检索结果-南通市图书馆

长春花、柑橘离体再生和农杆菌介导的遗传转化研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

长春花、柑橘离体再生和农杆菌介导的遗传转化研究

作者： qurban ali 华中农业大学

学位级别：硕士

长春花(Catharnthus roseus)属于夹竹桃科,是一种在热带地区发现的多年生植物,又被称为vinca roseus或者Madagascar periwinkle。长春花也是黄龙病菌亚洲种(CLas)的宿主。CLas在长春花上表现出不同的症状。与柑橘相比,柑橘黄龙病在长春... 详细信息

长春花(Catharnthus roseus)属于夹竹桃科,是一种在热带地区发现的多年生植物,又被称为vinca roseus或者Madagascar periwinkle。长春花也是黄龙病菌亚洲种(CLas)的宿主。CLas在长春花上表现出不同的症状。与柑橘相比,柑橘黄龙病在长春花上能在较短的时间内表现出典型症状。因此长春花是研究CLas致病机理和病原寄主互作的理想试验材料。在本研究中,我们以下胚轴、叶片和枝条为外植体,优化了植物生长激素对长春花愈伤组织诱导和再生的影响。同时,首次将红色荧光蛋白(Ds Red2)作为长春花遗传转化的分子标记,成功建立了长春花遗传转化技术。主要研究成果如下:本研究对不同浓度的6-苄基氨基嘌呤(BAP)、萘乙酸(NAA)和2,4-二氯苯氧乙酸(2,4-D)、吲哚丁酸(IBA)对长春花遗传再生的影响进行了优化,结果表明,长春花下胚轴在M6培养基:MS+BAP(2.0 mg/L)+NAA(1.0 mg/L)+2,4-D(1.0 mg/L)上,愈伤组织诱导率最高为98.8%。叶片外植体在M5培养基:MS+BAP(2.0 mg/L)+NAA(0.5 mg/L)+2,4-D(0.5 mg/L)上愈伤组织诱导率最高为94.0%。此外,直接芽再生试验表明:在MS1培养基:MS+6BA(1.0 mg/L)+NAA(0.4 mg/L)上,平均每个单芽产生7.0±2.0个新梢,芽诱导率最高为97.0%。而在通过诱导愈伤组织间接芽再生的器官发生途径中,在MS1[MS+BAP(1.0 mg/L)+NAA(0.4 mg/L)]和MS5[MS+BAP(2.0 mg/L)+NAA(0.3 mg/L)]培养基中,芽诱导率最高均为94.0%。在MS1培养基:MS+BAP(1.0 mg/L)+NAA(0.4 mg/L)内芽增值率最高,平均为18.7±3.51。在MS5培养基:[MS+BAP(2.0 mg/L)+NAA(0.3 mg/L)]芽增值率最低,平均为15.7±5.13。在[1/2MS+IBA 2.5 mg/L+NAA(0.5 mg/L)+蔗糖(30 g/L)+琼脂(5.5 g/L)]培养基中的生根率最高为88.0%。此外,利用农杆菌EHA105和pCAMBIA2300::35S::DsRed2介导的二元载体包含增强的35S启动子,长春花变种Pacific white(PW),Pacific Grapevine Red(PGR)和Pacific cherry Red(PCR)的遗传转化技术)已优化。最佳转化条件是:以长春花下胚轴为外植体,根癌农杆菌侵染25分钟,19°C条件下在1/2MS+AS(100μM)培养基上共培养2天,效果最佳。通过对不同浓度的卡那霉素(40,50,70 mg/L)对再生效率的影响的优化,长春花PGR、PCR和PW的遗传再生率分别为55.5%、45.5%和38.0%。通过对Ds Red2蛋白表达的肉眼观察和荧光显微镜下镜检观察,Ds Red2不仅在愈伤组织中可以检测到,而且在叶、茎等器官中都有很强的信号。进一步结合PCR方法对转基因系中的Ds Red2的表达进行验证,在获得的30个不同品种的转基因系中,阳性植株为21株,PGR、PCR和PW的阳性转化率分别为77.7%、75.0%和55.5%。运用含有Ca MV-35S启动子的双元载体Ds Red2,对两个柑橘品种甜橙(Citrus sinensis)和沙田柚(Citrus maxima)进行了遗传转化研究。分别以柑橘上胚轴为外植体,通过对遗传转化参数的的优化,获得最佳遗传转化方法为:根癌农杆菌最适浓度OD600=0.6,最佳侵染时间为25 min,在19°C条件下,在MT+麦芽提取物(0.5 g/L)+L-谷氨酰胺(1.5 g/L)培养基中共培养72 h,转化效率最高。甜橙最高转化率为50.0%,沙田柚的最高转化效率为35.5%。利用荧光显微镜观察到Ds Red2蛋白在甜橙和沙田柚的叶、茎等部位都产生较强的红色信号。总之,本试验首次成功的将Ds Red2荧光蛋白作为标记,运用于长春花和柑橘遗传转化,成功获得了转基因阳性植株,为下一步开展利用基因编辑技术进行抗黄龙病病育种工作奠定了基础。

关键词： DsRed2 Agrobacterium tumefaciens Catharanthus roseus citrus genetic transformation Candidatus Liberibacter asiaticus

Spatial Trend of Foot and Mouth Disease Virus (FMDV) Serotypes in Cattle and Buffaloes, Pakistan

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

Virologica Sinica 2012年第5期27卷 320-323页

作者： Muhammad Abubakar Muhammad Javed Arshed qurban ali Manzoor Hussain National Veterinary Laboratory

The present study describes the frequency of Foot and Mouth Disease （FMD） virus serotypes （O, A and Asia-l） in major regions （all provinces） of Pakistan using Indirect Sandwich ELISA. Also, spatial distribution of various FMD serotypes and their comparison is discussed. A total of 590 samples （Epithelial tissue） have been analyzed during a period of five years （2005-2009）. Out of 590 samples, 180 were found positive, giving an overall confirmation of FMDV about 33.2 %. Of the prevalent serotypes, FMDV ＇O＇ serotype caused most outbreaks （20.7 %）, followed by serotype A （6.6 %） and serotype Asia-1 （4.6 %） while there was no positive case of type ＇C＇. The study clearly showed that the disease was more frequent in the agro-climatic zones than in hilly areas. Based on the data of 590 samples （〉50 outbreaks）, the overall prevalence of FMDV in cattle and buffaloes in Pakistan was 33.2 %, while in cattle alone, it was 37.1%, higher than in buffalo （28.7 %）. There were eight cases of mixed serotypes infection, indicating the presence of endemic state of disease. Another significant feature was the change over time. In phase-I （2005-2007）, there was an overall prevalence of 29.4 %, while the occurrence of the serotype O, A and Asia-1 was 20.4 %, 2.9 % and 4.7 %, respectively. During phase-II （2008-2009）, the overall prevalence was 59.21%, while those of serotype O, A and Asia-1 were 22.4 %, 31.6 % and 4.0 %, respectively. This clearly indicated a shift from serotype O to A, which may help to explain the occurrence of more severe outbreaks, despite vaccination.

关键词： Spatial and Temporal Distribution Serotype shift Foot and Mouth disease (FMD) Virus Indirect Sandwich ELISA Serotypes

Effects of December 2014 Great Flood on the Physico-Chemical Properties of the Soils in the Kelantan Plains, Malaysia

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Water Resource and Protection 2016年第2期8卷 263-276页

作者： Jusop Shamshuddin qurban ali Panhwar Radziah Othman Roslan Ismail Hamdan Jol Melissa alina Yusoff Department of Land Management Faculty of Agriculture Universiti Putra Malaysia Serdang Malaysia Soil Science Division Nuclear Institute of Agriculture Tandojam Pakistan

Heavy rain falling on land at the upper reaches of the Kelantan River, Malaysia, on December 2014, had resulted in severe soil erosion and untold damages to croplands. The lower reaches of the river were heavily silted with infertile materials considered unfit for crop production. A study was conducted to explain why the flood phenomenon occurred, to determine the physico-chemical properties of the sediments silted in the Kelantan Plains and to propose measures for soil mitigation. Results showed that the silted sediments were characterized by the presence of quarts, mica, feldspars, kaolinite, gibbsite and hematite believed to come from the top- and subsoil of the upland areas. The sediments’ pH was very low and Al and/or Fe contents were very high, while nitrogen and carbon contents varied from area to area. Soils in the Kelantan Plains badly affected by this great flood needed to undergo proper ameliorative program. The most appropriate measure would be to apply ground magnesium limestone in combination with bio-fertilizer fortified with beneficial microbes that would increase their pH to a level above 5, which consequently eliminates Al3+ and/or Fe2+ that causes toxicity to the crops growing on them. The organic material so added would enhance the formation of soil structures. It is advised that the farming communities in the upper reaches of the Kelantan River would have to follow the advice advocated by the Department of Agriculture, Peninsular Malaysia, via MyGAP initiative, in order to sustain agricultural production on their land.

关键词： Crop Production Great Flood Heavy Rainfall Silted Sediments Soil Properties

A near-complete genome assembly of Catharanthus roseus and insights into its vinblastine biosynthesis and high susceptibility to the Huanglongbing pathogen

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plant Communications 2023年第6期4卷 216-220页

作者： Zhongping Xu Guanying Wang Qiongqiong Wang Xiaoting Li Guangyu Zhang ali qurban Can Zhang Yi Zhou Huan Si Lisong Hu Fuqiu Wang Ying Wang Zhitao Tian Wei Chen Shuangxia Jin Fang Ding Hubei Key Laboratory of Plant Pathology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubeiChina National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhanHubeiChina Tobacco Research Institute Chinese Academy of Agricultural SciencesQingdaoShandongChina Spice and Beverage Research Institute Chinese Academy of Tropical Agricultural SciencesWanningHainanChina National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhanHubeiChina Key Laboratory of Horticultural Plant Biology of MOE(Ministry of Education) Huazhong Agricultural UniversityWuhanHubeiChina

Dear Editor,Catharanthus roseus,an important medicinal plant native to Madagascar,is the sole source of the two important anticancer drugs vinblastine and vincristine,which are synthesized by the monoterpene-derived indole alkaloid(MIA)pathway(Das et al.,2020).It is also an important model plant for the study of Huanglongbing(HLB),a devastating citrus disease that causes severe economic losses worldwide(Ma et al.,2022).Vincristine is an FDA-approved drug that has been used for acute lymphocytic leukemia,lymphoid blast crisis of chronic myeloid leukemia,and Hodgkin’s/non-Hodgkin’s lymphoma(Below and M Das,2021).

关键词： drugs lymphocytic

亚金跳小蜂在不同生育期扶桑绵粉蚧寄主上的生物学特性（英文）

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

南方农业学报 2019年第9期50卷 1973-1980页

作者： qurban ali Rind Abdul Ghani Lanjar Abdul Ghafoor Rahoo Ghulam Sarwar Solangi 吕荣华付岗覃武巴基斯坦信德农业大学昆虫学系巴基斯坦乌梅尔蔻特信德70060 巴基斯坦信德农业大学昆虫学系巴基斯坦坦杜贾姆信德69100 广西农业科学院国际合作处南宁530007 广西农业科学院植物保护研究所南宁530007

【目的】探讨亚金跳小蜂对不同生育期扶桑绵粉蚧的影响,为今后寄生蜂的饲养和推广及扶桑绵粉蚧的生物防治提供理论参考。【方法】分别以4个龄期的15头扶桑绵粉蚧、30头3龄幼虫和成虫为试虫进行选择性和非选择性试验,每虫笼放入1对亚金... 详细信息

【目的】探讨亚金跳小蜂对不同生育期扶桑绵粉蚧的影响,为今后寄生蜂的饲养和推广及扶桑绵粉蚧的生物防治提供理论参考。【方法】分别以4个龄期的15头扶桑绵粉蚧、30头3龄幼虫和成虫为试虫进行选择性和非选择性试验,每虫笼放入1对亚金跳小蜂,每天记录寄主和寄生蜂的存活情况。【结果】亚金跳小蜂的最大寄生率和扶桑绵粉蚧的最大僵虫量均在扶桑绵粉蚧3龄若虫期出现,而最低值均在其2龄若虫期出现。在选择和非选择试验中,亚金跳小蜂雌虫的寿命差异不显著(P>0.05)。在以扶桑绵粉蚧2龄若虫为试验对象的选择性和非选择性试验中,雌性亚金跳小蜂的生命周期分别为12.69±0.19和13.24±0.40d。在以扶桑绵粉蚧3龄若虫为试验对象的选择性和非选择性试验中,雌性亚金跳小蜂的生命周期分别为15.51±0.28和15.12±0.14d。寄生蜂的发育周期因寄主龄期而有明显差异,在扶桑绵粉蚧3龄若虫期寄生的亚金跳小蜂生命周期最长,而在扶桑绵粉蚧3龄若虫期寄生的亚金跳小蜂生命周期最短。【结论】作为寄生蜂的亚金跳小蜂在寄主扶桑绵粉蚧3龄若虫期的成虫量最大,在2龄若虫期的成虫量最小。

关键词：亚金跳小蜂扶桑绵粉蚧生育期寄主生物学特性

A Support Vector Machine(SVM)Model for Privacy Recommending Data Processing Model(PRDPM)in Internet of Vehicles

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2025年第1期82卷 389-406页

作者： ali Alqarni Department of Computer Science and Artificial Intelligence College of Computing and Information TechnologyUniversity of BishaBisha61922Saudi Arabia

Open networks and heterogeneous services in the Internet of Vehicles(IoV)can lead to security and privacy *** key requirement for such systems is the preservation of user privacy,ensuring a seamless experience in driving,navigation,and *** privacy needs are influenced by various factors,such as data collected at different intervals,trip durations,and user *** address this,the paper proposes a Support Vector Machine(SVM)model designed to process large amounts of aggregated data and recommend privacy preserving *** model analyzes data based on user demands and interactions with service providers or neighboring *** aims to minimize privacy risks while ensuring service continuity and *** SVMmodel helps validate the system’s reliability by creating a hyperplane that distinguishes between maximum and minimum privacy *** results demonstrate the effectiveness of the proposed SVM model in enhancing both privacy and service performance.

关键词： Support vector machine big data IoV privacy-preserving

MARIE:One-Stage Object Detection Mechanism for Real-Time Identifying of Firearms

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Computer Modeling in Engineering & Sciences 2025年第1期142卷 279-298页

作者： Diana Abi-Nader Hassan Harb ali Jaber ali Mansour Christophe Osswald Nour Mostafa Chamseddine Zaki Computer Science Department Faculty of SciencesLebanese UniversityBeirut146404Lebanon College of Engineering and Technology American University of the Middle EastEgaila54200Kuwait Lab-STICC CNRS UMR 6285ENSTA-BretagneBrest29200France

Security and safety remain paramount concerns for both governments and individuals *** today’s context,the frequency of crimes and terrorist attacks is alarmingly increasing,becoming increasingly intolerable to ***,there is a pressing need for swift identification of potential threats to preemptively alert law enforcement and security forces,thereby preventing potential attacks or violent *** advancements in big data analytics and deep learning have significantly enhanced the capabilities of computer vision in object detection,particularly in identifying *** paper introduces a novel automatic firearm detection surveillance system,utilizing a one-stage detection approach named MARIE(Mechanism for Realtime Identification of Firearms).MARIE incorporates the Single Shot Multibox Detector(SSD)model,which has been specifically optimized to balance the speed-accuracy trade-off critical in firearm detection *** SSD model was further refined by integrating MobileNetV2 and InceptionV2 architectures for superior feature extraction *** experimental results demonstrate that this modified SSD configuration provides highly satisfactory performance,surpassing existing methods trained on the same dataset in terms of the critical speedaccuracy *** these innovations,MARIE sets a new standard in surveillance technology,offering a robust solution to enhance public safety effectively.

关键词： Firearm and gun detection single shot multi-box detector deep learning one-stage detector MobileNet inception convolutional neural network

Vagus nerve stimulation in intracerebral hemorrhage:the need for further research

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第11期20卷 3213-3214页

作者： Sheharyar S.Baig ali N.ali Arshad Majid Sheffield Institute for Translational Neuroscience Division of NeuroscienceThe University of SheffieldSheffieldUK

Vagus nerve stimulation(VNS)and stroke:Stroke is the second leading cause of death and the third leading cause of disability worldwide(Baig et al.,2023).There have been significant paradigm shifts in the management of acute ischemic stroke through mechanical *** chronic ischemic stroke,invasive VNS paired with rehabilitation is associated with a significant increase in upper limb motor recovery and is FDA-approved(Baig et al.,2023).There are no treatments of similar efficacy in acute intracerebral hemorrhage(ICH)where several promising trials,e.g.,TICH-2,STOP-AUST,and TRAIGE did not show improvements in functional outcomes(Puy et al.,2023).

关键词： stimulation acute

Comprehensive Review and Analysis on Facial Emotion Recognition:Performance Insights into Deep and Traditional Learning with Current Updates and Challenges

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2025年第1期82卷 41-72页

作者： Amjad Rehman Muhammad Mujahid Alex Elyassih Bayan AlGhofaily Saeed ali Omer Bahaj Artificial Intelligence&Data Analytics Lab College of Computer&Information SciencesPrince Sultan UniversityRiyadh11586Saudi Arabia MIS Department College of Business Administration Prince Sattam Bin Abdulaziz UniversityAlKharj11942Saudi Arabia

In computer vision and artificial intelligence,automatic facial expression-based emotion identification of humans has become a popular research and industry *** demonstrations and applications in several fields,including computer games,smart homes,expression analysis,gesture recognition,surveillance films,depression therapy,patientmonitoring,anxiety,and others,have brought attention to its significant academic and commercial *** study emphasizes research that has only employed facial images for face expression recognition(FER),because facial expressions are a basic way that people communicate meaning to each *** immense achievement of deep learning has resulted in a growing use of its much architecture to enhance *** review is on machine learning,deep learning,and hybrid methods’use of preprocessing,augmentation techniques,and feature extraction for temporal properties of successive frames of *** following section gives a brief summary of assessment criteria that are accessible to the public and then compares them with benchmark results the most trustworthy way to assess FER-related research topics *** this review,a brief synopsis of the subject matter may be beneficial for novices in the field of FER as well as seasoned scholars seeking fruitful avenues for further *** information conveys fundamental knowledge and provides a comprehensive understanding of the most recent state-of-the-art research.

关键词： Face emotion recognition deep learning hybrid learning CK+ facial images machine learning technological development