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Effect of emulsified isoflurane on apoptosis of anoxia-reoxygenation neonatal rat cardiomyocytes

Asian Pacific Journal of Tropical Medicine 2013年第12期6卷 977-981页

作者： Xiao Liu qu-lian guo Zhong Zhang Long Long Yang Yang Department of Anesthesia Xiangya Hospital Central-South University Department of Cardiothoracic Surgery Xiangya Hospital Central-South University

Objective:To explore the effect of emulsified isoflurane(EI)on apoptosis of anoxia-reoxygenation neonatal rat cardiomyocytea and relevant protein ***:Cardiac muscle anoxiareoxygenation damage model was established with culture in vitro neonatal rat *** cardiomyocytes were divided into control group,model group,fat emulsion group and EI *** cardiomyocytes apoptosis rates and lactic dehydrogenase(LDH),superoxide dismutase(SOD)and malondialdehyde(MDA)index standardization were detected after relevant treatment The expression of apoptosis-related proteins Bel-2,Bax and Caspase-3 were detected with Western blot ***:After hypoxia/reoxygenation(H/R)model was treated by EI,the cells apoptosis rate decreased and was dramatically below the fat emulsion group(P<0.05),Cardiomyocytes biochemical index detection presented that,compared with the control group that the LDH activity and MDA content dramatically increased(P<0.05),while the SOD activity notably decreased(P<0.05);compared with the H/R group,the SOD activity of the fat emulsion group and EI group increased(P<0.05);while the LDH activity and MDA content decreased(P<0.05).And the change of the EI group was more remarkable than the fat emulsion group(P<0.05).The Western blot analysis presented that,compared with the control group,the Bcl-2 protein expression of the other groups significantly decreased(P<0.05),the expressions of Bax protein and Caspase-3protein increased significantly(P<0.05);compared with H/R group,cardiomyocytes Bc1-2protein expression of EI group increased significantly(P<0.05),the expressions of Bax protein and Caspase-3 protein decreased significantly(P<0.05),and the change of EI group was more remarkable than the fat emulsion group(P<0.05).Conclusions:EI can inhabit the apoptosis of anoxia-reoxygenation damage model cardiomyocytes,and may he related to the up-regulation of expression of Bcl-2 and down-regulation of expression of Caspase-3 protein.

关键词： Emulsified isoflurane Apoptosis Anoxia-reoxygenation Neonatal rat Cardiomyocytes

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Rac1 relieves neuronal injury induced by oxygen-glucose deprivation and re-oxygenation via regulation of mitochondrial biogenesis and function

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Neural Regeneration Research 2020年第10期15卷 1937-1946页

作者： Ping-Ping Xia Fan Zhang Cheng Chen Zhi-Hua Wang Na Wang Long-Yan Li qu-lian guo Zhi Ye Department of Anesthesiology Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina National Clinical Research Center for Geriatric Disorders Central South UniversityChangshaHunan ProvinceChina Department of Anesthesiology Hainan General HospitalHaikouHainan ProvinceChina

Certain microRNAs(miRNAs)can function as neuroprotective factors after reperfusion/ischemia brain ***-142-3p can participate in the occurrence and development of tumors and myocardial ischemic injury by negatively regulating the activity of Rac1,but it remains unclear whether miRNA-142-3p also participates in cerebral ischemia/reperfusion *** this study,a model of oxygen-glucose deprivation/re-oxygenation in primary cortical neurons was established and the neurons were transfected with miR-142-3p agomirs or miR-142-3p ***-142-3p expression was down-regulated in neurons when exposed to oxygen-glucose deprivation/***-expression of miR-142-3p using its agomir remarkably promoted cell death and apoptosis induced by oxygen-glucose deprivation/re-oxygenation and improved mitochondrial biogenesis and function,including the expression of peroxisome proliferator-activated receptor-γcoactivator-1α,mitochondrial transcription factor A,and nuclear respiratory factor ***,the opposite effects were produced if miR-142-3p was *** reporter assays verified that Rac Family Small GTPase 1(Rac1)was a target gene of ***-expressed miR-142-3p inhibited NOX2 activity and expression of Rac1 and Rac1-GTPase(its activated form).miR-142-3p antagomirs had opposite effects after oxygen-glucose deprivation/*** results indicate that miR-142-3p down-regulates the expression and activation of Rac1,regulates mitochondrial biogenesis and function,and inhibits oxygen-glucose deprivation damage,thus exerting a neuroprotective *** experiments were approved by the Committee of Experimental Animal Use and Care of Central South University,China(approval No.201703346)on March 7,2017.

关键词： biogenesis ischemia/reperfusion injury microRNAs miR-142-3p mitochondria neuroprotection NOX2 oxygen-glucose deprivation Rac1

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Inhibition of CD38/Cyclic ADP-ribose Pathway Protects Rats against Ropivacaine-induced Convulsion

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Chinese Medical Journal 2017年第19期130卷 2354-2360页

作者： Yu Zou Xin He Qian-Yi Peng qu-lian guo Department of Anesthesiology Xiangya Hospital Central South University Changsha Hunan 410008 China Department of Critical Care Medicine Xiangya Hospital Central South University Changsha Hunan 410008 China

Background： The CD38/cyclic ADP-ribose （cADPR） pathway plays a role in various central nervous system diseases and in morphine tolerance, but its role in local anesthetic intoxication is unknown. The aim of this study was to determine the role of the CD38/cADPR pathway in ropivacaine-induced convulsion. Methods： Forty male Sprague-Dawley rats were randomly divided into five groups （n = 8 per group）： sham group, ropivacaine group, ropivacaine＋8-Br-cADPR （5 nmol） group, ropivacaine＋8-Br-cADPR （10 nmol） group, and ropivacaine＋8-Br-cADPR （20 nmol） group （no rats died）. Rats were intracerebroventricularly injected with normal saline or 8-Br-cADPR 30 min before receiving an intraperitoneal injection of ropivacaine. Electroencephalography and convulsion behavior scores were recorded. The hippocampus was harvested from each group and subjected to nicotinamide adenine dinucleotide and cADPR assays, Western blotting analysis, and malondialdehyde （MDA） and superoxide dismutase （SOD） assays. Results： Intraperitoneal injection of ropivacaine （33.8 mg/kg） induced convulsions in rats. CD38 and cADPR levels increased significantly following ropivacaine-induced convulsion （P = 0.031 and 0.020, respectively, compared with the sham group）. Intraventricular injection of 8-Br-cADPR （5, 10, and 20 nmol） significantly prolonged convulsion latency （P = 0.037, 0.034, and 0.000, respectively）, reduced convulsion duration （P = 0.005, 0.005, and 0.005, respectively）, and reduced convulsion behavior scores （P = 0.015, 0.015, and 0.000, respectively）. Intraventricular injection of 8-Br-cADPR （10 nmol） also increased the B-cell lymphoma-2 （Bcl-2）/Bcl-2-associated X protein ratio （P = 0.044） and reduced cleaved Caspase 3/Caspase 3 ratio, inducible nitric oxide synthase, MDAand SOD levels （P = 0.014, 0.044, 0.001, and 0.010, respectively） compared with the ropivacaine group. Conclusions： The CD38/cADPR pathway is activated in ropivacaine-induced convulsion. Inhibiting this pathway alleviates ropiva

关键词： CD38 Convulsion Cyclic ADP-ribose Nicotinamide Adenine Dinucleotide Ropivacaine

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老年患者腰椎术后应用右美托咪定对舒芬太尼术后镇痛的影响

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中国现代医学杂志 2017年第18期27卷 106-109页

作者：蒋明浩王云姣郭曲练中南大学湘雅医院麻醉科湖南长沙410008

目的观察老年患者腰椎术后应用右美托咪定对舒芬太尼术后镇痛的影响。方法选取2015年1月-2016年1月在该院老年腰椎手术患者90例,随机分为观察组和对照组,每组45例,观察组患者在术后应用(舒芬太尼+右美托咪定)镇痛,对照组患者在术后应用... 详细信息

目的观察老年患者腰椎术后应用右美托咪定对舒芬太尼术后镇痛的影响。方法选取2015年1月-2016年1月在该院老年腰椎手术患者90例,随机分为观察组和对照组,每组45例,观察组患者在术后应用(舒芬太尼+右美托咪定)镇痛,对照组患者在术后应用舒芬太尼镇痛,观察两组患者的镇痛效果和不良反应。结果 (1)观察组在术后多个时间点的视觉模拟评分(VAS)与Ramesay镇静评分均优于对照组,组间差异有统计学意义(P<0.05);(2)观察组在各个时间点的血压、心率及血氧变化均比对照组稳定(P<0.05);(3)观察组不良反应发生率为6.6%,对照组不良反应发生率为37.7%,组间差异有统计学意义(P<0.05)。结论舒芬太尼合用右美托咪定可有效治疗老年患者腰椎术后疼痛,不良反应发生率低。

关键词：老年患者腰椎手术右美托咪定术后镇痛舒芬太尼

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Exosomes:the next-generation therapeutic platform for ischemic stroke

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Neural Regeneration Research 2025年第5期20卷 1221-1235页

作者： Wenjing Yin Hongyin Ma Yang qu Jiaxin Ren Yingying Sun Zhen-Ni guo Yi Yang Stroke Center Department of NeurologyFirst Hospital of Jilin UniversityChangchunJilin ProvinceChina Neuroscience Research Center Department of NeurologyFirst Hospital of Jilin UniversityChangchunJilin ProvinceChina

Current therapeutic strategies for ischemic stroke fall short of the desired objective of neurological functional ***,there is an urgent need to develop new methods for the treatment of this *** are natural cell-derived vesicles that mediate signal transduction between cells under physiological and pathological *** have low immunogenicity,good stability,high delivery efficiency,and the ability to cross the blood–brain *** physiological properties of exosomes have the potential to lead to new breakthroughs in the treatment of ischemic *** rapid development of nanotechnology has advanced the application of engineered exosomes,which can effectively improve targeting ability,enhance therapeutic efficacy,and minimize the dosages *** in technology have also driven clinical translational research on *** this review,we describe the therapeutic effects of exosomes and their positive roles in current treatment strategies for ischemic stroke,including their antiinflammation,anti-apoptosis,autophagy-regulation,angiogenesis,neurogenesis,and glial scar formation reduction ***,it is worth noting that,despite their significant therapeutic potential,there remains a dearth of standardized characterization methods and efficient isolation techniques capable of producing highly purified *** optimization strategies should prioritize the exploration of suitable isolation techniques and the establishment of unified workflows to effectively harness exosomes for diagnostic or therapeutic applications in ischemic ***,our review aims to summarize our understanding of exosome-based treatment prospects in ischemic stroke and foster innovative ideas for the development of exosome-based therapies.

关键词： blood–brain barrier electroacupuncture engineering exercise exosomes ischemic stroke mesenchymal stem cells microglia neuroprotection stents

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Regulation of graphdiyne-based nanozymes with enhanced oxidaselike activity by cobalt and nitrogen codoping

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Nano Research 2025年第1期18卷 21-29页

作者： Jiali Liu Xiaoli Wang Yongfu lian Xue Wang Xiuxiu Wang Xingfa Gao Baoyun Sun Xihong guo CAS Key Laboratory for Biomedical Effects of Nanomaterial&Nanosafety Institute of High Energy PhysicsChinese Academy of ScienceBeijing 100049China Key Laboratory of Functional Inorganic Material Chemistry Ministry of EducationSchool of Chemistry and Materials ScienceHeilongjiang UniversityHarbin 150080China Laboratory of Theoretical and Computational Nanoscience National Center for Nanoscience and TechnologyBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

The development of nanozymes with excellent intrinsic oxidase-like activity and specificity has received increasing ***(GDY)could be a promising choice for designing nanozymes with enhanced oxidase(OXD)-like activity due to its unique structure and ***,Co-N-GDY with high OXD activity but no peroxidase(POD)activity was synthesized by codoping of cobalt(Co)and nitrogen(N)into GDY and compared with other GDY-based nanozymes(including GDY,Co-GDY,and N-GDY).Upon analyzing the doping effect of Co and N on the OXD-like and POD-like activities,we found that the combination of Co and N in GDY played a significant role in enhancing the OXD-like activity,and even reversed the POD-like activity of N-GDY to OXD-like activity of *** electrochemical experiment and the theoretical calculations provided an explanation for the mechanism and showed that the activity was closely linked to the reduction ability of O_(2) or H_(2)O_(2) on the nanozyme substrates,which was determined by the ratedetermining step of the catalytic reaction.

关键词： nanozymes oxidase-like activity graphdiyne heteroatom doping regulation

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钙稳态调节蛋白1在痛性糖尿病神经病变大鼠脊髓背角的表达变化

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中国现代医学杂志 2015年第17期25卷 16-21页

作者：刘文捷郭曲练何万友彭良玉南华大学附属第一医院麻醉科湖南衡阳421001 中南大学湘雅医院麻醉科湖南长沙410008 广东省佛山市第一人民医院麻醉科广东佛山528000

目的研究钙稳态调节蛋白1(CALHM1)在痛性糖尿病神经病变(PDN)大鼠脊髓中的表达。方法构建PDN大鼠模型,检测其行为学改变;利用免疫组织化学法、Western blot及实时荧光定量核酸扩增反应(q RTPCR)检测CALHM1在大鼠脊髓背角神经元上的分布... 详细信息

目的研究钙稳态调节蛋白1(CALHM1)在痛性糖尿病神经病变(PDN)大鼠脊髓中的表达。方法构建PDN大鼠模型,检测其行为学改变;利用免疫组织化学法、Western blot及实时荧光定量核酸扩增反应(q RTPCR)检测CALHM1在大鼠脊髓背角神经元上的分布、表达及m RNA转录;用统计学分析CALHM1 m RNA表达与缩足反应阈值(PWT)的相关性。结果 PDN大鼠的CALHM1及m RNA表达高于对照组,差异有统计学意义,且CALHM1 m RNA表达与50%PWT呈负相关。结论 CALHM1在PDN大鼠脊髓背角神经元中表达增高。

关键词：糖尿病神经病理性疼痛钙稳态调节蛋白1 脊髓背角神经元

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运动饮料及其选用

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解放军体育学院学报 2005年第2期24卷 97-99页

作者：郭杨义屈联国林建棣解放军体育学院广东广州510500

从运动饮料的特点、分类入手,分析了运动饮料的主要成分以及各成分在运动中的作用。

关键词：运动饮料特点分类成分

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Effect of Nb-V microalloying on the hot deformation behavior of medium Mn steels

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 360-368页

作者： Yumeng Wang Qinyi guo Bin Hu Haiwen Luo School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China

The influence of Nb-V microalloying on the hot deformation behavior and microstructures of medium Mn steel(MMS)was investigated by uniaxial hot compression *** establishing the constitutive equations for simulating the measured flow curves,we successfully constructed deformation activation energy(Q)maps and processing maps for identifying the region of flow *** concluded the following consequences of Nb-V alloying for MMS.(i)The critical strain increases and the increment diminishes with the increasing deformation temperature,suggesting that NbC precipitates more efficiently retard dynamic recrystallization(DRX)in MMS compared with solute Nb.(ii)The deformation activation energy of MMS is significantly increased and even higher than that of some reported high Mn steels,suggesting that its ability to retard DRX is greater than that of the high Mn content.(iii)The hot workability of MMS is improved by narrowing the hot processing window for the unstable flow stress,in which fine recrystallized and coarse unrecrystallized grains are present.

关键词： medium manganese steel niobium-vanadium microalloying flow behavior dynamic recrystallization hot workability

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树突状细胞在榄香烯复合瘤苗主动免疫效应中的作用

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上海免疫学杂志 2003年第4期23卷 225-227,231页

作者：屈虹郭连英施广霞李丹朴花沈洁钱振超大连医科大学肿瘤生物治疗研究所大连116027

本实验以HCa F或L6 1 5榄香烯复合瘤苗 (H TCV或L TCV )、H TCV溶解物 (TH )、短小棒状菌 (CP )免疫小鼠 ,分离制备其脾脏DC ,在体外分别用相应瘤细胞溶解物 (H或L )和瘤苗溶解物 (TH或TL )冲激后 ,以MTT法检测其诱导同系小鼠脾不粘附... 详细信息

本实验以HCa F或L6 1 5榄香烯复合瘤苗 (H TCV或L TCV )、H TCV溶解物 (TH )、短小棒状菌 (CP )免疫小鼠 ,分离制备其脾脏DC ,在体外分别用相应瘤细胞溶解物 (H或L )和瘤苗溶解物 (TH或TL )冲激后 ,以MTT法检测其诱导同系小鼠脾不粘附细胞增殖的能力。结果表明用榄香烯复合瘤苗或其溶解物免疫DC供鼠和体外冲激DC ,可增强其诱导同系小鼠脾不粘附细胞增殖的作用。

关键词：树突状细胞榄香烯复合瘤苗抗原递呈

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