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Switching On/Off of Guide RNA by Photoinduced Strand Displacement for Functional Control of CRISPR/Cas9

CCS Chemistry 2024年第5期6卷 1338-1351页

作者： Yang Wang Chao-Qun Wu qiu-Long Zhang Ming Shao Yan Liu Liang-Liang Wang Zun-Yun Wang juan Du Liang Xu Central Laboratory Department of the Second Affiliated Hospital School of MedicineThe Chinese University of Hong KongShenzhen&Longgang District People’s Hospital of ShenzhenShenzhen 518172 Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical EngineeringShenzhen University Medical SchoolShenzhen 518060 MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510275 School of Pharmacy and Medical Technology Putian UniversityPutian 351100

Functional control of CRISPR/Cas9 is essential for precise gene *** engineering of guide RNA(gRNA)provides diverse approaches for conditional control of CRISPR/Cas9 function with a variety of chemical reactive ***,previous investigations into chemically engineering gRNA only unidirectionally regulated the CRISPR/Cas9 function via stimuli-induced caging/decaging ***,we propose a combinatory strategy to engineer the dynamics of gRNA in which photocontrolled strand-displacement reactions coupled with sequence designs of gRNA can achieve lightinduced switching-on/off control of CRISPR/Cas9 *** analysis and cellular gene regulation indicate this approach is capable of both activating and deactivating CRISPR/Cas9 activities using light ***,photocontrolled multiplex modulations of gene expression for opposite regulatory effects have also been achieved simultaneously under the same cellular *** work establishes an essential principle for construction of stimuli-induced switching-on/off modulations of gRNA that can greatly enrich the versatility of conditional control for a variety of CRISPR/Cas9-based applications.

关键词： modified oligonucleotides gene manipulation optical control CRISPR/Cas9 strand displacement

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Textured Asymmetric Membrane Electrode Assemblies of Piezoelectric Phosphorene and Ti_(3)C_(2)T_(x)MXene Heterostructures for Enhanced Electrochemical Stability and Kinetics in LIBs

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Nano-Micro Letters 2024年第4期16卷 394-414页

作者： Yihui Li juan Xie Ruofei Wang Shugang Min Zewen Xu Yangjian Ding Pengcheng Su Xingmin Zhang Liyu Wei Jing‑Feng Li Zhaoqiang Chu Jingyu sun Cheng Huang Volta and DiPole Materials Labs College of EnergySoochow Institute for Energy and Materials InnovationS(SIEMIS)Key Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu ProvinceKey Laboratory of Core Technology of High Specific Energy Battery and Key Materials for Petroleum and Chemical IndustrySoochow UniversitySuzhou 215006People’s Republic of China School of Materials Science and Engineering Jiangsu UniversityZhenjiang 212013People’s Republic of China College of Underwater Acoustic Engineering Harbin Engineering UniversityHarbin 150001People’s Republic of China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteShanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201204People’s Republic of China State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing 100084People’s Republic of China High Density Materials Technology Center for Flexible Hybrid Electronics Suzhou Institute of Electronic Functional Materials TechnologySuzhou Industrial Technology Research InstituteSuzhou 215151People’s Republic of China Institute of Advanced Materials and Institute of Membrane Science and Technology Jiangsu National Synergistic Innovation Center for Advanced MaterialsSuzhou Laboratory and Nanjing Tech UniversityNanjing 211816People’s Republic of China

Black phosphorus with a superior theoretical capacity(2596 mAh g^(-1))and high conductivity is regarded as one of the powerful candidates for lithium-ion battery(LIB)anode materials,whereas the severe volume expansion and sluggish kinetics still impede its applications in *** contrast,the exfoliated two-dimensional phosphorene owns negligible volume variation,and its intrinsic piezoelectricity is considered to be beneficial to the Li-ion transfer kinetics,while its positive influence has not been discussed ***,a phosphorene/MXene heterostructure-textured nanopiezocomposite is proposed with even phosphorene distribution and enhanced piezo-electrochemical coupling as an applicable free-standing asymmetric membrane electrode beyond the skin effect for enhanced Li-ion *** experimental and simulation analysis reveals that the embedded phosphorene nanosheets not only provide abundant active sites for Li-ions,but also endow the nanocomposite with favorable piezoelectricity,thus promoting the Li-ion transfer kinetics by generating the piezoelectric field serving as an extra *** waltzing with the MXene framework,the optimized electrode exhibits enhanced kinetics and stability,achieving stable cycling performances for 1,000 cycles at 2 A g^(-1),and delivering a high reversible capacity of 524 m Ah g^(-1)at-20℃,indicating the positive influence of the structural merits of self-assembled nanopiezocomposites on promoting stability and kinetics.

关键词： Phosphorene Nanopiezocomposite Piezo-electrochemical coupling Membrane electrode assembly Lithium-ion storage

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Caliper-based precise positioning of the target(CALIPPOT)for transcranial magnetic stimulation without neuronavigation system

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General Psychiatry 2024年第2期37卷 311-314页

作者： Yunsong Hu Rong Zeng juan Yue qiu Ge Hongxiao Wang Zijian Feng Jue Wang Yufeng Zang Center for Cognition and Brain Disorders The Affiliated Hospital of Hangzhou Normal UniversityHangzhouZhejiangChina Zhejiang Key Laboratory for Research in Assessment of Cognitive Impairments HangzhouZhejiangChina Institutes of Psychological Sciences Hangzhou Normal UniversityHangzhouZhejiangChina Transcranial Magnetic Stimulation Center Deqing Hospital of Hangzhou Normal UniversityDeqingZhejiangChina Institute of Sports Medicine and Health Chengdu Sport UniversityChengduSichuanChina Department of Neurology The Affiliated Hospital of Hangzhou Normal UniversityHangzhouZhejiangChina

To the editor:Transcranial magnetic stimulation(TMS)is a non-invasive brain modulation *** important usage of TMS is the transient interruption of cognitive brain function(also named virtual lesion)for investigating precisely where and when a specific cortical region contributes to a specific cognitive function.1 A more important usage of TMS is the treatment of brain disorders by repetitive TMS(rTMS).

关键词： stimulation technique. precise

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Meiotic transcriptional reprogramming mediated by cell-cell communications in humans and mice revealed by scATACseq and scRNA-seq

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Zoological Research 2024年第3期45卷 601-616页

作者： Hai-Quan Wang Xiao-Long Wu Jing Zhang Si-Ting Wang Yong-juan Sang Kang Li Chao-Fan Yang Fei sun Chao-Jun Li State Key Laboratory of Reproductive Medicine and Offspring Health Center for Global HealthSchool of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Medical School Nanjing UniversityNanjingJiangsu 210093China Zhejiang University School of Medicine HangzhouZhejiang 310016China Department of Urology and Andrology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhouZhejiang 310000China Model Animal Research Center of Medical School Nanjing UniversityNanjingNanjingJiangsu 210093China Liangzhu Laboratory Zhejiang UniversityHangzhouZhejiang 311121China

Meiosis is a highly complex process significantly influenced by transcriptional ***,studies on the mechanisms that govern transcriptomic changes during meiosis,especially in prophase I,are ***,we performed single-cell ATAC-seq of human testis tissues and observed reprogramming during the transition from zygotene to pachytene *** event,conserved in mice,involved the deactivation of genes associated with meiosis after reprogramming and the activation of those related to spermatogenesis before their functional ***,we identified 282 transcriptional regulators(TRs)that underwent activation or deactivation subsequent to this *** suggested that physical contact signals from Sertoli cells may regulate these TRs in spermatocytes,while secreted ENHO signals may alter metabolic patterns in these *** results further indicated that defective transcriptional reprogramming may be associated with non-obstructive azoospermia(NOA).This study revealed the importance of both physical contact and secreted signals between Sertoli cells and germ cells in meiotic progression.

关键词： Single-cell RNA-seq Single-cell ATAC-seq Spermatogenesis Meiosis Transcriptional reprogramming Cell-cell communication

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Reinforcing oxygen electrocatalytic activity via selective dualphase heterointerface engineering for rechargeable Zn-air batteries

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Rare Metals 2024年第4期43卷 1524-1536页

作者： Chao Cheng Chuang Zhi Zhong-Ti sun Yong-Qiang Ming Ting-Ting Xiang Qing-Chao Zhu Zi-Rui Wu Bing Li Yi Li Cheng Jin Yong Cao juan Yang School of Materials Science and Engineering Jiangsu UniversityZhenjiang212013China Faculty of Material Science and Engineering Kunming University of Science and TechnologyKunming650093China

Dual-phase heterointerface electrocatalysts(DPHE)constructed by oxygen reduction reaction(ORR)-and oxygen evolution reaction(OER)-active elements exhibit excellent bifunctional activity and long-term durability due to the abundant interface exposure and synergistic catalytic ***,low-dimensional N-doped graphene nanoribbons(N-GNRs)coupling with ultrathin CoO nanocomposites(N-GNRs/CoO)were controllably fabricated through a facile two-step approach using synthesized Co(OH)_2 nanosheet as CoO *** functional theory(DFT)calculations and experimental characterizations prove that the formation of interface between N-GNRs and CoO can induce local charge redistribution,contributing to the improvement of catalytic activity and *** optimal N-GNRs/CoO DPHE possesses hierarchically porous architectures and presents outstanding bifunctional activities with a small potential gap of 0.729 V between the potential at 10 mA·cm^(-2)for OER and the halfwave potential for ORR,which outperforms Pt/C+IrO_(2)and the majority of noble-metal-free bifunctional ***-and solid-state rechargeable Zn-air batteries assembled with N-GNRs/CoO as the cathode also display high peak power density and fantastic cycle stability,superior to that of benchmark Pt/C+IrO_(2)*** is anticipated to offer significant benefits toward high activity,stability and mechanical flexibility bifunctional oxygen electrocatalysts for rechargeable Zn-air batteries.

关键词： Interface engineering Graphene nanoribbons CoO Bifunctional electrocatalyst Zn-air batteries

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Experimental Study on Multistage Seismic Damage Process of Bedding Rock Slope: A Case Study of the Xinmo Landslide

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Journal of Earth Science 2024年第5期35卷 1594-1612页

作者： Jing-Jing Tian Tian-Tao Li Xiang-Jun Pei Jian Guo Shou-Dao Wang Hao sun Pei-Zhang Yang Run-qiu Huang State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology Department of Civil Engineering Panzhihua University Power China Chengdu Engineering Corporation Limited

In the early hours of June 24, 2017, a major landslide event occurred in Xinmo Village, Sichuan Province, China. The landslide instantly devastated the whole village. Ten people died and 73 were missing in this major landslide event. The study area has suffered from several strong earthquakes in the past 100 y. Present studies have reported that the cumulative damage effect of the Xinmo landslide induced by earthquake is obvious. In this study, we conducted a shaking table test based on the detailed geological survey, historical seismic data, satellite optical image, unmanned aerial vehicle photography. The test result presents the characteristics of multistage seismic damage and progressive deformation process of the Xinmo landslide model, and shows that the historical earthquakes have caused serious damage to the interior of rock mass in the source area. The test also shows that the cumulative damage of the model increases with an increase in duration of earthquake loading. When the excitation intensity increases to a certain value, the damage accumulation velocity of the model suddenly increases. It reveals that frequent historical earthquake loads can be regarded as a main reason for the damage and deterioration of landslide rock mass. Damage accumulation and superposition occur in the slope. Under a long-term gravity, deformation of the slope gradually increases until catastrophic failure is triggered. The progressive deformation process of slope is summarized. Firstly, under strong earthquakes loading, a tensile fracture surface forms at the rear edge of the wavy deformation high and steep bedding slope. It reaches a certain critical depth and expands along the interlayer structural plane. Meantime, damaged fissures perpendicular to the structural plane also appear in the steep-gentle turning area of the slope. Secondly, under a coupling action of seismic loading and gravity, the interlaminar tensile crack surface at the rear edge of the slope extends to depth

关键词： Xinmo landslide damage evolution mechanism progressive deformation process engi-neeringgeolgoy

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Photodoping-Modified Charge Density Wave Phase Transition in WS_(2)/1T-TaS_(2) Heterostructure

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Chinese Physics Letters 2024年第5期41卷 144-170页

作者：王瑞丁建伟孙飞赵继民裘晓辉 Beijing Information Technology College Beijing 100015China CAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Max Planck Institute for Chemical Physics of Solids Dresden 01187Germany Songshan Lake Materials Laboratory Dongguan 523808China

Controlling collective electronic states hold great promise for development of innovative devices. Here, we experimentally detect the modification of the charge density wave(CDW) phase transition within a 1T-TaS_(2) layer in a WS_(2)/1T-TaS_(2) heterostructure using time-resolved ultrafast spectroscopy. Laser-induced charge transfer doping strongly suppresses the commensurate CDW phase, which results in a significant decrease in both the phase transition temperature(T_(c)) and phase transition stiffness. We interpret the phenomenon that photoinduced hole doping, when surpassing a critical threshold value of ~ 10^(18)cm^(-3), sharply decreases the phase transition energy barrier. Our results provide new insights into controlling the CDW phase transition, paving the way for optical-controlled novel devices based on CDW materials.

关键词： doping transition Transition

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Iodine enrichment in the groundwater in South China and its hydrogeochemical control

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Journal of Environmental Sciences 2024年第8期142卷 226-235页

作者： Fengping Zhou Qianting Xu Yuyun Chen Weihua Zhang Rongliang qiu Guangdong Provincial Key Laboratory of Environmental Pollution and Remediation Technology Guangdong Provincial Engineering Research Center for Heavy Metal Contaminated Soil RemediationSchool of Environmental Science and EngineeringSun Yat-sen UniversityGuangzhou 510275China Shenzhen Research Institute Sun Yat-sen UniversityShenzhen 518057China Guangdong Provincial Key Laboratory of Agricultural&Rural Pollution Abatement and Environmental Safety School of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China

In North China,iodine-rich groundwater has been extensively studied,but few in South *** study aimed to investigate the characteristics of iodine-rich groundwater in South China and identify potential contamination *** results revealed that the average concentration of iodine in groundwater was 890μg/L,with a maximumconcentration of 6350μg/L,exceeding the permitted levels recommended by the World Health Organization(5–300μg/L).Notably,the enrichment of iodide occurred in acidic conditions(pH=6.6)and a relatively low Eh environment(Eh=198.4 mV).Pearson correlation and cluster analyses suggested that the enrichment of iodide could be attributed to the intensified redox process involving Mn(II),iodine(I_(2)),or iodate(IO_(3)^(−))in the *** strong affinity between Mn(II)and I_(2)/IO_(3)^(−)facilitated their interaction,resulting in the formation and mobilization of I^(−)from the soil to the *** experiments further confirmed that reducing substances(such as sodium sulfides,ascorbic acids,and fulvic acids)in the soil with low dissolved oxygen(DO)levels(3.8 mg/L)promoted the oxidation of I^(−)into I_(2)or IO_(3)^(−),leading to its *** research provides new insights into the characteristics and mechanisms of I^(−)enrichment in groundwater in South China,and emphasizes the significance of the redox reactions involving Mn(II)and I_(2)/IO_(3)^(−),as well as the influence of soil properties in regulating the occurrence and transportation of iodine species within groundwater systems.

关键词： Iodine enrichment Acid environment Groundwater South China Manganese

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Development of LAG-3/FGL1 blocking peptide and combination with radiotherapy for cancer immunotherapy

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1150-1165页

作者： Yuzhen Qian Yixuan sun Peishang Shi Xiuman Zhou Qiongqiong Zhang Qingyu Dong Shengzhe Jin Lu qiu Xiaoshuang Niu Xiaowen Zhou Wenshan Zhao Yahong Wu Wenjie Zhai Yanfeng Gao School of Life Sciences Zhengzhou UniversityZhengzhou 450001China School of Pharmaceutical Sciences(Shenzhen) Shenzhen Campus of Sun Yat-sen UniversityShenzhen 518107China International Joint Laboratory for Protein and Peptide Drugs of Henan Province Zhengzhou UniversityZhengzhou 450001China

Aside from antibodies,peptides show great potential as immune checkpoint inhibitors(ICIs)due to several advantages,such as better tumor penetration and lower ***-activation gene 3(LAG-3)is an immune checkpoint which can induce T cell dysfunction through interaction with its soluble ligand fibrinogen like protein-1(FGL1).Here,we found that LAG-3 expression was higher than programmed cell death protein 1(PD-1)in multiple human cancers by TCGA databases,and successfully identified a LAG-3 binding peptide LFP-6 by phage display bio-panning,which specifically blocks the interaction of LAG-3/FGL1 but not LAG-3/***,D-amino acids were introduced to substitute the N-and C-terminus of LFP-6 to obtain the proteolysis-resistant peptide LFP-D1,which restores T cell function in vitro and inhibits tumor growth in ***,a bispecific peptide LFOP targeting both PD-1/PD-L1 and LAG-3/FGL1 was designed by conjugating LFP-D1 with PD-1/PD-L1blocking peptide OPBP-1(8-12),which activates T cell with enhanced proliferation and IFN-γ *** importantly,LFOP combined with radiotherapy significantly improve the T cell infiltration in tumor and elevate systemic antitumor immune *** conclusion,we developed a novel peptide blocking LAG-3/FGL1 which can restore T cell function,and the bispecific peptide synergizes with radiotherapy to further enhance the antitumor immune response.

关键词： LAG-3 FGL1 PD-1 PD-L1 Peptide Immune checkpoint Radiotherapy Cancer immunotherapy

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Clinical efficacy and safety of flexible ureteroscopy and percutaneous nephrolithotomy for large kidney stones:A retrospective comparative study

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World Journal of Clinical Cases 2024年第21期12卷 4483-4490页

作者： qiu-Lian Wang Jun-Qiang Liu juan Cao Jun Ding Department of Urology No.904 Hospital of Joint Logistic Support Force of PLAWuxi 214044Jiangsu ProvinceChina

BACKGROUND Renal stones ranging 20–40 mm are very common in *** no largesample clinical studies have confirmed the clinical efficacy and safety of this method,there is also a lack of comparative data with traditional *** To investigate the clinical efficacy of flexible ureteroscopy(FURS)and percutaneous nephrolithotomy(PCNL)by postoperative stone clearance and changes in soluble vascular cell adhesion molecule 1(sVCAM-1)and kidney injury molecule 1(KIM-1)levels in patients with large kidney stones(>2 cm in diameter).METHODS This single-center observational study was performed at a Chinese hospital between January 1,2021,and October 30,*** 250 enrolled patients were diagnosed with large kidney stones(>2 cm)and divided into a FURS group(n=145)and a PCNL group(n=105)by the surgical *** FURS group was treated with flexible ureteroscopy and the PCNL group was treated with percutaneous *** operation time,time to palinesthesia,intraoperative blood loss,drop in hemoglobin,length of hospital stay,stone clearance rate,and complications were recorded in the two *** and postoperative serum sVCAM-1 levels,erythrocyte sedimentation rate(ESR),urine KIM-1 levels,preoperative and postoperative pain visual analog scale(VAS)and Wisconsin Stone Quality of Life Questionnaire(WISQOL)scores were also *** All 250 eligible patients completed the *** were no significant differences in baseline characteristics between the two groups(P>0.05).The operation time in the FURS group was significantly greater than that in the PCNL *** time to ambulation,intraoperative blood loss,decrease in hemoglobin,and length of hospital stay were significantly lower in the FURS group than in the PCNL *** FURS group also had a significantly higher stone clearance rate and a lower incidence of postoperative *** was no significant difference in antibiotic use between the *** serum

关键词： Kidney stones Flexible ureteroscopy Percutaneous nephrolithotomy Clinical effective SCM-1 Erythrocyte sedimentation rate Kidney injury molecule 1

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