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Agrobacterium–mediated Transformation of Kalanchoe laxiflora

Horticultural Plant Journal 2019年第5期5卷 221-228页

作者： Xiling Wang Xinlu Chen qunkang cheng Kaikai Zhu Xiaohan Yang Zongming cheng Department of Plant Sciences University of TennesseeKnoxvilleTN37996USA College of Biotechnology Southwest UniversityChongqing400715China Department of Entomology and Plant Pathology University of TennesseeKnoxvilleTN37996USA College of Horticulture Nanjing Agricultural UniversityNanjing210095China Biosciences Division Oak Ridge National LaboratoryOak RidgeTN37831-6422USA

Plants with crassulacean acid metabolism(CAM) generally utilize water 20%–80% more efficiently than non-CAM plants. The whole genomes of several CAM plants have been sequenced or are being sequenced. For effective genome characterization and genome editing of CAM plants,an efficient transformation system is essential. In this study, we developed an Agrobacterium-mediated transformation protocol for Kalanchoe laxiflora, an obligate CAM plant,by optimizing several factors affecting the transformation efficiency. Agrobacterium strains AGL1, C58, EHA105,and GV3101 were all suitable for K. laxiflora transformation. Fifty-nine percent of the leaf explants yielded kanamycin-resistant and GUS-positive shoots. Polymerase chain reaction and quantitative real-time PCR(qRT-PCR) using gus A-, gus Plus-, npt II-and hpt-specific primers confirmed that the transgenes were integrated into K. laxiflora genome and expressed. This efficient transformation system will allow effective functional characterization of genes through over-or down-expression, knockout, or genome editing.

关键词： Kalanchoe laxiflora Agrobacterium-mediated transformation CAM plants

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The grapevine kinome:annotation,classification and expression patterns in developmental processes and stress responses

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Horticulture Research 2018年第1期5卷 682-697页

作者： Kaikai Zhu Xiaolong Wang Jinyi Liu Jun Tang qunkang cheng Jin-Gui Chen Zong-Ming(Max)cheng College of Horticulture Nanjing Agricultural UniversityNanjingJiangsu210095China Department of Plant Sciences University of TennesseeKnoxvilleTN37996USA Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement Institute of HorticultureJiangsu Academy of Agricultural SciencesNanjingJiangsu210014China Department of Entomology and Plant Pathology University of TennesseeKnoxvilleTN37996USA Biosciences Division Oak Ridge National LaboratoryOak RidgeTN37831USA

Protein kinases(PKs)have evolved as the largest family of molecular switches that regulate protein activities associated with almost all essential cellular *** a fraction of plant PKs,however,have been functionally characterized even in model plant *** the present study,the entire grapevine kinome was identified and annotated using the most recent version of the grapevine genome.A total of 1168 PK-encoding genes were identified and classified into 20 groups and 121 families,with the RLK-Pelle group being the largest,with 872 *** 1168 kinase genes were unevenly distributed over all 19 chromosomes,and both tandem and segmental duplications contributed to the expansion of the grapevine kinome,especially of the RLK-Pelle ***/Ks values indicated that most of the tandem and segmental duplication events were under purifying *** grapevine kinome families exhibited different expression patterns during plant development and in response to various stress treatments,with many being *** comprehensive annotation of grapevine kinase genes,their patterns of expression and coexpression,and the related information facilitate a more complete understanding of the roles of various grapevine kinases in growth and development,responses to abiotic stress,and evolutionary history.

关键词： stress grape treatment

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GA_3与CPPU对大濑户葡萄无核化处理果实发育的影响

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中外葡萄与葡萄酒 2007年第5期 10-11,15页

作者：刘捷杨丽娜陶建敏许宽勇程群康刘艳红南京农业大学园艺学院南京210095

本文以大濑户葡萄品种为材料,在不同时期用不同浓度赤霉素(GA3)和吡效隆(CPPU)处理,研究对其果实发育的影响。结果表明,各处理都使大濑户葡萄的无核率大幅度提高。其中以花前1天GA37.5mg/L处理的无核率最高,达100%,而花前1天GA312.5mg/... 详细信息

本文以大濑户葡萄品种为材料,在不同时期用不同浓度赤霉素(GA3)和吡效隆(CPPU)处理,研究对其果实发育的影响。结果表明,各处理都使大濑户葡萄的无核率大幅度提高。其中以花前1天GA37.5mg/L处理的无核率最高,达100%,而花前1天GA312.5mg/L处理的无核率达93.8%,果实生长发育效果较好,单果最重达9.61g。

关键词：赤霉素吡效隆大濑户葡萄无核化

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