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ANXA2 Facilitates Enterovirus 71 Infection by Interacting with 3D Polymerase and PI4KB to Assist the Assembly of Replication Organelles

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Virologica Sinica 2021年第6期36卷 1387-1399页

作者： qiuhan zhang Siliang Li Ping Lei Zixian Li Feifei Chen Qi Chen Yulu Wang Jiami Gong Qi Tang Xinjin Liu Ke Lan Shuwen Wu State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan430072China Medical Research Institute Wuhan UniversityWuhan430072China Frontier Science Center for Immunology and Metabolism Wuhan UniversityWuhan430072China

Similar to that of other enteroviruses, the replication of enterovirus 71(EV71) occurs on rearranged membranous structures called replication organelles(ROs). Phosphatidylinositol 4-kinase Ⅲ(PI4KB), which is required by enteroviruses for RO formation, yields phosphatidylinositol-4-phosphate(PI4P) on ROs. PI4P then binds and induces conformational changes in the RNA-dependent RNA polymerase(Rd Rp) to modulate Rd Rp activity. Here, we targeted 3D polymerase, the core enzyme of EV71 ROs, and found that the host factor Annexin A2(ANXA2) can interact with 3 D polymerase and promote the replication of EV71. Then, an experiment showed that the annexin domain of ANXA2, which possesses membranebinding capacity, mediates the interaction of ANXA2 with EV71 3 D polymerase. Further research showed that ANXA2 is localized on ROs and interacts with PI4KB. Overexpression of ANXA2 stimulated the formation of PI4P, and the level of PI4P was decreased in ANXA2-knockout cells. Furthermore, ANXA2, PI4KB, and 3D were shown to be localized to the viral RNA replication site, where they form a higher-order protein complex, and the presence of ANXA2 promoted the PI4 KB-3D interaction. Altogether, our data provide new insight into the role of ANXA2 in facilitating formation of the EV71 RNA replication complex.

关键词： Enterovirus 71(EV71) Annexin A2(ANXA2) 3D polymerase Phosphatidylinositol 4-kinaseⅢ(PI4KB)

Current AAV-mediated gene therapy in sensorineural hearing loss

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Fundamental Research 2025年第1期5.0卷 192-202页

作者： Qi, Jieyu Fu, Xiaolong zhang, Liyan Tan, Fangzhi Li, Nianci Sun, qiuhan Hu, Xiaojie He, Zuhong Xia, Ming Chai, Renjie State Key Laboratory of Bioelectronics Department of Otolaryngology Head and Neck Surgery Zhongda Hospital School of Life Sciences and Technology Advanced Institute for Life and Health Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research Southeast University Nanjing 210096 China Department of Otorhinolaryngology-Head and Neck Surgery Zhongnan Hospital of Wuhan University Wuhan 430071 China Department of Otolaryngology Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan 250021 China Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan 250021 China Co-Innovation Center of Neuroregeneration Nantong University Nantong 226001 China Institute for Stem Cell and Regeneration Chinese Academy of Science Beijing 100101 China Beijing Key Laboratory of Neural Regeneration and Repair Capital Medical University Beijing 100069 China Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu 610072 China

The number of patients with hearing loss is on the rise due to congenital abnormalities, degenerative changes in old age, and acquired injuries such as virus or ototoxic drug-induced diseases. Hearing loss is a refractory and disabling disease that has serious negative effects on quality of life. The pathology of hearing loss in the inner ear is characterized by varying degrees of damage to the cochlear sensory epithelium cells (such as hair cells and supporting cells), stria vascularis (including marginal, intermediate and basal cells) and spiral ganglion neurons. Regeneration or direct repair of damaged cells in the inner ear is an effective way to treat sensorineural deafness. It is currently possible to regenerate hair cells to treat sensorineural hearing loss by FX-322, a small molecule drug in clinical trials. With the development of genetic engineering technology, gene therapy has brought a promising treatment strategy for many previously intractable diseases. Gene therapy has been regarded as a promising method in the treatment and rehabilitation of sensorineural hearing loss, and recombinant adeno-associated virus gene therapy has been widely used in fundamental research into hearing loss treatments. At present, gene therapy for hearing loss is transitioning from feasibility studies to explorations of its safety and its therapeutic potential. The present article reviews the concepts, strategies, and applications of gene therapy mediated by recombinant adeno-associated viruses in the field of hearing loss treatment. © 2022

关键词： Adeno-associated virus Auditory repair Cochlea Gene therapy Hearing loss

Dyngo-4a protects mice from rotavirus infection by affecting the formation of dynamin 2 oligomers

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Science Bulletin 2020年第21期65卷 1796-1799,M0003页

作者： Quanzhi zhang qiuhan zhang Zhichao Xu Qi Tang Xinjin Liu Danping Niu Xiaoming Gao Ke Lan Shuwen Wu Institute of Biology and Medical Sciences Soochow UniversitySoochow 215006China State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan 430072China State Key Laboratory of Virology Key Laboratory of Combinatorial Biosynthesis and Drug DiscoveryMinistry of EducationSchool of Pharmaceutical SciencesWuhan UniversityWuhan 430071China

Rotavirus is the most common etiologic agent of severe diarrhea in infants and young children and causes more than 200,000 deaths annually worldwide[1,2].Licensed rotavirus vaccines can provide more than 50%protection against rotavirus infection,and currently available etiological treatments for rotavirus gastroenteritis mainly involve the use of oral rehydration solution and zinc supplementation[3].However,targeted interventions are in great need for rotavirus-induced gastroenteritis control.

关键词：轮状病毒腹泻轮状病毒疫苗轮状病毒感染细胞病变寡聚体药物筛选治疗手段

应用Matlab软件对架空输电线路覆冰舞动故障的分析

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内蒙古电力技术 2016年第2期34卷 10-13页

作者：胡坤岐张秋晗国网北京经济技术研究院徐州勘测设计中心江苏徐州221000 徐州华电电力勘察设计有限公司江苏徐州221000

分析了导线覆冰舞动的形成因素,以扇形覆冰导线为例,应用Matlab软件对扇形覆冰导线在不同风速、攻角以及档距条件下导线的振幅相平面图进行数值模拟分析,得到覆冰导线在不同参数条件下发生舞动的时程曲线,并得出相应结论。通过对导线覆... 详细信息

分析了导线覆冰舞动的形成因素,以扇形覆冰导线为例,应用Matlab软件对扇形覆冰导线在不同风速、攻角以及档距条件下导线的振幅相平面图进行数值模拟分析,得到覆冰导线在不同参数条件下发生舞动的时程曲线,并得出相应结论。通过对导线覆冰舞动机理及舞动特性进行分析与研究,对输电线路的安全稳定运行提供重要的理论依据。

关键词：架空输电线路导线覆冰舞动 Matlab 数值模拟时程曲线风速攻角档距

亏祖山秋季不同海拔土壤纤毛虫优势种生态位及其与环境因子的关系

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生态学报 2022年第9期42卷 3494-3503页

作者：王壮壮朱时应张秋涵何梦萨普布西藏大学理学院生命科学系高原动物学实验室拉萨850000

为深入了解拉萨河流域亏祖山不同海拔梯度秋季土壤纤毛虫优势种生态位及其与环境因子的关系,于2015年10月在亏祖山共设置11个样点,用土壤采样器采用梅花五点式采集土样,同时记录相应的理化因子,土样在实验室自然风干后,采用“非淹没培... 详细信息

为深入了解拉萨河流域亏祖山不同海拔梯度秋季土壤纤毛虫优势种生态位及其与环境因子的关系,于2015年10月在亏祖山共设置11个样点,用土壤采样器采用梅花五点式采集土样,同时记录相应的理化因子,土样在实验室自然风干后,采用“非淹没培养法”和“活体观察法”进行培养和形态学鉴定。亏祖山11个样点中,共鉴定到土壤纤毛虫132种,隶属3纲14目48科66属,其中14个优势种(Y>0.02)。土壤纤毛虫优势种生态位宽度最大的是明显长颈虫(Dileptus conspicuus),生态位宽度最小的是卵圆前管虫(Prorodon ovum)和似肾形虫(Colpoda simulans)。土壤纤毛虫优势种生态位重叠值位于0.02—0.99之间,生态位重叠值最低的为似肾形虫(Colpoda simulans)和卵圆前管虫(Prorodon ovum),而生态位重叠值最高的为念珠角毛虫(Keronopsis monilata)和膨胀肾形虫(Colpoda inflata)。亏祖山14个优势种生态响应速率之和为负值,表明土壤纤毛虫优势种处于衰退阶段。土壤纤毛虫优势种与环境因子的典范对应分析(CCA)结果显示,轴1和轴2累计解释物种变异的37.8%,表明轴1和轴2能较好地反映优势种与环境因子的关系;总氮是显著解释变量,对优势种变异的解释率为17.6%。综上分析得出,明显长颈虫(Dileptus conspicuus)、点滴半眉虫(Hemiophrys pectinata)和前口瞬目虫(Glaucoma frontata)对土壤环境适应能力较强,具有广泛的生态适应幅度;土壤纤毛虫优势种生态位重叠值低,种间竞争比较弱;土壤纤毛虫优势种之间以及优势种和环境之间尚未达到相对的动态平衡,优势种正处衰退阶段;总氮是影响拉萨河流域土壤纤毛虫优势种分布和生态位的主要环境因子。

关键词：亏祖山土壤纤毛虫生态宽度生态位重叠值环境因子

1000MW水轮发电机定子线棒绝缘技术的研究

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绝缘材料 2012年第3期45卷 16-21页

作者：徐冰李振海张秋寒赫兟哈尔滨电机厂有限责任公司哈尔滨150040

介绍了1 000 MW水轮发电机定子线棒绝缘技术及定子线棒的电气性能,通过对定子线棒绝缘结构和防晕结构的优化设计以及对材料的改进,定子线棒的绝缘性能和防电晕效果得到了较大的提高,尤其是提高了定子线棒的电性能,保证了1 000 MW水轮发... 详细信息

介绍了1 000 MW水轮发电机定子线棒绝缘技术及定子线棒的电气性能,通过对定子线棒绝缘结构和防晕结构的优化设计以及对材料的改进,定子线棒的绝缘性能和防电晕效果得到了较大的提高,尤其是提高了定子线棒的电性能,保证了1 000 MW水轮发电机定子绕组的运行寿命。

关键词：水轮发电机定子线棒绝缘结构防晕结构

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城市居民公共安全感的空间格局分布研究

华北科技学院学报 2023年第2期20卷 106-111,124页

作者：张秋涵熊亚超祁慧华北科技学院安全工程学院北京东燕郊065201 中国矿业大学公共管理学院江苏徐州221116 华北科技学院经济管理学院北京东燕郊065201

遵循公共安全感的多维性,从城市治理、社会发展及居民日常生活等角度构建了公共安全感的测量指标体系,基于2021年CCPSD的调研数据资料,观测居民公共安全感的空间格局分布样态。结果表明:公共安全感的权重表明公众的安全需求集中在自然... 详细信息

遵循公共安全感的多维性,从城市治理、社会发展及居民日常生活等角度构建了公共安全感的测量指标体系,基于2021年CCPSD的调研数据资料,观测居民公共安全感的空间格局分布样态。结果表明:公共安全感的权重表明公众的安全需求集中在自然灾害、交通、治安及社会保障等领域;城市居民公共安全感总体偏低,且各地区间差距明显;不同维度的公共安全感亦呈现出复杂的现实格局。基于此,提出以提高居民安全感知绩效为抓手,从“重监管、促公平及精细化”三方面给出公共安全感的提升路径。

关键词：城市居民公共安全感熵权法空间格局

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芳樟醇体外抗红色毛癣菌活性及机理研究

中国真菌学杂志 2022年第6期17卷 441-447页

作者：成福张秋寒唐晓莲王万能重庆理工大学药学与生物工程学院重庆400054 重庆大学附属肿瘤医院中国药房编辑出版中心重庆400042

目的探究芳樟醇抗红色毛癣菌的作用及机理。方法采用改良CLSI M38-A2法测定芳樟醇抗红色毛癣菌的最小抑菌浓度;显微观察芳樟醇对红色毛癣菌的孢子萌发、菌丝形态的影响;电导率法判定芳樟醇对红色毛癣菌细胞膜的作用,并利用紫外分光光... 详细信息

目的探究芳樟醇抗红色毛癣菌的作用及机理。方法采用改良CLSI M38-A2法测定芳樟醇抗红色毛癣菌的最小抑菌浓度;显微观察芳樟醇对红色毛癣菌的孢子萌发、菌丝形态的影响;电导率法判定芳樟醇对红色毛癣菌细胞膜的作用,并利用紫外分光光度分析芳樟醇对红色毛癣菌麦角甾醇合成的影响。透射电镜观察芳樟醇作用红色毛癣菌后其微观结构的改变。通过构建红色毛癣菌感染豚鼠模型,以评价芳樟醇对皮肤癣病的治疗效果。结果芳樟醇抗红色毛癣菌的最小抑菌浓度为320μg·mL^(-1);芳樟醇可抑制红色毛癣菌的孢子萌发及菌丝的径向生长并导致细胞膜通透性增加以及抑制细胞膜中麦角甾醇的合成。显微观察结果显示细胞壁被降解,表面模糊、细胞内膜皱缩、细胞内容物泄漏。动物实验表明,芳樟醇可明显控制皮肤癣病的进程,改善豚鼠组织损伤程度。结论芳樟醇具有抑制红色毛癣菌的活性,通过抑制其孢子萌发、菌丝生长,以及降解细胞壁、破坏细胞膜的完整性和抑制麦角甾醇合成等方式抑制红色毛癣菌的生长。

关键词：芳樟醇红色毛癣菌抗菌机理麦角甾醇

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马钢冷轧变频调速异步电动机定子线圈制造工艺技术研究

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防爆电机 2005年第2期40卷 33-34,48页

作者：郭庆华梁锐张秋寒哈尔滨电机厂有限责任公司黑龙江哈尔滨150040

马钢冷轧电机定子主绝缘采用少胶VPI体系,定子匝间绝缘、股间绝缘特殊,并首次采用防浸渍漆流失的国产单面透气保护带。

关键词：定子线圈异步电动机主绝缘匝间绝缘浸渍漆变频调速 VPI 冷轧马钢制造工艺技术