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Chinese Journal of Cancer Research 2016年第1期28卷 29-49页

作者： qiu rao qiu-Yuan Xia Liang Cheng Xiao-Jun Zhou Department of Pathology Jinling Hospital Nanjing University School of Medicine Nanjing China Department of Pathology and Laboratory Indiana University School of Medicine Indianapolis IN USA

Renal cell carcinoma （RCC） compromises multiple types and has been emerging dramatically over the recent several decades. Advances and consensus have been achieved targeting common RCCs, such as clear cell carcinoma, papillary RCC and chromophobe RCC. Nevertheless, little is known on the characteristics of several newly-identified RCCs, including clear cell （tubulo） papillary RCC, Xpl 1 translocation RCC, t（6;11） RCC, succinate dehydrogenase （SDH）-deficient RCC, acquired cystic disease- associated RCC, hereditary leiomyomatosis RCC syndrome-associated RCC, ALK translocation RCC, thyroid-like follicular RCC, tubulocystic RCC and hybrid oncocytic/chromophobe tumors （HOCT）. In current review, we will collect available literature of these newly-described RCCs, analyze their clinical pathologic characteristics, discuss their morphologic and immunohistologic features, and finally summarize their molecular and genetic evidences. We expect this review would be beneficial for the understanding of RCCs, and eventually promote clinical management strategies.

关键词： Renal cell carcinoma （RCC） renal tumor immunohistochemistry molecular genetics

上皮样肉瘤10例临床病理及分子遗传学特征研究

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中华病理学杂志 2024年第3期53卷 293-295页

作者：方茹章如松王小桐叶胜兵夏秋媛饶秋解放军东部战区总医院、南京大学医学院附属金陵医院病理科南京210002

目的探讨上皮样肉瘤(ES)的临床病理及分子遗传学特征。方法收集解放军东部战区总医院(南京大学医学院附属金陵医院)2006—2018年病理科共10例ES病例进行回顾性研究,分析其临床病理特征,并利用二代测序检测其相关分子遗传学特征。结果10... 详细信息

目的探讨上皮样肉瘤(ES)的临床病理及分子遗传学特征。方法收集解放军东部战区总医院(南京大学医学院附属金陵医院)2006—2018年病理科共10例ES病例进行回顾性研究,分析其临床病理特征,并利用二代测序检测其相关分子遗传学特征。结果10例ES中,男性7例,女性3例;年龄26~64岁(平均年龄37.3岁)。组织学诊断包括经典型9例、近端型1例。在10例ES标本中,发生SWI/SNF复合物家族基因突变7例,其中SMARCB1拷贝数缺失4例,是最常见的分子遗传学改变。其次,还包括ARID1A基因突变2例、ARID5B基因突变1例、ARID1B基因突变1例及SMARCA4基因突变1例;同时还检测到5例存在PI3K-Akt信号通路的突变。同一个病例亦可同时存在多种SWI/SNF复合物家族基因的异常:其中以SMARCB1和其他基因突变共存较为常见。结论ES有多种形态学亚型,诊断上较为困难,SWI/SNF复合物家族基因突变在ES中频繁出现。其中SMARCB1的占比最高,其失活的分子机制通常为拷贝数缺失。二代测序作为近年来发展起来的一种比较先进的测序方法,可有效筛选相关基因突变,为临床医师提供潜在治疗靶点,从而改善患者预后.

关键词：组织学诊断上皮样肉瘤基因突变回顾性研究临床医师 PI3K 病理科临床病理

基于应变片法的非平行多裂纹相互作用及起裂机理

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Transactions of Nonferrous Metals Society of China 2024年第5期34卷 1655-1668页

作者：沈晴晴黄典一饶秋华易威孙栋良李卓中南大学土木工程学院长沙410075 湖南工学院土木与建筑工程学院衡阳421002

通过计算3条非平行斜裂纹的最大应力强度因子KⅠ,kmax(θ)和KⅡ,kmax(θ),揭示其相互作用机理,并采用多裂纹起裂准则预测非平行多裂纹的起裂参数。对含3条非平行斜裂纹的红砂岩试样开展单轴压缩试验,采用应变片测量方法监测裂纹尖端附... 详细信息

通过计算3条非平行斜裂纹的最大应力强度因子KⅠ,kmax(θ)和KⅡ,kmax(θ),揭示其相互作用机理,并采用多裂纹起裂准则预测非平行多裂纹的起裂参数。对含3条非平行斜裂纹的红砂岩试样开展单轴压缩试验,采用应变片测量方法监测裂纹尖端附近的应变,以确定裂纹起裂顺序。结果表明:裂缝相对倾角的变化对破坏轨迹影响不大且多裂纹尖端很少同时起裂。单轴压缩下含3条非平行斜裂纹红砂岩试件破坏形式可分为两种:翼裂纹贯通和翼裂纹-次生裂纹贯通。断裂机理均为Ⅰ型(拉伸)起裂。预测结果与试验结果吻合较好,验证了理论结果的有效性。

关键词：非平行多裂纹相互作用机理起裂机理应变片法

SEPT9基因甲基化水平在膀胱尿路上皮病变鉴别诊断中的应用

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临床与实验病理学杂志 2024年第4期40卷 373-379页

作者：王更芳周晓莉郭媛饶秋南京医科大学附属常州市第二人民医院病理科常州213003 南京医科大学金陵临床医学院南京210002

目的探讨SEPT9基因甲基化水平在尿路上皮病变良、恶性鉴别诊断中的作用,并尝试通过联合使用SEPT9甲基化检测降低膀胱镜活检组织的假阴性率。方法对160例经病理诊断的尿路上皮病变进行SEPT9甲基化检测,分析其辅助诊断良、恶性病变的敏感... 详细信息

目的探讨SEPT9基因甲基化水平在尿路上皮病变良、恶性鉴别诊断中的作用,并尝试通过联合使用SEPT9甲基化检测降低膀胱镜活检组织的假阴性率。方法对160例经病理诊断的尿路上皮病变进行SEPT9甲基化检测,分析其辅助诊断良、恶性病变的敏感性和特异性;比较癌和癌旁组织间SEPT9基因甲基化水平;在12例首次膀胱镜活检为炎症或良性病变而后续活检、电切为癌的病理组织中分别检测SEPT9基因甲基化水平。结果SEPT9基因甲基化水平鉴别诊断尿路上皮良、恶性病变的敏感性、特异性分别为98%(88/90)和94%(66/70)。47%(7/15)的癌旁组织中SEPT9基因甲基化检测结果为阳性,显示癌旁组织表观遗传修饰异常;12例首次膀胱镜活检为炎症或良性病变的组织中SEPT9基因甲基化检测阳性率为67%(8/12),再次活检、电切病理证实为癌的组织SEPT9甲基化检测阳性率为100%(12/12)。结论SEPT9基因甲基化检测可以辅助尿路上皮病变良、恶性鉴别诊断,并可能有助于减少膀胱镜活检组织的假阴性率。

关键词：尿路上皮病变活检组织鉴别诊断 SEPT9甲基化

HNF-1β在胰腺导管腺癌中的表达及其诊断价值

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诊断病理学杂志 2024年第1期31卷 12-15页

作者：魏雪章如松曹阳程凯王璇马恒辉饶秋方茹东部战区总医院病理科南京210000

目的检测肝细胞核因子1β(hepatocyte nuclear factor 1β,HNF-1β)在胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDAC)组织中的表达并探讨其诊断价值。方法收集2021-01—2023-0149例PDAC,采用免疫组化EnVision两步法检测HNF-1β、CK7、CK20、PAX-8、GATA-3、NapsinA、TTF1、ER、PR、NKX3.1和RCC的表达情况,比较各种肿瘤标记物表达的阳性率。结果HNF-1β阳性率为85.7%(42/49),其中45.2%(19/42)的病例为强阳性,14.3%(6/42)的病例为中等阳性,40.5%(17/42)的病例为弱阳性。CK7阳性率为83.7%(41/49)。CK20阳性率为4.1%(2/49)。PAX-8、GATA-3、Napsin A、TTF1、ER、PR、NKX3.1和RCC均为阴性表达,阳性率为0(0/49)。结论HNF-1β与其他谱系特异性的肿瘤标志物联合使用,可有助于PDAC的诊断和与其他来源的腺癌的鉴别诊断。

关键词：胰腺导管腺癌 HNF-1β 组织芯片免疫组化

Expression of carbonic anhydrase IX and hypoxia-inducible factor 1 alpha in cystic neoplasms of the pancreas

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Expression of carbonic anhydrase IX and hypoxia-inducible fa...

2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青...

作者： Mingming Yuan XiaoYan Ren qiu rao Nantong Maternity and Child Health Care Hospital 东部战区总医院

Background: Accurate distinction of pancreatic cystic neoplasms is essential for pancreatic cancer early detection and prevention as well as avoidance of unnecessary surgical intervention. Serous cystic neoplasms（SCNs） have no malignant potential, but mucinous cystic neoplasms（MCNs） and intraductal papillary mucinous neoplasms（IPMNs） are almost considered malignant or potentially malignant. However, the radiologic distinction between SCNs, MCNs and IPMNs is frequently difficult. Therefore, we hypothesize that identification of biomarkers carbonic anhydrase IX（CA IX）and hypoxia-inducible factor 1 alpha（HIF-1α） combining different clinicopathologic features will improve the differential diagnosis between cystic neoplasms.Methods: 35 SCNs cases including 32 SCAs, 2 macrocystic SCNs and 1 VHL-associated SCN, as well as23 MCNs cases and 14 IPMNs cases were retrospectively reviewed and analyzed. Meanwhile the expressions of CA IX and HIF-1α in cystic neoplasms were detected with immunohistochemistry.Results: Immunohistochemical analysis showed that CA IX was located in cytomembrane. 91.4% SCNs（32/35） were positive for CA IX, including 29 SCAs, 2 macrocystic SCNs and 1 VHL-associated SCN.77.1% SCNs（27/35） were positive for HIF-1α, which was located in nucleus of 24 SCAs and 2 macrocystic SCNs, but 1 VHL-associated SCN in cytoplasm. 95.7%（ 22/23） MCNs and 85.7%（12/14）IPMNs were positive for CA IX. Both MCNs and IPMNs with malignant were positive for CA IX. Compared to SCNs, none of MCNs and IPMNs was positive for HIF-1α（77.1% vs. 0 and 0,all P＜0.001）. IPMNs were significantly more common in the elderly than SCNs（median age 60 vs.48, P=0.001）. MCNs occurred in women more frequently than did SCNs and IPMNs（100% vs. 91.4%and 7.1%, all P ＜ 0.001）. MCNs were usually larger than SCNs（median cyst size 73 vs. 37, P=0.003）.SCNs had a more equal distribution in the pancreas than MCNs and IPMNs. Most MCNs occurred in the body and tail of the pancreas, but IPMNs in contrast. SCNs had a benign clinical course, MCNs and IPMNs could tu

关键词： CA IX HIF-1α cystic neoplasms pancreas

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低级别嗜酸细胞性肾肿瘤14例临床病理分析

临床与实验病理学杂志 2024年第4期40卷 358-362页

作者：刘鑫王璇叶胜兵李学广方茹王小桐章如松陆珍凤饶秋夏秋媛南京大学医学院附属金陵医院/解放军东部战区总医院病理科南京210002

目的探讨低级别嗜酸细胞性肾肿瘤(low-grade oncocytic tumor of kidney,LOT)的临床病理特征、免疫表型、分子改变及鉴别诊断,同时寻找敏感的客观指标辅助诊断。方法收集14例LOT的临床病理资料,行光镜观察、免疫组化染色等,其中8例送检... 详细信息

目的探讨低级别嗜酸细胞性肾肿瘤(low-grade oncocytic tumor of kidney,LOT)的临床病理特征、免疫表型、分子改变及鉴别诊断,同时寻找敏感的客观指标辅助诊断。方法收集14例LOT的临床病理资料,行光镜观察、免疫组化染色等,其中8例送检了高通量靶向基因突变检测。结果14例中男性4例,女性10例,年龄46~88岁,中位年龄57.5岁,平均61.1岁,平均随访6~90个月,1例患者肿瘤复发,其余无瘤生存;镜下肿瘤以实性片状、小巢状结构为主,常兼有大片出血与疏松水肿区域;胞质嗜酸性、细颗粒状,细胞核圆,核仁不明显,常见核周空晕,不易见核皱缩、核多形。免疫表型:LOT免疫表型一致性较好,所有病例均呈现经典的CK7+/CD117-表型(14/14,100%);CD10、CK20、vimentin均呈阴性(0/14,0);Ki67增殖指数1%~3%;此外,新提出的鉴别抗体GPNMB(14/14,100%)弥漫强阳性;GATA3(14/14,100%)至少部分区域中等程度核阳性。高通量测序:8例送检NGS测序,均有MTOR或TSC1基因致病性/可能致病性突变(7例有MTOR基因突变,1例有TSC1基因突变)。结论LOT是一种低级别嗜酸性肿瘤,具有可辨识的形态学特点和免疫表型、分子特征,偏向惰性生物学行为,是一种独立的嗜酸性肿瘤亚型。

关键词：低级别嗜酸细胞性肾肿瘤临床病理免疫组织化学 NGS测序

Effects of COVID-19 pandemic on human fertility:A scientometric and visualized evaluation

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Genes & Diseases 2024年第4期11卷 113-115页

作者： Xiangxiu Wang Yifan rao Lili Tan Ziqiu Hu Lin Wen Weixi Qin Bingyi Li Keqiao He Tieying Yin Juhui qiu Yuming Wang Yaokai Chen Xiancai rao Guixue Wang Key Laboratory of Biorheological and Technology of the Ministry of Education State and Local Joint Engineering LaboratoryforVascular ImplantsModern LifeScience Experiment Teaching Center at Bioengineering College of Chongqing UniversityChongqing 400030China Department of Microbiology College of Basic Medical SciencesKey Laboratory of Microbial Engineering Under the Educational Committee in ChongqingArmy Medical University(Third Military Medical University)Chongqing 400038China Jinfeng Laboratory Chongqing 401329China Division of Infectious Diseases Chongqing Public Health Medical CenterChongqing 400036China

CovID-19,also known as coronavirus disease 2019,is a novel coronavirus disease with high infectivity,strong heterogeneity,and long incubation period(generally 3-14 days).Its main symptoms and signs include fever,dry cough,nasal congestion,fatigue,disorientation,lymphopenia,and dyspnea.The short-term and long-term impacts of covID-19 on human health,particularly its effects on human reproduction and offspring development,continue to receive significant concerns,as they may lead to potential sequelae for several decades or even centuries.

关键词： nasal fertility fever

In vivo imaging reveals a synchronized correlation among neurotransmitter dynamics during propofol and sevoflurane anesthesia

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Zoological Research 2024年第3期45卷 679-690页

作者： Gao-Lin qiu Li-Jun Peng Peng Wang Zhi-Lai Yang Ji-Qian Zhang Hu Liu Xiao-Na Zhu Jin rao Xue-Sheng Liu Department of Anesthesiology First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230022China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China

General anesthesia is widely applied in clinical practice.However,the precise mechanism of loss of consciousness induced by general anesthetics remains unknown.Here,we measured the dynamics of five neurotransmitters,includingγ-aminobutyric acid,glutamate,norepinephrine,acetylcholine,and dopamine,in the medial prefrontal cortex and primary visual cortex of C57BL/6 mice through in vivo fiber photometry and genetically encoded neurotransmitter sensors under anesthesia to reveal the mechanism of general anesthesia from a neurotransmitter perspective.Results revealed that the concentrations of γ-aminobutyric acid,glutamate,norepinephrine,and acetylcholine increased in the cortex during propofol-induced loss of consciousness.Dopamine levels did not change following the hypnotic dose of propofol but increased significantly following surgical doses of propofol anesthesia.Notably,the concentrations of the five neurotransmitters generally decreased during sevoflurane-induced loss of consciousness.Furthermore,the neurotransmitter dynamic networks were not synchronized in the non-anesthesia groups but were highly synchronized in the anesthetic groups.These findings suggest that neurotransmitter dynamic network synchronization may cause anesthetic-induced loss of consciousness.

关键词： General anesthesia Loss of consciousness In vivo neurotransmitter imaging Medial prefrontal cortex Primary visual cortex

Rock fracture under anti-plane shear (Mode Ⅲ) loading

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Journal of Central South University of Technology 2005年第Z1期12卷 125-128页

作者： rao qiu-hua LIAO Zhen-feng School of Civil and Architectural Engineering Central South University Changsha 410083 China School of Civil and Architectural Engineering Central South University Changsha 410083 China

Anti-plane punch-through shear test and anti-planefour-point bending test are used to study the crack initiation and propagation under anti-plane shear (Mode Ⅲ) loading. The tensile and shear stresses at the crack tip are calculated by finite element method. The results show that under Mode Ⅲ loading the maximum principal stress σ1 at crack tip is smaller or a little larger than the maximum shear stress τmax. Since the tensile strength of brittle rock is much lower than its shear strength, σ1 is easy to reach its critical value before τmax reaches its critical value and thus results in Mode I fracture. The fracture trajectory is helicoid and the normal direction of tangential plane with the fractured helicoid is along the predicted direction of the maximum principal stress at the notch tip. It is further proved that Mode Ⅰ instead of Mode Ⅲ fracture occurs in brittle rock under Mode Ⅲ loading. The fracture mode depending on the fracture mechanism must be distinguished from the loading form.

关键词： Mode Ⅲ loading Mode Ⅰ fracture fracture mechanism finite element method rock