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Melittin analog p5RHH enhances recombinant adeno-associated virus transduction efficiency

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Journal of Integrative Medicine 2024年第1期22卷 72-82页

作者： Jing-shun Meng Yun he heng-bin Yang Li-ping Zhou Si-yuan Wang Xi-lin Feng Omar Yahya Al-shargi Xiao-min Yu Li-qing Zhu Chang-quan Ling Shanghai University of Traditional Chinese Medicine Shanghai 201203China Oncology Department of Traditional Chinese Medicine Changhai HospitalNaval Medical UniversityShanghai 200433China Faculty of Traditional Chinese Medicine Naval Medical UniversityShanghai 200043China State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology(Ministry of Education) School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Yantai Fuheng Biological Technology Co. Ltd.YantaiShandong Province 264006China. Department of Clinical Laboratory the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina

Objective Melittin and its derivatives have been characterized to establish effective gene delivery systems.Their capability of facilitating endosomal release enhances the nanoparticles-based gene delivery.Nevertheless,little investigation has been conducted to explore its potential application in the context of viral vectors.Methods Various melittin-derived peptides were inserted into the loop VIII of the capsid proteins of recombinant adeno-associated virus vectors.These vectors carrying either gfp or fluc genes were subjected to qPCR assays and transduction assays of heK293T cells to investigate the efficiency of vector production and gene delivery.In addition,the ability of a specific p5RHH-rAAV vector to deliver genes was examined through in vitro transduction of different cultured cells and in vivo tail vein administration to C57BL/6 mice.Finally,the intricate details of the vector-mediated transduction mechanisms were revealed by specific pharmacological inhibitors of every stage of the rAAV2 intracellular life cycle.Results A total of 76 melittin-related peptides were compiled from existing literature.Among them,cMA2,Melt13,p5RHH and aAR3 were found to significantly enhance the gene delivery efficiency of rAAV2 vectors.The p5RHH-rAAV2 vectors efficiently transduced not only rAAV-potent cell lines but also cell lines previously considered resistant to rAAV.Mechanistically,bafilomycin A1,a vacuolar endosome acidification inhibitor,completely inhibited the transgene expression mediated by the p5RHH-rAAV2 vectors.Most importantly,p5RHH-rAAV8 vectors also demonstrated increased hepatic transduction in vivo in C57BL/6 mice.Conclusion The incorporation of melittin analogues into the rAAV capsids results in a significant improvement in rAAV-mediated transgene expression.While further modifications remain an area of interest,our studies have substantially broadened the pharmacological prospects of melittin in the context of viral vector-mediated gene delivery.

关键词： Melittin Recombinant adeno-associated virus Capsid engineering Transduction efficiency

Strong shock propagation for the finite-source circular blast in a confined domain

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Applied Mathematics and Mechanics(English Edition) 2024年第6期45卷 1071-1084页

作者： Qihang MA Kaileong CHONG Bofu WANG quan ZHOU Shanghai Key Laboratory of Mechanics in Energy Engineering Shanghai Institute of Applied Mathematics and MechanicsSchool of Mechanics and Engineering ScienceShanghai UniversityShanghai 200072China Shanghai Institute of Aircraft Mechanics and Control Shanghai 200092China

The circular explosion wave produced by the abrupt discharge of gas from a high-temperature heat source serves as a crucial model for addressing explosion phenomena in compressible flow.The reflection of the primary shock and its propagation within a confined domain are studied both theoretically and numerically in this research.Under the assumption of strong shock,the scaling law governing propagation of the main shock is proposed.The dimensionless frequency of reflected shock propagation is associated with the confined distance.The numerical simulation for the circular explosion problem in a confined domain is performed for validation.Under the influence of confinement,the principal shock wave systematically undergoes reflection within the domain until it weakens,leading to the non-monotonic attenuation of kinetic energy in the explosion fireball and periodic oscillations of the fireball volume with a certain frequency.The simulation results indicate that the frequency of kinetic energy attenuation and the volume oscillation of the explosive fireball align consistently with the scaling law.

关键词： explosion confinement main shock frequency

The breaking of spin symmetry in the single-particle resonances in deformed nuclei

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Nuclear Science and Techniques 2024年第3期35卷 237-245页

作者： Zhen-Yu Zheng Shou-Wan Chen quan Liu School of Physics and Optoelectronic Engineering Anhui UniversityHefei 230601China

The exploration of spin symmetry (SS) in nuclear physics has been instrumental in identifying atomic nucleus structures.In this study,we solve the Dirac equation from the relativistic mean field (RMF) in complex momentum representation.We investigated SS and its breaking in single-particle resonant states within deformed nuclei,with a focus on the illustrative nucleus168Er.This was the initial discovery of a resonant spin doublet in a deformed nucleus,with the expectation of the SS approaching the continuum threshold.With increasing single-particle energy,the splitting of the resonant spin doublets widened significantly.This escalating splitting implies diminishing adherence to the SS,indicating a departure from the expected behavior as the energy levels increase.We also analyzed the width of the resonant states,showing that lower orbital angular momentum resonances possess shorter decay times and that SS is preserved within broad resonant doublets,as opposed to narrow resonant doublets.Comparing the radial density of the upper components for the bound-state and resonant-state doublets,it becomes evident that while SS is well-preserved in the bound states,it deteriorates in the resonant states.The impact of nuclear deformation (β_(2)) on SS was examined,demonstrating that an increase in β_(2) resulted in higher energy and width splitting in the resonant spin doublets,which is attributed to increased component mixing.Furthermore,the sensitivity of spin doublets to various potential parameters such as surface diffuseness (a),radius (R),and depth (Σ0) is discussed,emphasizing the role of these parameters in SS.This study provides valuable insights into the behavior of spin doublets in deformed nuclei and their interplay with the nuclear structure,thereby advancing our understanding of SS in the resonance state.

关键词： Spin symmetry Resonant states Deformed nuclei Complex-momentum representation

KnowledgeMapping of Hybrid Solar PV andWind Energy Standalone Systems: A Bibliometric Analysis

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Energy Engineering 2024年第7期121卷 1781-1803页

作者： quan Zhou Haiyang Li School of Mechanical and Electrical Engineering Central South University of Forestry and TechnologyChangsha410004China

Renewable energy is becoming more attractive as traditional fossil fuels are rapidly depleted and expensive,and their use would release pollutants.Power systems that use both wind and solar energy are more reliable and efficient than those that utilize only one energy.Hybrid renewable energy systems(HRES)are viable for remote areas operating in standalone mode.This paper aims to present the state-of-the-art research on off-grid solar-wind hybrid energy systems over the last two decades.More than 1500 published articles extracted from the Web of Science are analyzed by bibliometric methods and processed by CiteSpace to present the results with figures and tables.Productive countries and highly cited authors are identified,and hot topics with hotspot articles are shown in landscape and timeline views.Emerging trends and new developments related to techno-economic analysis and microgrids,as well as the application of HOMER software,are predicted based on the analysis of citation bursts.Furthermore,the opportunities of hybrid energy systems for sustainable development are discussed,and challenges and possible solutions are proposed.The study of this paper provides researchers with a comprehensive understanding and intuitive representation of standalone solar-wind hybrid energy systems.

关键词： Wind energy solar PV standalone citation bursts emerging trends

Weak type(1, 1) of the Riesz transform on some direct product manifolds with exponential volume growth

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Science China Mathematics 2024年第7期67卷 1599-1622页

作者： Hong-quan Li Jie-Xiang Zhu School of Mathematical Sciences Fudan UniversityShanghai200433China Center for Applied Mathematics Tianjin UniversityTianjin300072China

In this paper, we are concerned with the Riesz transform on the direct product manifold H^(n)× M,where H^(n) is the n-dimensional real hyperbolic space, and M is a connected complete non-compact Riemannian manifold satisfying the volume doubling property and generalized Gaussian or sub-Gaussian upper estimates for the heat kernel. We establish its weak type(1, 1) property. In addition, we obtain the weak type(1, 1) of the heat maximal operator in the same setting. Our arguments also work for a large class of direct product manifolds with exponential volume growth. Particularly, we provide a simpler proof of weak type(1, 1) boundedness of some operators considered in the work of Li et al.(2016).

关键词： Riesz transform heat kernel direct product real hyperbolic space

Distributed Harmonic Power Sharing with Voltage Distortion Suppression in Islanded Microgrids Considering Non-linear Loads

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CSEE Journal of Power and Energy Systems 2024年第1期10卷 117-128页

作者： Guannan Lou Shanlin Li Wei Gu quan Yang School of Electrical Engineering Southeast UniversityNanjingJiangsu 210096China the Jiangsu Provincial Key Laboratory of Smart Grid Technology and Equipment Southeast University Dongguan Power Supply Bureau of Guangdong Power Grid Company Guangdong 523000China

In contemporary power grids or microgrids,harmonic distortion has emerged as one of the critical power quality issues for utility power grids,which has escalated especially due to the high penetration of power-electronic-converter-interfaced distributed generation(DG).This paper first illustrates the prevalent dispute revolving around the harmonic power sharing and distortion restraint,and subsequently proposes a consensusbased framework that facilitates an accurate sharing of harmonics among multi-DGs connected in parallel,with an effective suppression of the output voltage distortion.Compared with the majority of existing studies addressing the issue of voltage harmonics at the point of common coupling(PCC),our method primarily emphasizes on the output voltage distortion since the power quality requirement for certain local critical loads is often known to be high.With the help of adaptive regulation,the overall distortion produced at the output terminals of DGs can be retained within an acceptable range.The working principle of the proposed control method,which is not only easy to implement but also independent of model parameters,is further described in detail.Employing the small-signal dynamic model,the system stability and robustness are analyzed.The hardware-in-the-loop(HIL)simulations aid in determining the outcome of the proposed strategy in microgrid control.

关键词： Distributed generation droop control harmonic power sharing microgrid voltage distortion mitigation

Delayed neurological dysfunction following posterior laminectomy with lateral mass screw fixation:A case report and review of literature

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World Journal of Clinical Cases 2024年第7期12卷 1356-1364页

作者： Rui-Zhong Yan Chao Chen Chu-Ran Lin Yan-Hui Wei Zhi-Jian Guo Ya-Kun Li quan Zhang he-Yi Shen Hao-Lin Sun Department of Orthopedics Taiyuan Central HospitalTaiyuan 030009Shanxi ProvinceChina Department of Orthopedics Graduate School Shanxi University of Traditional Chinese MedicineTaiyuan 030024Shanxi ProvinceChina Department of Orthopedics Peking University First HospitalBeijing 100034China

BACKGROUND While most complications of cervical surgery are reversible,some,such as symptomatic postoperative spinal epidural hematoma(SEH),which generally occurs within 24 h,are associated with increased morbidity and mortality.Delayed neurological dysfunction is diagnosed in cases when symptoms present>3 d postoperatively.Owing to its rarity,the risk factors for delayed neurological dysfunction are unclear.Consequently,this condition can result in irreversible neurological deficits and serious consequences.In this paper,we present a case of postoperative SEH that developed three days after hematoma evacuation.CASE SUMMARY A 68-year-old man with an American Spinal Injury Association(ASIA)grade C injury was admitted to our hospital with neck pain and tetraplegia following a fall.The C3-C7 posterior laminectomy and the lateral mass screw fixation surgery were performed on the tenth day.Postoperatively,the patient showed no changes in muscle strength or ASIA grade.The patient experienced neck pain and subcutaneous swelling on the third day postoperatively,his muscle strength decreased,and his ASIA score was grade A.Magnetic resonance imaging showed hypointense signals on T1 weighted image(T1WI)and T2WI located behind the epidural space,with spinal cord compression.Emergency surgical intervention for the hematoma was performed 12 h after onset.Although hypoproteinemia and pleural effusion did not improve in the perioperative period,the patient recovered to ASIA grade C on day 30 after surgery,and was transferred to a functional rehabilitation exercise unit.CONCLUSION This case shows that amelioration of low blood albumin and pleural effusion is an important aspect of the perioperative management of cervical surgery.Surgery to relieve the pressure on the spinal cord should be performed as soon as possible to decrease neurological disabilities.

关键词： Delayed postoperative spinal epidural hematoma Complications Spinal surgery Hypoproteinemia Risk factor Case report

ROS Balance Autoregulating Core-Shell CeO_(2)@ZIF-8/Au Nanoplatform for Wound Repair

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Nano-Micro Letters 2024年第8期16卷 341-361页

作者： Xi Zhou quan Zhou Zhaozhi he Yi Xiao Yan Liu Zhuohang Huang Yaoji Sun Jiawei Wang Zhengdong Zhao Xiaozhou Liu Bin Zhou Lei Ren Yu Sun Zhiwei Chen Xingcai Zhang The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province Research Center of Biomedical Engineering of XiamenDepartment of BiomaterialsCollege of MaterialsXiamen UniversityXiamen 361005People’s Republic of China Department of Electronic Science Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance ResearchSchool of Electronic Science and EngineeringXiamen UniversityXiamen 361005People’s Republic of China John A Paulson School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA Department of Otorhinolaryngology Union Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022People’s Republic of China NO.1 Middle School Affiliated to Central China Normal University Wuhan 430223People’s Republic of China

Reactive oxygen species(ROS)plays important roles in living organisms.While ROS is a double-edged sword,which can eliminate drug-resistant bacteria,but excessive levels can cause oxidative damage to cells.A core–shell nanozyme,Ce O_(2)@ZIF-8/Au,has been crafted,spontaneously activating both ROS generating and scavenging functions,achieving the multifaceted functions of eliminating bacteria,reducing inflammation,and promoting wound healing.The Au Nanoparticles(NPs)on the shell exhibit high-efficiency peroxidase-like activity,producing ROS to kill bacteria.Meanwhile,the encapsulation of Ce O_(2) core within ZIF-8 provides a seal for temporarily limiting the superoxide dismutase and catalase-like activities of Ce O_(2) nanoparticles.Subsequently,as the ZIF-8 structure decomposes in the acidic microenvironment,the Ce O_(2) core is gradually released,exerting its ROS scavenging activity to eliminate excess ROS produced by the Au NPs.These two functions automatically and continuously regulate the balance of ROS levels,ultimately achieving the function of killing bacteria,reducing inflammation,and promoting wound healing.Such innovative ROS spontaneous regulators hold immense potential for revolutionizing the field of antibacterial agents and therapies.

关键词： Metal-organic framework(MOF) Reactive oxygen species(ROS) Cerium dioxide Au nanoparticles Wound healing

Explosion of research on psychopathology and social media use after COVID-19:A scientometric study

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World Journal of Psychiatry 2024年第5期14卷 742-759页

作者： Meng-Di Zhang Rong-quan he Jia-Yuan Luo Wan-Ying Huang Jing-Yu Wei Jian Dai Hong Huang Zhen Yang Jin-Liang Kong Gang Chen Department of Pathology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Medical Oncology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Clinical Psychology Jiangbin Hospital of Guangxi Zhuang Autonomous RegionNanning 530021Guangxi Zhuang Autonomous RegionChina Division of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND Despite advances in research on psychopathology and social media use,no comprehensive review has examined published papers on this type of research and considered how it was affected by the coronavirus disease 2019(COVID-19)outbreak.AIM To explore the status of research on psychopathology and social media use before and after the COVID-19 outbreak.METHODS We used Bibliometrix(an R software package)to conduct a scientometric analysis of 4588 relevant studies drawn from the Web of Science Core Collection,PubMed,and Scopus databases.RESULTS Such research output was scarce before COVID-19,but exploded after the pandemic with the publication of a number of high-impact articles.Key authors and institutions,located primarily in developed countries,maintained their core positions,largely uninfluenced by COVID-19;however,research production and collaboration in developing countries increased significantly after COVID-19.Through the analysis of keywords,we identified commonly used methods in this field,together with specific populations,psychopathological conditions,and clinical treatments.Researchers have devoted increasing attention to gender differences in psychopathological states and linked COVID-19 strongly to depression,with depression detection becoming a new trend.Developments in research on psychopathology and social media use are unbalanced and uncoordinated across countries/regions,and more indepth clinical studies should be conducted in the future.CONCLUSION After COVID-19,there was an increased level of concern about mental health issues and a changing emphasis on social media use and the impact of public health emergencies.

关键词： Psychopathology Social media Bibliometrics Web of Science PubMed Scopus