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Effect of Deep Sea Pressures on the Corrosion Behavior of X65 Steel in the Artificial Seawater

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Acta Metallurgica Sinica(English Letters) 2019年第8期32卷 972-980页

作者： qiu-shi li Shun-Zhong Luo Xu-Teng Xing Jing Yuan Xin liu Ji-Hui Wang Wen-Bin Hu Tianjin Key Laboratory of Composite and Functional Materials School of Materials Science and EngineeringTianjin UniversityTianjin 300072China College of Physics Electronic Information Engineering Qinghai University for NationalitiesXining 810007China

The corrosion behaviors of X65 steel in the artificial seawater at different hydrostatic pressures are investigated by potentiodynamic polarization measurements, electrochemical impedance spectroscopy measurements and weight loss *** corroded morphologies and the corrosion products are also investigated by scanning electron microscopy, X-ray diffraction analysis and Raman *** results show that the corrosion current increases as the hydrostatic pressure *** charge transfer resistance decreases as the hydrostatic pressure *** corrosion products are mainly composed of γ-FeOOH and Fe3O4 at the atmospheric pressure, while the main components are γ-FeOOH, Fe3O4, and γ-Fe2O3 at the high *** hydrostatic pressure accelerates the corrosion of X65 steel due to its effect on the chemical and physical properties of corrosion products, including the promoted reduction of γ-FeOOH and the wider and deeper cracks on the corrosion products layer.

关键词： X65 steel Deep sea pressures Electrochemical measurements Corrosion

Cell viability and extracellular matrix synthesis in a co-culture system of corneal stromal cells and adipose-derived mesenchymal stem cells

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 670-678页

作者： Ting Shen Jiang Shen Qing-Qing Zheng qiu-shi li Hai-Lan Zhao Lei Cui Chao-Yang Hong Zhejiang Provincial People's Hospital Hangzhou 310014Zhejiang Province China Beijing shijitan Hospital Beijing 100050 China Wenzhou Medical University Wenzhou 325035 Zhejiang Province China

AIM：To investigate the impact of adipose-derived mesenchymal stem cells（ADSCs） on cell viability and extracellular matrix（ECM） synthesis of corneal stromal cells（CSCs）. METHODS：ADSCs and CSCs were obtained from the corneas of New Zealand white rabbits and indirectly cocultured in vitro. The proliferative capacity of CSCs in the different groups was assessed by CCK-8 assays. Annexin V-fluorescein isothiocyanate（FITC）/proliferation indices（PI） assays were used to detect the apoptosis of CSCs. The expression levels of matrix metalloproteinase（MMP）, such as MMP1, MMP2, MMP9, and collagens were also evaluated by Western blot. RESULTS：ADSCs significantly promoted proliferation and invasion of CSCs in the indirect co-culture assays. The co-cultural group displayed much higher ability of proliferation, especially under the co-culture conditions of ADSCs for 3d, compared with that CSCs cultured alone. The PI of CSCs in the co-culture system were increased approximately 3-8-fold compared with the control group. A significant change was observed in the proportions of cells at apoptosis（early and late） between the negative control group（6.34% and 2.06%） and the ADCSs-treated group（4.69% and 1.59%）. The expression levels of MMPs were down regulated in the co-culture models. Compared with the control group, the decrease intensities of MMP-1, MMP-2 and MMP-9 in CSCs/ADSCs group were observed, 3.90-fold, 1.09-fold and 3.03-fold, respectively. However, the increase intensities of collagen type（I, II, III, IV, and V） in CSCs were observed in CSCs/ADSCs group, 3.47-fold,4.30-fold, 2.35-fold, 2.55-fold and 2.43-fold, respectively, compared to that in the control group. The expressions of aldehyde dehydrogenase and fibronectin in CSCs were upregulated in the co-culture ***：ADSCs play a promotive role in CSCs＇ growth and invasion, which may be partially associated with MMPs decrease and collagens increase, resulting in a positive participation in the plasticity and ECM synt

关键词： adipose-derived mesenchymal stem cell corneal stromal cells extracellular matrix plasticity

Polarization behaviors in a transverse Ising film with multi-surface layers

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Polarization behaviors in a transverse Ising film with multi...

Effects of Adipose-derived Mesenchymal Stem Cell Exosomes on Corneal Stromal Fibroblast Viability and Extracellular Matrix Synthesis

2015 2nd International Conference on Machinery,Materials Engineering,Chemical Engineering and Biotechnology(MMECEB 2015)

作者： qiu shi li College of Mechanical Engineering Linyi University

The mean-field theory is used to study polarization behaviors in a ferroelectric thin film described by the transverse Ising *** dependence of average polarization and layer polarizations of the transverse Ising film with two surface layers on the Curie temperature or transverse field is discussed in *** numerical calculations are also compared with those of the effective-field theory with correlations.

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Chinese Medical Journal 2018年第6期131卷 704-712页

作者： Ting Shen Qing-Qing Zheng Jiang Shen qiu-shi li Xing-Hui Song Hong-Bo Luo Chao-Yang Hong Ke Yao Eye Institute of Zhejiang University Eye Center of Second Affiliated Hospital of Zhejiang University School of Medicine Hangzhou Zhejiang 310009 China Department of Ophthalmology Zhejiang Provincial People's Hospital and People's Hospital of Hangzhou Medical College Hangzhou Zhejiang 310014 China School of Ophthalmology and Optometry Wenzhou Medical University Wenzhou Zhejiang 325035 China Facility for Biochemistry and Molecular Medicine Core Facilities School of Medicine Zhejiang University Hangzhou Zhejiang 310058 China

Background： Corneal stromal cells （CSCs） are components of the corneal endothelial microenvironment that can be induced to form a functional tissue-engineered corneal endothelium. Adipose-derived mesenchymal stem cells （ADSCs） have been reported as an important component of regenerative medicine and cell therapy for corneal stromal damage. We have demonstrated that the treatment with ADSCs leads to phenotypic changes in CSCs in vitro. However, the underlying mechanisms of such ADSC-induced changes in CSCs remain unclear. Methods： ADSCs and CSCs were isolated from New Zealand white rabbits and cultured in vitro. An Exosome Isolation Kit, Western blotting, and nanoparticle tracking analysis （NTA） were used to isolate and confirm the exosomes from ADSC culture medium. Meanwhile, the optimal exosome concentration and treatment time were selected. Cell Counting Kit-8 and annexin V-fluorescein isothiocyanate/ propidium iodide assays were used to assess the effect of ADSC-derived exosomes on the proliferation and apoptosis of CSCs. To evaluate the effects ofADSC-derived exosomes on CSC invasion activity, Western blotting was used to detect the expression of matrix metalloproteinases （MMPs） and collagens. Results： ADSCs and CSCs were successfully isolated from New Zealand rabbits. The optimal concentration and treatment time of exosomes for the following study were 100 μg/ml and 96 h, respectively. NTA revealed that the ADSC-derived exosomes appeared as nanoparticles （40-200 nm）, and Western blotting confirmed positive expression of CD9, CDSI, flotillin-1, and HSP70 versus ADSC cytoplasmic proteins （all P〈 0.01 ）. ADSC-derived exosomes （50μg/ml and 100μg/ml） significantly promoted proliferation and inhibited apoptosis （mainly early apoptosis） of CSCs versus non-exosome-treated CSCs （all P 〈 0.05）. Interestingly, MMPs were downregulated and extracellular matrix （ECM）-related proteins including collagens and fibronectin were upregulated in the exosome-treated CSCs versus non-

关键词： Adipose-derived Mesenchymal Stem Cell Corneal Stromal Cells Exosomes Extracellular Matrix Synthesis

Prophylactic effects of sialylated glycopeptides from edible bird’s nest on neuroinflammation in lipopolysaccharide-treated mice via the gut-brain axis

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Food Science and Human Wellness 2024年

作者： Xiang-Rong Cheng Chen-Xi Zhang Xiao-Yi Zhao qiu-shi li Yu Song Lan Zhang Jia-Hui Ma Yu-Yao Chen Man Yuan Dong-liang Wang School of Food Science and Technology， Jiangnan University State Key Laboratory of Food Science and Resources， Jiangnan University Central Nervous System Drug Key Laboratory of Sichuan Province Luzhou Hebei Edible Bird's Nest Fresh Stew Technology Innovation Center

Edible bird’s nest (EBN)， abundant in sialic acid (SA) has been recognized as a natural substance beneficial to brain development and cognitive ability. In order to investigate active component of EBN， sialylated glycopeptides (SCP) were extracted from EBN by 60% ethanol after trypsin hydrolysis and its effects on central nervous system was evaluated in lipopolysaccharide (LPS)-induced neuroinflammation mice. EBN， SCP， and SA pretreatment and intervention reduced IL-1β， TNF-α， and IL-6 levels in hippocampus and cortex， meanwhile inhibited the activation of microglia， astrogliosis and neuron apoptosis， which were consistent with the learning and memory improvement and anti-depression effects in behavioral tests. The counts of leukocytes， neutrophils and monocytes， as well as IL-1β， TNF-α， and IL-6 levels in peripheral circulation were significantly reduced by SCP. Results of plasma metabolomics suggested SCP up-regulated energy metabolism， promoted the recovery of primary and secondary bile acid metabolism and indole metabolism， where microbiota may involve. 16S rDNA sequencing of colonic contents showed EBN， SCP and SA repaired dysbacteriosis in LPS-treated mice by significantly up-regulating the anti-inflammatory Muribaculaceae and inhibiting pro-inflammatory related Desulfovibrio and Candidatus＿Saccharimonas. In addition， both EBN and SCP could significantly enrich ***， while SA could specifically enrich Prevotellaceae＿UCG＿001. The gut-brain axis was preliminarily established， and SCP may have the potential to be a functional factor for neuroprotection applied in EBN industry.

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An investigation on the wind profiles and gravity wave dynamics in MLT region based on the meteor radars from the Meridian Project

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Earth and Planetary Physics 2025年第1期9卷 29-38页

作者： Tai liu Zhe Wang MengXi shi Willie Soon shiCan qiu Department of Geophysics College of the Geology Engineering and GeomaticsChang’an UniversityXi’an 710054China Center for Environmental Research and Earth Sciences(CERES) SalemMA 01970USA Institute of Earth Physics and Space Science(ELKH EPSS) 9400SopronHungary

The meteor radar can detect the zenith angle,azimuth,radial velocity,and altitude of meteor trails so that one can invert the wind profiles in the mesosphere and low thermosphere(MLT)region,based on the Interferometric and Doppler *** this paper,the horizontal wind field,gravity wave(GW)disturbance variance,and GW fluxes are analyzed through the meteor radar observation from 2012−2022,at Mohe(53.5°N,122.4°E)and Zuoling(30.5°N,114.6°E)stations of the(Chinese)Meridian *** Lomb−Scargle periodogram method has been utilized to analyze the periodic variations for time series with observational data *** results show that the zonal winds at both stations are eastward dominated,while the meridional winds are southward *** variance of GW disturbances in the zonal and meridional directions increases gradually with height,and there is a strong pattern of annual *** zonal momentum flux of GW changes little with height,showing weak annual *** meridional GW flux varies gradually from northward to southward with height,and the annual periodicity is *** both stations,the maximum values of zonal and meridional wind occur close to the peak heights of GW flux,with opposite *** observational evidence is consistent with the filtering *** horizontal wind velocity,GW flux,and disturbance variance of the GW at Mohe are overall smaller than those at Zuoling,indicating weaker activities in the MLT at *** power spectral density(PSD)calculated by the Lomb−Scargle periodogram shows that there are 12-month period and 6-month period in horizontal wind field,GW disturbance variance and GW flux at both stations,and especially there is also a 4-month cycle in the disturbance *** PSD of the 12-month and 6-month cycles exhibits maximum values below 88 km and above 94 km.

关键词： meteor radar wind field gravity wave Lomb−Scargle method

维拉帕米对体外人晶状体上皮细胞增殖和黏附的抑制作用

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国际眼科杂志 2011年第1期11卷 28-30页

作者：李秋实刘丹辽宁医学院附属第一医院眼科中国辽宁省锦州市121001

目的：研究钙离子拮抗剂维拉帕米（verapamil,Ver）对体外培养的人晶状体上皮细胞（human lens epithelial cell,HLEC）增殖、凋亡和黏附的影响。方法：组织块法培养人晶状体上皮细胞，用Ver处理第二代人晶状体上皮细胞。MTT法观察不同... 详细信息

目的：研究钙离子拮抗剂维拉帕米（verapamil,Ver）对体外培养的人晶状体上皮细胞（human lens epithelial cell,HLEC）增殖、凋亡和黏附的影响。方法：组织块法培养人晶状体上皮细胞，用Ver处理第二代人晶状体上皮细胞。MTT法观察不同浓度的Ver对HLEC增殖和黏附的影响，AO/EB双染法观察细胞形态，Annexin V-FITC/PI双染法观察细胞凋亡情况，流式细胞术（FCM）检测Ver对HLEC周期的影响。结果：组织块贴壁4~6d可见HLEC从组织块边缘长出，15~20d接近融合。随着Ver药物浓度的增加，HELC增殖率减少，以72h最为明显。FCM检测细胞周期变化时发现，G1期细胞在10,40g/L Ver（78.1±0.2，83.2±0.6）较正常组（72.0±0.9）增加（P〈0.05）；S期的细胞在10,40g/L Ver（10.2±0.1,8.5±0.3）较正常组（16.9±0.3）明显减少（P〈005）。形态学观察发现，HELC在40g/L Ver作用48h后发生了一系列形态改变，包括细胞核固缩、染色质边集等凋亡改变。10，20，40，80g/L的Ver可以诱导HLEC发生凋亡，且随着浓度的升高凋亡率显著升高。用药24h后HELC的贴壁量为对照组的73.2%。结论：Ver能改变细胞周期而抑制HLEC增殖、诱导细胞凋亡，并且抑制HLEC的黏附。

关键词：人晶状体上皮细胞增殖黏附凋亡细胞周期

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锦屏深部地下实验室初始地应力测量实践

岩土力学 2018年第1期39卷 356-366页

作者：钟山江权冯夏庭刘继光李邵军邱士利吴世勇中国科学院武汉岩土力学研究所岩土力学与国家重点实验室湖北武汉430071 中国科学院大学北京100049 雅砻江流域水电开发有限公司四川成都610051

地应力条件是大型地下洞室稳定性分析与工程设计的必要信息之一,对于深部高应力地下工程的安全评价与灾害防治尤为重要。以埋深2 400 m的中国锦屏地下实验室为例,首先重点阐述了高应力下条件下应力解除法测量地应力的原理与方法,并提出... 详细信息

地应力条件是大型地下洞室稳定性分析与工程设计的必要信息之一,对于深部高应力地下工程的安全评价与灾害防治尤为重要。以埋深2 400 m的中国锦屏地下实验室为例,首先重点阐述了高应力下条件下应力解除法测量地应力的原理与方法,并提出了针对在高应力条件下使用36-2型钻孔变形计地应力测量的改进技术:(1)采用新式阶梯状钻头,分级逐步解除,减小岩芯根部应力集中区域的范围和应力大小;(2)使用大直径解除钻头来增加套孔空心圆柱状岩芯的厚度,即增大裂隙贯通整个岩芯的时间;(3)在测点附近钻取勘探孔以获知测点岩体完整性条件,对是否适合开展测试进行评估。在此基础上,通过现场岩体地应力测试获得每个测孔不同深度垂直钻孔轴线平面上的平面应力状态以及三孔交汇处的三维应力状态(3个主应力的大小和方向),分析开挖扰动导致的围岩应力重分布规律与原始应力场分布。依据洞室围岩重分布应力特征与现场实际破坏的空间位置对应关系,结合现场破坏情况和工程区域地质条件,多角度验证了三维应力结果是可靠的,可为进一步的工程稳定性评价以及围岩支护设计提供依据。

关键词：地应力测量套孔应力解除法地下实验室高应力岩芯饼化

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深部硬岩剪切边界下应力诱发片帮的机制研究

岩土力学 2022年第4期43卷 1103-1111,1122页

作者：高要辉张春生苏方声邱士利中国电建集团华东勘测设计研究院有限公司浙江杭州310014 杭州市拱墅区拱宸桥街道办事处浙江杭州310015 中国科学院武汉岩土力学研究所岩土力学与工程国家重点实验室湖北武汉430071

深部工程开挖现场发现硬岩的剪切破坏过程对地下工程稳定性具有重要影响。深埋硬岩隧洞边墙处片帮或板裂主要为压致拉裂的结果,可通过室内单轴压缩试验来模拟;而拱肩和底角处的片帮或板裂多数是在剪切边界下形成的,需通过室内完整硬岩... 详细信息

深部工程开挖现场发现硬岩的剪切破坏过程对地下工程稳定性具有重要影响。深埋硬岩隧洞边墙处片帮或板裂主要为压致拉裂的结果,可通过室内单轴压缩试验来模拟;而拱肩和底角处的片帮或板裂多数是在剪切边界下形成的,需通过室内完整硬岩直剪试验来再现。针对不同类型硬岩压致拉裂型片帮,已进行了相当深入的研究,但对完整硬岩剪切边界下应力诱发型片帮的直接研究还存在着不足。为了深入研究深埋硬岩隧洞片帮或板裂的形成机制,采用FLAC;软件模拟隧洞不同位置处围岩受开挖扰动的应力调整过程,根据中国锦屏地下实验室二期工程区域的片帮统计结果,将深埋硬岩隧洞片帮划分为压致拉裂型和剪切边界应力诱发型两类,提出剪切边界应力诱发型片帮形成过程的概念模型;利用完整大理岩设计了不同正应力下压剪破裂到剪切滑移的连续性直剪试验,试样典型片帮破坏结果验证了概念模型的合理性。

关键词：剪切破坏完整大理岩片帮或板裂概念模型直剪试验