检索结果-南通市图书馆

Asian Journal of Andrology 2018年第6期20卷 608-614页

作者： qing-qing Yin Lin-Hui xu Mi Zhang Chen xu Institute of Life Sciences Chongqing Medical UniversityChongqing 400016China.

The autonomic nervous system contributes to prostate cancer proliferation and metastasis. However, the exact molecular mechanism remains unclear. In this study, muscarinic acetylcholine receptor M1 （CHRM1） expression was measured via immunohistochemical analysis in human prostate cancer tissue array slides. PC-3, LNCaP, and A549 cells were treated with pirenzepine or carbachol, and the cell migration and invasion abilities were evaluated. Western blotting and quantitative real-time PCR were performed to measure GLI family zinc finger 1 （GLI1）, patched 1 （PTCH1）, and sonic hedgehog （SHH） expression levels. High expression of CHRM1 was found in early-stage human prostate cancer tissues. In addition, the selective CHRM1 antagonist pirenzepine inhibited PC-3, LNCaP, and A549 cell migration and invasion, but the agonist carbachol promoted the migration and invasion of these three cell lines. Muscarinic signaling can be relayed by hedgehog signaling. These data show that CHRM1 is involved in the regulation of prostate cancer migration and invasion through the hed^eho~ si^nalin~ ~athwav.

关键词： hedgehog signaling muscarinic acetylcholine receptor M1 (CHRM1) metastasis prostate cancer

Tuning the Stability of the Polyplex Nanovesicles of Oligonucleotides via a Zinc(Ⅱ)-Coordinative Strategy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2022年第9期40卷 1034-1042页

作者： quan Zhou Xiang Li Jia-Jia Xiang You-qing Shen Zhejiang Key Laboratory of Smart Biomaterials and Center for Bionanoengineering College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027China Department of Cell Biology School of Basic Medical SciencesZhejiang UniversityHangzhou 310058China The Forth Affiliated Hospital Zhejiang University School of MedicineYiwu 322000China Hangzhou Global Scientific and Technological Innovation Center Hangzhou 311215China

Oligonucleotide therapeutics have great potential to target the currently undruggable genes and to generate entirely new therapeutic paradigms in multiple types of disease,thus having attracted much attention in recent ***,their applications are greatly hindered by a lack of safe and efficient oligonucleotide-delivery *** nanovesicles formed from oligonucleotides and the cationic block have shown exceptional features for the delivery of therapeutic oligonucleotides and other ***,these polyplex nanovesicles are deeply fraught with difficulty in tolerating physiological ionic *** by the high binding ability between the dipicolylamine(DPA)/zinc(Ⅱ)complex and the phosphodiester moieties of oligonucleotides,herein,we designed a coordinative cationic block to solve the intrinsic stability ***,we found the stability of the resulted polyplex nanovesicles could be easily tuned by the content of coordinated zinc *** vitro cellular studies implied that the prepared zinc(Ⅱ)-coordinative polyplex nanovesicles preferred to retain in the lysosomes upon internalization,making them ideal delivery candidates for the lysosome-targeting oligonucleotide therapeutics.

关键词： Oligonucleotide therapeutics Polyplex nanovesicles Coordinative cationic block Lysosome-targeting

CHARACTERISTICS OF CORRELATION AND SPECTRUM BETWEEN WALL FLUCTUATING PRESSURE AND FLUCTUATING VELOCITY OF IMPINGING JET

在线全文

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 2002年第3期14卷 21-28页

作者： Dai, Guang-qing Yang, Yong-quan Yang, qing Wang, Hai-Yun Hydraulics Lab. of High Speed Flows Sichuan Univ. Chengdu 610065 China

The correlation, between wall fluctuating pressure and fluctuating velocity, on the bed of plunging pool under the action of single and twin impinging jet, and their spectrum and turbulent scale are investigated in the paper by using 2-D LDA and the dynamic pressure scanning system of multiple points. It is found that the cross correlation between the horizontal fluctuating velocity and the fluctuating pressure of twin jet is bigger than that of single jet, and the spectrums of horizontal fluctuating velocity and fluctuating pressure obviously have approximately same dominant frequency. The relationship between the fluctuation pressure coefficient on wall and the turbulent intensity near the wall can be described with logarithmic curve.

关键词： Hydraulic structures Pressure Pressure measurement Turbulence Velocity

A highly sensitive dopamine sensor based on a polyaniline/reduced graphene oxide/Nafion nanocomposite

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2017年第1期28卷 41-48页

作者： Li-qing Xie Yan-Hui Zhang Fei Gao quan-An Wu Piao-Yang xu Shan-Shan Wang Ning-Ning Gao qing-Xiang Wang College of Chemistry and Environment Minnan Normal University Zhangzhou 363000 China Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology Minnan Normal University Zhangzhou 363000 China

A nanocomposite of polyaniline/reduced graphene oxide（PANI-r GO） was synthesized using a hydrothermal method. The product was characterized by FT-IR, Raman spectra, XRD, SEM and *** the hybrid material of PANI-r GO and Nafion（PANI-rGO-NF） was prepared and used to modify glassy carbon electrode for the trace determination of dopamine（DA） employing differential pulse voltammetry（DPV）. It was found that the hybrid material showed good catalytic activity toward the oxidation of DA, and no response to ascorbic acid（AA） and uric acid（UA） was observed, suggesting a high selectivity of the sensor toward DA. The peak currents were linearly correlated with the concentration of DA in the range from 0.05 mmol/L to 60.0 mmol/L（R = 0.996） and 60.0 mmol/L to 180.0 mmol/L（R = 0.996） with a detection limit of 0.024 mmol/L（S/N = 3）. The modified electrode also exhibited excellent repeatability and stability.

关键词： Dopamine Graphene oxide Electrochemical Nation Polyaniline

Effect of HSPB9 on Apoptosis of DF-1 Cells

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2019年第2期32卷 107-120页

作者： xu Yong Jie HU Mei Ling ZHOU Liang Hui WANG Qi ZHANG Xi quan LUO qing Bin Department of Animal Genetics Breeding and Reproduction College of Animal Science/Key Laboratory of Chicken Genetics Breeding and Reproduction Ministry of Agriculture South China Agricultural University

Objective Our aim was to explore whether heat stress protein(HSP) 9 preferentially expresses under heat stress and affects the expression of other heat stress proteins as well as to explore the effect of HSPB9 overexpression and knockdown on apoptosis in DF-1. Methods We used gene cloning to construct an overexpression vector of the target gene, and synthesized the target gene interference fragment to transfect the chicken fibroblast cell line. Gene and protein expression, as well as apoptosis, were detected by RT-qPCR, Western blot, and flow cytometry. Results Chicken DF-1 cells showed an early state of apoptosis in the early stages of HSPB9 overexpression. In the later stages, as HSPB9 expression increased, the cells showed inhibition of apoptosis. When the cells were under heat stress, HSPB9 expression was much higher and earlier than the expression of HSPB1 and HSPA2. In addition, high expression of HSPB9 had a negative effect on HSPB1 and HSPA2 expression. This negative feedback decreased the percentage of early stages of apoptotic cells and promoted cell survival. Conclusion HSPB9 expression, although rapid, is detrimental to cell survival early during its overexpression. In heat stress, HSPB9 overexpression, while inhibiting the expression of HSPA2 and HSPB1, is beneficial to cell survival.

关键词： Heat stress HSPB9 HSPB1 HSPA2 Apoptosis

On GF(p)-linear complexities of binary sequences

在线全文

学校读者我要写书评

暂无评论

The Journal of China Universities of Posts and Telecommunications 2009年第4期16卷 112-115,124页

作者： xu Li-qing Software Engineering Institute East China Normal University Shanghai 200062 China

Several geometric sequences have very low linear complexities when considered as sequences over GF（p）, such as the binary sequences of period q^n - 1 constructed by Chan and Games [1-2] （q is a prime power p^m, p is an odd prime） with the maximal possible linear complexity q^n-1 when considered as sequences over GF（2）. This indicates that binary sequences with high GF（2） linear complexities LC2 and low GF（p）-linear complexities LCp are not secure for use in stream ciphers. In this article, several lower bounds on the GF（p）-linear complexities of binary sequences is proved and the results are applied to the GF（p）-linear complexities of Blum-Blum-Shub, self-shrinking, and de Bruijn sequences. A lower bound on the number of the binary sequences with LC2 〉 LCD is also presented.

关键词： cryptography stream cipher binary sequences GF(2) linear complexity GF(p)-linear complexity

Penile augmentation with injectable hyaluronic acid gel:an alternative choice for small penis syndrome

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2022年第6期24卷 601-606页

作者： Chun-Long Zhang Yuan quan He Li qing Li Wen-Jun Bai Tao xu Xiao-Wei Zhang Department of Urology Peking University People's HospitalBeijing 100044China

There is no well-established procedure for the management of small penis syndrome(SPS),especially when psychological interventions *** study aimed at systematically evaluating the physical and psychological benefits of penile augmentation(PA)using injectable hyaluronic acid(HA)***-eight patients receiving PA with HA gel from January 2017 to March 2020 were included and followed up for 1 *** size,erectile function,and psychological burden measured by the Index of Male Genital Image(IMGI),Index of International Erectile Function(lIEF),and Beliefs about Penis Size(BPAS),respectively,were assessed at the beginning and at 1,3,6,and 12 months *** volume of HA gel injected was 21.5±3.7 *** to baseline data,flaccid penile girth and length significantly increased by 3.41±0.95 cm(P<0.01)and 2.55±0.55 cm(P<0.01)at the 1^(st)month postinjection,*** the endpoint,despite attenuations,statistically significant improvements in flaccid penis size were still obtained,namely 2.44±1.14 cm in girth(P<0.01)and 1.65±0.59 cm in length(P<0.01).Similarly,erectile penile girth statistically increased by 1.32±1.02 cm(P<0.01)at the 1^(st)month but were only 0.80±0.54 cm bigger than baseline(P

关键词： hyaluronic acid penile augmentation small penis syndrome

Color routing at the nanoscale

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Light(Science & Applications) 2019年第1期8卷 663-664页

作者： qing-Hua xu Department of Chemistry National University of Singapore3 Science Drive 3Singapore 117543Singapore

Two types of color routing effects were observed in silver nanorods on the single particle level as a result of distinct far-field behaviors of different multipolar plasmon modes,which enables tunable color sorting wi... 详细信息

关键词： routing nanorods silver

Evaluation of terrestrial radiation in the Chengdu Plain using 1/250,000-scale geochemical prospecting data

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nuclear Science and Techniques 2017年第10期28卷 31-36页

作者： qing-Xian Zhang Jian-Kun Zhao Yi Gu Liang-quan Ge Yuan-qing Huang Sichuan-Provincial Key Laboratory of Nuclear Technologyin Geosciences Chengdu University of TechnologyChengdu 610059China Sichuan Nuclear Geology Institute Chengdu 610061China

Based on the geochemical prospecting data, we have calculated the gamma absorbed dose rates in air 1 m above the ground surface in the Chengdu Plain, analyzed the relationship between the geological conditions and the distribution of the natural radionuclides, and preliminarily studied the influence of the geological conditions on the terrestrial radiation level in the Chengdu Plain. The result shows that the terrestrial radiation level in the Chengdu Plain is slightly lower than the average values of China and varies greatly according to the complex geological conditions.

关键词：成都平原地面辐射地球化学找矿评价天然放射性核素地质条件辐射水平普查数据

An end-to-side suspender pancreaticojejunostomy: A new invagination pancreaticojejunostomy

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2018年第2期17卷 163-168页

作者： Bin Li Chang xu Zhi-quan Qiu Chen Liu Bin Yi Xiang-Ji Luo Xiao-qing Jiang Department of Biliary Tract Surgery Eastern Hepatobiliary Surgery Hospital Secondary Military Medical University

Background: Postoperative pancreatic fistula(POPF) is a severe complication of the pancreaticoduodenectomy(PD). Recently, we introduced a method of suspender pancreaticojejunostomy(PJ) to the PD. In this study, we retrospectively analyzed various risk factors for complications after PD. We also introduced and assessed the suspender PJ to demonstrate its ***: Data from 335 patients with various periampullary lesions, who underwent the Whipple procedure(classic Whipple procedure or pylorus-preserving) PD by either traditional end-to-side invagination PJ or suspender PJ, were analyzed. The correlation between either perioperative or postoperative complications and corresponding PD approaches was evaluated by univariate ***: A total of 147 patients received the traditional end-to-side invagination PJ, and 188 patients were given the suspender PJ. Overall, 51.9% patients had various complications after PD. The mortality rate was 2.4%. The POPF incidence in patients who received the suspender PJ was 5.3%, which was significantly lower than those who received the traditional end-to-side invagination PJ(18.4%)(P < 0.001).Univariate analysis showed that PJ approach and the pancreas texture were significantly associated with the POPF incidence rate(P < 0.01). POPF was a risk factor for both postoperative abdominal cavity infection(OR = 8.34, 95% CI: 3.99–17.42, P < 0.001) and abdominal cavity hemorrhage(OR = 4.86, 95% CI:1.92–12.33, P = 0.001).Conclusions: Our study showed that the impact of the pancreas texture was a major risk factor for pancreatic leakage after a PD. The suspender PJ can be easily accomplished and widely applied and can effectively decrease the impact of the pancreas texture on pancreatic fistula after a PD and leads to a lower POPF incidence rate.

关键词： Pancreaticoduodenectomy Pancreaticojejunostomy Postoperative pancreatic fistula