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Coimmunization with IL-15 plasmid enhances the longevity of CD8 T cells induced by DNA encoding hepatitis B virus core antigen

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World Journal of Gastroenterology 2006年第29期12卷 4727-4735页

作者： Wei Zhang Sheng-Fu Dong Shu-Hui Sun Yuan Wang Guang-di Li di qu Institute of Molecular Medicine and Genetics Medical College of Georgia Augusta Georgia 30912 United States Key laboratory of Medical Molecular Virology Shanghai Medical College of Fudan University Shanghai 200032 China State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

AIM： To test the feasibility of delivering a plasmid encoding IL-15 as a DNA vaccine adjuvant for improving the immune responses induced by hepatitis B virus core gene DNA vaccine. METHODS： We used RT-PCR based strategies to develop IL-15 expression constructs. We first confirmed that the gene could be expressed in Escherichia coli due to the poor expression of IL-15. Then the bioactivity of IL-15 plasmid expression product was identified by CTLL-2 proliferation assay. One hundred micrograms of DNA from each of the IL-15 eukaryotic expressed plasmid and the recombinant plasmid harboring DNA encoding the 144 amino acids of the N-terminus of HBV core gene （abbreviated pHBc144） was used to co-immunize C57 BL/6 mice. The titer of anti-HBcIgG was detected by ELISA and the antigen-specific CD8^＋T cells （CD8^＋IFN-γ^＋ T cells） were detected by intracellular cytokine staining at different time points. RESULTS： After co-immunization by pIL-15 and pHBc144 DNA vaccine the antigen-specific CD8^＋ cells of mice increased gradually, the first peak of immune response appeared 14 d later, then the number of antigen-specific CD8^＋Ts cells decreased gradually and maintained at a steady level in 3 mo. After boosting, the number of antigen-specific CD8^＋T cells reached the second peak 10 d later with a double of the 1st peak, then the number of antigen-specific CD8^＋T cells decreased slowly. IL-15 as a gene adjuvant had no significant effect on humoral immune responses induced by hepatitis B virus core gene DNA vaccine, but increased the memory antigen-specific CD8^＋T cells induced by hepatitis B virus core gene DNA vaccine. CONCLUSION： DNA vaccine constructed by HBc Ag 1-144 amino acid induces effective cell immunity, and cytokine plasmid-delivered IL-15 enhances the longevity of CD8^＋ T cells.

关键词： Vaccine DNA vaccine Hepatitis B virus coreantigen

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Continuous-wave operation of InAs/InP quantum dot tunable external-cavity laser grown by metal-organic chemical vapor deposition

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Chinese Physics B 2021年第1期30卷 568-571页

作者： Yan Wang Shuai Luo Haiming Ji di qu Yidong Huang Innovation Center of Advanced Optoelectronic Chip Institute for Electronics and Information Technology in TianjinTsinghua UniversityTianjin 300467China Tianjin H-Chip Technology Group Corporation Tianjin 300467China Department of Electronic Engineering Tsinghua UniversityBeijing 100084China Key Laboratory of Semiconductor Materials Science Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

We demonstrate high-performance broadband tunable external-cavity lasers(ECLs) with the metal-organic chemical vapor deposition(MOCVD) grown In As/In P quantum dots(QDs) structures. Without cavity facet coatings, the 3-d B spectral bandwidth of the Fabry–Perot(FP) laser is approximately 10.8 nm, while the tuning bandwidth of ECLs is 45 *** with the anti-reflection(AR)/high-reflection(HR) facet coating, a 92 nm bandwidth tuning range has been obtained with the wavelength covering from 1414 nm to 1506 nm. In most of the tuning range, the threshold current density is lower than 1.5 k A/cm2. The maximum output power of 6.5 m W was achieved under a 500 m A injection *** achievements mentioned above were obtained under continuous-wave(CW) mode at room temperature(RT).

关键词： InAs/InP quantum dot external-cavity laser continuous-wave operation metal-organic chemical vapor deposition

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IFN-γ increases efficiency of DNA vaccine in protecting ducks against infection

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World Journal of Gastroenterology 2005年第32期11卷 4967-4973页

作者： Jian-Er Long Li-Na Huang Zhi-Qiang Qin Wen-Yi Wang di qu Department of Molecular Virology Shanghai Medical College Fudan University Shanghai 200032 China Shanghai Institute of Medical Genetics Shanghai Children's Hospital Shanghai Jiaotong University Shanghai 200040 China

AIM： To detect the effects of DNA vaccines in combination with duck IFN-γ gene on the protection of ducks against duck hepatitis B virus （DHBV） infection. METHODS： DuIFN-γ cDNA was cloned and expressed in COS-γ cells, and the antiviral activity of DuIFN-γ was detected and neutralized by specific antibodies, Ducks were vaccinated with DHBpreS/S DNA alone or coimmunized with plasmid expressing DuIFN-γ. DuIFN-γ mRNA in peripheral blood mononuclear cells （PBMCs） from immunized ducks was detected by semi-quantitative competitive RT-PCR. Anti-DHBpreS was titrated by enzyme-linked immunosorbent assay （EUSA）. DHBV DNA in sera and liver was detected by Southern blot hybridization, after ducks were challenged with high doses of DHBV. RESULTS： DuIFN-γ expressed by COS-γ was able to protect duck fibroblasts against vesicular stomatitis virus （VSV） infection in a dose-dependent fashion, and anti DuIFN-γ antibodies neutralized the antiviral effects. DuIFN-γ in the supernatant also inhibited the release of DHBV DNA from LMH-D2 cells. When ducks were co-immunized with DNA vaccine expressing DHBpreS/S and DuIFN-γ gene as an adjuvant, the level of DuIFN-γ mRNA in PBMCs was higher than that in ducks vaccinated with DHBpreS/S DNA alone. However, the titer of anti-DHBpreS elicited by DHBpreS/S DNA alone was higher than that co-immunized with DuIFN-γ gene and DHBpreS/S DNA. After being challenged with DHBV at high doses, the load of DHBV in sera dropped faster, and the amount of total DNA and cccDNA in the liver decreased more significantly in the group of ducks co-immunized with DuIFN-γ gene and DHBpreS/S DNA than in other groups.

关键词： Duck IFN-γ DHBV DNA vaccine Immuneadjuvant

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Intraoperative systemic vascular resistance is associated with postoperative nausea and vomiting after laparoscopic hysterectomy

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World Journal of Clinical Cases 2020年第20期8卷 4816-4825页

作者： Meng-di qu Meng-Yuan Zhang Gong-Ming Wang Zhun Wang Xu Wang Department of Anesthesiology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina

BACKGROUND The incidence of postoperative nausea and vomiting(PONV)in patients undergoing laparoscopic hysterectomy is very high compared with other surgeries,even when many prophylactic measures have been ***,the pathogenesis of PONV is *** sex,a history of motion sickness or PONV,nonsmokers,and perioperative opioid use are the most closely related *** the multiple risk factors,suboptimal gastrointestinal(GI)perfusion may be attributed to some cases of PONV,and increased systemic vascular resistance(SVR)may lead to GI *** hypothesis of this research was that SVR is related to *** To investigate the relationship between SVR and PONV in patients undergoing laparoscopic *** A total of 228 patients who underwent elective laparoscopic hysterectomy were included in this prospective observational *** was monitored using a noninvasive hemodynamic monitoring *** indices of SVR,the baseline,mean,area under the curve(AUC),and weighted AUC,were used for *** incidence and severity of nausea and vomiting were evaluated while patients were awake and throughout the intervals from 0 to 2 h,2 to 6 h,and 6 to 24 h starting upon arrival at the post-anesthesia care *** associations between various SVR indices and PONV were investigated by logistic regression.Pdiet and demonstrated poorer sleep quality on the first night

关键词： Postoperative nausea and vomiting Systemic vascular resistance Gastrointestinal perfusion Laparoscopic hysterectomy Prospective observational study Pneumoperitoneum

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Polyoxometalate-Based Ionic Frameworks for Highly Selective CO_(2) Capture and Separation

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CCS Chemistry 2021年第11期3卷 2676-2687页

作者： Fengxue Duan Xiaoting Liu di qu Bao Li Lixin Wu State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012

During the utilization of structural and functional advantages of polyoxometalates(POMs)for enhanced applications,a suitable assembly of these clusters in framework materials to act as binding nodes represents a promising *** contrast to well-developed coordination/covalent combinations,we have developed a convenient strategy to build porous structures of POMs with smaller-sized counterions as bridging ligands via ionic interactions to reinforce their capability in gas adsorption in parallel to metal-organic frameworks(MOFs)/covalent organic frameworks(COFs).With this goal,a series of POMs-based ionic frameworks(IFs)were constructed with triol-ligand modified Anderson-Evans-type clusters as building blocks,and their sodium counter-cations were used as *** three-dimensional(3D)open-frameworks obtained displayed unusually selective CO_(2) capturing capability and efficient separation from their N_(2),H_(2),and CH_(4) mixtures under low pressure at room *** the synthesized IFs,the cobalt-centered cluster exhibited the best performance for the uptake and selective separation of CO_(2) over N_(2) and CH_(4) in a range of 0-1 bar,while the nickel-centered cluster displayed the highest selectivity over H_(2) at 1 *** experiments based on real binary gas mixtures demonstrated that the cobalt-containing framework illustrated high performance in the actual gas separation and sustained stability against a simulated operating environment.

关键词： ionic frameworks polyoxometalates carbon dioxide adsorption gas separation

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Error concealment in pixel based Wyner-Ziv video coding

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High Technology Letters 2019年第3期25卷 340-346页

作者： Yuan Chengzong Han Chuanzhao Wang Yan Zhang Ning Zhang Zhen Zhu Xinzhong Li Xian qu di Key Laboratory of Ministry of Education and Ministry of Health Shanghai Medical College Fudan UniversityShanghai 200032P.R.China China Academy of Space Technology Beijing 100094P.R.China Shanghai Aerospace Elecetronic Technology Institute Shanghai 201109P.R.China

In the Wyner-Ziv(WZ) video coding paradigm, a virtual correlation channel is assumed between the quantized source and the side information(SI) at the decoder, and channel coding is applied to achieve compression. In this paper, errors caused by the virtual correlation channel are addressed and an error concealment approach is proposed for pixel-based WZ video coding. In the approach, errors after decoding are classified into two types. Type 1 errors are caused by residual bit errors after channel decoding, while type 2 errors are due to low quality of SI in part of a frame which causes SI not lying within the quantization bin of a decoded quantized pixel value. Two separate strategies are respectively designed to detect and conceal the two types of errors. Simulations are carried out and results are presented to demonstrate the effectiveness of the proposed approach.

关键词： Wyner-Ziv(WZ) video coding error detection error concealment side information(SI)

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Griffithsin with A Broad-Spectrum Antiviral Activity by Binding Glycans in Viral Glycoprotein Exhibits Strong Synergistic Effect in Combination with A Pan-Coronavirus Fusion Inhibitor Targeting SARS-CoV-2 Spike S2 Subunit

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Virologica Sinica 2020年第6期35卷 857-860页

作者： Yanxing Cai Wei Xu Chenjian Gu Xia Cai di qu Lu Lu Youhua Xie Shibo Jiang Key Laboratory of Medical Molecular Virology(MOE/NHC/CAMS) School of Basic Medical Sciences and BSL-3 FacilityFudan UniversityShanghai 200032China

Dear Editor,The pandemic of coronavirus disease 2019(COVID-19)caused by SARS-Co V-2 has posed a significant threat to global public health and economy,thus calling for the rapid development of effective therapeutics and *** existing medicines with clinical safety profiles offers a more rapid hope of combating COVID-19pandemic than developing a new therapeutic.

关键词： clinical Strong Griffith

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How EFL Learners from China and ASEAN Countries Politely Express disagreement in an English Context:A Comparative Study

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教育研究前沿（中英文版） 2020年第4期10卷 275-281页

作者： Shu Zhang Fang He di qu General Teaching Department Nanning University530001China Department of Science High School Northeast Yucai School110000China

This paper aims to make a comparative study of cross-cultural communication upon a special speech act-"disagreement".38 undergraduates from China and 30 undergraduates from ASEAN countries were *** responded to the DCT(discourse completion test).Five contexts were selected and detailed descriptions of the scenarios were *** distance and gender were selected as the main variants in this *** the results,we found that both groups of undergraduates generally ten d to use the same politeness strategies according to the same social distance,but gender was a more significant factor in politeness strategies adoption among EFL learners from ASEAN *** tend to use negative strategies more than males *** can conclude from the results that EFL learners from China and ASEAN countries incline to adopt the same politeness strategies in English context,but females from ASEAN countries are less likely to say“no”directly to express their disagreement compared to their *** findings may offer reference to both sides during the pragmatic occasions of communicating.

关键词： disagreement Politeness Strategies Social distance Gender ASEAN EFL Learners

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Filamentation initiated by Cas2 and its association with the acquisition process in cells

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International Journal of Oral Science 2019年第4期11卷 300-306页

作者： Lei Wang Xin Yu Mengjie Li Guiqin Sun Lin Zou Tiansheng Li Linlin Hou Yameng Guo Danfeng Shen di qu Xunjia Cheng Li Chen Department of Medical Microbiology Key Laboratory of Medical Molecular Virology of Ministries of Education and Health School of Basic Medical Sciences Fudan University 131 Dongan Road Shanghai China and College of Medical Technology Zhejiang Chinese Medical University

Cas1-and-Cas2-mediated new spacer acquisition is an essential process for bacterial adaptive *** process is critical for the ecology of the oral microflora and oral *** molecular mechanisms for spacer acquisition are known,it has never been established if this process is associated with the morphological changes of *** this study,we demonstrated a novel Cas2-induced filamentation phenotype in *** that was regulated by co-expression of the Cas1 protein.A 30 amino acid motif at the carboxyl terminus of Cas2 is necessary for this *** imaging analysis,we provided evidence to argue that Cas-induced filamentation is a step coupled with new spacer acquisition during which filaments are characterised by polyploidy with asymmetric cell *** work may open new opportunities to investigate the adaptive immune response and microbial balance for oral health.

关键词： function. process initiated

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Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis

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Bioactive Materials 2024年第2期32卷 488-501页

作者： Wei Zhang Zhuo Wan di qu Wenqi Sun Liang Zhang Yuan Liang Lei Pan Hua Jiang Zichen Ye Mengying Wei Lijun Yuan Guodong Yang Faguang Jin Department of Pulmonary and Critical Care Medicine Tangdu HospitalFourth Military Medical UniversityXi’anShaanxi710038China Department of Hematology Tangdu HospitalFourth Military Medical UniversityXi’anShaanxi710038China Department of Cardiology Xijing HospitalFourth Military Medical UniversityXi’anShaanxi710032China Department of Clinical Pharmacy General Hospital of Western Theater CommandChengduSichuan610083China Department of Ultrasound diagnostics Tangdu HospitalFourth Military Medical UniversityXi’anShaanxi710038China Department of Health Service Health Service Training BaseFourth Military Medical UniversityXi’anShaanxi710032China State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Department of Biochemistry and Molecular BiologyFourth Military Medical UniversityXi’anShaanxi710032China

Pulmonary fibrosis(PF)is a devastating lung disease with limited treatment *** this pathological process,the profibrogenic macrophage subpopulation plays a crucial role,making the characterization of this subpopulation fundamentally *** present study revealed a positive correlation between pulmonary macrophages with higher mitochondrial mass(Mø^(mitohigh))and *** the Mø^(mitohigh)subpopulation of CD206^(+)M2,characterized by higher expression of dynamin 1-like(Drp1),as determined by flow cytometry and RNA-seq analysis,a therapeutic intervention was developed using an exosome-based formula composed of pathfinder and therapeutics.A pathfinder exosome called“exosome^(MMP19)(Exo^(MMP19))”,was constructed to display matrix metalloproteinase-19(^(MMP19))on the surface to locally break down the excessive extracellular matrix(ECM)in the fibrotic lung.A therapeutic exosome called“exosome therapeutics(Exo^(Tx))”,was engineered to display D-mannose on the surface while encapsulating siDrp1 *** delivery of Exo^(MMP19)degraded excessive ECM and thus paved the way for Exo^(Tx)to be delivered into Mø^(mitohigh),where Exo^(Tx)inhibited mitochondrial fission and alleviated *** study has not only identified Mø^(mitohigh)as profibrotic macrophages but it has also provided a potent strategy to reverse PF via a combination of formulated exosomes.

关键词： Pulmonary fibrosis Macrophages Mitochondrial fission Drp1 Exosomes

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