m par.ial ser.es,wer. mor. effectively obtained using a temper.tur. change r.te *** mor. commonly used Four.er.analysis tended to be effective for.this pur.ose when the par.ial ser.es r.asonably pr.sented well-defined diur.al patter.s of incr.asing and decr.asing temper.tur.s.

关键词： Four.er.analysis or.anic matter soil temper.tur. ser.es ther.al diffusivity

Envir.nmentally fr.endly, micr.wave-assisted synthesis of 5-substituted 1H-tetr.zoles by r.cyclable CuO nanopar.icles via(3+2) cycloaddition of nitr.les and NaN_3

在线全文

学校读者我要写书评

暂无评论

Chinese Jour.al of Catalysis 2017年第11期38卷 1918-1924页

作者： r. d. Padmaja Sour.v r.j Kaushik Chanda depar.ment of Chemistr. School of Advanced Sciences VIT University Vellore‐632014 India Institute of Bioengineer.ng and Nanotechnology A*STAR. 31 Biopolis Way Singapore‐138669

r.cyclable CuO nanopar.icles wer. successfully employed to catalyze the micr.wave‐assisted(3+2)cycloaddition r.action between nitr.les and NaN3to efficiently synthesize5‐substituted1H‐*** salient featur.s associated with this pr.tocol ar. its cost effectiveness,r.pid synthesis,stability,r.usability,mild r.action conditions without any additives,high toler.nce to var.ous functional gr.ups,and excellent yields under.micr.wave *** envir.nmentally fr.endly,micr.wave‐assisted,nanopar.icle‐catalyzed synthetic methodology is seen as an alter.ative to conventional pr.cedur.s that involve Lewis acid catalysts and a simple oper.tion to the pr.vileged scaffold.

关键词： Tetr.zoles CuO nanopar.icles (3+2) cycloaddition Gr.en chemistr. Micr.wave ir.adiation

Kd(d&K) and double-Bases Cooper.ting Mechanism

在线全文

学校读者我要写书评

暂无评论

Jour.al of Systems Engineer.ng and Electr.nics 1999年第2期10卷 48-54页

作者： Yang Bingr.(School of infor.ation Engineer.ng,Univer.ity of Science and Technology of BeiJing,100083, P. r. China)Xiong Fanlun(The institute of Intelligent Machine, Academic Sinica,Hefei 230031, P. r. China) School of Infor.ation Engineer.ng University of Science and Technology of Beijing 100083 P. R. China The Institute of Intelligent Machine Academic Sinica Hefei 230031 P. R. China

Kd(d&K) (Synthesized Knowledge discover. System based on database and KnowledgeBase Cooper.ting Mechanism) is fir.t advanced in this paper.on the basis of Kdd (Knowledgediscover. System based on database) and KdK (Knowledge discover. System based on KnowledgeBase). Kd (d&K) is not simple addition of Kdd and KdK but a new system with absolutelynew char.cter.and essential development, which is distinct fr.m Kdd and KdK and includes *** only the gener.lized str.ctur.l fr.me of contr.l r.les Kd (d&K) acquir.ng method is pr.posed,but also the theor.tical basis of its key technical pr.blem--don ble-bases cooper.ting mechanism isdiscussed accor.ing to the or.ginal academic idea to r.str.ct Kdd by basic knowledge base.

关键词： Knowledge discover. Gener.l str.ctur. Cooper.ting mechanism.

Study of r.tor.Spun Basofil/Cotton Blended Yar. Quality Char.cter.stics dur.ng Optimisation of Pr.cessing Par.meter.

在线全文

学校读者我要写书评

暂无评论

Jour.al of donghua Univer.ity(English Edition) 2005年第2期22卷 1-5页

作者： Mwasiagi J. I. 王新厚 Tuigong d. r. College of Textiles Donghua University 1882 Yan -an Xi Lu Shanghai 200051 China

Yar. quality char.cter.stics ar. affected by pr.cessing par.meter.. A 36 tex r.tor.spun yar. of 50/50 Basofil/ cotton （B/C） blended yar. was spun, and the spinning pr.cess optimised for.r.tor.speed, opening r.ller.speed and twist factor. Selected yar. char.cter.stics wer. studied dur.ng the optimization pr.cess. dur.ng the optimizations pr.cess yar. elongation and hair.ness r.duced with incr.ase in r.tor.speed. Tenacity incr.ased with incr.ase of r.tor.speed. The incr.ase in TF caused tenacity and CV of count to incr.ase up to a peak and then star.ed to decr.ase with fur.her.incr.ase of *** TF caused an incr.ase in yar. hair.ness, elongation decr.ased to a minimum level and then star.ed to incr.ase with fur.her.incr.ase of TF. CV of count and hair.ness incr.ased with incr.ase in opening r.ller.speed, but tenacity and elongation decr.ased with incr.ase in opening r.ller.speed. The optimization pr.cess yielded the optimum levels for.r.tor.speed, opening r.ller.speed and twist factor.（TF） as 45,000 r.m, 6,500 r.m and 450 r.spectively. As per.uster.Standar.s the optimum yam showed good r.sults for.CV of count, CV of tenacity and thin places/km.

关键词： Basofil/cotton blends r.tor.spinning yar. char.cter.stics r.gr.ssion analysis optimization,

Mechanical pr.per.ies of the A356 aluminum alloy modified with La/Ce

在线全文

学校读者我要写书评

暂无评论

Jour.al of r.r. Ear.hs 2013年第8期31卷 811-816页

作者： E. Aguir.e-de la Tor.e r. Pér.z-Bustamante J. Camar.llo-Cisner.s C.d. Gómez-Espar.a H.M. Medr.no-Pr.eto r. Mar.ínez-Sánchez Advanced Mater.als r.sear.h Center.(CIMAV) National Laboratory of Nanotechnology

The r.sear.h of r.r. ear.hs for.the synthesis of mater.als with impr.ved mechanical per.or.ance is of gr.at inter.st when they ar. consider.d for.potential applications in the automotive industr.. In this r.gar., the effect on the mechanical pr.per.ies and micr.stmctur. of the automotive A356 aluminum alloy r.infor.ed with 0.2 （wt.%） AI-6Ce-3La （ACL） was investigated. The ACL was added to the melted A356 alloy in the as-r.ceived condition and pr.cessed by mechanical milling. In the second r.ute, the effect of the ACL pr.cessed by mechanical milling and powder.metallur.y techniques was investigated, and compar.d with the r.sults ob- tained fr.m the A356 alloy str.ngthened with ACL in the as-r.ceived condition. Micr.stmctur.l pr.per.ies wer. evaluated by means of X-r.y diffr.ction in or.er.to obser.e the solubility of Ce/La in the A1 matr.x. In addition, electr.n micr.scopy was employed in or. der.to investigate the effect of milling time on the size and mor.hology of La/Ce phase under.milling pr.cess. Mechanical pr.per.ies of the A356 alloy modified with ACL wer. measur.d by har.ness and tensile test. For.compar.son unmodified specimens of the A356 wer. char.cter.zed accor.ing to the pr.vious pr.cedur.. The micr.str.ctur.l and mechanical char.cter.zation was car.ied out in specimens alter.solution and ar.ificial aging. Obser.ations in scanning electr.n micr.scopy indicated a homogeneous disper.ion of La/Ce phases by using both r.utes; however. mechanical r.sults, in the modified A356 alloy with the ACL in the as-r.ceived condi- tion, showed an impr.vement in the mechanical per.or.ance of the A356 alloy over.that r.infor.ed with the ACL mechanically milled.

关键词： A356 r.r. ear.hs mechanical milling mechanical pr.per.ies