检索结果-南通市图书馆

Biophysical-driven piezoelectric and aligned nanofibrous scaffold promotes bone regeneration by re-establishing physiological electrical microenvironment

引用

Nano Research 2024年第8期17卷 7376-7393页

作者： Aoao Wang Xinbo Ma Yafeng Yang guoliang Shi Liwei Han Xiantong Hu Rui Shi Jun Yan quanyi guo Yantao Zhao Senior Department of Orthopedics the Fourth Medical Center of PLA General HospitalBeijing 100048China Department of Chemistry Capital Normal UniversityBeijing 100048China National Center for Orthopaedics Beijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalCapital Medical UniversityBeijing 100035China Xijing 986 Hospital Department The Fourth Military Medical UniversityXi'an 710032China Institute of Orthopedics Chinese PLA General HospitalBeijing Key Laboratory of Regenerative Medicine in OrthopedicsKey Laboratory of Musculoskeletal Trauma&War Injuries PLABeijing 100853China

The initial healing stages of bone fracture is a complex physiological process involving a series of spatially and temporally overlapping events,including pathogen clearance,immunological modulation,and *** this study,we have developed a piezoelectric and aligned nanofibrous scaffold composed of ZnO@PCL/PVDF with multiple antibacterial,immunomodulatory,and osteogenic effects using electrospinning *** scaffold’s piezoelectric signal output under ultrasound(US)control can be similar to the physiological electrical signals of healthy bone tissue,creating a truly biomimetic electrical microenvironment in the bone *** vitro studies have shown that ZnO@PCL/PVDF scaffold significantly enhances the proliferation,migration,and osteogenic differentiation of MC3T3-E1 cells under piezoelectric drive provided by ***,the scaffold exhibits inhibitory effects on the growth of *** and ***,as well as the ability to induce M2 macrophage polarization,indicating potent antibacterial and immunomodulatory *** vivo experiments demonstrated that the ZnO@PCL/PVDF scaffold can accelerate the repair of mandibular defects in rats,effectively inhibit bacterial colonization,and reduce inflammatory ***,this study confirms that the newly developed ZnO@PCL/PVDF scaffold effectively promotes bone repair by truly mimicking the endogenous electrical microenvironment and precisely regulating the temporospatial disorders of initial bone healing,thus providing a simple and effective solution for bone defects.

关键词： piezoelectricity bone defect bacterial infection ZnO nanoparticles immunomodulation osteogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

膝关节局灶性软骨损伤临床修复技术进展

引用

中国修复重建外科杂志 2024年第7期38卷 889-895页

作者：田广招李润萌杨永康宁超郭全义中国人民解放军总医院第一医学中心骨科研究所北京100853 南开大学医学院天津300071

目的总结临床实践中膝关节局灶性软骨损伤的经典和最新治疗技术,创建新的综合临床决策流程。方法广泛查阅国内外近年相关研究文献,总结膝关节局灶性软骨损伤各种治疗方法的优势和局限。结果目前临床治疗膝关节局灶性软骨损伤的手术方式... 详细信息

目的总结临床实践中膝关节局灶性软骨损伤的经典和最新治疗技术,创建新的综合临床决策流程。方法广泛查阅国内外近年相关研究文献,总结膝关节局灶性软骨损伤各种治疗方法的优势和局限。结果目前临床治疗膝关节局灶性软骨损伤的手术方式较多,各有利弊。对于4 cm^(2)伴有骨质丢失的软骨损伤患者首选OCA;而>4 cm^(2)不伴有骨质丢失的软骨损伤患者,手术选择包括ACI/基质材料复合ACI、OCA和PJAC。结论膝关节局灶性软骨损伤有很多治疗技术可供选择;治疗策略应基于病变大小、病变位置、软骨下骨累及程度以及文献中支持每种技术的证据水平进行选择。

关键词：关节软骨损伤软骨修复临床决策

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于低温沉积3D打印技术构建新型组织工程半月板支架的研究

引用

中国修复重建外科杂志 2024年第6期38卷 748-754页

作者：陈明学吴江殷瀚眭翔刘舒云郭全义首都医科大学附属北京积水潭医院矫形骨科北京100035 中国人民解放军总医院第一医学中心骨科研究所骨科再生医学北京市重点实验室全军骨科战创伤重点实验室北京100853

目的基于低温沉积3D打印技术构建新型组织工程半月板支架,评价该支架理化性质及生物相容性。方法取新鲜猪膝关节半月板,采用改良物理化学联合方法脱细胞处理,获得脱细胞半月板基质匀浆;经大体观察、HE及DAPI染色观察脱细胞效果,甲苯胺... 详细信息

目的基于低温沉积3D打印技术构建新型组织工程半月板支架,评价该支架理化性质及生物相容性。方法取新鲜猪膝关节半月板,采用改良物理化学联合方法脱细胞处理,获得脱细胞半月板基质匀浆;经大体观察、HE及DAPI染色观察脱细胞效果,甲苯胺蓝、番红O及天狼猩红染色评估黏多糖和胶原保留情况。然后制备脱细胞半月板基质生物墨水,通过低温沉积3D打印技术制备新型组织工程半月板支架。扫描电镜观察微观结构;与脂肪来源干细胞共培养后,采用细胞计数试剂盒8(cell counting kit 8,CCK-8)检测支架细胞相容性,死/活细胞染色和细胞骨架染色观察细胞活性和形态;植入大鼠皮下后组织学染色评估支架炎症细胞浸润与降解情况。结果脱细胞处理后半月板基质匀浆呈透明凝胶状,DAPI和组织学染色示免疫原性的核酸去除,同时黏多糖及胶原成分保留。采用低温沉积3D打印技术成功构建新型组织工程半月板支架,扫描电镜示支架呈分级大孔-微孔的微观结构;CCK-8检测示支架具有良好细胞相容性;死/活细胞染色示支架可有效维持细胞活性(>90%);细胞骨架染色示支架有利于细胞黏附和铺展;支架植入大鼠皮下1周后有轻度炎症反应,3周后未见明显炎症反应,并可见支架逐步降解。结论基于低温沉积3D打印技术构建的新型组织工程半月板支架具有分级大孔-微孔的微观结构和良好细胞相容性,有利于细胞黏附和生长,为下一步体内研究奠定基础。

关键词：组织工程支架低温沉积3D打印技术半月板脱细胞基质

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

甲基丙烯酰化透明质酸/脱细胞华通胶水凝胶支架的制备与表征

引用

中国组织工程研究 2024年第22期28卷 3517-3523页

作者：袁勋丁振罡付力伟吴江郑亚哲张智超田广招眭翔刘舒云郭全义贵州医科大学附属医院骨科贵州省贵阳市550004 解放军总医院第一医学中心骨科研究所骨科再生医学北京市重点实验室全军骨科战创伤重点实验室北京市100853

背景:随着组织工程为关节软骨损伤修复这一世界难题带来了新的希望,构建成分仿生的光固化3D打印水凝胶支架对软骨组织工程具有重要意义。目的:通过数字光处理3D打印技术构建成分仿生的甲基丙烯酰化透明质酸/脱细胞华通胶水凝胶支架,评... 详细信息

背景:随着组织工程为关节软骨损伤修复这一世界难题带来了新的希望,构建成分仿生的光固化3D打印水凝胶支架对软骨组织工程具有重要意义。目的:通过数字光处理3D打印技术构建成分仿生的甲基丙烯酰化透明质酸/脱细胞华通胶水凝胶支架,评价其生物相容性。方法:从人脐带中分离提取华通胶组织后进行脱细胞处理,冷冻干燥后磨成粉末,溶于PBS中制备50 g/L的脱细胞华通胶溶液。制备甲基丙烯酰化透明质酸,冻干后溶于PBS中制备50 g/L的甲基丙烯酰化透明质酸溶液。将脱细胞华通胶溶液与甲基丙烯酰化透明质酸溶液以体积比1∶1混合,加入光引发剂后作为生物墨水。通过数字光处理3D打印技术分别制备甲基丙烯酰化透明质酸水凝胶支架(记为HAMA水凝胶支架)与甲基丙烯酰化透明质酸/脱细胞华通胶水凝胶支架(记为HAMA/WJ水凝胶支架),表征支架的微观结构、溶胀性能、生物相容性与促软骨分化性能。结果与结论:①扫描电镜下见两组支架均呈三维立体的网状结构,其中HAMA/WJ水凝胶支架纤维连接更加均匀;两组支架均在10 h内达到溶胀平衡,HAMA/WJ水凝胶支架的平衡溶胀比低于HAMA水凝胶支架(P<0.05)。②CCK-8实验显示相较于HAMA水凝胶支架,HAMA/WJ水凝胶支架可促进骨髓间充质干细胞的增殖;死活染色显示骨髓间充质干细胞在两组支架上生长良好,并且HAMA/WJ水凝胶支架上的细胞立体分布均匀、细胞数量更多;鬼笔环肽染色显示相较于HAMA水凝胶支架,HAMA/WJ水凝胶支架上的骨髓间充质干细胞黏附与铺展更佳。③将骨髓间充质干细胞接种于两组支架后进行成软骨诱导培养,qRT-PCR检测结果显示,HAMA/WJ水凝胶支架组聚集蛋白聚糖、SOX9、Ⅱ型胶原mRNA表达量均高于HAMA水凝胶支架组(P<0.05,P<0.01)。④结果表明,数字光处理3D生物打印HAMA/WJ水凝胶支架可促进骨髓间充质干细胞的增殖、黏附及成软骨分化。

关键词：华通胶甲基丙烯酰化透明质酸水凝胶支架数字光处理3D打印软骨损伤骨髓间充质干细胞组织工程

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advancing drug delivery to articular cartilage:From single to multiple strategies

引用

药学学报:英文版 2023年第10期13卷 4127-4148页

作者： Tianyuan Zhao Xu Li Hao Li Haoyuan Deng Jianwei Li Zhen Yang Songlin He Shuangpeng Jiang Xiang Sui quanyi guo Shuyun Liu Institute of Orthopedics The First Medical CenterChinese PLA General HospitalBeijing Key Lab of Regenerative Medicine in OrthopedicsKey Laboratory of Musculoskeletal Trauma&War Injuries PLABeijing 100853China School of Medicine Nankai UniversityTianjin 300071China Musculoskeletal Research Laboratory Department of Orthopedics&TraumatologyThe Chinese University of Hong Kong999077Hong KongChina Arthritis Clinic&Research Center Peking University People’s HospitalPeking UniversityBeijing 100044China Department of Joint Surgery Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China

Articular cartilage(AC) injuries often lead to cartilage degeneration and may ultimately result in osteoarthritis(OA) due to the limited self-repair ability. To date, numerous intra-articular delivery systems carrying various therapeutic agents have been developed to improve therapeutic localization and retention, optimize controlled drug release profiles and target different pathological processes. Due to the complex and multifactorial characteristics of cartilage injury pathology and heterogeneity of the cartilage structure deposited within a dense matrix, delivery systems loaded with a single therapeutic agent are hindered from reaching multiple targets in a spatiotemporal matched manner and thus fail to mimic the natural processes of biosynthesis, compromising the goal of full cartilage regeneration. Emerging evidence highlights the importance of sequential delivery strategies targeting multiple pathological processes. In this review, we first summarize the current status and progress achieved in single-drug delivery strategies for the treatment of AC diseases. Subsequently, we focus mainly on advances in multiple drug delivery applications, including sequential release formulations targeting various pathological processes, synergistic targeting of the same pathological process, the spatial distribution in multiple tissues, and heterogeneous regeneration. We hope that this review will inspire the rational design of intraarticular drug delivery systems(DDSs) in the future.

关键词： Drug delivery systems Articular cartilage Cartilage injury Osteoarthritis Cartilage regeneration Multiple drug delivery strategy Biomaterials Therapeutic factors

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advances in regenerative medicine applications of tetrahedral framework nucleic acid-based nanomaterials: an expert consensus recommendation

引用

International Journal of Oral Science 2022年第4期14卷 439-460页

作者： Yunfeng Lin Qian Li Lihua Wang quanyi guo Shuyun Liu Shihui Zhu Yu Sun Yujiang Fan Yong Sun Haihang Li Xudong Tian Delun Luo Sirong Shi State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Oral and Maxillofacial Surgery West China Hospital of StomatologySichuan UniversityChengduChina School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative MoleculesInstitute of Translational MedicineShanghai Jiao Tong UniversityShanghaiChina The Interdisciplinary Research Center Shanghai Advanced Research InstituteChinese Academy of SciencesZhangjiang LaboratoryShanghaiChina Institute of Orthopedics Chinese PLA General HospitalBeijing Key Laboratory of Regenerative Medicine in OrthopedicsKey Laboratory of Musculoskeletal Trauma&War Injuries PLABejingChina Department of Burn Surgery The First Affliated Hospital of Naval Medical UniversityShanghaiChina National Engineering Research Center for Biomaterials Sichuan UniversityChengduChina College of Biomedical Engineering Sichuan UniversityChengduChina Jiangsu Trautec Medical Technology Company Limited ChangzhouChina Chengdu Jingrunze Gene Technology Company Limited ChengduChina

With the emergence of DNA nanotechnology in the 1980s, self-assembled DNA nanostructures have attracted considerable attention worldwide due to their inherent biocompatibility, unsurpassed programmability, and versatile functions. Especially promising nanostructures are tetrahedral framework nucleic acids(t FNAs), first proposed by Turberfield with the use of a one-step annealing approach. Benefiting from their various merits, such as simple synthesis, high reproducibility, structural stability, cellular internalization, tissue permeability, and editable functionality, t FNAs have been widely applied in the biomedical field as threedimensional DNA nanomaterials. Surprisingly, t FNAs exhibit positive effects on cellular biological behaviors and tissue regeneration,which may be used to treat inflammatory and degenerative diseases. According to their intended application and carrying capacity,t FNAs could carry functional nucleic acids or therapeutic molecules through extended sequences, sticky-end hybridization,intercalation, and encapsulation based on the Watson and Crick principle. Additionally, dynamic t FNAs also have potential applications in controlled and targeted therapies. This review summarized the latest progress in pure/modified/dynamic t FNAs and demonstrated their regenerative medicine applications. These applications include promoting the regeneration of the bone,cartilage, nerve, skin, vasculature, or muscle and treating diseases such as bone defects, neurological disorders, joint-related inflammatory diseases, periodontitis, and immune diseases.

关键词： tetrahedral summarized versatile

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nanoparticle–Cartilage Interaction: Pathology-Based Intra-articular Drug Delivery for Osteoarthritis Therapy

引用

Nano-Micro Letters 2021年第10期13卷 29-76页

作者： Xu Li Bingyang Dai Jiaxin guo Lizhen Zheng quanyi guo Jiang Peng Jiankun Xu Ling Qin Musculoskeletal Research Laboratory Department of Orthopedics and TraumatologyThe Chinese University of Hong KongSha TinHong KongSARPeople’s Republic of China Joint Laboratory of Chinese Academic of Science and Hong Kong for Biomaterials The Chinese University of Hong KongSha TinHong KongSARPeople’s Republic of China Beijing Key Lab of Regenerative Medicine in Orthopedics Key Laboratory of Musculoskeletal Trauma and War Injuries PLAInstitute of OrthopedicsChinese PLA General HospitalBeijingPeople’s Republic of China Innovative Orthopedic Biomaterial and Drug Translational Research Laboratory Li Ka Shing Institute of Health SciencesThe Chinese University of Hong KongSha TinHong KongSARPeople’s Republic of China

Osteoarthritis is the most prevalent chronic and debilitating joint disease,resulting in huge medical and socioeconomic ***-articular administration of agents is clinically used for pain ***,the effectiveness is inapparent caused by the rapid clearance of *** overcome this issue,nanoparticles as delivery systems hold considerable promise for local control of the pharmacokinetics of therapeutic *** the therapeutic programs are inseparable from pathological progress of osteoarthritis,an ideal delivery system should allow the release of therapeutic agents upon specific features of *** this review,we firstly introduce the pathological features of osteoarthritis and the design concept for accurate localization within cartilage for sustained drug ***,we review the interactions of nanoparticles with cartilage microenvironment and the rational ***,we highlight advances in the therapeutic schemes according to the pathology ***,armed with an updated understanding of the pathological mechanisms,we place an emphasis on the development of“smart”bioresponsive and multiple modality nanoparticles on the near horizon to interact with the pathological *** anticipate that the exploration of nanoparticles by balancing the efficacy,safety,and complexity will lay down a solid foundation tangible for clinical translation.

关键词： Nanoparticle Drug delivery Osteoarthritis Articular cartilage Nanomedicine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Characterization of decellularized scaffold derived from porcine meniscus for tissue engineering applications

引用

Frontiers of Materials Science 2016年第2期10卷 101-112页

作者： Shuang GAO Zhiguo YUAN Tingfei XI Xiaojuan WEI quanyi guo Center for Biomaterial and Tissue Engineering Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China Key Laboratory of PLA Institute of Orthopaedics Chinese PLA General Hospital Beijing 100853 China Department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China

Menisci are fundamental fibrocartilaginous organs in knee joints. The injury in meniscus can impair normal knee function and predisposes patients to osteoarthritis. This study prepared decellularized meniscus scaffolds using a 1% （w/w） sodium dodecyl sulfate solution and sufficient rinsing steps. Complete cell removal was verified by hematoxylin and eosin staining and DNA content assay. Decellularized menisci had accordant tension properties to intact ones, but with declined compression properties. This occurred because the collagen fiber was not damaged but glycosami- noglycans was significantly lost during the decellularization process, which was confirmed by biochemical assay and histology staining. In vitro cytotoxicity assay demonstrated that decellularized meniscus scaffolds have no toxicity on L929 murine fibroblasts and porcine chondrocytes. Further experiment showed that porcine chondrocytes could adhere and proliferate on the scaffold surface, and some cells even could infiltrate into the scaffold. All results showed the potential of this decellularized meniscus to be the scaffolds in tissue engineering.

关键词： meniscus scaffold decellularization biomechanical cytotoxicity tissueengineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Key considerations on the development of biodegradable biomaterials for clinical translation of medical devices:With cartilage repair products as an example

引用

Bioactive Materials 2022年第3期7卷 332-342页

作者： Li Wang Xiaolei guo Jiaqing Chen Zhen Zhen Bin Cao Wenqian Wan Yuandong Dou Haobo Pan Feng Xu Zepu Zhang Jianmei Wang Daisong Li quanyi guo Qing Jiang Yanan Du Jiakuo Yu Boon Chin Heng Qianqian Han Zigang Ge Department of Biomedical Engineering College of EngineeringPeking UniversityBeijingPR China Center for Medical Device Evaluation National Medical Products AdministrationBeijingPR China Jiangsu DissueTech Medical Technology Co.Ltd|DeJian Group SuzhouPR China State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghaiPR China Research Center for Human Tissue and Organs Degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of ScienceShenzhenPR China Bioinspired Engineering and Biomechanics Center(BEBC) MOE Key Laboratory of Biomedical Information EngineeringSchool of Life Science and TechnologyXi’an Jiaotong UniversityXi’anPR China Beijing Institute of Science and Technology Evaluation BeijingPR China Institute of Orthopedics Chinese PLA General HospitalBeijingPR China Department of Sports Medicine and Adult Reconstructive Surgery State Key Laboratory of Pharmaceutical BiotechnologyThe Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical SchoolNanjingPR China Department of Biomedical Engineering School of MedicineTsinghua UniversityBeijingPR China Knee Surgery Department of the Institute of Sports Medicine Beijing Key Laboratory of Sports InjuriesPeking University Third HospitalBeijingPR China School of Stomatology Peking UniversityBeijingPR China National Institutes for Food and Drug Control BeijingPR China Department of Biomedical Engineering Institute of Future TechnologyPeking UniversityBeijingPR China

With the interdisciplinary convergence of biology,medicine and materials science,both research and clinical translation of biomaterials are progressing at a rapid ***,there is still a huge gap between applied basic research on biomaterials and their translational products-medical devices,where two significantly different perspectives and mindsets often work independently and non-synergistically,which in turn significantly increases financial costs and research *** this gap is well-known and often criticized in the biopharmaceutical industry,it is gradually *** this article,we critically examine the developmental pipeline of biodegradable biomaterials and biomaterial-based medical device *** based on clinical needs,market analysis,and relevant regulations,some ideas are proposed to integrate the two different mindsets to guide applied basic research and translation of biomaterial-based products,from the material and technical *** repair substitutes are discussed here as an ***,this will lay a strong foundation for biomaterial research and clinical translation,while reducing the amount of extra research effort and funding required due to the dissonance between innovative basic research and commercialization pipeline.

关键词： Biomaterials Polymer Translation Medical devices Regulation Clinical needs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acellular nerve allograft promotes selective regeneration

引用

Neural Regeneration Research 2011年第4期6卷 257-261页

作者： Haili Xin Guanjun Wang Xinrong He Jiang Peng quanyi guo Wenjing Xu Bureau of Drugs Department of Surgery Chinese PLA General Hospital Beijing 100853 China Department of Spine Surgery General Hospital of Chinese Armed Police Forces Beijing 100039 China Institute of Orthopedics General Hospital of Chinese PLA Beijing 100853 China

Acellular nerve ailograft preserves the basilar membrane tube and extracellular matrix, which promotes selective regeneration of neural defects via bridging. In the present study, a Sprague Dawley rat sciatic nerve was utilized to prepare acellular nerve allografts through the use of the chemical extraction method. Subsequently, the allograft was transplanted into a 10-mm sciatic nerve defect in Wistar rats, while autologous nerve grafts from Wistar rats served as controls. Compared with autologous nerve grafts, the acellular nerve allografts induced a greater number of degenerated nerve fibers from sural nerves, as well as a reduced misconnect rate in motor fibers, fewer acetylcholine esterase-positive sural nerves, and a greater number of carbonic anhydrase-positive sensory nerve fibers. Results demonstrated that the acellular nerve allograft exhibited significant neural selective regeneration in the process of bridging nerve defects.

关键词： acellular nerve allograft selective regeneration peripheral nerve tissue engineering neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：