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Nano Research 2013年第9期6卷 693-702页

作者： Konggang qu Li Wu jinsong Ren Xiaogang qu Laboratory of Chemical Biology Division of Biological Inorganic Chemistry State Key Laboratory of Rare Earth Resource UtilizationChangchun Institute of Applied Chemistry Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences ChangchunJilin 130022 China

A non-destructive, safe and practical strategy to produce high quality graphene in high yield is urgently required, since this would pave the way for a wide range of applications of graphene in the future. Here we present a pH-responsive water-dispersible method for the exfoliation and functionalization of graphene by using lysozyme. The pH-responsive dispersion of graphene may be useful for the reversible assembly of multicomponent/multifunctional nanohybrid materials and nanoscale electronic devices. More importantly, composites can be easily constructed through the interactions between disulphide groups in lysozyme and gold nanoparticles （AuNPs）. The resulting graphene-AuNPs composites show excellent catalytic activity towards reduction of o-nitroaniline by NaBH4. Since lysozyme is low cost and has antibacterial properties, and has been widely used in food preservation, medicine and the pharmaceutical industry, our approach may open a new scalable route for the manufacture of high-quality, nondestructive graphene for practical applications.

关键词： grapheme exfoliation lysozyme pH-responsive catalysis protein

bZIP Transcription Factor UvATF21 Mediates Vegetative Growth,Conidiation,Stress Tolerance and Is Required for Full Virulence of Rice False Smut Fungus Ustilaginoidea virens

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Rice science 2023年第1期30卷 50-57页

作者： LIU Yueran qu jinsong WANG Yufu YIN Weixiao LUO Chaoxi Hubei Key Laboratory of Plant Pathology/College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China

bZIP proteins are widely distributed in eukaryotic organisms and regulate a diverse range of physiological *** bZIP proteins have previously been identified in Ustilaginoidea ***,the biological roles of these bZIP proteins in this pathogen are still ***,one of these bZIP protein coding genes,UvATF21,was functionally *** deletion of UvATF21resulted in reduced conidiation and pathogenicity despite of the increased vegetative *** deletion mutants also significantly decreased the sensitivity to osmotic and oxidative ***,deletion of UvATF21 exhibited different performances to cell wall integrity *** results indicated that UvATF21 played crucial roles in vegetative growth,conidiation,stress response,and full virulence in ***.

关键词： Ustilaginoidea virens bZIP protein conidiation stress response pathogenicity

Polyoxometalates: metallodrug agents for combating amyloid aggregation

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National Science Review 2024年第7期11卷 279-291页

作者： Mengmeng Ma Zhenqi Liu Huisi Zhao Haochen Zhang jinsong Ren Xiaogang qu Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of Sciences School of Applied Chemistry and Engineering University of Science and Technology of China

Alzheimer's disease(AD) is a devastating neurodegenerative disease that affects ～50 million people globally. The accumulation of amyloid-β(Aβ) plaques, a predominant pathological feature of AD, plays a crucial role in AD pathogenesis. In this respect, Aβ has been regarded as a highly promising therapeutic target for AD treatment. Polyoxometalates(POMs) are a novel class of metallodrugs being developed as modulators of Aβ aggregation, owing to their negative charge, polarity, and three-dimensional *** traditional discrete inorganic complexes, POMs contain tens to hundreds of metal atoms,showcasing remarkable tunability and diversity in nuclearities, sizes, and shapes. The easily adjustable and structurally variable nature of POMs allows for their favorable interactions with Aβ. This mini-review presents a balanced overview of recent progress in using POMs to mitigate amyloidosis. Clear correlations between anti-amyloid activities and structural features of POMs are also elaborated in detail. Finally, we discuss the current challenges and future prospects of POMs in combating AD.

关键词： amyloid inhibition metallodrug polyoxometalate amyloid-β Alzheimer's disease

Identification and Characterization of the Nepl-like Protein UvNLP in Rice False Smut Fungus Ustilaginoidea virens

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Identification and Characterization of the Nepl-like Protein...

Development of rice conidiation media for Ustilaginoidea virens

中国植物病理学会2023年学术年会

作者： WANG Yufu qu jinsong XIE Songlin WANG Zuoqian YIN Liangfen YIN Weixiao LUO Chaoxi Key Laboratory of Crop Disease Monitoring & Safety Control in Hubei Province College of Plant Science and Technology Huazhong Agricultural University

The necrosis-and ethylene-inducing peptide 1(Nepl)-like proteins(NLPs) are a broadly distributed superfamily of protiens in plant-associated microorganisms,including bacteria,fungi,and *** are considered to be important virulence factors,and some have been well ***,the role of NLPs in Ustilaginoidea virens,the agent of rice false smut fungus,remains to be *** this study,a protein containing NLP-specific NPP1 domain was identified in *** and named ***;analysis revealed that UvNLP was a type 2 NLP,and realtime PCR revealed that UvNLP was highly expressed at 3 days post-inoculation.A yeast secretion assay demonstrated that UvNLP contained a functional signal peptide(SP).Furthermore,transient expression of UvNLP in Nicotiana benthamiana induced weak cell death,and replacement of the SP failed to enhance cell ***-time PCR indicated that UvNLP induced the expression of plant defence *** conclusion,we identified a secreted protein UvNLP in *** that was infection related and could inducted plant cell death and defence *** study providesd evidence that NLPs act as proteinaceous MAMPs,giving insight into the elucidation of the pathogenicity mechanism of *** UvNLP might be used as a potential plant defence inducer for disease control in practice.

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Development of rice conidiation media for Ustilaginoidea vir...

四川省草莓灰霉病菌对咯菌腈的抗性测定及其机制

中国植物病理学会2019年学术年会

作者： Wang Yufu Xie Songlin Liu Yi qu jinsong Lin Yang Huang Junbin Yin Weixiao Luo Chaoxi Department of Plant Pathology College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring and Safety Control in Hubei ProvinceHuazhong Agricultural University

Rice false smut,caused by the ascomycete Ustilaginoidea virens,is a serious disease of rice *** are very important infectious propagules of ***,but the ability of pathogenic isolates to produce conidia frequently decreases in culture,which impacts pathogenicity ***,we developed tissue media amended with rice leaves or panicles that stimulate conidiation by ***,rice leaf media more effectively increased conidiation than rice panicle media,and certain non-filtered tissue media were better than their filtered *** the tested media,the Indica rice leaf medium with 0.06 g/mL of Wanxian 98 leaves was most efficient at inducing conidiation by ***,and it was able to induce conidiation in conidiation-defective *** the conidia induced in rice tissue media were smaller,they were able to germinate on potato sucrose agar medium and infect rice *** method provides a foundation for the production of conidia by *** that will be widely applicable in its pathogenicity testing as well as in genetic analyses for false smut resistance in rice cultivars.

关键词： Rice false smut Ustilaginoidea virens Conidiation media Rice tissues Pathogenicity

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中国农业科学 2018年第22期51卷 4277-4287页

作者：贡常委秦旖曼屈劲松王学贵四川农业大学农学院/无公害农药研究实验室

【目的】灰霉病是草莓生产过程中的一种重要病害,严重影响了其产量和品质。论文旨在明确四川省草莓灰霉病菌对咯菌腈的抗性频率以及抗性机制,为草莓灰霉病的药剂防治提供理论依据。【方法】2016—2017年从四川成都、德阳、眉山、乐山及... 详细信息

【目的】灰霉病是草莓生产过程中的一种重要病害,严重影响了其产量和品质。论文旨在明确四川省草莓灰霉病菌对咯菌腈的抗性频率以及抗性机制,为草莓灰霉病的药剂防治提供理论依据。【方法】2016—2017年从四川成都、德阳、眉山、乐山及雅安等地采集草莓灰霉病样本,并分离纯化得到188株草莓灰霉病菌菌株。采用区分计量法测定188株灰霉病菌对咯菌腈的敏感性水平,采用菌丝生长速率抑制法测定咯菌腈对代表性菌株的毒力和渗透压敏感性,采用甘油铜比色法测定经咯菌腈处理的抗性菌株和敏感菌株甘油含量,采用分段测序对抗性菌株和敏感菌株Ⅲ型组氨酸激酶基因BOS1(BC1G0374)扩增测序,采用Swissmodle和I-TASSER预测和评价突变对BOS1的结构影响。【结果】188株灰霉病菌菌株中有8株表现为高抗,9株为中抗,43株为低抗,其余表现为敏感;咯菌腈对代表性菌株的EC50介于0.03—0.62μg·mL,代表性菌株的抗性倍数范围为2.2—45.9。NaCl浓度在1.25—10 g·L可刺激敏感菌株生长,浓度在1.25—20 g·L范围可刺激抗性菌株生长,但>40 g·L时则抑制菌丝生长,尤其对抗性菌株,抗性越高抑制作用越强;在正常条件下各代表性菌株甘油含量介于0.0025—0.0148μg·mL,且菌株的甘油含量与咯菌腈抗性没有明显的关联性,但在使用咯菌腈处理(0.1μg·mL)抗性菌株和敏感菌株后,甘油含量均上升,且抗性菌株甘油含量上升幅度明显低于敏感菌株。低抗菌株YAHY-13、CDCZ-2以及中抗菌株CDCZ-42在TAR和HAMP区域均发生突变,中抗菌株CDCZ-20和高抗菌株MYFC-10、CDCZ-43在TAR和REC区域均有突变,但CDCZ-20菌株在TAR区域位点是I365N,而两株高抗菌株在TAR区域位点是I365S。不同突变位置对BOS1区域结构有不同程度的影响,其中F127S、I365N、I365S、V1136I、A1259T均处于BOS1区域结构的无规则卷曲,但TAR区域I365N和I365S使区域结构无规则卷曲发生整体偏移。【结论】四川省已有部分地区草莓灰霉病菌对咯菌腈产生了抗性;相比敏感菌株,田间抗性菌株对渗透压的耐受能力增加,但当浓度超过耐受范围后对渗透胁迫高度敏感,药剂胁迫下田间抗性菌株甘油含量增加量显著小于敏感菌株;组氨酸激酶BOS1突变的位置和方式与灰霉病菌菌株对咯菌腈的抗性水平存在必然联系。

关键词：灰霉病菌咯菌腈抗药性渗透压甘油含量 BOS1

天然气储气库EG再生尾气脱水及热回收装置研发及应用

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江汉石油职工大学学报 2023年第3期36卷 30-33页

作者：屈劲松韩涛中石化新星湖北新能源开发有限公司湖北武汉430074 重庆清研理工智能控制技术研究院有限公司重庆400000

天然气储气库冬季供气过程常采用低温分离法脱水(注乙二醇EG+J-T阀制冷脱水)工艺。该工艺需要消耗热能用于乙二醇溶液再生,同时产生大量的高温含烃尾气,还需额外提供燃料气进行灼烧。本文依据能量平衡和相变理论,结合微流阻长流程高效... 详细信息

天然气储气库冬季供气过程常采用低温分离法脱水(注乙二醇EG+J-T阀制冷脱水)工艺。该工艺需要消耗热能用于乙二醇溶液再生,同时产生大量的高温含烃尾气,还需额外提供燃料气进行灼烧。本文依据能量平衡和相变理论,结合微流阻长流程高效换热技术、自流式分层冷却分离技术、变负荷热回收-稳定负荷供热缓存及控制技术成功开发了脱水及热回收撬装设备,并在某储气库投入应用。实际运行结果表明,采用新装置后,EG再生尾气处理过程中尾气脱水率达90%,灼烧炉燃烧负荷率降低67%,具有较大推广价值。

关键词：注乙二醇+J-T阀脱水燃烧负荷热回收

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稻曲病菌SIX效应蛋白参与调控植物免疫

植物保护学报 2022年第4期49卷 1004-1012页

作者：刘月冉孙庚屈劲松王毓富阴伟晓罗朝喜华中农业大学植物科技学院湖北省作物病害监测及安全控制重点实验室武汉430070

为明确稻曲病菌Ustilaginoidea virens的SIX(secreted in xylem)效应蛋白功能,利用生物信息学软件预测6个SIX1蛋白和1个SIX2蛋白的分泌特性,并通过酵母分泌系统对其分泌特性进行验证,在烟草上通过根癌农杆菌Agrobacterium tumefaciens... 详细信息

为明确稻曲病菌Ustilaginoidea virens的SIX(secreted in xylem)效应蛋白功能,利用生物信息学软件预测6个SIX1蛋白和1个SIX2蛋白的分泌特性,并通过酵母分泌系统对其分泌特性进行验证,在烟草上通过根癌农杆菌Agrobacterium tumefaciens介导的瞬时表达系统检测SIX蛋白对小鼠促凋亡蛋白BAX、致病疫霉Phytophthora infestans激发子INF1、大豆疫霉Phytophthora sojae病原相关分子模式糖基水解酶XEG1引起烟草细胞坏死的作用,在烟草上表达SIX蛋白后接种辣椒疫霉Phytophthora capsici并观察其对辣椒疫霉侵染的作用。结果显示:所检测的SIX蛋白中有5个含有预测的信号肽,2个被预测为非经典分泌蛋白,但经酵母分泌系统验证均具有分泌功能;大部分SIX蛋白能够抑制BAX、INF1、XEG1引起的烟草细胞坏死,但抑制坏死的情况并不完全一致;大部分含信号肽的SIX蛋白能够促进辣椒疫霉侵染,UV8b_3638去信号肽突变体也能够促进辣椒疫霉侵染。表明大多数SIX蛋白参与调控植物免疫,且能促进病原物的侵染。

关键词：稻曲病菌 SIX效应蛋白植物免疫致病性

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基于局部熵的点目标检测算法分析

火炮发射与控制学报 2014年第3期35卷 41-44页

作者：毛羽忻杨俊强曲劲松吴珍荣北京工业大学北京100124 中国兵器装备研究院北京100089

基于局部熵的目标检测与跟踪算法研究已有一些文章介绍,主要针对其应用、改进等方面。通过对点目标局部熵特点的深入研究,定量分析其在不同点目标对比度情况下的局部熵值变化,以及不同尺度子域对点目标作用,研究其中的相互关系,得出点... 详细信息

基于局部熵的目标检测与跟踪算法研究已有一些文章介绍,主要针对其应用、改进等方面。通过对点目标局部熵特点的深入研究,定量分析其在不同点目标对比度情况下的局部熵值变化,以及不同尺度子域对点目标作用,研究其中的相互关系,得出点目标的局部熵值定量分析算法。经不同灰度点目标与背景相同灰度差的情况下和具有相同对比度情况下点目标局部熵值的计算,验证了算法及分析的正确性。为点目标检测(如红外目标等)提供定量分析的基础。

关键词：局部熵小目标检测背景抑制

稻曲病菌bZIP转录因子UvbZIP12的功能研究

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植物病理学报 2022年第4期52卷 555-564页

作者：屈劲松王毓富王睿刘月冉谷丽凡周鹏阴伟晓罗朝喜华中农业大学植物科学技术学院作物病害监测和安全控制湖北省重点实验室武汉430070

稻曲病是由稻绿核菌(Ustilaginoidea virens)侵染水稻穗部引起的真菌病害,严重影响稻米的产量和品质。bZIP转录因子参与多种植物病原菌的生长发育和致病过程。稻曲病菌中有28个bZIP转录因子,但其具体功能尚未被研究。本研究对bZIP转录因... 详细信息

稻曲病是由稻绿核菌(Ustilaginoidea virens)侵染水稻穗部引起的真菌病害,严重影响稻米的产量和品质。bZIP转录因子参与多种植物病原菌的生长发育和致病过程。稻曲病菌中有28个bZIP转录因子,但其具体功能尚未被研究。本研究对bZIP转录因子UvbZIP12在稻曲病菌生长发育和致病过程中的功能进行分析。稻曲病菌接种水稻后,UvbZIP12基因表达量逐渐升高,在接种后3 d和5 d时具有最高表达量。对UvbZIP12基因的敲除转化子分析发现,敲除转化子ΔUvbZIP12在PSA、PDA、YTD培养基上生长速率显著加快,菌丝干重显著增加;产孢量减少;对NaCl和sorbitol的耐受性增强,对SDS、CR、CFW和H_(2)O_(2)的耐受性减弱;对水稻的致病力无明显变化。结果表明,稻曲病菌UvbZIP12参与调控生长发育和对非生物胁迫的耐受性,但不参与致病。

关键词：稻曲病菌 bZIP转录因子抗逆性致病性