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Ginsenoside Rh1 regulates the immune microenvironment of hepatocellular carcinoma via the glucocorticoid receptor

Journal of Integrative Medicine 2024年第6期22卷 709-718页

作者： Xiong-hui Wang Ya-lan Fu Yan-nan Xu Peng-cheng Zhang Tian-xiao Zheng chang-quan Ling Ying-lu Feng Department of Traditional Chinese Medicine First Affiliated Hospital of Naval Medical UniversityShanghai 200433China PLA Joint Logistics Support Force No.967 Hospital Dalian 116021Liaoning ProvinceChina Department of Integrated Traditional Chinese and Western Medicine Medical College of Qingdao UniversityQingdao 266071Shandong ProvinceChina PLA Joint Logistics Support Force No.920 Hospital Kunming 650100Yunnan ProvinceChina First Affiliated Hospital of Zhejiang Chinese Medical University(Zhejiang Provincial Hospital of Chinese Medicine) Zhejiang Chinese Medical UniversityHangzhou 310053Zhejiang ProvinceChina Department of Traditional Chinese Medicine PLA Navy No.971 HospitalQingdao 266071Shandong ProvinceChina

Objective:Ginsenoside Rh1(G-Rh1)has been confirmed to inhibit the growth of breast cancer and colon cancer,but its therapeutic effect on hepatocellular carcinoma(HCC)is *** study investigates the therapeutic effect of G-Rh1 on HCC as well as the underlying ***:Bioinformatics methods were used to analyze glucocorticoid receptor(GR)expression and the tumor microenvironment in HCC tissues from HCC *** effect of G-Rh1 on HCC cells was investigated in vitro using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide *** therapeutic effect of G-Rh1 was investigated in vivo using subcutaneous transplantation models in C57BL/6J and nude ***,the proportion of infiltrating immune cells in tumors was analyzed using flow cytometry,the GR and major histocompatibility complex class-I(MHC-I)expression of HCC cells after G-Rh1 treatment was analyzed using Western blotting,and G-Rh1-treated Hepa1-6 cells were cocultured with bone marrow-derived dendritic cells and B3Z T cells to further analyze the ability of G-Rh1 to induce dendritic cell(DC)maturation and CD8+T cell ***:GR expression was upregulated in HCC tissues,and high GR expression was associated with a worsened immune *** vitro studies showed that G-Rh1 had no significant effect on the proliferation of HCC cells,while in vivo studies showed that G-Rh1 exerted antitumor effects in C57BL/6J mice but not in nude *** research revealed that G-Rh1 ameliorated the immunosuppressive tumor microenvironment,thereby enhancing the antitumor effects of lenvatinib by increasing the infiltration of CD8+T cells,mature DCs,and MHC-I-positive ***-I was upregulated by G-Rh1 via GR ***,overexpression of GR abolished the G-Rh1-mediated promotion of MHC-I expression in Huh7 cells,as well as the maturation of DCs and the activation of CD8+T ***:G-Rh1 can regulate the immune microenvironment of HCC by targeting

关键词： Ginsenoside Rh1 Glucocorticoid receptor MHC-I Immune microenvironment Hepatocellular carcinoma

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Oral attenuated Salmonella typhimurium vaccine against MG7-Ag mimotope of gastric cancer

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World Journal of Gastroenterology 2005年第12期11卷 1833-1836页

作者： Fan-PingMeng JieDing Zhao-CaiYu quan-LiHan chang-CunGuo NaLiu Dai-MingFan InstituteofDigestiveDisease XijingHospitalFourthMilitaryMedicalUniversityXi'an710032ShaanxiProvinceChina

AIM: To develop an oral attenuated Salmonella typhimurium vaccine against gastric cancer and to evaluate its efficacy in mice. METHODS: A complementary sequence of Nco Ⅰ site and a sequence coding for MG7-Ag mimotope were designed at the 5'terminus of forward primer. Using p1.2 Ⅱ-HBCAg plasmid as template, PCR was performed to get a fusion gene of the mimotope and a HBcAg gene. The fusion gene was then subcloned into the plasmid pYA3341 complementary to Salmonella typhimurium X4550, and the recombinant plasmid was then transformed into attenuated Salmonella typhimurium X4550. Balb/c mice were orally immunized with the recombinant Salmonella typhimurium X4550. The mice were immunized every 2 wk to reinforce the immunity. At the 6th wk, serum titer of antibody was detected by ELISA, and at the 8th wk, cellular immunity was detected by 51Cr release test. Ehrlich ascites carcinoma cells expressing MG7-Ag were used in tumor challenge assay as a model to evaluate the protective effect of the vaccine. RESULTS: Serum titer of antibody against MG7-Ag was significantly higher in mice immunized with the vaccine than in control groups (0.9538±0.043 vs 0.6531±0.018, P<0.01; 0.9538±0.043 vs 0.6915±0.012, P<0.01), while in vitro 51Cr release assay of the splenocytes showed no statistical difference in the three groups. Two weeks after tumor challenge, 1 in 5 immunized mice was tumor free, while all the mice in the control group presented tumor. CONCLUSION: Oral attenuated Salmonella typhimurium vaccine against the MG7-Ag mimotope of gastric cancer is immunogenic. It can induce significant humoral immunity against tumors in mice, and has some protective effects.

关键词： Salmonella typhimuriunr Gastric cancer

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中西医结合治疗原发性肝癌系列临床方案创建的几点体会

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中国中西医结合杂志 2019年第10期39卷 1166-1168页

作者：凌昌全海军军医大学附属长海医院中医肿瘤科

中医药是我国原发性肝癌(以下简称肝癌)防治体系的重要组成部分,也是区别于其他国家地区体系的重要特色和优势所在。既往的大量临床实践中,中医药治疗肝癌因其独特的理念方法,得到了极为广泛的应用,取得了令人瞩目的成绩。近年来中医药... 详细信息

中医药是我国原发性肝癌(以下简称肝癌)防治体系的重要组成部分,也是区别于其他国家地区体系的重要特色和优势所在。既往的大量临床实践中,中医药治疗肝癌因其独特的理念方法,得到了极为广泛的应用,取得了令人瞩目的成绩。近年来中医药治疗肝癌的临床研究日益增多,但目前此类研究仍以临床观察或病例报道为主,大多停留于经验水平,缺乏有力的循证医学证据,更缺乏经系统总结较为规范的治疗方案。

关键词：原发性肝癌中西医结合治疗临床方案中医药治疗循证医学证据临床实践临床研究病例报道

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作战系统互操作应用效能的综合评估方法

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系统工程与电子技术 2007年第1期29卷 73-77页

作者：金培权安昌全岳丽华中国科学技术大学计算机系安徽合肥230027

给出了互操作及互操作应用效能评估的定义,针对效能评估一般模式的不足,综合主成份分析法理论和层次化思想,建立了作战系统互操作的应用效能评估指标体系,提出运用多种方法的综合评估方法。仿真实例的评估及分析表明,所建立的指标体系... 详细信息

给出了互操作及互操作应用效能评估的定义,针对效能评估一般模式的不足,综合主成份分析法理论和层次化思想,建立了作战系统互操作的应用效能评估指标体系,提出运用多种方法的综合评估方法。仿真实例的评估及分析表明,所建立的指标体系和综合评估方法可操作性强,能够满足作战系统互操作应用效能评估的需求。

关键词：作战系统互操作性应用效能理想替代法综合评估

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A 12-year cohort study on the efficacy of plasmaderived hepatitis B vaccine in rural newborns

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World Journal of Gastroenterology 2000年第3期6卷 381-383页

作者： Hong Bin Liu Zong Da Meng Jing Chen Ma chang quan Han Ying Lin Zhang Zhan Chun Xing Yu Wei Zhang Yu Zhong Liu Hui Lin Cao Virology Department of Hebei Provincial Sanitary and Anti-Epidemic Station Baoding 071000Hebei ProvinceChina Department of Hepatitis Zhengding county Sanitary and Anti-Epidemic StationZhengding 070800ShijiazhuangHebei ProvinceChina Department of Hepatitis Institute of VirologyChinese Academy of Preventive MedicineBeijing 100052China

AIM To understand the anti-HBs persistenceand the long-term preventive efficacy in ruralnewborns after vaccination with plasma-derivedhepatitis B *** In the time of expanded program onimmunization(EPI),the newborns werevaccinated with 10μg×3 doses of hepatitis Bvaccine and 762 newborns who were HBsAgnegative after primary immunization wereselected for cohort observation from 1986 *** serum samples were detectedqualitatively and quantitatively for hepatitis Binfecting markers,including HBsAg,anti-HBsand anti-HBc by SPRIA *** annual HBsAgpositive conversion rate was counted by life-table ***①The anti-HBs positive rate was94.44% for the babies born to HBsAg negativemothers and 84.21% for those born to HBsAgpositive mothers in the 1st year afterimmunization,and dropped to 51.31% and52.50% in the 12th year *** valuewas dropped from 31.62 to 3.13 and 23.99 to 3.65in the 2nd to the 12th year *** a marked drop in GMT at the 3rd to the 5thyear,and in anti-HBs positive rate at the 9th tothe 10th year.②In the period of 12 yearsobservation,the person-year HBsAg positive conversion rates were 0.12%(5/4150.0)innewborns born to HBsAg negative mothers and0.20%(1/508.0)in those born to HBsAgpositive mothers,and none of the HBsAgpositive converted children became HBsAgchronic *** with the baselinebefore immunization,the protective rates were97.19% and 95.32% *** The protective efficacy ofplasma-derived hepatitis B vaccine persisted atleast 12 years,and a booster dose seems notnecessary within at least 12 years after theprimary three-doses immunization to newbornsborn to HBsAg negative mothers.

关键词： hepatitis B vaccine HBsAg vaccination rural newborn cohort study

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Melittin analog p5RHH enhances recombinant adeno-associated virus transduction efficiency

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Journal of Integrative Medicine 2024年第1期22卷 72-82页

作者： Jing-shun Meng Yun He Heng-bin Yang Li-ping Zhou Si-yuan Wang Xi-lin Feng Omar Yahya Al-shargi Xiao-min Yu Li-qing Zhu chang-quan Ling Shanghai University of Traditional Chinese Medicine Shanghai 201203China Oncology Department of Traditional Chinese Medicine Changhai HospitalNaval Medical UniversityShanghai 200433China Faculty of Traditional Chinese Medicine Naval Medical UniversityShanghai 200043China State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology(Ministry of Education) School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Yantai Fuheng Biological Technology Co. Ltd.YantaiShandong Province 264006China. Department of Clinical Laboratory the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina

Objective Melittin and its derivatives have been characterized to establish effective gene delivery *** capability of facilitating endosomal release enhances the nanoparticles-based gene ***,little investigation has been conducted to explore its potential application in the context of viral *** Various melittin-derived peptides were inserted into the loop VIII of the capsid proteins of recombinant adeno-associated virus *** vectors carrying either gfp or fluc genes were subjected to qPCR assays and transduction assays of HEK293T cells to investigate the efficiency of vector production and gene *** addition,the ability of a specific p5RHH-rAAV vector to deliver genes was examined through in vitro transduction of different cultured cells and in vivo tail vein administration to C57BL/6 ***,the intricate details of the vector-mediated transduction mechanisms were revealed by specific pharmacological inhibitors of every stage of the rAAV2 intracellular life *** A total of 76 melittin-related peptides were compiled from existing *** them,cMA2,Melt13,p5RHH and aAR3 were found to significantly enhance the gene delivery efficiency of rAAV2 *** p5RHH-rAAV2 vectors efficiently transduced not only rAAV-potent cell lines but also cell lines previously considered resistant to ***,bafilomycin A1,a vacuolar endosome acidification inhibitor,completely inhibited the transgene expression mediated by the p5RHH-rAAV2 *** importantly,p5RHH-rAAV8 vectors also demonstrated increased hepatic transduction in vivo in C57BL/6 *** The incorporation of melittin analogues into the rAAV capsids results in a significant improvement in rAAV-mediated transgene *** further modifications remain an area of interest,our studies have substantially broadened the pharmacological prospects of melittin in the context of viral vector-mediated gene delivery.

关键词： Melittin Recombinant adeno-associated virus Capsid engineering Transduction efficiency

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MXene Hybridized Polymer with Enhanced Electromagnetic Energy Harvest for Sensitized Microwave Actuation and Self‑Powered Motion Sensing

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Nano-Micro Letters 2025年第3期17卷 265-279页

作者： Yu‑Ze Wang Yu‑chang Wang Ting‑Ting Liu quan‑Liang Zhao Chen‑Sha Li Mao‑Sheng Cao School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081People’s Republic of China School of Materials Science and Engineering Peking UniversityBeijing 100871People’s Republic of China School of Mechanical and Material Engineering North China University of TechnologyBeijing 100144People’s Republic of China Key Laboratory of Functional Inorganic Material Chemistry Ministry of Education of the People’s Republic of ChinaHeilongjiang UniversityHarbin 150080People’s Republic of China

Polymeric microwave actuators combining tissue-like softness with programmablemicrowave-responsive deformation hold great promise for mobile intelligentdevices and bionic soft robots. However, their application is challenged by restricted electromagneticsensitivity and intricate sensing coupling. In this study, a sensitized polymericmicrowave actuator is fabricated by hybridizing a liquid crystal polymer with Ti3C2Tx(MXene). Compared to the initial counterpart, the hybrid polymer exhibits unique spacechargepolarization and interfacial polarization, resulting in significant improvements of230% in the dielectric loss factor and 830% in the apparent efficiency of electromagneticenergy harvest. The sensitized microwave actuation demonstrates as the shortenedresponse time of nearly 10 s, which is merely 13% of that for the initial shape memory polymer. Moreover, the ultra-low content of MXene (upto 0.15 wt%) benefits for maintaining the actuation potential of the hybrid polymer. An innovative self-powered sensing prototype that combinesdriving and piezoelectric polymers is developed, which generates real-time electric potential feedback (open-circuit potential of ~ 3 mV) duringactuation. The polarization-dominant energy conversion mechanism observed in the MXene-polymer hybrid structure furnishes a new approachfor developing efficient electromagnetic dissipative structures and shows potential for advancing polymeric electromagnetic intelligent devices.

关键词： Microwave absorption Electromagnetic response Energy harvest Self-sensing Soft actuator

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First record of disk-footed bat Eudiscopus denticulus(Chiroptera,Vespertilionidae)from China and resolution of phylogenetic position of the genus

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Zoological Research 2021年第1期42卷 94-99页

作者： Wen-Hua Yu Gabor Csorba Zheng-Lan-Yi Huang Yan-Nan Li Shuo Liu Rui-chang quan Qiao-Yan Wang Hong-Yan Shi Yi Wu Song Li Key Laboratory of Conservation and Application in Biodiversity of South China School of Life SciencesGuangzhou UniversityGuangzhouGuangdong 510006China Department of Zoology Hungarian Natural History MuseumBudapest H-1088Hungary Kunming Natural History Museum of Zoology Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Center for Integrative Conservation Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglaYunnan 666303China Research Institute of Xishuangbanna National Nature Reserve JinghongYunnan 666100China Ecological Security and Protection Key Laboratory of Sichuan Province Mianyang Teacher’s CollegeMianyangSichuan 621000China

DEAR EDITOR,The disk-footed bat Eudiscopus denticulus(Osgood,1932)is a rare species in Southeast *** two chiropteran surveys in the summer of 1981 and 2019,eight and three small Myotis-like bats with distinct disk-like hindfeet were collected from Yunnan Province,China,***,craniodental,and phylogenetic evidence confirmed these specimens as ***,representing a new genus in *** complete mitochondrial genome consistently showed robust support for *** as a basal lineage within *** coding patterns and characteristics of its mitochondrial genome were similar to that of other published genomes from *** echolocation signals of the newly collected individuals were *** potential distribution range of Eudiscopus in Southeast Asia inferred using the MaxEnt model indicated its potential occurrence along the southern border region of Yunnan,China.

关键词： China. record representing

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Clinical practice guidelines for the treatment of primary liver cancer with integrative traditional Chinese and Western medicine

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Journal of Integrative Medicine 2018年第4期16卷 236-248页

作者： chang-quan Ling Jia Fan Hong-sheng Lin Feng Shen Zhen-ye Xu Li-zhu Lin Shu-kui Qin Wei-ping Zhou Xiao-feng Zhai Bai Li Qing-hui Zhou Department of Integrative Oncology Changhai Hospital of Traditional Chinese MedicineSecond Military Medical UniversityShanghai 200433China Department of Liver Surgery and Transplantation Liver Cancer InstituteZhongshan HospitalFudan UniversityShanghai 200032China Department of Oncology Guang'anmen HospitalChina Academy of Chinese Medical SciencesBeijing 100053China Department of Hepatobiliary Surgery Eastern Hepatabiliary Surgery HospitalSecond Military Medical UniversityShanghai 200433China Department of Oncology Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Department of Oncology First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510405China Cancer Center of Bayi Hospital Nanjing University of Chinese Medicine210002China Department of Rehabilitative Medicine Changhai Hospital of Traditional Chinese MedicineSecond Military Medical UniversityShanghai 200433China Department of Acupuncture-Moxihustion and Tuina Changhai Hospital of Traditional Chinese MedicineSecond Military Medical UniversityShanghai 200433China

Traditional Chinese medicine （TCM） is an important part of the treatment of primary liver cancer （PLC） in China; however, the current instructions for the integrative use of traditional Chinese and Western medicine for PLC are mostly based on expert opinion. There is no evidence-based guideline for clinical prac-tice in this field. Therefore, the Shanghai Association of Chinese Integrative Medicine has established a multidisciplinary working group to develop this guideline, which focuses on the most important questions about the use of TCM during PLC treatment. This guideline was developed following the method- ological process recommended by the World Health Organization Handbook for Guideline Development. Two rounds of questionnaire survey were performed to identify clinical questions; published evidence was searched; the Grading of Recommendations Assessment, Development and Evaluation approach was used to evaluate the body of evidence; and recommendations were formulated by combining the quality of evidence, patient preferences and values, and other risk factors. The guideline was written based on the Reporting Items for Practice Guidelines in Healthcare tool. This guideline contains 10 recommendations related to 8 questions, including recommendations for early treatment by TCM after surgery, TCM combined with transcatheter arterial chemoembolization for advanced PLC, TCM drugs for external use, and acupuncture and moxibustion therapy.

关键词： Primary liver cancerClinical practice guidelines Traditional Chinese medicineIntegrative medicine

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外溢流型碳化尾气净氨塔设计

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石油化工设备 2000年第5期29卷 36-37页

作者：李全昶唐山三友集团碱业有限公司河北丰南063305

符　号　说　明　　　 X1 ——进液中氨的摩尔比率HT——板间距 ,mX2 ——出液中氨的摩尔比率wm——空塔最大气速 ,m /sY1 ——进气中氨的摩尔比率w0 ——实际最大气速 ,m /sY2 ——出气中氨的摩尔比率d——泡罩直径 ,mY1 ——进液圈液... 详细信息

符　号　说　明　　　 X1 ——进液中氨的摩尔比率HT——板间距 ,mX2 ——出液中氨的摩尔比率wm——空塔最大气速 ,m /sY1 ——进气中氨的摩尔比率w0 ——实际最大气速 ,m /sY2 ——出气中氨的摩尔比率d——泡罩直径 ,mY1 ——进液圈液体上面氨平衡的摩尔比率VG/L——鼓泡线气流比 ,m3/m· sY2 ——出液圈液体上面氨平衡的摩尔比率h——全塔阻力降 ,kg/m2VG——气相负荷 ,m3/sh1 ——单板阻力降 ,kg/m2　　　ρG——气相密度 ,kg/m3ΔP1 ——单板鼓泡流体阻力 ,kg/m2VL——液相负荷 ,m3/sΔP2 ——气囱、菌帽的流体阻力 ,kg/m2ρL——液相密度 ,kg/m3a——鼓泡深度 ,mn——理论塔板数b——齿跟下气流深度 ,mη——塔板效率λ——阻力系数N——实际塔板数u——气囱中尾气流速 ,m/sDT——塔径 ,mH1 ——液封高度 ,m1　综述碳化尾气净氨塔是纯碱行业中回收碳化 (清洗 )尾气中氨的主要设备 (吸收介质大多为精制盐水 ) ,以前采用的是内溢流型塔。后改为外溢流型塔 ,两种塔型的主要区别是塔板降液管不同 ,见图 1。图 1　...

关键词：设计纯碱设备外溢流型碳化尾气净氨塔

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