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Cellular & Molecular Immunology 2018年第1期15卷 27-37页

作者： Yonglin Yu Lingtong Zhi qiuli wu Lina Jing Dayong Wang Key Laboratory of Developmental Genes and Human Diseases in Ministry of Education Medical SchoolSoutheast UniversityNanjing210009China

npr-9 encodes a homologue of the gastrin-releasing peptide receptor (GRPR) and is expressed in AIB interneurons. In this study, we investigated the role of NPR-9 in the neuronal control of innate immunity using the model system Caenorhabditis elegans. After exposure to Pseudomonas aeruginosa PA14, npr-9(tm1652) mutants showed resistance to infection, decreased PA14 colonization and increased expression of immunity-related genes. Nematodes overexpressing NPR-9 exhibited increased susceptibility to infection, increased PA14 colonization and reduced expression of immunity-related genes. In nematodes, ChR2-mediated AIB interneuron activation strengthened the innate immune response and decreased PA14 colonization. Overexpression of NPR-9 suppressed the innate immune response and increased PA14 colonization in nematodes with the activation of AIB interneurons mediated by ChR2 or by expressing pkc-1(gf) in AIB interneurons. We, therefore, hypothesize that NPR-9 regulates the innate immune response by antagonizing the activity of AIB interneurons. Furthermore, expression of GRPR, the human homologue of NPR-9, could largely mimic NPR-9 function by regulating innate immunity in nematodes. Our results provide insight into the pivotal role of interneurons in controlling innate immunity and the complex biological functions of GRPRs.

关键词： AIB interneuron Caenorhabditis elegans innate immunity neuronal basis NPR-9

Inhibition of ROS elevation and damage to mitochondrial function prevents lead-induced neurotoxic effects on structures and functions of AFD neurons in Caenorhabditis elegans

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Journal of Environmental Sciences 2012年第4期24卷 733-742页

作者： qiuli wu Peidang Liu Yinxia Li Min Du Xiaojuan Xing Dayong Wang Key Laboratory of Environmental Medicine Engineering in Ministry of Education Medical School of Southeast UniversityNanjing 210009China Department of Biochemistry and Molecular Biology Medical School of Southeast UniversityNanjing 210009China

Here we investigated the possible roles of oxidative stress in the formation of decreased thermotaxis to cultivation temperature in lead （Pb）-exposed nematodes Caenorhabditis elagans. Exposure to Pb at the examined concentrations decreased thermotaxis behaviors, and induced severe deficits in the structural properties of AFD sensory neurons. Meanwhile, Pb exposure caused the induction of severe oxidative damage, reactive oxygen species （ROS） production, and mitochondrial dysfunction in young adults. Moreover, pre-treatment with the antioxidants dimethyl sulfoxide （DMSO）, ascorbate and N-acetyl-L-cysteine （NAC）, used to inhibit both the ROS elevation and the mitochondrial dysfunction caused by Pb exposure, at the L2-1arval stage prevented the induction of oxidative damage and the formation of severe deficits in thermotaxis and structural properties of AFD sensory neurons in Pb-exposed young adults. Therefore, the formation of oxidative stress caused by Pb exposure may be due to both the induction of ROS elevation and damage to mitochondrial function, and oxidative stress may play a key role in inducing the neurotoxic effects on the structures and function of AFD sensory neurons in Pb-exposed nematodes.

关键词： thermotaxis lead exposure oxidative stress AFD sensory neuron Caenorhabditis elegans

X-irradiation for inhibiting glial scar formation in injured spinal cord

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Neural Regeneration Research 2013年第17期8卷 1582-1589页

作者： Guangzhi Ning Renhui Chen Yulin Li Qiang wu qiuli wu Yan Li Shiqing Feng Department of Orthopedics Tanjin Medical University General Hospital Department of Orthopedics Tianjin 4th Central Hospital

X-irradiation has a beneficial effect in treating spinal cord injury. We supposed that X-irradiation could improve the microenvironment at the site of a spinal cord injury and inhibit glial scar formation. Thus, this study was designed to observe the effects of 8 Gy X-irradiation on the injury site at 6 hours and 2, 4, 7, and 14 days post injury, in terms of improvement in the microenvironment and hind limb motor function. Immunohistochemistry showed that the expression of macrophage marker ED-1 and the area with glial scar formation were reduced. In addition, the Basso, Beattie and Bresnahan score was higher at 7 days post injury relative to the other time points post injury. Results indicated that X-irradiation at a dose of 8 Gy can inhibit glial scar formation and alleviate the inflammatory reaction, thereby repairing spinal cord injury. X-irradiation at 7 days post spinal cord injury may be the best time window.

关键词： neural regeneration spinal cord glial scar X-irradiation functional recovery astrocytes grants-supported paper neuroregeneration

Molecular control of TiO2-NPs toxicity formation at predicted environmental relevant concentrations by Mn-SODs proteins in nematode Caenorhabditis elegans

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Molecular control of TiO2-NPs toxicity formation at predicte...

统计学实验设计分析颗粒状TiO_2光催化AB7染料废水

2014线粒体毒性与基于毒性通路的安全性评价新策略学术研讨会暨中国毒理学会毒理学替代法与转化毒理学专业委员会成立大会

作者： Yinxia Li qiuli wu Dayong Wang Medical School of Southeast University

With growing concems of the safety of nanotechnology,the in vivo toxicity of nanoparticles(NPs)at environmental relevant concentrations has drawn increasing *** investigated the possible molecular mechanisms of titanium nanoparticles(TiO-NPs)in the induction of toxicity at predicted environmental relevant *** nematodes,small sizes(4 nm and 10 nm)of TiO-NPs

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太阳能学报 2008年第2期29卷 152-157页

作者：傅剑锋武秋立东南大学能源与环境学院南京210096 天津大学化工学院天津300072

利用颗粒状纳米TiO_2在间歇光反应器中对酸性湖蓝光催化氧化脱色进行了研究,并根据统计学的响应面方法对光催化氧化酸性湖蓝的主要因素(初始pH值、光强、TiO_2浓度)进行了探讨和分析。为了得到这3个因素之间的相互关系并优化这些参数,... 详细信息

利用颗粒状纳米TiO_2在间歇光反应器中对酸性湖蓝光催化氧化脱色进行了研究,并根据统计学的响应面方法对光催化氧化酸性湖蓝的主要因素(初始pH值、光强、TiO_2浓度)进行了探讨和分析。为了得到这3个因素之间的相互关系并优化这些参数,实验采用Box-Behnken设计方法,其中初始pH值、光强、TiO_2浓度的高、低水平分别为3.3～5.7,11.11～20.11×10~2μW/cm^2,0.4～1.4mg/L,分析参数为脱色率的变化。通过使用Design-Expert 5软件可得到1个2次响应曲面模型,并得到最佳的初始pH值、光强、TiO_2浓度分别为3.46、16.50×10~2μW/cm^2、0.98mg/L,从而脱色率也达到最大(98.82%)。

关键词：酸性湖蓝光催化颗粒状纳米TiO2 脱色率响应面方法 Box-Behnken设计

NPR-9 Regulates Innate Immunity by Antagonizing Functions of AIB Interneurons in Caenorhabditis elegans

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NPR-9 Regulates Innate Immunity by Antagonizing Functions of...

Research Progress on Rapid Breeding Technology of Anoectochilus roxburghii Seedlings

第九届全国免疫学学术大会

作者： Lina Jing qiuli wu Lingmei Sun Dayong Wang Southeast University

Background:In human beings,mutations of GRPR gene are associated with the occurrence of several diseases,such as ***-9 is the homologue of human GPPR in Caenorhabditis ***:We investigated neuronal control of innate immunity by NPR-9 in Caenorhabditis ***:*** PA14 pathogenesis assay,lifespan assay,behavioral analysis,and *** bacterial CFUanalysis were performed in this *** real-time polymerase chain reaction(PCR)was used to assess the transcriptional expression of ten immunity-related genes with antimicrobial *** further assess the physiological state of AIB intemeurons in nematodes exposed to *** PAH,we expressed the Ca-sensitive fluorescent protein G-CaMP2.0 in AIB interneurons and measured the calcium levels under various ***,we performed the optogenetic manipulations of AIB ***:After exposure to Pseudomonas aeruginosa PAH,npr-9(tm1652)mutants showed resistance to infection,decreased PAH colonization in intestine,and higher expression of immunity-related genes,whereas nematodes overexpressing NPR-9 exhibited a susceptible property to infection,increased PAH colonization in intestine,and lower expression immunity-related *** of AIB intemeurons could be activated by PAH,and further enhanced by npr-9 *** nematodes,ChR2-mediated activation of AIB intemeurons strengthened innate immune and decreased PAH colonization in intestine;however,ARCH-mediated silencing of AIB intemeurons suppressed innate immune and increased PAH colonization in ***,after exposure to PAH,overexpression of NPR-9 suppressed innate immunity and increased PAH colonization in intestine of nematodes with activation of AIB interneurons mediated by ChR2 or expressing pkc-1(gf)in AIB ***,we hypothesize that NPR-9 may antagonize the functions of AIB intemeurons to regulate innate immune in ***,expression of human GRPR could largely mimic t

关键词： NPR-9 GRPR AIB interneuron neuronal basis innate immunity Caenorhabditis elegans

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Medicinal Plant 2018年第1期9卷 39-42,46页

作者： Qinger JIANG Xiaojing TAN qiuli wu Mei wu Xiaoxia SHEN Zhonghua WANG Institute of Biotechnology Zhejiang Wanli University Jinhua Jinglong Biological Technology Co.Ltd. Zhejiang Institute of Chinese Meteria Medica

As a traditional precious Chinese herbal medicine,Anoectochilus roxburghii has the cooling and detoxifying effects,and can nourish yin to reduce pathogenic fire,and has anti-inflammatory and analgesic effects. In this paper,we summarized the research progress in detail about the biological characteristics,resource distribution,composition,medicinal value and rapid seedling breeding technology concerning the rare Chinese herbal medicine A. roxburghii,in order to provide a reference for the in-depth study and rational development and utilization of ***.

关键词： Anoectochilus roxburghii Germplasm resources Medicinal value Rapid breeding technology Research progress

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羊肚菌胞外多糖发酵动力学模型

南开大学学报（自然科学版） 2005年第1期38卷 43-48页

作者：武秋立安家彦天津大学化工学院天津300072 大连轻工业学院生物与食品工程学院辽宁大连116034

对羊肚菌胞外多糖发酵动力学进行了研究.基于Logistic方程和Luedeking-Piret方程,得到了描述羊肚菌发酵过程菌体生长、胞外多糖生成和底物消耗的动力学数学模型和模型参数.同时对试验数据与模型进行了验证比较,模型计算值与试验结果... 详细信息

对羊肚菌胞外多糖发酵动力学进行了研究.基于Logistic方程和Luedeking-Piret方程,得到了描述羊肚菌发酵过程菌体生长、胞外多糖生成和底物消耗的动力学数学模型和模型参数.同时对试验数据与模型进行了验证比较,模型计算值与试验结果拟合良好,平均相对误差小于10％.

关键词：羊肚菌胞外多糖发酵动力学模型 Logistic方程 Luedeking-Piret方程

响应面法分析Fenton氧化垃圾渗滤液的过程

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化工进展 2006年第12期25卷 1493-1495,1503页

作者：傅剑锋武秋立东南大学能源与环境学院江苏南京210096 天津大学化工学院天津300072

利用统计学的方法对Fenton氧化垃圾渗滤液的影响因素进行了探讨和分析,考察了FeSO4?7H2O投加量,H2O2/FeSO4.7H2O比值和pH值对氧化垃圾渗滤液的影响。在FeSO4.7H2O投加量为0.01～0.02 mol/L、H2O2/FeSO4.7H2O比值1～5和pH值2～6的条件下... 详细信息

利用统计学的方法对Fenton氧化垃圾渗滤液的影响因素进行了探讨和分析,考察了FeSO4?7H2O投加量,H2O2/FeSO4.7H2O比值和pH值对氧化垃圾渗滤液的影响。在FeSO4.7H2O投加量为0.01～0.02 mol/L、H2O2/FeSO4.7H2O比值1～5和pH值2～6的条件下,分析CODCr的变化。通过使用Design-Expert 5软件可得到1个二次响应曲面模型,最佳的FeSO4.7H2O投加量为0.013mol/L、H2O2/FeSO4.7H2O比值4.60和pH值4.45,从而CODCr的去除率也达到最大(69.85%)。

关键词：垃圾渗滤液 Fenton氧化统计学法响应面方法

环境暴露致秀丽线虫衰老毒性及相关作用机制研究进展

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环境污染与防治 2011年第10期33卷 73-78页

作者：武秋立王大勇东南大学医学院江苏南京210009

衰老作为一个重要的评价终点,目前已经广泛应用于秀丽线虫环境暴露评价以及相关毒理学研究。系统总结了各种金属以及有机污染物暴露所致秀丽线虫衰老毒性;分析了胰岛素信号、应激反应与氧化应激、线粒体呼吸链、金属硫蛋白、金属诱导胁... 详细信息

衰老作为一个重要的评价终点,目前已经广泛应用于秀丽线虫环境暴露评价以及相关毒理学研究。系统总结了各种金属以及有机污染物暴露所致秀丽线虫衰老毒性;分析了胰岛素信号、应激反应与氧化应激、线粒体呼吸链、金属硫蛋白、金属诱导胁迫响应蛋白NUMR-1和NUMR-2等在环境暴露致秀丽线虫衰老毒性形成中的重要调控功能;指出了代谢变化与环境暴露致秀丽线虫衰老毒性形成之间存在的密切关联;讨论了基于环境暴露致秀丽线虫衰老毒性及相应毒作用机制研究中尚存在的问题以及可能的解决策略。

关键词：衰老环境暴露毒性评价毒害机制秀丽线虫