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集中岗前技能强化培训在临床医学专业本科生儿科临床实习中的作用

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中华妇幼临床医学杂志（电子版） 2015年第2期11卷 92-95页

作者：朱霞张玉洁张趁张伟令阴伟郭秋菊张谊胡惠敏黄东生首都医科大学附属北京同仁医院儿科 100730 首都医科大学附属北京同仁医院教育处 100730

目的探讨集中岗前技能强化培训在医学本科生儿科临床实习中的作用。方法选择在首都医科大学附属北京同仁医院实习的120例2008级及60例2005级临床医学专业本科生为研究对象。按照随机数字表法将2008级医学生分为2008级实验组及2008级对照... 详细信息

目的探讨集中岗前技能强化培训在医学本科生儿科临床实习中的作用。方法选择在首都医科大学附属北京同仁医院实习的120例2008级及60例2005级临床医学专业本科生为研究对象。按照随机数字表法将2008级医学生分为2008级实验组及2008级对照组,每组各60例;将2005级60例医学生则纳入2005级对照组。对实验组学生在实习前进行7d集中儿科技能强化培训,而对照组则未进行强化培训。强化培训内容包括儿科体格检查、小儿骨髓细胞学穿刺术、腰椎穿刺术、小儿头皮静脉穿刺术及婴儿配奶,并对2008级学生在实习结束时,进行《儿科技能培训问卷调查表》调查,内容包括儿科技能培训必要性,提高儿科技能培训兴趣,提高儿科实习兴趣,提高儿科疾病知识及教具使用满意率。分别比较2008级实验组与2008级对照组及2005级对照组学生实习结束时,儿科技能考核评分及优秀率差异,并对问卷调查结果进行统计学分析。3组学生性别、年龄、实习前成绩比较,差异均无统计学意义(P>0.05)。所有学生均自愿参加本次研究。结果 2008级实验组学生儿内科出科技能考核评分及优秀率,均显著高于2008级对照组及2005级对照组学生,且差异有统计学意义(P0.05)。结论实习前儿科技能强化培训,可加深学生对儿科临床疾病知识的理解,并熟练儿内科常用操作,增强动手能力,提高儿科实习兴趣及效率。

关键词：强化培训临床工作能力临床实习

舒肝化癥方对肝纤维化模型大鼠VEGF、CTGF、CD31及vWF表达的影响及其相关性研究

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中医药信息 2024年第6期41卷 19-27页

作者：邢安丽赵鲲鹏张秋菊李婕妠陈世玉郭嘉琪张铭甘肃中医药大学甘肃兰州730000

目的:以四氯化碳(CCl_(4))致大鼠肝纤维化为研究对象,研究舒肝化癥方(SGHZD)对大鼠肝纤维化血瘀证的影响及机理,分析血管内皮生长因子(VEGF)、结缔组织生长因子(CTGF)、血小板-内皮细胞黏附分子(CD31)及血管性血友病因子(vWF)在SGHZD抗... 详细信息

目的:以四氯化碳(CCl_(4))致大鼠肝纤维化为研究对象,研究舒肝化癥方(SGHZD)对大鼠肝纤维化血瘀证的影响及机理,分析血管内皮生长因子(VEGF)、结缔组织生长因子(CTGF)、血小板-内皮细胞黏附分子(CD31)及血管性血友病因子(vWF)在SGHZD抗肝纤维化过程中的表达及其相关性。方法:SPF级SD大鼠,雄性,54只,按随机原则分成空白组、模型组(2.0 mg/kg 40%CCl_(4)橄榄油溶液+等量的0.9%的生理盐水)、阳性对照组(2.0 mg/kg 40%CCl_(4)橄榄油溶液+0.2 mg/kg秋水仙碱溶液)、SGHZD高剂量组(2.0 mg/kg 40%CCl_(4)橄榄油溶液+29.52g/kgSGHZD)、SGHZD中剂量组(2.0 mg/kg 40%CCl_(4)橄榄油溶液+14.76 g/kgSGHZD)和SGHZD低剂量组(2.0 mg/kg 40%CCl_(4)橄榄油溶液+7.38 g/kgSGHZD),各9只。造模时间为8周,每周2次,从造模第2天起,用药治疗每天1次,治疗8周,实验期间动态监测所有大鼠的生理情况,马松染色(Masson)对肝组织病理形态学变化和胶原纤维沉积情况进行观察,实时荧光定量聚合酶链式反应(Real-time PCR)检测VEGF、CTGF、CD31、vWF mRNA的表达水平,Western blot检测VEGF,CTGF,CD31,vWF的蛋白表达。结果:与空白组相比,模型组大鼠整体状况明显下降,Masson染色结果显示大鼠汇管区和中央静脉区形成假小叶,并伴有纤维间隔形成,胶原纤维沉积增多且有显著意义(P<0.05);RT-qPCR、Western blot结果显示,VEGF、CTGF、CD31、vWF mRNA和蛋白表达均上升(P<0.05);与模型组相比,阳性对照组和SGHZD组各剂量Masson染色显示胶原纤维沉积明显减少,纤维间隔程度减轻,差异有统计学意义(P<0.05),RT-qPCR、Western blot结果显示VEGF、CTGF、CD31、vWF mRNA及蛋白的表达量均下降,但以阳性对照组及中药高剂量组效果最好(P均<0.05);将Masson染色胶原容积分数分别与VEGF、CTGF、CD31及vWF蛋白表达进行相关性分析,发现VEGF、CTGF、CD31及vWF在肝纤维化的形成过程中随着肝纤维化程度加重其表达上调,而在SGHZD抗肝纤维化过程中随着胶原容积分数减少其表达减少;对VEGF、CTGF、CD31及vWF蛋白表达进行相关性分析,发现VEGF、CTGF、CD31及vWF在肝纤维化的形成发展中和SGHZD抗肝纤维化的过程中均呈显著正相关。结论:SGHZD对CCl_(4)所致肝纤维化大鼠有显著疗效,其作用机理可能与VEGF、CTGF、CD31及vWF的表达、病理性血管生成、肝窦毛细血管化及肝纤维化血瘀证相关。

关键词：血管内皮生长因子结缔组织生长因子血小板-内皮细胞黏附分子血管性血友病因子肝纤维化舒肝化癥方

Genetic mapping of folate QTLs using a segregated population in maizeFA

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Journal of Integrative Plant Biology 2019年第6期61卷 675-690页

作者： Wenzhu guo Tong Lian Baobao Wang Jiantao Guan Dong Yuan Huan Wang Fardous Mohammad Safiul Azam Xing Wan Weixuan Wang qiuju Liang Haiyang Wang Jinxing Tu Chunyi Zhang Ling Jiang College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Plant Genetics Gembloux Agro-Bio TechUniversity of LiegeGembloux 5030Belgium Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Department of Chemistry and Chemical Engineering Qilu Normal UniversityJinan 250200China School of Chemistry and Molecular Engineering East China University of Science and TechnologyShanghai 200237China

As essential B vitamin for humans, folates accumulation in edible parts of crops, such as maize kernels, Is of great importance for human health. But its breeding is always limited by the prohibitive cost of folate profiling. The molecular breeding is a more executable and efficient way for folate fortification, but is limited by the molecular knowledge of folate regulation. Here we report the genetic mapping of folate quantitative trait loci (QTLs) using a segregated population crossed by two maize lines, one high in folate (GEMS31) and the other low in folate (DAN3130). Two folate QTLs on chromosome 5 were obtained by the combination of F2 whole-exome sequencing and F3 kernel-folate profiling. These two QTLs had been confirmed by bulk segregant analysis using F6 pooled DNA and F7 kernel-folate profiling, and were overlapped with QTLs identified by another segregated population. These two QTLs contributed 41.6% of phenotypic variation of 5-formyltetrahydrofolate, the most abundant storage form among folate derivatives in dry maize grains, in the GEMS31×DAN3130 population. Their fine mapping and functional analysis will reveal details of folate metabolism, and provide a basis for marker-assisted breeding aimed at the enrichment of folates in maize kernels.

关键词： Genetic mapping folate QTLs using a segregated population in maize^FA

miR-7/TGF-β2 axis sustains acidic tumor microenvironment-induced lung cancer metastasis

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Acta Pharmaceutica Sinica B 2022年第2期12卷 821-837页

作者： Tao Su Suchao Huang Yanmin Zhang Yajuan guo Shuwei Zhang Jiaji Guan Mingjing Meng Linxin Liu Caiyan Wang Dihua Yu Hiu-Yee Kwan Zhiying Huang qiuju Huang Elaine Lai-Han Leung Ming Hu Ying Wang Zhongqiu Liu Linlin Lu Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People’s Republic of China Guangzhou University of Chinese MedicineGuangzhou 510006China Department of Molecular and Cellular Oncology the University of Texas MD Anderson Cancer CenterHoustonTX 77030USA School of Chinese Medicine Hong Kong Baptist UniversityHong Kong 999077China State Key Laboratory of Quality Research in Chinese Medicine/Macao Institute for Applied Research in Medicine and Health Macao University of Science and TechnologyMacao(SAR)999078China Department of Pharmacological and Pharmaceutical Sciences University of HoustonHoustonTX 77204USA

Acidosis,regardless of hypoxia involvement,is recognized as a chronic and harsh tumor microenvironment(TME)that educates malignant cells to thrive and *** overwhelming evidence supports an acidic environment as a driver or ubiquitous hallmark of cancer progression,the unrevealed core mechanisms underlying the direct effect of acidification on tumorigenesis have hindered the discovery of novel therapeutic targets and clinical ***,chemical-induced and transgenic mouse models for colon,liver and lung cancer were established,***-7 and TGF-β2 expressions were examined in clinical tissues(n=184).RNA-seq,miRNA-seq,proteomics,biosynthesis analyses and functional studies were performed to validate the mechanisms involved in the acidic TME-induced lung cancer *** data show that lung cancer is sensitive to the increased acidification of TME,and acidic TME-induced lung cancer metastasis via inhibition of miR-7-5 ***-β2 is a direct target of miR-7-5 *** reduced expression of miR-7-5 p subsequently increases the expression of TGF-β2 which enhances the metastatic potential of the lung ***,overexpression of miR-7-5 p reduces the acidic p H-enhanced lung cancer ***,the human lung tumor samples also show a reduced miR-7-5 p expression but an elevated level of activated TGF-β2;the expressions of both miR-7-5 p and TGF-β2 are correlated with patients’*** are the first to identify the role of the miR-7/TGF-β2 axis in acidic p H-enhanced lung cancer *** study not only delineates how acidification directly affects tumorigenesis,but also suggests miR-7 is a novel reliable biomarker for acidic TME and a novel therapeutic target for non-small cell lung cancer(NSCLC)*** study opens an avenue to explore the p H-sensitive subcellular components as novel therapeutic targets for cancer treatment.

关键词： Acidic tumor microenvironment miR-7-5p TGF-β2 Metastasis Lung cancer pH Invasion

The Motion Simulation of Human Lower Extremity Based on Dynamics

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The Motion Simulation of Human Lower Extremity Based on Dyna...

Oral microbiome and risk of malignant esophageal lesions in a high-risk area of China:A nested case-control study

第七届亚太地区医学与生物学工程学术年会

作者： Jin Li qiuju Cheng Hong Liang Zhuowei guo Automation College Harbin Engineering University Harbin China

Based on the knowledge of Human Anatomy and the multi-body dynamics, a simplified model of the human lower extremity is firstly established using the ADAMS in this paper. In process of modeling for lower extremity, the lower extremity is simplified as thighbone and shankbone joined by knee joint for the patella is very small. There is anther as-sumption that the femur and tibia are simulated as cylindrical rigid body for their shape, which is similar to cylinder. Then, the motion of flexion-extension and medial or lateral rotation of Human’s thigh and lower leg’s flexion-extension motion is simulated in this paper. In addition, the muscles which are related to the flexion-extension motion are added to the model in the form of point-point force. Lastly, several general move-ments of muscles-bones of human lower extremity are simu-lated for researching the principles of force and torque of every joint in process of movement using the kinematics and dynamics analysis function of ADAMS. The results of the simulation are analyzed as well in this paper.

关键词： ADAMS muscular model lower extremity point-point force motion simulation

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Chinese Journal of Cancer Research 2020年第6期32卷 742-754页

作者： Fangfang Liu Mengfei Liu Ying Liu Chuanhai guo Yunlai Zhou Fenglei Li Ruiping Xu Zhen Liu qiuju Deng Xiang Li Chaoting Zhang Yaqi Pan Tao Ning Xiao Dong Zhe Hu Huanyu Bao Hong Cai Isabel Dos Santos Silva Zhonghu He Yang Ke Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Laboratory of GeneticsPeking University Cancer Hospital&InstituteBeijing 100142China Novogene Co. LtdBeijing 100080China Hua County People's Hospital Anyang 456400China Anyang Cancer Hospital Anyang 455000China Department of Non-communicable Disease Epidemiology London School of Hygiene&Tropical MedicineLondon WC1E 7HTUK

Objective:We aimed to prospectively evaluate the association of oral microbiome with malignant esophageal lesions and its predictive potential as a biomarker of ***:We conducted a case-control study nested within a population-based cohort with up to 8 visits of oral swab collection for each subject over an 11-year period in a high-risk area for esophageal cancer in *** oral microbiome was evaluated with 16 S ribosomal RNA(rRNA)gene sequencing in 428 pre-diagnostic oral specimens from 84 cases with esophageal lesions of severe squamous dysplasia and above(SDA)and 168 matched healthy *** analysis was performed to identify taxa of differential *** oral species together with subject characteristics were evaluated for their potential in predicting SDA risk by constructing conditional logistic regression ***:A total of 125 taxa including 37 named species showed significantly different abundance between SDA cases and controls(all P***:The oral microbiome may play an etiological and predictive role in esophageal cancer,and it holds promise as a non-invasive early warning biomarker for risk stratification for esophageal cancer screening programs.

关键词： Early warning biomarker esophageal squamous cell carcinoma oral microbiome risk prediction

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MOOC学习投入度与学习坚持性关系研究

开放教育研究 2019年第2期25卷 65-77页

作者：兰国帅郭倩钟秋菊河南大学教育科学学院河南开封475004 河南省教育信息化发展研究中心河南开封475004 河南大学技术促进学习创新研究院河南开封475004 北京师范大学教育学部北京100875

MOOC已成为我国远程教育领域的研究热点之一,然而如何设计、开发和实施基于MOOC的混合教学,提高MOOC学习者的学习投入度与学习坚持性,促进学习者有意义的深度学习,是利用MOOC实施混合教学需解决的关键问题。本研究从学习者、教师和学习... 详细信息

MOOC已成为我国远程教育领域的研究热点之一,然而如何设计、开发和实施基于MOOC的混合教学,提高MOOC学习者的学习投入度与学习坚持性,促进学习者有意义的深度学习,是利用MOOC实施混合教学需解决的关键问题。本研究从学习者、教师和学习支持系统融合的角度,以探究学习社区中文量表为研究工具,探究MOOC学习者的学术自我效能感、教学存在感、感知有用性、感知易用性对其学习投入度与学习坚持性的影响,并进一步探讨如何提升MOOC学习者的学习投入度与坚持性。研究表明:MOOC学习者的学术自我效能感、教学存在感和感知有用性对其学习投入度有显著的正向直接影响;MOOC学习者的学习投入度和感知易用性对其学习坚持性有显著的正向直接影响;MOOC学习者的学习投入度对其学术自我效能感、教学存在感、感知有用性与学习坚持性之间的关系起中介效应。这些发现可为设计有效的MOOC教学和学习策略,以及在网络学习空间中有效开展基于MOOC的混合教学实践和研究提供新视野。

关键词：学习投入度学习坚持性学术自我效能感教学存在感感知易用性和有用性网络学习空间

网络聚合与集体欢腾:国庆阅兵仪式如何影响青年群体集体记忆建构

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旅游学刊 2021年第8期36卷 127-139页

作者：郭云娇陈斐罗秋菊中山大学旅游学院广东广州510275 中山大学旅游发展与规划研究中心广东广州510275

青年群体是新时代爱国主义情感培育的关键对象。中华人民共和国70年国庆阅兵仪式作为建构集体记忆的重要媒介,对青年人的国家认同感与爱国主义情感培养发挥着积极的作用。研究立足于社交平台"豆瓣"中一个最大规模的线上讨论小组"豆瓣鹅... 详细信息

青年群体是新时代爱国主义情感培育的关键对象。中华人民共和国70年国庆阅兵仪式作为建构集体记忆的重要媒介,对青年人的国家认同感与爱国主义情感培养发挥着积极的作用。研究立足于社交平台"豆瓣"中一个最大规模的线上讨论小组"豆瓣鹅组",将网络社群中的青年群体作为主要研究对象,通过网络文本数据爬取和半结构访谈相结合的方法,探究阅兵仪式在国家集体记忆建构中的媒介作用,阐释从个体记忆唤醒到集体记忆生成的动态过程、要素与特征,并完善仪式建构集体记忆的链条模型。研究发现:(1)国家庆典仪式对个体记忆唤醒是由外部提醒和内部驱动两方面因素共同促成。(2)国家庆典仪式对群体的集体记忆的建构过程呈现出当下性、结构性和群体性的特征,由仪式建构的集体记忆对个体的影响主要体现在认知、情感和行为3个层面,具体表现为个体的国民意识、家国情怀和爱国行动3个方面。文章既以中国本土化的大型节庆仪式研究深化了仪式的集体记忆功能与理论内涵,也为网络社群时代强化国家庆典仪式的爱国情感培育功能提供可操作的实践方式与思路。

关键词：国家庆典仪式集体记忆爱国情感豆瓣鹅组

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基于自注意力网络的共享账户跨域序列推荐

计算机研究与发展 2021年第11期58卷 2524-2537页

作者：郭磊李秋菊刘方爱王新华山东师范大学商学院济南250358 山东师范大学信息科学与工程学院济南250358

共享账户跨域序列推荐(shared-account cross-domain sequential recommendation,SCSR)是指在多个用户共同使用一个账户和用户的行为会在多个域中产生的情况下,给该账户推荐下一个可能会点击的项目.与传统的序列推荐任务相比,共享账户... 详细信息

共享账户跨域序列推荐(shared-account cross-domain sequential recommendation,SCSR)是指在多个用户共同使用一个账户和用户的行为会在多个域中产生的情况下,给该账户推荐下一个可能会点击的项目.与传统的序列推荐任务相比,共享账户跨域序列推荐主要面临2方面的挑战:1)每一个账户里的交互行为是由多个用户产生的,并且这些用户的行为是混合在一起的;2)用户在1个域中产生的交互行为可能会提升推荐系统对该用户在其他域的推荐质量.目前,已有的一些相关工作大都是基于循环神经网络(recurrent neural network,RNN)的方法,但由于RNN本身固有的一些缺陷,导致基于RNN的方法不仅非常耗时,而且不能够很好地捕获交互行为之间的长期依赖关系.针对SCSR问题,提出了基于自注意力的跨域推荐模型(self-attention-based cross-domain recommendation model,SCRM)来解决这2个挑战.具体而言,首先引入1个多头自注意力网络来建模1个共享账户中多个用户参与的交互行为.然后,提出了一个基于多层交叉映射感知网络的跨域传输单元,以实现借助一个域的信息来提高另一个域的推荐质量.最后,通过一个混合推荐解码器整合了来自2个域的信息以实现在不同域中的推荐.在真实数据集HVIDEO上进行了实验,实验结果表明,与目前最新的基准方法相比,所提出的模型能在MRR和Recall这2个指标上取得了更加优异的结果;在运行效率上,比基于RNN的方法取得了更短的训练和学习时间.

关键词：多头自注意力共享账户推荐跨域推荐序列建模协同过滤