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AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis

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Neural Regeneration Research 2025年第8期20卷 2408-2419页

作者： Ruiqi qiu Mingzhu yang Xiuxiu jin jingyang Liu Weiping Wang Xiaoli Zhang jinfeng Han Bo Lei Henan Eye Hospital Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Eye Institute Henan Academy of Innovations in Medical ScienceZhengzhouHenan ProvinceChina Branch of National Clinical Research Center for Ocular Disease Henan Provincial People’s HospitalZhengzhouHenan ProvinceChina Academy of Medical Science Zhengzhou UniversityZhengzhouHenan ProvinceChina

Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell ***,there is no effective treatment for retinitis pigmentosa caused by PDE6B ***-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis *** aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal *** do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and ***-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western ***2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 *** expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type *** Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction ***,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B ***,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B *** data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upr

关键词： apoptosis AAV2-PDE6B ERK1/2 gene therapy phototransduction proteomics rd10 retinitis pigmentosa

Unlocking the stable interface in aqueous zinc-ion battery with multifunctional xylose-based electrolyte additives

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Journal of Energy Chemistry 2025年第1期100卷 770-778页

作者： Xiaoqin Li Jian Xiang Lu qiu Xiaohan Chen Yinkun Zhao Yujue Wang Qu Yue Taotao Gao Wenlong Liu Dan Xiao Zhaoyu jin Panpan Li Institute for Advanced Study Chengdu University School of Mechanical Engineering Chengdu University College of Food and Biological Engineering Chengdu University Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of China College of Materials Science and Engineering Sichuan University

The growth of dendrites and the side reactions occurring at the Zn anode pose significant challenges to the commercialization of aqueous Zn-ion batteries（AZIBs）. These challenges arise from the inherent conflict between mass transfer and electrochemical kinetics. In this study, we propose the use of a multifunctional electrolyte additive based on the xylose（Xylo） molecule to address these issues by modulating the solvation structure and electrode/electrolyte interface, thereby stabilizing the Zn anode. The introduction of the additive alters the solvation structure, creating steric hindrance that impedes charge transfer and then reduces electrochemical kinetics. Furthermore, in-situ analyses demonstrate that the reconstructed electrode/electrolyte interface facilitates stable and rapid Zn2+ion migration and suppresses corrosion and hydrogen evolution reactions. As a result, symmetric cells incorporating the Xylo additive exhibit significantly enhanced reversibility during the Zn plating/stripping process, with an impressively long lifespan of up to 1986 h, compared to cells using pure ZnSO4electrolyte. When combined with a polyaniline cathode, the full cells demonstrate improved capacity and long-term cyclic stability. This work offers an effective direction for improving the stability of Zn anode via electrolyte design, as well as highperformance AZIBs.

关键词： Solvation

团体舞蹈干预对肢体残疾老年人心理影响的实验研究

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浙江体育科学 2019年第3期41卷 74-80页

作者： yanG Yun-jung 司琦金秋艳浙江大学教育学院体育学系浙江杭州310028

针对肢体残疾老年人设计舞蹈干预项目,招募被试50名(65周岁及以上),随机分至实验组(n=25,团体舞蹈项目,60min/次,2次/周)和对照组(n=25,自选身体活动,40～90min/次,1～3次/周),进行为期10周的对照试验。实验前后对身体活动自我效能感(E... 详细信息

针对肢体残疾老年人设计舞蹈干预项目,招募被试50名(65周岁及以上),随机分至实验组(n=25,团体舞蹈项目,60min/次,2次/周)和对照组(n=25,自选身体活动,40～90min/次,1～3次/周),进行为期10周的对照试验。实验前后对身体活动自我效能感(ESE)和社会体格焦虑(SPA)进行测量。配对样本t检验结果显示:对照组ESE和SPA未出现显著变化(t_(ESE)=-1.162,t_(SPA)=-.538;P>.05),效果量小(Cohen’s dESE=0.33,Cohen’s d_(SPA)=0.11);实验组变化显著(t_(ESE)=-8.686,t_(SPA)=-7.703;P<.01),效果量大(Cohen’s d_(ESE)=1.76,Cohen’s d_(SPA)=1.50);重复测量方差分析显示,团体舞蹈干预有效性显著(F_(ESE)=21.217,F_(SPA)=9.835;P<.01),效果量大(ηp■=.371,ηp■=.453)。团体舞蹈有望成为增进肢体残疾老年人心理健康的身体活动项目。

关键词：肢体残疾老年人团体舞蹈自我效能感社会体格焦虑干预

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丙型肝炎病毒企业参考品的建立及初步验证

中国生物制品学杂志 2016年第3期29卷 279-282页

作者：戴峻英伍静邱金燕陈燕清吕馥芬珠海丽珠试剂股份有限公司

目的建立丙型肝炎病毒(hepatitis C virus,HCV)企业参考品,并进行初步验证。方法用6种HCV检测试剂从149份血浆中筛选阳性、阴性样本,建立HCV企业参考品,并对参考品的稳定性和适用性进行验证。结果建立的HCV企业参考品包括30份阳性参考品... 详细信息

目的建立丙型肝炎病毒(hepatitis C virus,HCV)企业参考品,并进行初步验证。方法用6种HCV检测试剂从149份血浆中筛选阳性、阴性样本,建立HCV企业参考品,并对参考品的稳定性和适用性进行验证。结果建立的HCV企业参考品包括30份阳性参考品、30份阴性参考品、4份检测限参考品和1份重复性参考品。参考品的稳定性和适用性良好。结论建立了HCV企业参考品,对本企业的产品质量控制具有重要作用。

关键词：丙型肝炎病毒检测试剂参考品

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日照港石臼港区南作业区翻车机设计选型

水运工程 2016年第10期 133-135,151页

作者：邱田金马岩褚广强崔永鸿日照港集团有限公司山东日照276826 中交水运规划设计院有限公司北京100007

针对日照港石臼港区"东煤南移"的要求,系统分析石臼港区煤炭运量和铁路到港车型情况,并对翻车机适应到港铁路车型情况进行技术经济比选,为该项目的翻车机设计选型提供了科学的决策和支持依据。

关键词：铁路到港车型翻车机设计选型

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甘肃省湿地植被分类系统

湿地科学 2014年第5期12卷 574-579页

作者：马虎生陈学林陶冶金秋燕何莉萍曹文渊中国科学院广西植物研究所广西桂林541006 西北师范大学生命科学学院甘肃兰州7300702 甘肃省野生动植物管理局甘肃兰州730070

系统地分析了甘肃省主要湿地植被类型及其分类。依据中国湿地植被、甘肃植被等分类原则和分类系统,结合甘肃省湿地植被的形成、发育和分布特点,甘肃省湿地植被划分为4个植被型组、9个植被型、74个群系和138个群丛;依据湿地的定义和湿地... 详细信息

系统地分析了甘肃省主要湿地植被类型及其分类。依据中国湿地植被、甘肃植被等分类原则和分类系统,结合甘肃省湿地植被的形成、发育和分布特点,甘肃省湿地植被划分为4个植被型组、9个植被型、74个群系和138个群丛;依据湿地的定义和湿地植被的特征种、指示种等特点,从生态学角度分析西北地区一些受争议的河岸林缘植被、胡杨(Populus euphratica)群系等是否属湿地植被,并依据此类群落的群落和生境特征,将胡杨等群系列入湿地植被。

关键词：湿地植被分类系统河岸植被林缘植被胡杨群系甘肃

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同轴连接器退化对无源互调影响的建模研究

微波学报 2020年第6期36卷 8-13页

作者：石国超毕凌宇赵泓翔金秋延谢军中国电子科技集团公司第五十四研究所石家庄050081 北京邮电大学电子工程学院北京100876

同轴连接器具有轻微的非线性效应,这种非线性效应会引起无源互调干扰。同轴连接器在使用过程中会逐渐退化,使无源互调干扰加重,影响通信系统的可靠性。文中设计并进行了加速退化试验来制备不同退化程度的同轴连接器,在加速退化试验前后... 详细信息

同轴连接器具有轻微的非线性效应,这种非线性效应会引起无源互调干扰。同轴连接器在使用过程中会逐渐退化,使无源互调干扰加重,影响通信系统的可靠性。文中设计并进行了加速退化试验来制备不同退化程度的同轴连接器,在加速退化试验前后,测试了连接器的无源互调性能,并提出了一个新的多项式模型来描述不同退化程度的连接器的无源互调性能,模型与实验结果吻合良好。为分析同轴连接器退化对其无源互调性能的影响提供了理论依据。

关键词：同轴连接器无源互调退化非线性加速退化试验数学建模

Trinuclear gold-catalyzed site-selective alkylation of peptides

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Science China Chemistry 2025年第1期 249-256页

作者： Qing-Yun Fang Chengyihan Gu Yinghan Chen Wencheng yan Yong Liang Weipeng Li Chengjian Zhu Jie Han jin Xie State Key Laboratory of Coordination Chemistry Jiangsu Key Laboratory of Advanced Organic Materials Chemistry and Biomedicine Innovation Center (Chem BIC) School of Chemistry and Chemical Engineering Nanjing University Green Catalysis Center College of Chemistry Zhengzhou University State Key Laboratory of Natural Medicines China Pharmaceutical University

Selective modification of peptides has always been a challenging issue. The selective alkylation of peptides can alter the biological activity and physical properties of molecules, and it has gained significant research interest. We herein disclose an intriguing gold-catalyzed alkylation of peptides using bench-stable unactivated alkyl bromides under photoredox catalysis. A wide range of structurally diverse primary, secondary and tertiary alkyl bromides serve as effective coupling partners to precisely connect with the α-C(sp3)–H in glycine residue of peptides. This protocol demonstrates an exceptionally broad scope for alkyl halides and excellent tolerance for a wide range of useful functional groups. A series of relay transformations to construct cyclic peptides, to proceed click reaction and to realize peptide fluorescence labeling further enhance its synthetic practicality. In addition, mechanistic studies and density functional theory(DFT) calculations reveal an inner-sphere and subsequent outersphere single electron transfer(SET) mechanism.

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超高效液相色谱-蒸发光散射检测法同时测定重楼消瘤方中5种皂苷类成分含量

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分析测试学报 2023年第2期42卷 221-226页

作者：胥爱丽曹颖男张素中邱锦燕谢灿辉毕晓黎肖观林广东省中医药工程技术研究院广东广州510095 广州新华学院药学院广东广州510520 广东省中医药研究开发重点实验室广东广州510095 广州中医药大学第五临床医学院广东广州510405

建立了同时测定抗肿瘤中药复方——重楼消瘤方中重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅶ、柴胡皂苷a、柴胡皂苷d 5种皂苷类成分含量的超高效液相色谱-蒸发光散射检测(UPLC-ELSD)法。复方制剂经甲醇提取,采用Waters Cortecs UPLC C18(150 mm... 详细信息

建立了同时测定抗肿瘤中药复方——重楼消瘤方中重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅶ、柴胡皂苷a、柴胡皂苷d 5种皂苷类成分含量的超高效液相色谱-蒸发光散射检测(UPLC-ELSD)法。复方制剂经甲醇提取,采用Waters Cortecs UPLC C18(150 mm×2.1 mm,1.6μm)色谱柱分离,乙腈-水为流动相进行梯度洗脱,蒸发光散射检测器进行检测,以外标法定量。结果显示,5种皂苷类成分的分离度良好,分别在7.1174~177.94μg/mL、 9.4072~235.18μg/mL、 8.8726~221.82μg/mL、 7.4862~187.15μg/mL、9.761 3~244.03μg/mL范围内线性关系良好,相关系数(r)为0.999 2~0.999 8,检出限为0.7~1.0μg/mL,定量下限为1.8~2.4μg/mL;平均回收率分别为93.9%、103%、102%、104%、105%,相对标准偏差(RSD)分别为3.8%、4.0%、4.2%、3.6%、3.2%。该方法简便、快速、准确,可同时对重楼消瘤方中的5个皂苷类成分进行分析,能有效地消除杂质干扰,为重楼消瘤方的质量评价提供实验依据。

关键词：重楼消瘤方皂苷类成分超高效液相色谱蒸发光散射