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Transumbilical single-port laparoscopic cholecystectomy using traditional laparoscopic instruments:a report of thirty-six cases

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第10期12卷 862-866页

作者： Li-ping CAO Ri-sheng que Fan zhou Guo-ping DING De-xi JING Department of Surgery the Second Affliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Objective:To evaluate the feasibility and safety of the operation of transumbilical single-port laparoscopic cholecystectomy (TSPLC) by traditional laparoscopic instruments and summarize the initial ***:Sixty subjects with cholelithiasis were divided into two *** group (36 cases) underwent TSPLC and the control group (24 cases) underwent traditional three-port laparoscopic cholecystectomy (LC).Postoperative complications were observed and operation time,hospital days,visual analogue scale (VAS) after 6 and 24 h of operation,and subject satisfaction score were ***:TSPLC and traditional LC were performed successfully in the two *** operation time in the TSPLC group was significantly longer than that in the control *** was no statistically significant difference in hospital stay and VAS between the TSPLC and control *** subject satisfaction score in the TSPLC group was 91.2,significantly higher than that in the control group (P<0.01).All subjects recovered from the operation and no postoperative complication occurred during the period of two weeks after ***:TSPLC is a feasible and safe method for cholecystectomy,although it may be more ***,it is welcomed by patients who are more concerned with cosmetic *** studies are needed to confirm its disadvantages and contraindications.

关键词： Laparoscopic cholecystectomy Single-incision laparoscopic cholecystectomy Minimally invasive surgery Traditional laparoscopic instruments

An improved deviation model for transonic stages in axial compressors

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Chinese Journal of Aeronautics 2024年第7期37卷 93-108页

作者： Xiaochen WANG Xuesong LI Xiaodong REN Chunwei GU Xiaobin que Guoyu zhou Department of Energy and Power Engineering Tsinghua UniversityBeijing 100084China China United Gas Turbine Technology Co.Ltd. Beijing 102209China

Deviation model is an important model for through-flow analysis in axial *** analysis in classical deviation models is developed under the assumption of onedimensional flow,which is controlled by the continuity *** consider three-dimensional characteristics in transonic flow,this study proposes an improved theoretical analysis method combining force analysis of the blade-to-blade flow with conventional analysis of the continuity *** of shock structures on transverse force,streamwise velocity and streamline curvature in the blade-to-blade flow are analyzed,and support the analytical modelling of density flow ratio between inlet and outlet ***,a novel deviation model for transonic stages in axial compressors is proposed in this *** empirical coefficients are corrected based on the experimental data of a linear cascade,and the prediction accuracy is validated with the experimental data of a three-stage transonic *** novel model provides accurate predictions for meridional flow fields at the design point and performance curves at design speed,and shows obvious improvements on classical models by Carter and C¸etin.

关键词： Axial compressor Transonic flow Deviation model Through-flow method Aerodynamic performance

Genome Size of Alexandrium catenella and Gracilariopsis lemaneiformis Estimated by Flow Cytometry

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Journal of Ocean University of China 2016年第4期15卷 704-710页

作者： DU Qingwei SUI Zhenghong CHANG Lianpeng WEI Huihui LIU Yuan MI Ping SHANG Erlei Zeeshan Niaz que zhou Key Laboratory of Marine Genetics and Breeding of Ministry of Education of China College of Marine Life Sciences Ocean University of China Department of Microbiology Hazara University MansehraKPK

Flow cytometry(FCM) technique has been widely applied to estimating the genome size of various higher plants. However, there is few report about its application in algae. In this study, an optimized procedure of FCM was exploited to estimate the genome size of two eukaryotic algae. For analyzing Alexandrium catenella, an important red tide species, the whole cell instead of isolated nucleus was studied, and chicken erythrocytes were used as an internal reference. The genome size of A. catenella was estimated to be 56.48 ± 4.14 Gb(1C), approximately nineteen times larger than that of human genome. For analyzing Gracilariopsis lemaneiformis, an important economical red alga, the purified nucleus was employed, and Arabidopsis thaliana and Chondrus crispus were used as internal references, respectively. The genome size of Gp. lemaneiformis was 97.35 ± 2.58 Mb(1C) and 112.73 ± 14.00 Mb(1C), respectively, depending on the different internal references. The results of this research will promote the related studies on the genomics and evolution of these two species.

关键词： Genome Arabidopsis eukaryotic nucleus genomics references cytometry algae purified chicken

Characteristics and influencing factors of CO_(2) emission from inland waters in China

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Science China Earth Sciences 2024年第6期67卷 2034-2055页

作者： Ting zhou Xiaofeng WANG Zuolin XIAO Zhaoyin QING Xianxiang LI Jilong WANG Ziyi que Chongqing Key Laboratory of Carbon Cycling and Carbon Regulation in Mountainous Ecosystems Chongqing 401331China School of Geography and Tourism Chongqing Normal UniversityChongqing 401331China Chongqing Field Observation and Research Station of Earth Surface Ecological Processes in Three Gorges Reservoir Area Chongqing 405400China School of Earth System Science Tianjin UniversityTianjin 300072China

Inland water bodies,being the most active biogeochemical cycle reactors,play a pivotal role in the global carbon cycle and CO_(2) *** study integrates existing observation dataset of CO_(2) flux(fCO_(2))in rivers,lakes and reservoirs in China,to elucidate their spatial-temporal patterns and key influencing factors and to reappraise the significance for regional carbon *** showed that,the fCO_(2) in rivers,lakes and reservoirs in China presented significant variability with large range of-379.3-4947.6,-160.1-785.0 and -74.0-1603.1 mg CO_(2)m-2h^(-1),*** median of the fCO_(2) in rivers was of 228.5 mg CO_(2)m^(-2)h^(-1),observably higher than these in lakes and reservoirs(26.0 and 28.3 mg CO_(2)m^(-2)h^(-1),respectively).The fCO_(2)in rivers and reservoirs exhibited similar decreasing trend from south to north as a result of universal climate restraint,the averaged fCO_(2) in Pearl River and Yangtze River basins showed much higher than that in Northeastern ***,the averaged fCO_(2) in the Mongolia-Xinjiang lake district and the Northeast lake district were higher than other lake districts,followed by the Qinghai-Xizang lake district,and the Eastern and Yungui lake district were generally low,contradicting the climatic *** water primary production enhancement resulting from human activities was the main driver of spatial variation in the fCO_(2) in ***,the fCO_(2) in rivers presented seasonal pattern with higher wet season than dry season,while opposite patterns were found in lakes and *** temperature,precipitation and water primary production were main ***,it showed pH was a key factor indicating the variability of the fCO_(2) levels either in rivers,lakes or in *** lakes,the fCO_(2) is closely linked to chlorophyll a(Chl-a)and dissolved oxygen(DO),whereas,the fCO_(2) in rivers is primarily associated with organic carbon(OC)and total nitrogen(TN),highlighting the dive

关键词： Rivers/lakes/reservoirs CO_(2) emission Spatio-temporal variability Influence factors Regional contribution

AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana

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Molecular Plant 2014年第1期7卷 206-217页

作者： Qing-Bo Yu Qian Ma Meng-Meng Kong Tuan-Tuan Zhao Xiao-Lei Zhang que zhou Chao Huang Kang Chong Zhong-Nan Yang Biology Department Life and Environmental College Shanghai Normal University Shanghai 200234 China National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Research Center for Molecular and Developmental Biology Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany the Chinese Academy of Sciences 100093 Beijing China

Plastid-encoded RNA polymerase （PEP） is closely associated with numerous factors to form PEP complex for plastid gene expression and chloroplast development. However, it is not clear how PEP complex are regulated in chloroplast. Here, one thioredoxin-like fold protein, Arabidopsis early chloroplast biogenesis 1 （AtECB1）, an allele of MRL7, was identified to regulate PEP function and chloroplast biogenesis. The knockout lines for AtECB1 displayed albino phenotype and impaired chloroplast development. The transcripts of PEP-dependent plastid genes were barely detected, suggesting that the PEP activity is almost lost in atecbl-1. Although AtECB1 was not identified in PEP complex, a yeast two-hybrid assay and pull-down experiments demonstrated that it can interact with Trx Z and FSD3, two intrinsic subunits of PEP complex, respectively. This indicates that AtECB1 may play a regulatory role for PEP-dependent plastid gene expression through these two subunits. AtECB1 contains a βαβαββα structure in the thioredoxin-like fold domain and lacks the typical C-X-X-C active site motif. Insulin assay demonstrated that AtECB1 harbors disulfide reductase activity in vitro using the purified recombinant AtECB1 protein. This showed that this thioredoxin-like fold protein, AtECB1 also has the thioredoxin activity. AtECB1 may play a role in thioredoxin signaling to regulate plastid gene expression and chloroplast development.

关键词： Arabidopsis Thioredoxin disulfide reductase activity chloroplast transcription chloroplast biogenesis.

Microstructure and thermal properties of dissimilar M300–CuCr1Zr alloys by multi-material laser-based powder bed fusion

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International Journal of Minerals,Metallurgy and Materials 2024年第1期31卷 118-128页

作者： Xiaoshuang Li Dmitry Sukhomlinov Zaiqing que Aerosint SA B-4040 HerstalBelgium Aalto University Department of Chemical and Metallurgical EngineeringEspoo 02150Finland VTT Technical Research Centre of Finland Espoo 02044Finland

Multi-material laser-based powder bed fusion (PBF-LB) allows manufacturing of parts with 3-dimensional gradient and additional functionality in a single step. This research focuses on the combination of thermally-conductive CuCr1Zr with hard M300 tool *** interface configurations of M300 on CuCr1Zr and CuCr1Zr on M300 were investigated. Ultra-fine grains form at the interface due to the low mutual solubility of Cu and steel. The material mixing zone size is dependent on the configurations and tunable in the range of0.1–0.3 mm by introducing a separate set of parameters for the interface layers. Microcracks and pores mainly occur in the transition *** of these defects, the thermal diffusivity of bimetallic parts with 50vol% of CuCr1Zr significantly increases by 70%–150%compared to pure M300. The thermal diffusivity of CuCr1Zr and the hardness of M300 steel can be enhanced simultaneously by applying the aging heat treatment.

关键词： multi-material additive manufacturing laser-based powder bed fusion thermal diffusivity dissimilar metals copper alloy

Torque effect on vibration behavior of high-speed train gearbox under internal and external excitations

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Railway Engineering Science 2024年第2期32卷 229-243页

作者： Yue zhou Xi Wang Hongbo que Rubing Guo Xinhai Lin Siqin Jin Chengpan Wu Yu Hou School of Mechanical Electronic and Control EngineeringBeijing Jiaotong UniversityBeijing 100044China CRRC Qishuyan Institute Co. Ltd.No.258Wuyi RoadQishuyanChangzhou 213011China Frontiers Science Center for Smart High-Speed Railway System Beijing Jiaotong UniversityBeijing 100044China

The high-speed train transmission system,experiencing both the internal excitation originating from gear meshing and the external excitation originating from the wheel-rail interaction,exhibits complex dynamic behavior in the actual service *** paper focuses on the gearbox in the high-speed train to carry out the bench test,in which various operat-ing conditions(torques and rotation speeds)were set up and the excitation condition covering both internal and external was *** responses on multiple positions of the gearbox were acquired in the test and the vibration behavior of the gearbox was ***,a stochastic excitation modal test was also carried out on the test bench under different torques,and the modal parameter of the gearbox was ***,the sweep frequency response of the gearbox under gear meshing excitation was analyzed through dynamic *** results showed that the torque has an attenuating effect on the amplitude of gear meshing frequency on the gearbox,and the effect of external excitation on the gearbox vibration cannot be ignored,especially under the rated operating *** was also found that the torque affects the modal param-eter of the gearbox *** torque has a great effect on both the gear meshing stiffness and the bearing stiffness in the transmission system,which is the inherent reason for the changed modal characteristics observed in the modal test and affects the vibration behavior of the gearbox consequently.

关键词： High-speed train Gearbox Bench test Vibration behavior Modal identification

Combustion mechanism of benzene in iron ore sintering process:experimental and simulation

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Journal of Iron and Steel Research International 2024年第1期31卷 195-203页

作者： Zhi-gang que Jin-ming Shi Xian-bin Ai Institute of Energy Research Jiangxi Academy of ScienceNanchang330096JiangxiChina Jiangxi Carbon Neutralization Research Center Nanchang330096JiangxiChina

Benzene is a typical component of volatile organic compounds(VOCs)in the iron ore sintering flue *** combustion behavior of benzene directly affects the emission of VOCs in iron ore sintering *** effects of temperature,benzene,and oxygen concentrations on the conversion ratio of benzene were investigated by experiments and numerical *** experiments were carried out in a tube reactor at temperatures of 773-1098 K,benzene concentrations of 0.01-0.03 vol.%,and oxygen concentrations of 10-21 vol.%.The numerical simulation was performed with the plug flow model in the CHEMKIN program based on a kinetic model that consists of 132 chemical species and 772 elementary step-like *** experimental results reveal that increasing the temperature and benzene concentration could signifi-cantly promote benzene *** is attributed to the increase in the reaction rates of all steps in the pathway for forming CO_(2)and H_(2)*** addition,due to the large equivalent ratio of oxygen to benzene,the conversion ratio of benzene remained constant at different oxygen *** simulation results were in good agreement with the experimental results and indicated that six elementary reactions dominated the formations of CO_(2)and H_(2)*** oxidations of C_(6)H_(5)O,CO,and C_(5)H_(4)O intermediates to CO_(2)were the limiting steps in the reaction pathways.

关键词： Benzene Volatile organic compound Iron ore sinter Combustion Simulation Temperature Oxygen

Fantastic voyage: The journey of NLRP3 inflammasome activation

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Genes & Diseases 2024年第2期11卷 819-829页

作者： Xiangyong que Sihao Zheng Qibin Song Huadong Pei Pingfeng Zhang Cancer Center Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Department of Oncology Georgetown Lombardi Comprehensive Cancer CenterGeorgetown University Medical CenterWashingtonDC 20057USA

NLRP3 inflammasome,an intracellular multiprotein complex,can be activated by a range of pathogenic microbes or endogenous hazardous *** activation results in the release of cytokines such as IL-1β and IL-18,as well as Gasdermin D which eventually causes *** activation of NLRP3 inflammasome is under strict control and regulation by numerous pathways and *** excessive activation can lead to a persistent inflammatory response,which is linked to the onset and progression of severe *** studies have revealed that the subcellular localization of NLRP3 changes significantly during the activation *** this review,we review the current understanding of the molecular mechanism of NLRP3 inflammasome activation,focusing on the subcellular localization of NLRP3 and the associated regulatory *** aim to provide a comprehensive understanding of the dynamic transportation,activation,and degradation processes of NLRP3.

关键词： Inflammasome activation Innate immunity NLRP3 NLRP3 inflammasome Subcellular localization