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A single-cell approach to engineer CD8+ T cells targeting cytomegalovirus

Cellular & Molecular Immunology 2021年第5期18卷 1326-1328页

作者： Fei Wang qumiao xu Zhenkun Zhuang Ziyi Li Qianqian Gao Yaling Huang Yonglun Luo Xiuqing Zhang Linnan Zhu Cheng-chi Chao BGI Education Center University of Chinese Academy of SciencesShenzhen518083China BGI-Shenzhen Shenzhen518083China Department of Biomedicine Aarhus University8000AarhusDenmark China National GeneBank BGI-ShenzhenShenzhen518120China Shenzhen Key Laboratory of Genomics Beishan Industrial ZoneShenzhen518083China School of Biology and Biological Engineering South China University of TechnologyGuangzhou510006China Northwest A&F University Yangling712100ShaanxiChina Guangdong Enterprise Key Laboratory of Human Disease Genomics Beishan Industrial ZoneShenzhen518083China AbVision IncMilpitasCAUSA

T lymphocytes are crucial for antiviral responses and provide a promising repertoire for potential therapies of viral diseases such as cytomegalovirus(CMV)infection1 and the ongoing COVID-19 pandemic caused by SARS-CoV-2.^(2) CMV-related diseases occur once the host immune system is impaired or lacks a protective repertoire of virus-specific T lymphocytes.3 Adoptive transfer of T-cell receptor(TCR)-engineered T cells(TCR-Ts)provides an encouraging alternative treatment option for patients with CMV reactivation.^(4) However,generating TCR-Ts requires the identification of epitope-specific and functional TCR *** single-cell sequencing techniques open up the ability to unravel TCR repertoires,^(5 )which offers a potential opportunity to screen functional TCR pairs for TCR-T ***,we report an efficient approach that combines ex vivo CD8+T-cell stimulation with single-cell RNA and TCR V(D)J sequencing to identify CMV-specific TCRs for generating TCR-Ts.

关键词： impaired protective stimulation

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Spatial transcriptome unveils a discontinuous inflammatory pattern in proficient mismatch repair colorectal adenocarcinoma

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Fundamental Research 2023年第4期3卷 640-646页

作者： Rongxin Zhang Yu Feng Wenjuan Ma Yanying Guo Mei Luo Young Li Yupeng Zang xuan Dong Shixun Lu Qiang Guo qumiao xu Huanyi Chen Yijian Li Longqi Liu Ao Chen Gong Chen xun xu Department of Colorectal Surgery Sun Yat-sen University Cancer CentreGuangzhou 510060GuangdongChina BGI-Shenzhen Beishan Industrial ZoneShenzhen 518083China State Key Laboratory of Oncology in South China Guangzhou 510060GuangdongChina Collaborative Innovation Center of Cancer Medicine Guangzhou 510060GuangdongChina Intensive Care Unit Department Sun Yat-sen University Cancer CentreGuangzhou 510060GuangdongChina College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China Department of Pathology Sun Yat-sen University Cancer CentreGuangzhou 510060GuangdongChina Shenzhen Bay Laboratory Shenzhen 518000China Department of Biology University of CopenhagenCopenhagen DK-2200Denmark Guangdong Provincial Key Laboratory of Genome Read and Write Shenzhen 518120China

The preexistence of immune cells in the tumor microenvironment substantiates the efficacy of immunotherapy in cancer *** the complex intratumoral immune heterogeneity has been extensively studied in single cell resolution,hi-res spatial investigations are *** this study,we performed a spatial transcriptome analysis of 4 colorectal adenocarcinoma specimens and 2 paired distant normal specimens to identify the molecular pattern involved in a discontinuous inflammatory response in pathologically annotated cancer *** on the location of spatially varied gene expression,we unmasked the spatially-varied immune ecosystem and identified the locoregional“warmed-up”immune response in predefined“cold”tumor with substantial infiltration of immune ***“warmed-up”immune profile was found to be associated with the in-situ copy number variance and the tissue remodeling ***,“warmed-up”signature genes indicated improved overall survival in CRC patients obtained from TCGA database.

关键词： Colorectal adenocarcinoma Mismatch repair Inflammation Spatial transcriptomics Tumor microenvironment

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