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3,6-dichlorobenzo[b]thiophene-2-carboxylic acid alleviates ulcerative colitis by suppressing mammalian target of rapamycin complex 1 activation and regulating intestinal microbiota

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World Journal of Gastroenterology 2022年第46期28卷 6522-6536页

作者： Qiong-Zi He Peng Wei Jun-Zhi Zhang Tong-Tong Liu Kun-Qun Shi Huan-Huan Liu Jing-Wei Zhang Shi-Jia Liu Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese MedicineNanjing 210029Jiangsu ProvinceChina College of The First Clinical Medicine Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina School of Life Science and Technology China Pharmaceutical UniversityNanjing 211198Jiangsu ProvinceChina

BACKGROUND 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid(BT2)is a benzothiophene carboxylate derivative that can suppress the catabolism of branched-chain amino acid(BCAA)-associated mammalian target of rapamycin complex 1(mTORC1)activation.Previous studies have demonstrated the therapeutic effects of BT2 on arthritis,liver cancer,and kidney injury.However,the effects of BT2 on ulcerative colitis(UC)are unknown.AIM To investigate the anti-UC effects of BT2 and the underlying mechanism.METHODS Mouse UC models were created through the administration of 3.5%dextran sodium sulfate(DSS)for 7 d.The mice in the treated groups were administered salazosulfapyridine(300 mg/kg)or BT2(20 mg/kg)orally from day 1 to day 7.At the end of the study,all of the mice were sacrificed,and colon tissues were removed for hematoxylin and eosin staining,immunoblot analyses,and immunohistochemical assays.Cytokine levels were measured by flow cytometry.The contents of BCAAs including valine,leucine,and isoleucine,in mouse serum were detected by liquid chromatography-tandem mass spectrometry,and the abundance of intestinal flora was analyzed by 16S ribosomal DNA sequencing.RESULTS Our results revealed that BT2 significantly ameliorated the inflammatory symptoms and pathological damage induced by DSS in mice.BT2 also reduced the production of the proinflammatory cytokines interleukin 6(IL-6),IL-9,and IL-2 and increased the anti-inflammatory cytokine IL-10 level.In addition,BT2 notably improved BCAA catabolism and suppressed mTORC1 activation and cyclooxygenase-2 expression in the colon tissues of UC mice.Furthermore,highthroughput sequencing revealed that BT2 restored the gut microbial abundance and diversity in mice with colitis.Compared with the DSS group,BT2 treatment increased the ratio of Firmicutes to Bacteroidetes and decreased the abundance of Enterobacteriaceae and Escherichia-Shigella.CONCLUSION Our results indicated that BT2 significantly ameliorated DSS-induced UC and that the latent mechanism involved the suppression of BCAA-as

关键词： 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid Ulcerative colitis Mechanistic target of rapamycin complex 1 Intestinal flora Dextran sodium sulfate Cyclooxygenase-2

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Specific activation of 2'-5'oligoadenylate synthetase gene promoter by hepatitis C virus-core protein:A potential for developing hepatitis C virus targeting gene therapy

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World Journal of Gastroenterology 2009年第25期15卷 3178-3182页

作者： Ying Wang Shan-Shan Mao qiong-qiong he Yuan zi Ji-Fang Wen De-Yun Feng Department of Pathology College of Basic Medical Sciences Central South University Changsha 410078 Hunan Province China Department of Pathology Shanghai Medical College Fudan University Shanghai 200032 China

AIM:To examine whether 2'-5'oligoadenylate synthetase (OAS) gene promoter can be specifically activated by hepatitis C virus (HCV)-core protein.METHODS: Human embryo hepatic cell line L02 wa transfected with pcDNA3.1-core plasmid and selected by G418. Expression of HCV-core was detected by reverse transcription polymerase chain reaction and Western blotting. The OAS promoter sequence wa amplified from the genomic DNA and inserted into pGL3-basic vector. The resultant pGL3-OAS-Luc plasmid was transiently transfected into L02/core cell and luciferase activity was assayed. RESULTS: L02/core cell line stably expressing HCV core protein was established. The pGL3-OAS-Luc construct exhibited significant transcriptional activity in the L02/core cells but not in the L02 cells.CONCLUSION: HCV-core protein activates the OAS gene promoter specifically and effectively. Utilization of OAS gene promoter would be an ideal strategy for developing HCV-specific gene therapy.

关键词：丙型肝炎病毒基因启动子核心蛋白基因治疗合成酶激活逆转录聚合酶链反应 pcDNA3.1

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Different nutrient compositions in diet and taking hypoglycemic drugs can modulate gut microbial flora

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World Journal of Diabetes 2022年第9期13卷 799-801页

作者： zi-Jun Lin qiong-Wen Zhang Xiao-Lan Yu Bo Zhou Chang-Wei Liu Lian-Ping he School of Medicine Taizhou UniversityJiaojiang 318000Zhejiang ProvinceChina Children’s Hospital of Nanjing Medical University Nanjing 210008Jiangsu ProvinceChina

The diet structure of diabetic patients is different from that of normal people.Diabetic patients also need to take hypoglycemic drugs to regulate blood sugar.Both dieting and drugs affect the gut microbiota of diabetic patients.In this letter,we discuss that different dietary patterns and the use of hypoglycemic agents may have an impact on changes in gut microbiota in diabetic patients.

关键词： Diabetic patients Gut microbiota Hypoglycemic drugs

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Multi-channel phasemeter and its application in the heterodyne laser interferometry

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Science China(Technological Sciences) 2015年第4期58卷 746-749页

作者： LIU he Shan DONG Yu Hui LUO zi Ren LI Yu qiong JIN Gang National Microgravity Laboratory (NML) Institute of Mechanics Chinese Academy of Sciences School of Physics Chinese Academy of Sciences

The mission to detect gravitational wave in space requires a not only sophisticated but also ultra-precise laser interferometric measurement system.Within a single spacecraft,tens of interferometric beat signals are generated at the same time and they also need to be processed simultaneously.In this paper,a multi-channel phasemeter which can parallelly process the signals is constructed.The test shows that a sensitivity of 2Π rad/√Hz could be achieved in the frequency range of 0.1 to 10 Hz.We also utilize the phasemeter to evaluate the performance of a heterodyne laser interferometer.

关键词：外差激光干涉仪多通道应用干涉测量系统太空任务频率范围引力波超精密

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新工科多元融合实习教学模式探索——以中国矿业大学矿物加工工程专业为例

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教育教学论坛 2023年第23期 89-92页

作者：廖寅飞贺琼琼马子龙中国矿业大学化工学院江苏徐州221116

基于新工科建设理念,紧扣新工科教育和工程专业认证要求,从矿物加工工程专业实习教学模式现状和存在的问题出发,以多层次、多阶段和多维度深度融合为思路,探索构建了课程思政、安全教育、线上教学、线下实训和虚拟仿真五位一体的多元融... 详细信息

基于新工科建设理念,紧扣新工科教育和工程专业认证要求,从矿物加工工程专业实习教学模式现状和存在的问题出发,以多层次、多阶段和多维度深度融合为思路,探索构建了课程思政、安全教育、线上教学、线下实训和虚拟仿真五位一体的多元融合实习教学模式。实践证明,多元融合实习教学模式能够有效激发学生对专业的学习兴趣,提升学生精神面貌、认真程度和综合能力,提高实习教学效果,保障了“双一流”专业的实习教学质量。

关键词：新工科多元融合虚拟仿真教学模式矿物加工

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Exenatide once weekly combined with metformin reduced glycemic variability in type 2 diabetes by using flash glucose monitoring system

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World Journal of Diabetes 2020年第12期11卷 654-665页

作者： Yang Li Min-Min Han qiong he zi-Ang Liu Dong Liang Jing-Tian Hou Yi Zhang Yun-Feng Liu Department of Endocrinology First Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of First Medical College Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Pharmacology Basic Medical CollegeShanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina

BACKGROUND Multiple studies demonstrate that fluctuating blood glucose level produces greater damage compared with sustained hyperglycemia.Flash glucose monitoring system is an effective method in documenting blood glucose variability,contributing to better glucose management and reduced hypoglycemic event occurrence.AIM To investigate the improvement in glycemic variability(GV),blood glucose level,and metabolic indexes of patients with type 2 diabetes mellitus after combined treatment of exenatide once weekly(EXQW)and metformin.METHODS Twenty-five patients with type 2 diabetes mellitus suffering from poor blood glucose control under metformin treatment were recruited.The recruited patients were prescribed with oral metformin only(maintaining a dosage of metformin at≥1500 mg/day)for 2 wk(screening period),and then given EXQW(2 mg,subcutaneous injection)for 12 wk(experimental period).The flash glucose monitoring system was used to document blood glucose values during the screening period and the last 2 wk of the experimental period.RESULTS Four patients were excluded for various reasons,yielding a total of 21 patients,including 17 males and 4 females,with an average age of 48.8 years,who completed this study.The estimated glycated hemoglobin,mean blood glucose,fasting and postprandial blood glucose levels,and percentage of blood glucose above 7.8 mmol/L decreased compared to those at baseline(P=0.003,0.003,0.008,0.010,0.014,0.017,and 0.005,respectively),while the percentage of blood glucose between 3.9 and 7.8 mmol/L significantly increased(P=0.005).Parameters of GV including standard deviation of blood glucose,mean amplitude of glycemic excursions,mean of daily difference,area under the curve difference between percentiles 25 and 75,and area under the curve difference between percentiles 10 and 90 were significantly lower compared to that of baseline(P=0.017,0.006,0.000,0.024,0.036,respectively).The durations of blood glucose below 3.9 mmol/L during the day and nocturnal periods significantly increased after trea

关键词： Flash glucose monitoring Glycemic variability Type 2 diabetes mellitus Exenatide once weekly

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Prognostic value and predictive threshold of tumor volume for patients with locally advanced nasopharyngeal carcinoma receiving intensity-modulated radiotherapy

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Chinese Journal of Cancer 2016年第12期35卷 725-734页

作者： Yu-Xiang he Ying Wang Peng-Fei Cao Lin Shen Ya-Jie Zhao zi-Jian Zhang Deng-Ming Chen Tu-Bao Yang Xin-qiong Huang Zhou Qin You-Yi Dai Liang-Fang Shen Department of Oncology Xiangya HospitalCentral South University Department of hematology Xiangya HospitalCentral South University Department of Radiology Xiangya HospitalCentral South University School of Public health Central South University

Background: Gross target volume of primary tumor(GTV?P) is very important for the prognosis prediction of patients with nasopharyngeal carcinoma(NPC), but it is unknown whether the same is true for locally advanced NPC patients treated with intensity?modulated radiotherapy(IMRT). This study aimed to clarify the prognostic value of tumor volume for patient with locally advanced NPC receiving IMRT and to ind a suitable cut?of value of GTV?P for prognosis prediction.Methods: Clinical data of 358 patients with locally advanced NPC who received IMRT were reviewed. Receiver oper?ating characteristic(ROC) curves were used to identify the cut?of values of GTV?P for the prediction of diferent end?points [overall survival(OS), local relapse?free survival(LRFS), distant metastasis?free survival(DMFS), and disease?free survival(DFS)] and to test the prognostic value of GTV?P when compared with that of the American Joint Committee on Cancer T staging system.Results: The 358 patients with locally advanced NPC were divided into two groups by the cut?of value of GTV?P as determined using ROC curves: 219(61.2%) patients with GTV?P ≤46.4 mL and 139(38.8%) with GTV?P >46.4 mL. The 3?year OS, LRFS, DMFS, and DFS rates were all higher in patients with GTV?P ≤46.4 mL than in those with GTV?P > 46.4 mL(all P 46.4 mL was an independent unfavorable prognostic factor for patient survival. The ROC curve veriied that the predictive ability of GTV?P was superior to that of T category(P he cut?of values of GTV?P for the prediction of OS, LRFS, DMFS, and DFS were 46.4, 57.9, 75.4 and 46.4 mL, respectively.Conclusion: In patients with locally advanced NPC, GTV?P >46.4 mL is an independent unfavorable prognostic indi?cator for survival after IMRT, with a prognostic value superior to that of T category.

关键词： Nasopharyngeal carcinoma Intensity-modulated radiotherapy Gross target volume of primary tumor Prognosis

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Co-infection of SARS-COV-2 and Influenza A Virus:A Case Series and Fast Review

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Current Medical Science 2021年第1期41卷 51-57页

作者： Xuan XIANG zi-hao WANG Lin-lin YE Xin-liang he Xiao-shan WEI Yan-ling MA Hui LI Long CheN Xiao-rong WANG qiong ZHOU Department of Respiratory and Critical Care Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Coronavirus disease 2019(COVID-19)occurs in the influenza season and has become a global pandemic.The present study aimed to examine severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)co-infection with influenza A virus(IAV)in an attempt to provide clues for the antiviral interventions of co-infected patients.We described two patients who were co-infected with SARS-CoV-2 and IAV treated at Wuhan Union Hospital,China.In addition,we performed a review in PubMed,Web of Science and CNKI(from January 1 up to November 1,2020)with combinations of the following key words:“COVID-19,SARS-COV-2,influenza A and co-infection”.A total of 28 co-infected patients were enrolled in the analysis.Of the 28 patients,the median age was 54.5 years(IQR,34.25–67.5)and 14 cases(50.0%)were classified as severe types.The most common symptoms were fever(85.71%),cough(82.14%)and dyspnea(60.71%).Sixteen patients had lymphocytopenia on admission and 23 patients exhibited abnormal radiological changes.The median time from symptom onset to hospital admission was 4 days(IQR,3–6),and the median time of hospital stay was 14 days(IQR,8.5–16.75).In conclusion,patients with SARSCOV-2 and IAV co-infection were similar to those infected with SARS-COV-2 alone in symptoms and radiological images.SARS-COV-2 co-infection with IAV could lead to more severe clinical condition but did not experience longer hospital stay compared with patients infected with SARSCOV-2 alone.

关键词： co-infection COVID-19 influenza A SARS-COV-2

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Kaempferol inhibits Pseudorabies virus replication in vitro through regulation of MAPKs and NF-κB signaling pathways

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Journal of Integrative Agriculture 2021年第8期20卷 2227-2239页

作者： CheN Xu CheN Ya-qin YIN Zhong-qiong WANG Rui HU Huai-yue LIANG Xiao-xia he Changliang YIN Li-zi YE Gang ZOU Yuan-feng LI Li-xia TANG Hua-qiao JIA Ren-yong SONG Xu Natural Medicine Research Center College of Veterinary MedicineSichuan Agricultural UniversityChengdu 611100P.R.China

Pseudorabies virus(PRV),in the family herpesviridae,is a pathogen of Aujeszky’s disease,which causes great economic losses to the pig industry.Recent outbreaks of Pseudorabies imply that new control measures are urgently needed.The present study shows that kaempferol is a candidate drug for controlling PRV infection,as it possesses the ability to inhibit PRV replication in a dose-dependent manner in vitro.Kaempferol at a concentration of 52.40μmol L^(-1) could decrease PRV-induced cell death by 90%.With an 50%inhibitory concentration(IC50)value of 25.57μmol L^(-1),kaempferol was more effective than acyclovir(positive control)which has an IC50 value of 54.97μmol L^(-1).A mode of action study indicated that kaempferol inhibited viral penetration and replication stages,decreasing viral loads by 4-and 30-fold,respectively.Addition of kaempferol within 16 h post infection(hpi)could significantly inhibit virus replication,and viral genome copies were decreased by almost 15-fold when kaempferol was added at 2 hpi.Kaempferol regulated the NF-κB and MAPKs signaling pathways involved in PRV infection and changed the levels of the target genes of the MAPKs(ATF-2 and c-Jun)and NF-κB(IL-1α,IL-1βand IL-2)signaling pathways.The findings of the current study suggest that kaempferol could be an alternative measure to control PRV infection.

关键词： kaempferol antiviral activity Pseudorabies virus

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5/6肾脏切除动物疾病模型的中医证候探索及扶正化瘀降浊通络方证测验

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中国中医基础医学杂志 2021年第11期27卷 1717-1720,1748页

作者：陈紫薇陈明何凯杰陈艳琼成都市中西医结合医院成都610000 成都中医药大学附属医院成都610000

目的:探讨5/6肾切除大鼠的中医证型及用扶正化瘀降浊通络方进行方证测验。方法:将雄性SD大鼠随机分为假手术组、模型组、中药组(给予扶正化瘀降浊通络方),采用“5/6肾切除法”造模,动态观察一般情况,给药后4、8、12周通过检测免疫指标CD... 详细信息

目的:探讨5/6肾切除大鼠的中医证型及用扶正化瘀降浊通络方进行方证测验。方法:将雄性SD大鼠随机分为假手术组、模型组、中药组(给予扶正化瘀降浊通络方),采用“5/6肾切除法”造模,动态观察一般情况,给药后4、8、12周通过检测免疫指标CD4^(^(+))、CD8^(^(+))细胞比率评估“正虚”情况,检测微炎症指标C反应蛋白(c reactive protein,CRP)、肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)、白介素-1β(Interleukin-1β,IL-1β)、白介素-6(Interleukin-6,IL-6)水平评估“邪实”情况。结果:模型组与假手术组比较,大鼠出现精神萎靡、毛发黄、目耳淡等本虚表现,伴舌紫黯、四肢肿胀等标实表现,全血中CD4^(^(+))降低、CD8^(^(+))升高,血清CRP、TNF-α、IL-1β、IL-6等微炎症因子表达增加(P<0.05,P<0.01);中药组与模型组比较,大鼠“本虚标实”的宏观表现有所改善,CD4^(^(+))升高、CD8^(^(+))降低,CRP、TNF-α、IL-1β、IL-6等微炎症因子表达量降低(P<0.05,P<0.01)。结论:5/6肾切除模型有“正虚邪实证”的宏观及微观表现,可作为“正虚邪实证”的动物证候模型,扶正化瘀降浊通络方可改善5/6肾切除大鼠“正虚邪实证”。

关键词：慢性肾衰竭动物模型正虚邪实证

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