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Lgi1基因通过控制髓鞘装配和TSC1-mTORC1依赖的脂质生物合成促进外周神经系统髓鞘化

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遵义医科大学学报 2024年第1期47卷 10-17页

作者：唐涣垚易如岚梁小玲王旭冉啟鹏周亮遵义医科大学脑科学特色重点实验室贵州遵义563099 遵义医科大学附属医院麻醉科贵州遵义563099 遵义医科大学贵州省麻醉与器官保护基础研究重点实验室贵州遵义563099

目的探索富亮氨酸胶质瘤失活基因1(Lgi1)在外周神经系统(PNS)髓鞘化的作用及其分子机制。方法应用蛋白质印迹法检测Lgi1与髓鞘相关蛋白在产后发育时期SN组(坐骨神经)P3-P60、Brain组(大脑)P0-P180、敲除Lgi1后SN组与Brain组的表达,并... 详细信息

目的探索富亮氨酸胶质瘤失活基因1(Lgi1)在外周神经系统(PNS)髓鞘化的作用及其分子机制。方法应用蛋白质印迹法检测Lgi1与髓鞘相关蛋白在产后发育时期SN组(坐骨神经)P3-P60、Brain组(大脑)P0-P180、敲除Lgi1后SN组与Brain组的表达,并进一步探索Lgi1-/-小鼠Laminin-integrin信号及TSC1-mTORC1通路相关蛋白表达情况;利用免疫组织化学与电镜探究敲除Lgi1后引起的髓鞘超微结构改变。结果蛋白质印迹法显示,Lgi1的缺失会导致PNS中髓鞘相关蛋白MBP、MOG和MAG与特异性髓鞘蛋白MPZ的表达水平下降(P<0.05),且电镜下实验组小鼠SN中无髓鞘的轴突数量增加,而轴突总数不变,g-ratio比率增加,且未折叠髓鞘增加;Lgi1-/-小鼠SN中Laminin、integrinβ-1、integrinβ-4蛋白表达明显减少,integrin α-1表达增加(P<0.05);免疫荧光染色结果显示,MBP与Laminin共染部分较少;此外,与对照组相比,Lgi1-/-小鼠中脂肪酸合成酶FASN蛋白表达量减少,pS6水平增加和TSC1表达减少(P<0.05)。结论 Lgi1基因参与调控PNS髓鞘化过程,且通过Laminin-integrin信号及TSC1-mTORC1通路抑制髓鞘的形成并导致PNS低髓鞘化。

关键词：富亮氨酸胶质瘤失活基因1 髓鞘化髓鞘装配脂质合成雷帕霉素靶蛋白

Topology optimization of AISI 4140 steel with surface texture filled by multi-solid lubricants for enhancing tribological properties

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Friction 2024年第9期12卷 2033-2051页

作者： qipeng HUANG Chaohua WU Xiaoliang SHI Kaipeng ZHANG School of Mechanical and Electronic Engineering Wuhan University of TechnologyWuhan 430070China School of Transportation and Logistics Engineering Wuhan University of TechnologyWuhan 430063China

Wind power gears will be excessively worn due to lubrication failure during ***,the tribological properties of rubbing pairs are improved by filling solid lubricants into surface *** texture design,three types of topological textures(Circle(C),Hexagon(H),and Circle/Hexagon(CH))were obtained by cell topology optimization,and then three cases with 20%,30%,and 40%density were designed for each ***,SnAgCu and TiC were deposited in texture of AISI 4140 steel(AS)to obtain 9 kinds of self-lubricating *** them,AS with 30%CH density(AS-CH30)exhibits excellent mechanical and tribological *** with AS-C and AS-H,the maximum equivalent stress of AS-CH was decreased by 10.86%and 5.37%,*** coefficient and wear rate of AS-CH30 were 79.68%and 78%lower than those of *** excellent tribological performances of AS-CH30 can be attributed to the synergistic effect of topological surface and solid *** surface can not only reduce fluctuation of equivalent stress,but also promote the stored lubricants to be easily transferred at the contact interface to form a 200 nm lubricating film containing solid lubricants(mainly),oxides and wear debris.

关键词： topological texture nano-TiC self-lubricating solid lubrication

Multi-Time-Scale Resource Allocation Based on Long-Term Contracts and Real-Time Rental Business Models for Shared Energy Storage Systems

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Journal of Modern Power Systems and Clean Energy 2024年第2期12卷 454-465页

作者： Yuxuan Zhuang Zhiyi Li qipeng Tan Yongqi Li Minhui Wan College of Electrical Engineering Zhejiang UniversityHangzhouChina China Southern Power Grid Power Generation Company Energy Storage Research Institute GuangzhouChina IEEE

The push for renewable energy emphasizes the need for energy storage systems(ESSs)to mitigate the unpre-dictability and variability of these sources,yet challenges such as high investment costs,sporadic utilization,and demand mismatch hinder their broader *** response,shared energy storage systems(SESSs)offer a more cohesive and efficient use of ESS,providing more accessible and cost-effective energy storage solutions to overcome these *** enhance the profitability of SESSs,this paper designs a multi-time-scale resource allocation strategy based on long-term contracts and real-time rental business *** initially construct a life cycle cost model for SESS and introduce a method to estimate the degradation costs of multiple battery groups by cycling numbers and depth of discharge within the ***,we design various long-term contracts from both capacity and energy perspectives,establishing associated models and real-time rental ***,multi-time-scale resource allocation based on the decomposition of user demand is *** analysis validates that the business model based on long-term contracts excels over models operating solely in the real-time market in economic viability and user satisfaction,effectively reducing battery degradation,and leveraging the aggregation effect for SESS can generate an additional increase of 10.7%in net revenue.

关键词： Capacity allocation long-term contracts shared energy storage system stochastic programming

Synthetic auxotrophs accelerate cell factory development through growth-coupled models

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Frontiers of Chemical Science and Engineering 2024年第9期18卷 73-87页

作者： Liangpo Li Linwei Yu Xinxiao Sun qipeng Yuan Xiaolin Shen Jia Wang State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing 100029China

The engineering of microbial cell factories for the production of high-value chemicals from renewable resources presents several challenges,including the optimization of key enzymes,pathway fluxes and metabolic *** these challenges involves the development of synthetic auxotrophs,a strategy that links cell growth with enzyme properties or biosynthetic *** linkage allows for the improvement of enzyme properties by in vivo directed enzyme evolution,the enhancement of metabolic pathway fluxes under growth pressure,and remodeling of metabolic networks through directed strain *** advantage of employing synthetic auxotrophs lies in the power of growth-coupled selection,which is not only high-throughput but also labor-saving,greatly simplifying the development of both strains and *** auxotrophs play a pivotal role in advancing microbial cell factories,offering benefits from enzyme optimization to the manipulation of metabolic networks within single ***,this strategy extends to coculture systems,enabling collaboration within microbial *** review highlights the recently developed applications of synthetic auxotrophs as microbial cell factories,and discusses future perspectives,aiming to provide a practical guide for growth-coupled models to produce value-added chemicals as part of a sustainable biorefinery.

关键词： synthetic auxotrophs growth-coupled directed enzyme evolution pathway flux directed strain evolution coculture

Micromechanical modeling of longitudinal tensile behavior and failure mechanism of unidirectional carbon fiber/aluminum composites involving fiber strength dispersion

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Chinese Journal of Aeronautics 2024年第9期37卷 312-327页

作者： qipeng LIU Wengang JIANG Yuehua GAO Zhenjun WANG Shanshan SHI Zhi SUN Jiangxi Key Laboratory of Forming and Joining Technology for Aerospace Components Nanchang Hangkong UniversityNanchang 330063China School of Locomotive and Rolling Stock Engineering Dalian Jiaotong UniversityDalian 116028China State Key Laboratory of Structural Analysis Optimization and CAE Software for Industrial EquipmentDalian University of TechnologyDalian 116024China

This paper examines the longitudinal tensile behavior and failure mechanism of a new unidirectional carbon fiber reinforced aluminum composite through experiments and simulations.A Weibull distribution model was established to describe the fiber strength dispersion based on single-fiber tensile tests for carbon fibers extracted from the *** constitutive models for the matrix and interface were established based on the uniaxial tensile and single-fiber push-out tests,***,a 3D micromechanical numerical model,innovatively considering the fiber strength dispersion by use of the weakest link and Weibull distribution theories,was estab-lished to simulate the progressive failure behavior of the composite under longitudinal *** to the dispersion of fiber strength,the weakest link of the fiber first fractures,and stress concentra-tion occurs in the surrounding fibers,interfaces,and *** maximum stress concentration fac-tor for neighboring fibers varies nonlinearly with the distance from the fractured *** isolated and clustered fractured fibers are present during the progressive failure process of the *** expansion of fractured fiber clusters intensifies stress concentration and material degradation which in turn enlarges the fractured fiber clusters,and their mutual action leads to the final collapse of the composite.

关键词： Fiber reinforced metals Finite element method 3D micromechanical model Fiber strength dispersion Longitudinal tension Progressive failure

New process for treating boron-bearing iron ore by flash reduction coupled with magnetic separation

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International Journal of Minerals,Metallurgy and Materials 2024年第3期31卷 473-484页

作者： qipeng Bao Lei Guo Hong Yong Sohn Haibin Zuo Feng Liu Yongliang Gao Zhancheng Guo State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China Department of Materials Science&Engineering University of UtahUtah 84112USA China ENFI Engineering Corporation Beijing 100038China

Boron is an important industrial raw material often sourced from minerals containing different compounds that cocrystallize,which makes it difficult to separate the mineral phases through conventional *** study proposed a new treatment called flash reduction-melting separation(FRMS)for boron-bearing iron *** this method,the concentrates were first flash-reduced at the temperature under which the particles melt,and the slag and the reduced iron phases disengaged at the particle *** reduc-tion and melting effects were achieved above 1550℃.The B_(2)O_(3) content in the separated slag was over 18wt%,and the B content in the iron was less than 0.03wt%.The proposed FRMS method was tested to investigate the effects of factors such as ore particle size and tem-perature on the reduction and melting steps with and without pre-reducing the raw *** mineral phase transformation and morphology evolution in the ore particles during FRMS were also comprehensively analyzed.

关键词： ludwigite boron-bearing iron concentrate flash reduction melting separation boron

GST family genes in jujube actively respond to phytoplasma infection

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Horticultural Plant Journal 2024年第1期10卷 77-90页

作者： qipeng Wang Liman Zhang Chaoling Xue Yao Zhang Xiangrui Meng Zhiguo Liu Mengjun Liu Jin Zhao College of Life Science Hebei Agricultural UniversityBaodingHebei 071000China Hebei Key Laboratory of Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaodingHebei 071000China Research Center of Chinese Jujube Hebei Agricultural UniversityBaodingHebei 071000China

Jujube witches’broom(JWB)caused by phytoplasma has a severely negative effect on multiple metabolisms in *** GST gene family in plants participates in the regulation of a variety of biotic and abiotic *** study aims to identify and reveal the changes in the jujube GST gene family in response to phytoplasma ***,70 ZjGSTs were identified in the jujube genome and divided into 8 *** them,the Tau-class,including 44 genes,was the *** analysis indicated that Tau-class genes were highly conserved among species,such as Arabidopsis,cotton,chickpea,and *** chromosome location analysis,37.1%of genes were clustered,and 8 of 9 gene clusters were composed of Tau class *** RT-PCR,qRT-PCR and enzyme activity detection,the results showed that the expression of half(20/40)of the tested ZjGSTs was inhibited by phytoplasma infection in field and tissue culture conditions,and GST activity was also significantly *** the resistant and susceptible varieties under phytoplasma infection,ZjGSTU49-ZjGSTU54 in the cluster IV showed opposite expression patterns,which may be due to functional divergence during *** upregulated genes(ZjGSTU45,ZjGSTU49,ZjGSTU59,and ZjGSTU70)might be involved in the process of jujube against *** yeast two-hybrid results showed that all 6 Tauclass proteins tested could form homodimers or ***,the comprehensive analysis of the jujube GST gene family revealed that ZjGSTs responded actively to phytoplasma ***,some screened genes(ZjGSTU24,ZjGSTU49-52,ZjGSTU70,and ZjDHAR10)will contribute to further functional studies of jujube-phytoplasma interactions.

关键词： Chinese jujube GST gene Family Phytoplasma Gene cluster Expression Protein interaction