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Carbon dioxide partial pressure and its diffusion flux in karst surface aquatic ecosystems:a review

地球化学学报(英文) 2023年第5期42卷 943-960页

作者： Xingxing Cao qixin wu Wanfa Wang Pan wu College of Resources and Environmental Engineering Guizhou UniversityGuiyang 550025China Key Laboratory of Karst Georesources and Environment Ministry of EducationGuizhou UniversityGuiyang 550025China

Carbon dioxide(CO_(2))emissions from aquatic ecosystems are an important component of the karst carbon cycle process and also a key indicator for assessing the effect of karst carbon sinks.This paper reviewed the CO_(2)partial pressure(pCO_(2))and its diffusion flux(FCO_(2))in karst surface aquatic ecosystems,mainly rivers,lakes,and reservoirs,and their influencing factors summarized the methods for monitoring CO_(2)emissions in karst aquatic ecosystems and discussed their adaptation conditions in karst areas.The pCO_(2)and FCO_(2)decreased in the order of rivers>reservoirs>lakes,and the values in karst lakes were eventually significantly lower than those in global lakes.The pCO_(2)and FCO_(2)of karst aquatic ecosystems had patterns of variation with diurnal,seasonal,water depth and hydrological cycles,and spatial and temporal hetero-geneity.The sources of CO_(2)in karst waters are influenced by both internal and external sources,and the key spatial and temporal factors affecting the CO_(2)emissions from karst rivers,lakes,and reservoirs were determined in terms of physicochemical indicators,biological factors,and bio-genic elements;additionally,the process of human activity interference on CO_(2)emissions was discussed.Finally,a conceptual model illustrating the impacts of urban devel-opment,agriculture,mining,and dam construction on the CO_(2)emissions at the karst surface aquatic ecosystem is presented.Meanwhile,based on the disadvantages existing in current research,we proposed several important research fields related to CO_(2)emissions from karst surface aquatic ecosystems.

关键词： Karst area River Lake Reservoir Partial pressure of CO_(2) CO_(2)diffusion flux

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Computer-aided diagnosis of retinopathy based on vision transformer

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Journal of Innovative Optical Health Sciences 2022年第2期15卷 49-57页

作者： Zhencun Jiang Lingyang Wang qixin wu Yilei Shao Meixiao Shen Wenping Jiang Cuixia Dai Shanghai Institute of Technology 100 Haiquan Road Shanghai 201418China School of Ophthalmology and Optometry Wenzhou Medical University Xueyuan Road 270 WenzhouZhejiang 325027China

Age-related Macular Degeneration(AMD)and Diabetic Macular Edema(DME)are two com-mon retinal diseases for elder people that may ultimately cause irreversible blindness.Timely and accurate diagnosis is essential for the treatment of these diseases.In recent years,computer-aided diagnosis(CAD)has been deeply investigated and effectively used for rapid and early diagnosis.In this paper,we proposed a method of CAD using vision transformer to analyze optical co-herence tomography(OCT)images and to automatically discriminate AMD,DME,and normal eyes.A classification accuracy of 99.69%was achieved.After the model pruning,the recognition time reached 0.010 s and the classification accuracy did not drop.Compared with the Con-volutional Neural Network(CNN)image classification models(VGG16,Resnet50,Densenet121,and EfficientNet),vision transformer after pruning exhibited better recognition ability.Results show that vision transformer is an improved alternative to diagnose retinal diseases more accurately.

关键词： Vision transformer OCT image classi¯cation retinopathy computer-aided diagnosis model pruning

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Bailing capsule(百令胶囊) alleviates autoimmune thyroiditis via regulating peroxisome proliferator-activated receptor signaling pathway: a multi-omics analysis

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Journal of Traditional Chinese Medicine 2024年

作者： WANG qixin XU Liting wu Jiangpeng HE Xueling TANG Huan CHENG Guangqing LU Tianming DAI Chuanhao GUO Qiuyan WANG Jigang State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Artemisinin Research Center and Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences School of Chinese Materia Medica and State Key Laboratory of Component-based Chinese Medicine Tianjin University of Traditional Chinese Medicine

OBJECTIVE: To investigate the efficacy and potential mechanism of Bailing capsule (百令胶囊， BL) anti-autoimmune thyroiditis (AIT). METHODS: Based on the AIT rat model， the effect of BL in alleviating AIT was evaluated by detecting serum thyroid index free triiodothyronine (FT3)， free thyroxine (FT4)， thyroid-stimulating hormone (TSH)， thyroglobulin antibody (TGAb)， thyroid peroxidase antibody (TPOAb)， and inflammatory factors Interferon-gamma (IFN-γ)， Interleukin-4， -10， and -12 (IL-4， IL-10， and IL-12) as well as thyroid tissue Hematoxylin-eosin (HE) staining and ultrastructure observation. The mechanism of BL was explored by combining transcriptome and proteome analysis， and further verified by Western blot (WB). RESULTS: BL effectively reduced serum FT3， FT4， TGAb， and TPOAb levels in AIT rats， restored TSH balance， inhibited the release of pro-inflammatory cytokines IFN-γ and IL-12， promoted the production of anti-inflammatory cytokines IL-4 and IL-10， and significantly reduced IFN-γ/IL-4 and IL-12/IL-10， improved thyroid follicular structure， and protected thyroid tissue from injury. Kyoto Encyclopedia of Genes and Genomes and protein interaction network analysis showed that BL affected the expression of fatty acid-binding protein 4， acyl-CoA synthetase long-chain family member 1， and acyl-CoA dehydrogenase long chain to regulate the peroxisome proliferator-activated receptor signaling pathway， thereby inhibiting the fatty acid metabolism and the inflammatory state of AIT rats. CONCLUSIONS: BL could effectively reduce thyroid inflammation in AIT model rats. The possible BL mechanism was to regulate the peroxisome proliferator-activated receptor signaling pathway and inhibit fatty acid metabolism. This study suggested that BL has the potential to be used in clinical treatment of AIT.

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Chemical characteristics of rainwater in karst rural areas,Guizhou Province, Southwest China

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Acta Geochimica 2017年第3期36卷 572-576页

作者： Pin Lü Guilin Han qixin wu School of Water Resources and Environment China University of Geosciences (Beijing) Key Laboratory of Karst Environment and Geohazard Ministry of Land and Resources Guizhou University

The chemical composition of rainwater has been studied in a karst rural area from September 2012 to August 2013 in Guizhou Province,Southwest China.The results indicated that the VWM value of p H was 5.4,varied from 4.6 to 6.9.Ca^(2+)and NH_4^+were the major cations,and SO_4^(2-)was the dominant anion.Neutralization factors show that the acid was mainly neutralized by Ca^(2+),NH_4^+and Mg^(2+).Investigations of correlation coefficients and enrichment factors revealed that Ca^(2+)and Mg^(2+)were mainly crust origins,and NH_4^+was from agriculture and livestock manure.SO_4^(2-)and NO_3^-were mainly from anthropogenic sources.

关键词：中国西南部农村地区喀斯特贵州省雨水化工化学成分富集因素

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Chemical weathering and CO_2 consumption of a high-erosion-rate karstic river: a case study of the Sanchahe River, southwest China

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Chinese Journal Of Geochemistry 2015年第4期34卷 601-609页

作者： Yanling An Yiliang Hou qixin wu Lin Qing Longbo Li Key Laboratory of Karst Environment and Geohazard Prevention Ministry of Education Guizhou University State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Guiyang Engineering Corporation China Power

The Sanchahe River in southwest China is a tributary of the wujiang River and experiences high erosion rates. Geochemical analysis was conducted on Sanchahe River basin samples collected in the wet and dry seasons of 2014 in order to better understand local chemical weathering processes, anthropogenic influences, and associated CO2 consumption. The samples' total dissolved solid concentrations were found to be significantly higher than that of the global river average. Ca2+was the dominant cation in the samples and accounted for 64 % and73 % of the total cations in the dry and wet seasons,respectively. HCO3-and SO42-were the dominant anions,accounting for 92 % of the total anions. Stoichiometry analyses of the river waters suggested that the water chemistry is controlled by carbonate dissolution by both carbonic and sulfuric acid. The chemical weathering rates of carbonate and silicate evaporites in the Sanchahe River basin were estimated to be approximately 109.2 and 11.0 t/(km2a), respectively, much higher than both the global mean values and the wujiang River, a typical karstic river.The CO2 consumption by carbonate and silicate weathering are estimated to be 597.4 9 103 and 325.5 9 103mol/(km2a), which are much higher than corresponding values in the wujiang River, indicating a high erosion rate in the Sanchahe River basin.

关键词：化学风化中国西南 CO2 消耗量岩溶侵蚀率阳离子总量地球化学分析

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Sensitive system calls based packed malware variants detection using principal component initialized MultiLayers neural networks

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Cybersecurity 2018年第1期1卷 185-197页

作者： Jixin Zhang Kehuan Zhang Zheng Qin Hui Yin qixin wu Department of Information Engineering Chinese University of Hong KongHong KongChina College of Computer Science and Electronic Engineering Hunan UniversityHunanChina

Malware detection has become mission sensitive as its threats spread from computer systems to Internet of things systems.Modern malware variants are generally equipped with sophisticated packers,which allow them bypass modern machine learning based detection systems.To detect packed malware variants,unpacking techniques and dynamic malware analysis are the two choices.However,unpacking techniques cannot always be useful since there exist some packers such as private packers which are hard to unpack.Although dynamic malware analysis can obtain the running behaviours of executables,the unpacking behaviours of packers add noisy information to the real behaviours of executables,which has a bad affect on accuracy.To overcome these challenges,in this paper,we propose a new method which first extracts a series of system calls which is sensitive to malicious behaviours,then use principal component analysis to extract features of these sensitive system calls,and finally adopt multi-layers neural networks to classify the features of malware variants and legitimate ones.Theoretical analysis and real-life experimental results show that our packed malware variants detection technique is comparable with the the state-of-art methods in terms of accuracy.Our approach can achieve more than 95.6\%of detection accuracy and 0.048 s of classification time cost.

关键词： Malware variants Multi-layers neural networks Principal component analysis Sensitive system calls Sophisticated packers

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考虑倒伏与粮仓容积的再生稻头季机收路径规划

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农业工程学报 2024年第12期40卷 33-45页

作者：张国忠凌高旻季超吴擎康启新唐楠锐赵状状华中农业大学工学院武汉430070 农业农村部长江中下游农业装备重点实验室武汉430070 中国热带农业科学院农业机械研究所湛江524013

为提升机具作业效率和质量、减少土壤碾压等,该研究提出一种同时考虑作物倒伏状态、碾压面积和收获机粮仓容积的路径规划算法(harvester grain bin capacitated arc routing problem,HGBCARP)。该算法由作业信息处理模块和作业路径规划... 详细信息

为提升机具作业效率和质量、减少土壤碾压等,该研究提出一种同时考虑作物倒伏状态、碾压面积和收获机粮仓容积的路径规划算法(harvester grain bin capacitated arc routing problem,HGBCARP)。该算法由作业信息处理模块和作业路径规划模块组成,作业信息处理模块将农田边界、卸粮点位置、作物倒伏方向及面积、位置等信息转化成可处理的数据形式并传输给路径规划模块,然后由路径规划模块进行作业行方向划分、作业行遍历顺序寻优、转弯方式生成、碾压面积计算等,最终得到最优路径规划结果。采用改进遗传算法,分别以3种再生稻收获机、2种传统水稻收获机和3种不同田块为对象,以行驶路径长度、碾压面积、收获粮食量为评价指标,采用回转式收获路径和HGBCARP式收获路径规划开展对比试验。结果表明,HGBCARP式比回转式收获路径碾压面积每公顷减少11.79%~27.20%,可使倒伏的机收头季稻增收1.64%~1.95%;同时在3种不同田块条件下进行仿真试验,HGBCARP式比回转式收获路径可使碾压面积每公顷减少7.25%~20.09%。使用电动无人履带式底盘对不同收获路径进行田间模拟收获试验,HGBCARP式收获路径与传统牛耕往复式及回转式收获路径相比,碾压面积减少约11.21%~28.03%,在路径长度减少约6.81%~23.46%,验证了HGBCARP式路径规划方法的有效性,研究结果可为智能化作业路径规划研究提供参考。

关键词：作物倒伏低碾压轨迹规划弧路径规划问题

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化工企业初期雨水收集处理的环境经济效应

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环境污染与防治 2024年第6期46卷 922-926页

作者：戴亦凡杨梦杰徐露陈佩贤吴兆康徐启新华东师范大学生态与环境科学学院上海城市化生态过程和生态恢复重点实验室上海200241 上海污染控制与生态安全研究院上海200092

以上海化学工业区某典型化工企业57场降雨径流在线监测数据为基础,模拟不同初期雨水收集深度情景下化工企业雨水排放口水质达标率与雨水系统运行费用的变化。分析结果显示,化工企业收集20 mm初期雨水后,预计污染降雨径流流量下降32%,雨... 详细信息

以上海化学工业区某典型化工企业57场降雨径流在线监测数据为基础,模拟不同初期雨水收集深度情景下化工企业雨水排放口水质达标率与雨水系统运行费用的变化。分析结果显示,化工企业收集20 mm初期雨水后,预计污染降雨径流流量下降32%,雨水水质超标频率降低72%,企业雨水系统运行费用减少32%。因此,化工企业做好初期雨水管控,能够显著减少雨水系统运行费用,同时可以有效降低企业水环境污染风险。

关键词：化工企业初期雨水初期雨水收集深度环境效应经济效应

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柯乐猪KRT10基因多态性及其与繁殖性状的关联分析

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中国畜牧兽医 2024年第6期51卷 2508-2516页

作者：赵永杨齐心李维熊力杨红文王春源吴燕向进张依裕贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室贵州省动物遗传育种与繁殖重点实验室贵州大学香猪研究所贵阳550025 贵州省畜禽遗传资源管理站贵阳550001 贵州省动物疫病预防控制中心贵阳550001

【目的】研究角蛋白10(keratin 10,KRT10)基因多态性对柯乐猪繁殖性状的影响,以提高柯乐猪的繁殖力。【方法】以255头柯乐猪为研究对象,采用PCR产物测序、DANMAN序列比对软件及人工校对相结合的方法鉴定KRT10基因单核苷酸多态性(single ... 详细信息

【目的】研究角蛋白10(keratin 10,KRT10)基因多态性对柯乐猪繁殖性状的影响,以提高柯乐猪的繁殖力。【方法】以255头柯乐猪为研究对象,采用PCR产物测序、DANMAN序列比对软件及人工校对相结合的方法鉴定KRT10基因单核苷酸多态性(single nucleotide polymorphism, SNP)位点,利用SHEsis在线软件分析SNP位点的群体遗传特性,通过RNAfold在线工具对SNP位点进行生物信息学分析,使用SPSS 22.0软件中的一般线性模型分析KRT10基因SNP位点与柯乐猪繁殖性状的关联性。【结果】在柯乐猪KRT10基因中共鉴定到3个SNPs位点:第4内含子的g.21643703 C>T和g.21643714 G>A;第5外显子的g.21643741 G>A。g.21643741 G>A突变导致密码子由AAG突变为AAA,编码氨基酸均为赖氨酸(K),为同义突变,引起编码的mRNA二级结构发生改变。3个SNPs位点均检测到3种基因型,g.21643703 C>T和g.21643741 G>A属于中度多态位点(0.25A)属于低度多态位点(PICT和g.21643741 G>A位点均处于Hardy-Weinberg平衡状态(P>0.05),g.21643714 G>A位点显著偏离Hardy-Weinberg平衡状态(PT位点对柯乐猪初生窝重、断奶仔数和断奶窝重的影响均达到显著水平(PA位点对柯乐猪总产仔数和断奶窝重的影响均达到显著水平(PA位点对柯乐猪总产仔数、产活仔数、断奶仔数和断奶窝重的影响均达到显著水平(P<0.05)。双倍型H3H3对柯乐猪总产仔数、产活仔数、断奶仔数和断奶窝重的影响最明显。【结论】KRT10基因中存在的3个SNPs位点对柯乐猪繁殖性状有显著影响,其中H3H3为有利双倍型,可作为柯乐猪繁殖性状选择的遗传标记。

关键词：柯乐猪 KRT10基因单核苷酸多态性(SNP) 繁殖性状

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罗伊氏乳杆菌替代抗生素对仔猪肠道miRNA表达的影响

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中国畜牧兽医 2024年第1期51卷 74-85页

作者：李虹仪杨雪芬林泽森吴晓菲吕祺欣余佳莉张茂韶关学院生物与农业学院韶关512000 广东省农业科学院动物科学研究所猪禽种业全国重点实验室农业农村部华南动物营养与饲料重点实验室广东省畜禽育种与营养研究重点实验室岭南现代农业科学与技术广东省实验室茂名分中心广州510640

【目的】研究禁抗后饲粮中添加罗伊氏乳杆菌LR1对仔猪各肠段微小RNA(microRNA,miRNA)表达的影响,为罗伊氏乳杆菌LR1调控仔猪肠道功能的机制研究筛选潜在miRNA。【方法】选取21日龄体重相近的杜×长×大断奶仔猪144头,随机分为3组,每组8... 详细信息

【目的】研究禁抗后饲粮中添加罗伊氏乳杆菌LR1对仔猪各肠段微小RNA(microRNA,miRNA)表达的影响,为罗伊氏乳杆菌LR1调控仔猪肠道功能的机制研究筛选潜在miRNA。【方法】选取21日龄体重相近的杜×长×大断奶仔猪144头,随机分为3组,每组8个重复,每个重复6头仔猪。对照组仔猪饲喂基础饲粮,抗生素组和益生菌组仔猪在饲喂基础饲粮的基础上分别添加复合抗生素(100 mg/kg喹乙醇+75 mg/kg金霉素)及5×10^(10)CFU罗伊氏乳杆菌LR1,试验期42 d。试验结束后每个重复随机屠宰1头仔猪,采集空肠组织进行miRNA高通量测序分析,采用实时荧光定量PCR检测筛选miRNA在仔猪十二指肠、空肠、回肠和结肠中的表达量。【结果】饲粮中添加罗伊氏乳杆菌LR1可引起仔猪肠道组织miRNA表达发生明显变化,改变了空肠高表达miRNA的排序。差异表达miRNA的潜在靶基因主要富集在代谢相关通路及PI3K-Akt、MAPK、Chemokine、Ras信号通路。实时荧光定量PCR显示,3个组间仔猪空肠差异miRNA的表达结果与转录组测序结果大致相同,其中,表达上调的miRNA在对照组和抗生素组间表达趋势较为一致,表达下调的miRNA在对照组和益生菌组间表达趋势较为一致,而肠道高表达miRNA在3个组中均有较为一致的表达趋势。【结论】禁抗后饲粮中添加罗伊氏乳杆菌LR1会引起肠道miRNA表达发生变化,筛选出的差异表达miRNA可为罗伊氏乳杆菌LR1调控仔猪肠道发育及营养吸收代谢等研究提供基础。

关键词：罗伊氏乳杆菌仔猪肠道miRNA

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