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Plant and Yeast NHX Antiporters: Roles in Membrane Trafficking

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Journal of Integrative Plant Biology 2012年第2期54卷 66-72页

作者： quan-Sheng qiu School of Life Sciences Lanzhou University222 South Tianshui Rd.Lanzhou 730000China

The plant NHX gene family encodes Na＋/H＋ antiporters which are crucial for salt tolerance, potassium homeostasis and cellular pH regulation. Understanding the role of NHX antiporters in membrane trafficking is becoming an increasingly interesting subject of study. Membrane trafficking is a central cellular process during which proteins, lipids and polysaccharides are continuously exchanged among membrane compartments. Yeast ScNhxlp, a prevacuole/ vacuolar Na＋/H＋ antiporter, plays an important role in regulating pH to control trafficking out of the endosome. Evidence begins to accumulate that plant NHX antiporters might function in regulating membrane trafficking in plants.

关键词： Na+(K+)/H+ antiporter membrane trafficking yeast Arabidopsis.

A new real-time algorithm for off-road terrain estimation using laser data

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Science in China(Series F) 2009年第9期52卷 1658-1667页

作者： qiu quan YANG TangWen HAN JianDa State Key Laboratory of Robotics Shenyang Institute of Automation Chinese Academy of Sciences Shenyang 110016 China Graduate University of Chinese Academy of Sciences Beijing 100049 China School of Computer and Information Technology Beijing Jiao Tong University Beijing 100044 China

Gaussian mixture algorithm （GMA） is an effective approach for off-road terrain estimation, but still suffers from some difficulties in practical applications, such as complex calculation and object abstraction. In this paper, GMA is modified to improve its real-time performance and to provide it with a potential ability of obstacle detection. First, a selection window is designed based on the dominant-ellipse-principle to limit the probability distribution area of each measurement point, therefore avoiding the calculation on the cells outside the dominant ellipse. Second, a clustering approach is proposed in order to distinguish objects efficiently and decrease the operation area of one laser scan. Third, a virtual point vector is introduced to further reduce the computational load of the mean square error matrix. The modified GMA is experimented on a tracked mobile robot, and its improved performance is shown in comparison to the original GMA.

关键词： real-time off-road terrain estimation laser data

一株小菇属天麻萌发菌原生质体的制备、再生和应用

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陕西科技大学学报 2025年第1期43卷 58-66页

作者：李宏敬李锡琴粟忠祥仇全雷雷云霆西南林业大学林学院云南昆明650224 中国科学院昆明植物研究所云南昆明650201 昆明市林草局西山林场云南昆明650199 西藏波密高原藏天麻产业开发有限公司西藏林芝860318

小菇属真菌(Mycena spp.)遗传转化体系是研究其促进天麻种子萌发机制的重要基础,而其原生质体的制备和再生是该体系构建的必要条件.以小菇菌株MY004为实验材料,通过单因素分析和正交试验策略,探究其原生质体制备和再生的影响因素,构建... 详细信息

小菇属真菌(Mycena spp.)遗传转化体系是研究其促进天麻种子萌发机制的重要基础,而其原生质体的制备和再生是该体系构建的必要条件.以小菇菌株MY004为实验材料,通过单因素分析和正交试验策略,探究其原生质体制备和再生的影响因素,构建小菇原生质体制备和再生体系.结果表明,在含10 mM MES和0.7 M甘露醇的酶解缓冲液(pH=6)中,小菇MY004菌丝被10 mg/mL真菌溶壁酶在24℃条件下酶解3 h,其原生质体的释放率较高;经液体再生培养基(pH=5)培养3天,其原生质体再生效率可达2%.利用该体系,高效地完成了含报告基因eGFP转基因小菇的菌株纯化.小菇原生质体制备和再生体系可用于小菇遗传转化体系的快速构建,也将为小菇与天麻互作分子机制的研究提供基础平台.

关键词：小菇原生质体制备原生质体再生转基因菌株纯化

Implementation of Common Platform for Two-Person Game

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Implementation of Common Platform for Two-Person Game

Research on Techniques of Computer Game

第25届中国控制与决策会议

作者： Hongkun qiu Fuxiang Gao Yajie Wang Xuxu Song quan qiu Yuxia Sun Engineering Training Center Shenyang Aerospace University Institute of Information Science and Engineering Northeastern University School of Information Science and Engineering Shenyang ligong University

ISBN: (纸本)9781467355339

Computer Games（CG） is considered to be one of the most challenging research directions in the field of artificial intelligence. Though it is one of the most active research areas in recent years, there are less researches about the platform for CG. So some technologies about common platform for two-person game are researched. According to the characteristics of computer game, such as Dots and Boxes, Surakarta, Connect-six and Amazons, an application example was designed by using C/C++ language. The main process were listed as follow: Firstly of all, specified concepts and routines for interface, then defined some important data, included data structures, object classes, variables and functions Secondly introduced the expression of chess board and pieces in the computer. And then created input and output channel for searching engine. So a common platform of two-person player game was implemented. Finally, a series of experimentation were done. And software testing are added in the software development process. The experimentation results proved that this platform is effective for computer game and contributed to reduce subsequent development cost.

关键词： Computer Game Common Platform Search Engine User Interface Input and Output Channel

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Research on Techniques of Computer Game

V-ATPase,ScNhxlp and Yeast Vacuole Fusion

第25届中国控制与决策会议

作者： Hongkun qiu Fuxiang Gao Yajie Wang Yan Ge quan qiu Huimin Sun Engineering Training Center Shenyang Aerospace University Institute of Information Science and Engineering Northeastern University School of Information Science and Engineering Shenyang ligong University

ISBN: (纸本)9781467355339

Computer game is regarded as a new research field of Artificial Intelligence. It’s very difficult to design and implement a high-level computer game system. Some techniques of computer game were presented with an example from a chess game-Connect-six. Firstly, introduced the game tree and searching techniques. Secondly, the key technologies for computer games were discussed, including establishment and application of Hash Table, improvement the efficiency of pruning. Finally, some contrasting experiments were performed to test the performance of the program which used the previously mentioned techniques. Z he experiments’ results indicated that these techniques are very useful for computer game. And they have been confirmed by a series of competitions and tests. By these researches, a high-level game system can be produced and the results and conclusions of this paper also have some reference values to other computer game procedures.

关键词： Computer Game Minimax Algorithm Alpha-Beta Pruning Hash Table Evaluation

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Journal of Genetics and Genomics 2012年第4期39卷 167-171页

作者： quan-Sheng qiu School of Life Sciences Lanzhou UniversityLanzhouGansu 730000China

Membrane fusion is the last step in trafficking pathways during which membrane vesicles fuse with target organelles to deliver cargos. It is a central cellular reaction that plays important roles in signal transduction, protein sorting and subcellular compartmentation. Recent progress in understanding the roles of ion transporters in vacuole fusion in yeast is summarized in this article. It is becoming increasingly evident that the vacuolar proton pump V-ATPase and vacuolar Na＋/H＋ antiporter ScNhxlp are key components of the vacuole fusion machinery in yeast. Yeast ScNhxlp regulates vacuole fusion by controlling the luminal pH. V-ATPases serve a dual role in vacuolar integrity in which they regulate both vacuole fusion and fission reactions in yeast. Fission defects are epistatic to fusion defects. Vacuole fission depends on the proton translocation activity of the V-ATPase; by contrast, the fusion reaction does not need the transport activity but requires the physical presence of the proton pump. V0, the membrane-integral sector of the V-ATPase, forms trans-complexes between the opposing vacuoles in the terminal phase of vacuole fusion where the V0 trans-complexes build a continuous proteolipid channel at the fusion site to mediate the bilayer fusion.

关键词： ScNhxlp V-ATPase Vacuole fusion Fission Yeast

Mn-mediated reductive C(sp3)–Si coupling of activated secondary alkyl bromides with chlorosilanes

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Chinese Chemical Letters 2022年第12期33卷 5061-5064页

作者： Liangliang Qi Xiaobo Pang Kai Yin qiu-quan Pan Xiao-Xue Wei Xing-Zhong Shu State Key Laboratory of Applied Organic Chemistry(SKLAOC) College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China School of Chemistry and Chemical Engineering Southeast UniversityJiangsu Optoelectronic Functional Materials and Engineering LaboratoryNanjing 211189China Zhejiang Nanjiao Chemistry Co. Ltd.Shangyu Economic and Technological Development ZoneShangyu 312369China

The construction of secondary alkylsilanes is a challenging subject in the synthetic *** cross-coupling provides a practical solution to address this problem,but it typically relies on organometallic ***,we report an Mn-mediated reductive C(sp^(3))-Si coupling to synthesize these compounds from alkyl and silyl *** approach avoids the requirement for activation of Si-Cl by transition metals and thus allows for the coupling of various common *** reaction proceeds under mild conditions and shows good functional group *** method offers access toα-silylated organophosphorus and sulfones with a scope that is complementary to those obtained from the established methods.

关键词： Organosilanes Alkylsilanes Reductive coupling Chlorosilanes Organophosphorus Sulfones

Screening of Ginkgo Individuals with Superior Growth Structural Characteristics in Different Genetic Groups Using Terrestrial Laser Scanning(TLS)Data

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Plant Phenomics 2023年第4期5卷 714-727页

作者： Wen Gao Xiaoming Yang Lin Cao Fuliang Cao Hao Liu quan qiu Meng Shen Pengfei Yu Yuhua Liu Xin Shen Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjingJiangsu 210037PR China College of Forestry and Landscape Architecture South China Agricultural UniversityGuangzhouGuangdong 510642PR China Suining County Runqi Investment Co.Ltd. XuzhouJiangsu 221200PR China Jiangsu Vocational College of Agriculture and Forestry ZhenjiangJiangsu 212400PR China

With the concept of sustainable management of plantations,individual trees with excellent characteristics in plantations have received attention from *** improve and maintain long-term productivity,accurate and high-throughput access to phenotypic characteristics is essential when establishing breeding ***,genetic diversity is also an important issue that must be considered,especially for plantations without seed source *** study was carried out in a ginkgo timber *** used simple sequence repeat(SSR)markers for genetic background analysis and high-density terrestrial laser scanning for growth structural characteristic extraction,aiming to provide a possibility of applying remote sensing approaches for forest ***,we analyzed the genetic diversity and population structure,and grouped individual trees according to the genetic ***,the growth structural characteristics(height,diameter at breast height,crown width,crown area,crown volume,height to living crown,trunk volume,biomass of all components)were ***,individual trees in each group were comprehensively evaluated and the best-performing ones were *** illustrate that terrestrial laser scanning(TLS)point cloud data can provide nondestructive estimates of the growth structural characteristics at fine *** the ginkgo plantation containing high genetic diversity(average polymorphism information content index was 0.719)and high variation in growth structural characteristics(coefficient of variation ranged from 21.822%to 85.477%),11 excellent individual trees with superior growth were *** study guides the scientific management of plantations and also provides a potential for applying remote sensing technologies to accelerate forest breeding.

关键词： forest breeding crown

An end-to-side suspender pancreaticojejunostomy: A new invagination pancreaticojejunostomy

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Hepatobiliary & Pancreatic Diseases International 2018年第2期17卷 163-168页

作者： Bin Li Chang Xu Zhi-quan qiu Chen Liu Bin Yi Xiang-Ji Luo Xiao-Qing Jiang Department of Biliary Tract Surgery Eastern Hepatobiliary Surgery Hospital Secondary Military Medical University

Background: Postoperative pancreatic fistula(POPF) is a severe complication of the pancreaticoduodenectomy(PD). Recently, we introduced a method of suspender pancreaticojejunostomy(PJ) to the PD. In this study, we retrospectively analyzed various risk factors for complications after PD. We also introduced and assessed the suspender PJ to demonstrate its ***: Data from 335 patients with various periampullary lesions, who underwent the Whipple procedure(classic Whipple procedure or pylorus-preserving) PD by either traditional end-to-side invagination PJ or suspender PJ, were analyzed. The correlation between either perioperative or postoperative complications and corresponding PD approaches was evaluated by univariate ***: A total of 147 patients received the traditional end-to-side invagination PJ, and 188 patients were given the suspender PJ. Overall, 51.9% patients had various complications after PD. The mortality rate was 2.4%. The POPF incidence in patients who received the suspender PJ was 5.3%, which was significantly lower than those who received the traditional end-to-side invagination PJ(18.4%)(P < 0.001).Univariate analysis showed that PJ approach and the pancreas texture were significantly associated with the POPF incidence rate(P < 0.01). POPF was a risk factor for both postoperative abdominal cavity infection(OR = 8.34, 95% CI: 3.99–17.42, P < 0.001) and abdominal cavity hemorrhage(OR = 4.86, 95% CI:1.92–12.33, P = 0.001).Conclusions: Our study showed that the impact of the pancreas texture was a major risk factor for pancreatic leakage after a PD. The suspender PJ can be easily accomplished and widely applied and can effectively decrease the impact of the pancreas texture on pancreatic fistula after a PD and leads to a lower POPF incidence rate.

关键词： Pancreaticoduodenectomy Pancreaticojejunostomy Postoperative pancreatic fistula