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Transcriptional response of Microcystis aeruginosa to the recruitment promoting-benthic bacteria

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Journal of Oceanology and Limnology 2022年第1期40卷 153-162页

作者： Juan WANG Yuande PENG Zhi WANG Wansheng ZOU Xianjin PENG qisheng song College of Life Sciences Hunan Normal UniversityChangsha 410081China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410205China Department of Life Science Hunan University of Arts and ScienceChangde 415000China Division of Plant Sciences University of MissouriColumbia 65211MOUSA

Blooms of Microcystis aeruginosa occur frequently in many freshwater ecosystems around the world,but the mechanism of recovery has not been fully *** our previous study,three benthic bacterial species(***013,***06,and ***24)were identifi ed capable of promoting the recruitment of ***,we further investigated the transcriptional response of *** to the benthic bacteria in early phase of recruitment by means of RNA-Seq *** total,5803803 unigenes on average length of 404 bp were obtained from the transcriptome of *** were 54982 unigenes identifi ed as benthic bacteria-responsive unigenes based on the expression level *** of the protein-protein interaction analysis(PPI)show that the hub genes of the benthic bacteria responsive unigenes mediated network were ribosomal proteins of 30S and 50S,and the most signifi cant functional module of the network was related to the *** the unigenes encoding the translation initiation factors(IF-2,IF-3)and elongation factors(lepA,fusA,and tufA)were up-regulated to respond benthic ***,it indicates that the benthic bacteria have a positive infl uence on activating the ribosome during the early recovery stage of ***.

关键词： Microcystis aeruginosa ribosome benthic bacteria chlorophyll a

G-quadruplex is involved in the regulation of BmSGF1 expression in the Silkworm, Bombyx mori

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Insect Science 2024年第5期31卷 1440-1452页

作者： Yanfei Chen Jin Li Kangkang Niu Yuling Peng qisheng song Qili Feng School of Biology and Agriculture Shaoguan UniversityShaoguanChina Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science Technology School of Life SciencesSouth China Normal UniversityGuangzhouChina and 4Division of Plant Science&TechnologyUniversityofMissouriColumbiaMissouriUSA

Advanced DNA structures,such as the G-quadruplex(G4)and the i-motif,are widely but not randomly present in the genomes of many organisms.A G4 structure was identified in the promoter of the silk gland factor-1 gene(SGFI),which is the main regulatory gene for silk production in Bombyx *** this study,a BmSGF1 G4^(-/-)ho-mozygous mutant was generated with the G4 sequence knocked *** promoter activity of BmSGF1 was lowered in the BmSGF1 G4^(-/-)***(PDS)stabilized the G4 structure and increased the promoter activity of BmSGF1,whereas anti-sense oligonu-cleotide(ASO)complementary to the G4 sequence suppressed the promoter activity of *** with wild-type larvae,the deletion of the BmSGF1 G4 structure de-creased both the expression of BmSGF1 and the fibroin heavy chain gene BmFib-H in the posterior silk gland and the weight of the ***,these results suggest that the promoter G4 structure of BmSGFI participates in the transcription regulation of the BmSGFl gene in the silkworm.

关键词： BmSGF1 G4 Bombyx mori CRISPR/Cas9 silk production

Identification of binding domains and key amino acids involved in the interaction between BmLARK and G4 structure in the BmPOUM2 promoter in Bombyx mori

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Insect Science 2021年第4期28卷 929-940页

作者： Yuling Peng Kangkang Niu Guoxing Yu Mingxi Zheng Qiulan Wei qisheng song Qili Feng Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Division of Plant Sciences University of MissouriColumbiaMOUSA

It has been found that the non-B form DNA structures,like G-quadruplex(G4)and i-motif,are involved in many important biological *** previous study showed that the silkworm transcription factor BmLARK binds to the G4 structure in the promoter of the transcription factor BmPOUM2 and regulates its promoter ***,the binding mechanism between BmLARK and BmPOUM2 G4 structure remains *** this study,binding domains and key amino acid residues involved in the interaction between BmLARK and BmPOUM2 G4 were *** electrophoretic mobility shift assay results indicated that the two RNA-recognition motifs(RRM)of BmLARK are simultaneously required for the binding with the G4 *** RRM1 or RRM2 alone could not bind with the G4 *** zinc-finger motif was not involved in the binding.A series of mutant proteins with specific amino acid mutations were expressed and used to identify the key amino acid residues involving the *** results indicated thatβsheets,especially theβ1 andβ3 sheets,in the RRM domains of BmLARK played critical roles in the binding with the G4 *** amino acid mutations of RRM1/2 in ribonucleoprotein domain 1(RNP1)(motif inβ3 strand)and RNP2(motif inβ1 strand)caused loss of binding ability,indicating that these amino acids are the key sites for the *** the results suggest that RRM domains,particularly their the RNP1 and RNP2 motifs,play important roles not only in RNA recognition,but also in the G4 structure binding.

关键词： binding domains binding protein G4 structure silkworm transcription factor

Copper-ceria sheets catalysts: Effect of copper species on catalytic activity in CO oxidation reaction

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Journal of Rare Earths 2017年第12期35卷 1186-1196页

作者： Linying Du Weiwei Wang Han Yan Xu Wang Zhao Jin qisheng song Rui Si Chunjiang Jia Key Laboratory for Colloid and Interface Chemistry Key Laboratory of Special Aggregated Materials School of Chemistry and Chemical EngineeringShandong University Shanghai Synchrotron Radiation Facility Shanghai Institute of Applied Physics Chinese Academy of Sciences

Copper-ceria sheets catalysts with different loadings of copper（2 wt.%, 5 wt.% and 10 wt.%） supported on ceria nanosheets were synthesized via a depositioneprecipitation（DP） method. The prepared catalysts were systematically characterized with various structural and textural detections including X-ray diffraction（XRD）, Raman spectra, transmission electron microscopy（TEM）, X-ray absorption fine structure（XAFS）, and temperature-programmed reduction by hydrogen（H2-TPR）, and tested for the CO oxidation reaction. Notably, the sample containing 5 wt.% of Cu exhibited the best catalytic performance as a result of the highest number of active CuO species on the catalyst surface. Further increase of copper content strongly affects the dispersion of copper and thus leads to the formation of less active bulk CuO phase, which was verified by XRD and H2-TPR analysis. Moreover, on the basis of in-situ diffuse reflectance infrared Fourier transform spectroscopy（in-situ DRIFTS） results, the surface Cu~＋ species, which are derived from the reduction of Cu^（2＋）, are likely to play a crucial role in the catalyzing CO ***, the superior catalytic performance of the copper-ceria sheets is mainly attributed to the highly dispersed CuOx cluster rather than Cu-[Ox]-Ce structure, while the bulk CuO phase is adverse to the catalytic activity of CO oxidation.

关键词： Ceria nanosheets Copper-ceria catalysts CO oxidation Copper oxide species Structure-properties relationship

Fault Analysis and Innovation in Slag Discharge System of 440-t/h CFB Boiler

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Electricity 2009年第1期20卷 34-37页

作者： Xu qisheng and song Jinghui Guangdong Electric Power Test and Research Institute Wang Ningling Guangdong Electric Power Test and Research Institute

CFB boilers have been widely used in China in recent years with their perfect performances in coal adaptability, load variation capability and lower pollutant emission. The No.3 135-MW CFB unit in Lianzhou Power Plant is the f irst 440-t/h series CFB unit in Guangdong Province. It f inished 72-hour trial operation in Feb. 2004 and was transferred to trial operation. During the trial operation and the next commercial operation, there were some problems happened in the boiler slag discharging system, seriously affecting the safe and reliable operation and the loading capability. After innovation, these problems have been completely solved. Hopefully the solutions may be used for reference to the units with similar problems.

关键词： CFB Fault Analysis and Innovation in Slag Discharge System of 440-t/h CFB Boiler Innovation

高精度电离层产品增强PPP-B2b定位性能分析

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武汉大学学报（信息科学版） 2024年第9期49卷 1517-1526页

作者：宋伟伟宋啟晟何倩倩龚晓鹏辜声峰武汉大学卫星导航定位技术研究中心湖北武汉430079 武汉大学湖北珞珈实验室湖北武汉430079 中国电子科技集团第15研究所北京100083

北斗三号卫星导航系统(BeiDou-3 satellite navigation system,BDS-3)通过PPP-B2b信号向中国及周边区域提供实时精密单点定位(precise point positioning,PPP)服务。由于PPP-B2b信号未提供电离层模型产品,目前的定位研究主要采用无电离... 详细信息

北斗三号卫星导航系统(BeiDou-3 satellite navigation system,BDS-3)通过PPP-B2b信号向中国及周边区域提供实时精密单点定位(precise point positioning,PPP)服务。由于PPP-B2b信号未提供电离层模型产品,目前的定位研究主要采用无电离层组合,其精度可以到达分米级,但是需要30 min左右的收敛时间。针对PPP-B2b定位收敛时间长的问题,分析了电离层模型产品对定位收敛性能的提升。将准四维电离层建模方法引入中国区域电离层延迟建模,并采用2021年年积日第201—207天中国区域均匀分布的50、100、150和200个基准站4种方案进行解算,结果表明,当电离层建模所用测站数量从50个增加至200个时,电离层模型产品精度从2~3 TECU提升至优于0.8 TECU。高精度电离层模型产品可以显著缩短PPP-B2b定位收敛时间,相比于目前PPP-B2b定位,基于电离层模型产品增强的单BDS-3动态PPP收敛时间仅需7.56 min,缩短77%;GPS+BDS-3双系统动态PPP收敛时间由13.51 min缩短至4.45 min。因此,高精度电离层模型产品能显著缩短PPP-B2b的定位收敛时间。

关键词：电离层模型北斗三号卫星导航系统 PPP-B2b 精密单点定位快速收敛

Insect Neuropeptide Bursicon Homodimers Induce Innate Immune and Stress Genes during Molting by Activating the NF-kB Transcription Factor Relish

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Insect Neuropeptide Bursicon Homodimers Induce Innate Immune...

首届“全国青年昆虫学科技工作者创新论坛”

作者： Shiheng An Shengzhang Dong Qian Wang Sheng Li Lawrence I.Gilbert David Stanley qisheng song Division of Plant Sciences University of MissouriColumbiaMissouriUSA Shanghai Institute of Plant Physiology and Ecology Chinese Academy of SciencesShanghaiChina Department of Biology University of North CarolinaChapel HillNorth CarolinaUSA USDA/Agricultural Research Service Biological Control of Insects Research LaboratoryColumbiaMissouriUSA

Bursicon is a heterodimer neuropeptide composed of two cystine knot proteins, bursicon a(burs a)and bursicon b(burs b),that elicits cuticle tanning(melanization and sclerotization)through the Drosophila leucine-rich repeatscontaining G protein-coupled receptor 2(DLGR2).Recent studies show that both bursicon subunits also form ***,biological functions of the homodimers have remained unknown until *** the present study,we show in Drosophila melanogaster that both bursicon homodimers induced expression of genes encoding antimicrobial peptides(AMPs)in

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非标准设备驱动程序与微机DOS的接口

山东大学学报（自然科学版） 1997年第2期32卷 184-191页

作者：樊永华山东大学电子工程系

阐述了一种与ＤＯＳ接口的非标准设备驱动程序的编程方法，它增加了非标准设备驱动程序的适用性．

关键词：字符设备设备驱动程序设备链接口微机 DOS

基于“三全育人”理念的大学化学课程思政案例构建与实践

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大学化学 2021年第3期36卷 55-60页

作者：戴昉纳宋其圣中国石油大学(华东)材料科学与工程学院山东青岛266580 山东大学化学与化工学院济南250100

对部分大学化学课程的教学理念和教学内容进行改革和探索。通过知识传授与价值引领双轨并行,构建了以大学化学知识为主体,以提升大学生的家国人文情怀、科学文化素养、科学思维方法为主导的大学化学课程的思政教学体系。通过与课程知识... 详细信息

对部分大学化学课程的教学理念和教学内容进行改革和探索。通过知识传授与价值引领双轨并行,构建了以大学化学知识为主体,以提升大学生的家国人文情怀、科学文化素养、科学思维方法为主导的大学化学课程的思政教学体系。通过与课程知识的有机结合,开展爱国主义、哲学辩证思想、认识发展观、科学素养、品格意志力、社会规则秩序和安全意识等七个育人思想,对大学化学课程思政的设计理念、具体案例和实施方法进行介绍。

关键词：大学化学课程思政教学改革教学案例