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Natural killer cell membrane doped supramolecular nanoplatform with immuno-modulatory functions for immuno-enhanced tumor phototherapy

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Chinese Chemical Letters 2024年第10期35卷 231-238页

作者： Ying Gao Rong Zhou qiwen Wang Shaolong Qi yuanyuan Lv Shuang Liu Jie Shen Guocan yu Institute of Pharmaceutics College of Pharmaceutical Sciences Zhejiang University Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province School of Medicine Hangzhou City University Department of Cardiology The First Affiliated Hospital Zhejiang University School of Medicine MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology Department of Chemistry Tsinghua University Emergency Department State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Science and Peking Union Medical College

Photodynamic therapy(PDT) has garnered significant attention as a promising approach to cancer therapy, harnessing the combined benefits of localized light treatment and the accompanying host immune response. In this study, we engineered an immuno-enhanced PDT nanoplatform, denoted as HM@p-MOF(hybrid membrane@porphyrin-metal organic framework). The core porphyrin-MOF was cloaked with a hybrid membrane derived from B16F10 cancer cells and NK cells, resulting in enhanced stability. In both in vitro and in vivo experiments, our finding demonstrated that the hybrid membrane conferred dual targeting capabilities to the nanoplatform, leveraging the unique properties of the B16F10 membrane and NK membrane to augment immunogenic cell death(ICD) induced by photodynamic effects. Additionally,in conjunction with the immunomodulatory functions of the NK cell membrane, we observed an expansion of in situ immune infiltration leading to a systemic immune response. The HM@p-MOF nanoplatform exhibited the capacity to not only inhibit the growth of mouse melanoma but also suppress metastasis. This innovative HM@p-MOF nanoplatform present a viable strategy to enhance phototherapeutic efficacy for both localized and metastatic tumors. It provides a direction for the fabrication of biomimetic nanomedicines possessing immuno-modulatory function.

关键词： Biomimetic Cell membrane Metal-organic framework Phototherapy Tumor targeting

邻苯二甲酸二(2-乙基己基)酯致人脐静脉内皮细胞氧化损伤及白藜芦醇的修复作用

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江苏大学学报（医学版） 2024年第1期34卷 37-43页

作者：王子璇刘伟蔺博涵李涛杨琴虞绮雯朱伟孙晓春江苏大学医学院江苏镇江212013 江苏大学第四附属医院妇女保健科江苏镇江212000

目的:探究邻苯二甲酸二(2-乙基己基)酯(di-2-ethylhexyl phthalate,DEHP)诱导的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)的氧化损伤,以及白藜芦醇对该损伤的修复作用。方法:通过CCK8实验筛选DEHP的损伤浓度和... 详细信息

目的:探究邻苯二甲酸二(2-乙基己基)酯(di-2-ethylhexyl phthalate,DEHP)诱导的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)的氧化损伤,以及白藜芦醇对该损伤的修复作用。方法:通过CCK8实验筛选DEHP的损伤浓度和白藜芦醇的修复浓度;化学分光光度比色法检测HUVEC丙二醛和超氧化物歧化酶(SOD)的不同表达情况;活性氧荧光探针检测不同处理组HUVEC内活性氧水平;JC-1试剂盒检测不同组HUVEC线粒体膜电位;CCK8实验、Transwell实验、划痕愈合实验观察HUVEC增殖、迁移能力;细胞流式实验、蛋白质印迹实验观察HUVEC的凋亡情况和凋亡相关蛋白的表达。结果:80μmol/L DEHP作用HUVEC 24 h后,细胞增殖能力明显降低(P<0.01),40μmol/L白藜芦醇对细胞无明显毒性;损伤组细胞的丙二醛水平和SOD活性较对照组均明显上升(P均<0.01),修复组丙二醛水平和SOD活性较损伤组均明显下降(P均<0.05);损伤组细胞的活性氧表达较对照组明显上升(P<0.01),修复组较损伤组明显下降(P<0.01);损伤组细胞的线粒体膜电位较对照组明显下降(P<0.01),修复组较损伤组明显上升(P<0.01);损伤组细胞的增殖、迁移、成管能力较对照组明显下降(P均<0.05),修复组较损伤组明显上升(P均<0.05);损伤组细胞的凋亡水平较对照组明显上升(P<0.05),修复组较损伤组明显下降(P<0.05)。结论:DEHP能够通过氧化应激途径抑制HUVEC的增殖、迁移和成管能力,并诱导其凋亡,而白藜芦醇可以有效修复该种损伤。

关键词：人脐静脉内皮细胞邻苯二甲酸二(2-乙基己基)酯白藜芦醇氧化应激凋亡氧化损伤修复

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Pressure-responsive pH-triggered layer-by-layer coating of paclitaxel-eluting balloon catheters for instant high-dose drug delivery for percutaneous coronary artery intervention

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Nano Research 2024年第10期17卷 9141-9151页

作者： Jeong yu Lee Shonit Nair Sharma Sung-yu Hong Young-Guk Ko Yangsoo Jang Minjae Do Michael Christopher Stark qiwen Peng Benjamin Allen Mossburg Bo Chan Seo yuhan Lee Department of Materials Science and Engineering Korea Advanced Institute of Science and TechnologyDaejeon 305-701Republic of Korea Department of Anesthesiology Perioperativeand Pain MedicineCenter for Accelerated Medical Innovation&Center for NanomedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115USA Department of Biological Engineering Massachusetts Institute of TechnologyCambridgeMA 02139USA Cardiovascular Product Evaluation Center Yonsei University College of MedicineSeoul 120-752Republic of Korea Division of Cardiology Department of Internal MedicineSeverance Cardiovascular HospitalYonsei University College of MedicineSeoul 120-752Republic of Korea

Cardiovascular diseases remain the leading cause of mortality worldwide,with percutaneous coronary interventions(PCI)using drug-eluting stents(DES)as the standard treatment for coronary artery *** advancements in DES technology,challenges such as restenosis and stent thrombosis still necessitate further interventions and carry considerable risks,highlighting an urgent need for a novel therapeutic approach with lower restenosis rates and reduced reintervention *** balloons(DEBs)coated with anti-proliferative agents offer a novel method of local drug delivery for reducing restenosis by directly administering drugs to the lesion ***,efficient delivery of drugs within the brief procedural window while minimizing drug loss during maneuvering to the target site remains a ***,we developed a pressure-responsive and pH-triggered,paclitaxel(PTX)coated DEB catheter for rapid and high-dose delivery of paclitaxel to the lesion sites,overcoming the limitations of drug dispersion and inefficiencies associated with conventional *** the charge reversion of albumin around its isoelectric point,paclitaxel-loaded human serum albumin nanoparticles(PTX-HSA-NPs)and heparin were coated using a layer-by-layer deposition method at pH *** exposure to physiological pH(i.e.,7.4),the coating is rapidly released from the balloon *** with a protective shellac coating,the DEB achieved controlled release of high-dose paclitaxel to the arterial tissue in vivo(up to 400μg per gram tissue),exceeding current DEBs in the *** results suggest the pH-responsive,multi-layer coated DEB’s potential for superior procedural outcomes,addressing a critical unmet need in PCI.

关键词： drug-eluting balloon layer-by-layer coating coronary artery disease paclitaxel

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圆柱固-液结构声子晶体的全反射隧穿效应

压电与声光 2012年第4期34卷 581-584,592页

作者：刘启能于奇文重庆工商大学废油资源化技术与装备教育部工程研究中心重庆400067 重庆工商大学计算机科学与信息工程学院重庆400067 辽阳鸿宇晶体有限公司辽宁辽阳111000

为了研究一维固-液结构圆柱形声子晶体中弹性波的全反射隧穿效应,利用转移矩阵法计算了弹性波在大于全反射角入射一维圆柱形声子晶体的透射率。在透射波中发现了全反射隧穿效应,得出了全反射隧穿峰的频率随模式量子数的变化特征,全反射... 详细信息

为了研究一维固-液结构圆柱形声子晶体中弹性波的全反射隧穿效应,利用转移矩阵法计算了弹性波在大于全反射角入射一维圆柱形声子晶体的透射率。在透射波中发现了全反射隧穿效应,得出了全反射隧穿峰的频率随模式量子数的变化特征,全反射隧穿峰的频率随圆柱半径的的变化特征,全反射隧穿峰的峰数随周期数的变化特征。并利用波的量子理论和倏逝波的理论对一维声子晶体的隧穿效应作出了定性的理论解释。

关键词：圆柱声子晶体全反射隧穿效应模式

Overexpression of tonoplast Ca^(2+)-ATPase in guard cells synergistically enhances stomatal opening and drought tolerance

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Journal of Integrative Plant Biology 2024年第8期66卷 1587-1602页

作者： Jinghan Su Bingqing He Peiyuan Li Baiyang yu qiwen Cen Lingfeng Xia Yi Jing Feibo Wu Rucha Karnik Dawei Xue Michael R.Blatt Yizhou Wang Institute of Crop Science Zhejiang UniversityHangzhou 310058China Laboratory of Plant Physiology and Biophysics University of GlasgowGlasgow G128QQUK BGI Research Sanya 572025China College of Life and Environmental Sciences Hangzhou Normal UniversityHangzhou 311121China Zhejiang Provincial Key Laboratory of Crop Germplasm Zhejiang UniversityHangzhou 310058China

Stomata play a crucial role in plants by controlling water status and responding to drought ***,simultaneously improving stomatal opening and drought tolerance has proven to be a significant *** address this issue,we employed the OnGuard quantitative model,which accurately represents the mechanics and coordination of ion transporters in guard *** the guidance of OnGuard,we successfully engineered plants that overexpressed the main tonoplast Ca^(2+)-ATPase gene,ACA11,which promotes stomatal opening and enhances plant ***,these transgenic plants also exhibited improved drought tolerance due to reduced water loss through their ***,OnGuard assisted us in understanding the mechanism behind the unexpected stomatal behaviors observed in the ACA11 overexpressing *** study revealed that the overexpression of ACA11 facilitated the accumulation of Ca^(2+)in the vacuole,thereby influencing Ca^(2+)storage and leading to an enhanced Ca^(2+)elevation in response to abscisic *** regulatory cascade finely tunes stomatal responses,ultimately leading to enhanced drought *** findings underscore the importance of tonoplast Ca^(2+)-ATPase in manipulating stomatal behavior and improving drought ***,these results highlight the diverse functions of tonoplast-localized ACA11 in response to different conditions,emphasizing its potential for future applications in plant enhancement.

关键词： ACA11 drought guard cells modeling stomata

Cryo-EM structure of cannabinoid receptor CB1-β-arrestincomplex

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Protein & Cell 2024年第3期15卷 230-234页

作者： yuxia Wang Lijie Wu Tian Wang Junlin Liu Fei Li Longquan Jiang Zhongbo Fan Yanan yu Na Chen Qianqian Sun qiwen Tan Tian Hua Zhi-Jie Liu iHuman Institute ShanghaiTech UniversityShanghai 201210China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Institute of Molecular and Clinical Medicine Kunming Medical UniversityKunming 650500China

Dear Editor,G protein-coupled receptors(GPCRs)play a vital role in regulating almost every aspect of human physiology,making up more than one-third of marketed drug targets(Santos et al.,2017).GPCRs orchestrate their signalling through interactions with three distinct downstream protein families:G proteins,G protein-coupled receptor kinases(GRKs),and arrestins(Santos et al.,2017).While G protein-mediated signalling is initiated upon GPCR stimulation,activated GPCRs return to their basal levels through a GRK-and arrestin-regulated desensitization process(Santos et al.,2017).In addition to modulating receptor desensitization,β-arrestin also regulates downstream events that are distinct from classical G protein signalling(Ahn et al.,2020).

关键词： stimulation return

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一种面向环境扫描的彩色点云流实时压缩算法

兵工学报 2023年第S2期44卷 167-177页

作者：马景起于脐文黄平王伟李友为哈尔滨工程大学智能科学与工程学院黑龙江哈尔滨150009 哈尔滨第一机械集团有限公司黑龙江哈尔滨150000

随着数字孪生技术的不断发展,人们面临将大规模、高维度的点云数据进行压缩编码的问题,以提高传输效率。然而大多点云编码方式存在压缩实时性不强、压缩效率低、点云格式要求过高等问题。针对解决这些问题,提出了一种基于谷歌Draco几何... 详细信息

随着数字孪生技术的不断发展,人们面临将大规模、高维度的点云数据进行压缩编码的问题,以提高传输效率。然而大多点云编码方式存在压缩实时性不强、压缩效率低、点云格式要求过高等问题。针对解决这些问题,提出了一种基于谷歌Draco几何压缩库的实时彩色点云流压缩(Real-time Color Stream Draco,RCS-Draco)算法。将算法集成到ROS框架内,借助ROS消息流,对点云流实时地进行编码和解码,提高了算法的实时性;通过建立优化裁剪模型,对点云进行裁剪和滤波,去除了漂移和离群点云,提高了压缩算法的压缩效率。建立量化预测模型,对点云的RGB颜色信息进行编码,解决了大多数点云压缩算法无法处理颜色信息的问题。对比试验通过调整压缩等级和量化参数,证明RCS-Draco算法的平均压缩率最高能够达到77%、平均压缩和解压时间小于0.035 s、位置平均误差小于0.05 m、属性平均误差小于35;并通过相融试验证明RCS-Draco算法在各项指标上优于Draco算法。试验结果表明,RCS-Draco压缩算法在压缩实时性、效率、点云格式方面均表现良好,能够有效地提升传输效率。

关键词：数字孪生压缩编码彩色点云流 ROS框架

Antigen-presenting effects of effector memory Vγ9Vδ2 T cells in rheumatoid arthritis

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Cellular & Molecular Immunology 2012年第3期9卷 245-254页

作者： Chaoying Hu Liu Qian Yi Miao Qiuyu Huang Ping Miao Ping Wang qiwen yu Hong Nie Jiying Zhang Dongyi He Rong Xu Xuehua Chen Bingya Liu Dongqing Zhang Shanghai Institute of Immunology Shanghai Jiao Tong University School of MedicineShanghaiChina Central laboratory Shanghai Xuhui Central HospitalShanghaiChina Guanghua Rheumatoid Arthritis Specialized Hospital ShanghaiChina Shanghai Jiao Tong University School of Medicine Ruijin HospitalShanghaiChina

Rheumatoid arthritis is an autoimmune disease that primarily affects the limbs, but the pathogenic mechanism remains unclear. 78 T cells, a T-cell subpopulation, are characterized by multiple biological functions and associated with a variety of diseases. This study investigated the antigen-presenting effects of γδ2 cells and their relationship with rheumatoid arthritis development. We found that Vγ9Vδ2 T cells （the predominant subtype of γδ T cells in peripheral blood） were activated by isopentenyl pyrophosphate to continuously proliferate and differentiate into effector memory cells. The effector memory Vγ9Vδ2 T cells exhibited phenotypic characteristics of specific antigen-presenting cells, including high HLA-DR and CD80/86 expression. These Vγ9Vδ2 T cells could present soluble antigens and synthetic peptides to CD4＋ T cells. Vγ9Vδ2 T cells with different phenotypes showed different cytokine secretion patterns. Effector memoryVγ9Vδ2 T cells simultaneously secreted not only interferon （IFN）-γbut also IL-17. The peripheral blood and joint synovial fluid from RA patients contained numerous heterogeneous γδ T cells that were predominantly effector memory Vγ9Vδ2 T cells with the ability to secrete inflammatory factors. We also found that γδ T cells had a similar antigen-presenting capability to B cells. These results suggest that during the development of rheumatoid arthritis, 78 T cells can aggravate immune dysfunction and produce abnormal immune damage by secreting cytokines and inducing inflammatory cells to participate in synergistic inflammatory responses. Furthermore, γδ T cells can behave similarly to B cells to present viral peptides and autoantigen peptides to CD4＋ T cells, thus sustaining CD4＋ T-cell activation.

关键词： antigen-presenting function rheumatoid arthritis γδT cell TEM Vγ9Vδ2 T cell

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方形光子晶体的全反射隧穿效应的滤波特性

压电与声光 2012年第3期34卷 351-354页

作者：刘启能代洪霞于奇文重庆工商大学废油资源化技术与装备教育部工程研究中心重庆400067 重庆工商大学计算机科学与信息工程学院重庆400067 辽阳鸿宇晶体有限公司辽宁辽阳111000

为了研究一维方形光子晶体中电磁波的全反射隧穿效应,利用传输矩阵法计算了电磁波在大于全反射角入射一维方形光子晶体的透射率。在透射波中发现了全反射隧穿效应。得出了方形光子晶体的全反射隧穿效应随模式量子数、边长、周期数的变... 详细信息

为了研究一维方形光子晶体中电磁波的全反射隧穿效应,利用传输矩阵法计算了电磁波在大于全反射角入射一维方形光子晶体的透射率。在透射波中发现了全反射隧穿效应。得出了方形光子晶体的全反射隧穿效应随模式量子数、边长、周期数的变化规律。为设计性能优良的光子晶体滤波器提供了理论依据。

关键词：方形光子晶体全反射隧穿效应滤波