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补骨脂酚通过抑制ERK1/2磷酸化并上调ABCA1表达减少巨噬细胞源性泡沫细胞形成

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中国动脉硬化杂志 2024年第9期32卷 763-770页

作者：王楊周琴怡王刚唐朝克南华大学衡阳医学院心血管疾病研究所湖南省动脉硬化学重点实验室湖南省动脉硬化性疾病国际科技合作创新基地南华大学衡阳医学院附属南华医院心内科心脏疾病多组学与人工智能湖南省重点实验室南华大学衡阳医学院附属第一医院心内科湖南省心肌损伤临床医学研究中心南华大学衡阳医学院附属第一医院心血管疾病研究所湖南省衡阳市421001

[目的]探讨补骨脂酚(BAK)对巨噬细胞源性泡沫细胞脂质蓄积的影响及机制。[方法]MTT筛选BAK对泡沫细胞药物毒性浓度;油红O染色、NBD胆固醇、Dil-ox-LDL检测泡沫细胞内脂质蓄积情况;使用RT-qPCR和Western blot检测mRNA和蛋白表达。[结果]... 详细信息

[目的]探讨补骨脂酚(BAK)对巨噬细胞源性泡沫细胞脂质蓄积的影响及机制。[方法]MTT筛选BAK对泡沫细胞药物毒性浓度;油红O染色、NBD胆固醇、Dil-ox-LDL检测泡沫细胞内脂质蓄积情况;使用RT-qPCR和Western blot检测mRNA和蛋白表达。[结果]BAK可以促进胆固醇流出并减少泡沫细胞内脂质蓄积。BAK可以上调三磷酸腺苷结合盒转运体A1(ABCA1)的mRNA和蛋白表达水平,同时可下调细胞外信号调节激酶1/2(ERK1/2)磷酸化水平。使用ERK1/2激动剂Ro 67-7476处理发现,与BAK处理组相比,加入Ro 67-7476处理后ABCA1蛋白表达下降。[结论]BAK通过抑制ERK1/2的磷酸化,上调ABCA1的表达并促进胆固醇的流出,减少泡沫细胞中的脂质蓄积,从而抑制泡沫细胞的形成。

关键词：补骨脂酚泡沫细胞三磷酸腺苷结合盒转运体A1 细胞外信号调节激酶1/2

Development and validation of an HPLC–MS/MS method to determine clopidogrel in human plasma

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Acta Pharmaceutica Sinica B 2016年第1期6卷 55-63页

作者： Gangyi Liu Chunxia Dong Weiwei Shen Xiaopei Lu Mengqi Zhang Yuzhou Gui qinyi zhou Chen Yu Central Laboratory Shanghai Clinical CenterChinese Academy of Sciences/Central LaboratoryShanghai Xuhui Central Hospital State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of Science

A quantitative method for clopidogrel using online-SPE tandem LC–MS/MS was developed and fully validated according to the well-established FDA *** method achieves adequate sensitivity for pharmacokinetic studies,with lower limit of quantifications(LLOQs)as low as 10 pg/m *** separations were performed on reversed phase columns Kromasil Eternity-2.5-C18-UHPLC for both *** electrospray ionization in multiple reaction monitoring(MRM)mode was employed for signal detection and a deuterated analogue(clopidogrel-d_4)was used as internal standard(IS).Adjustments in sample preparation,including introduction of an online-SPE system proved to be the most effective method to solve the analyte back-conversion in clinical *** clinical samples(two levels)were prepared and successfully used as real-sample quality control(QC)in the validation of back-conversion testing under different *** result showed that the real samples were stable in room temperature for 24 ***,precision,extraction recovery,matrix effect on spiked QC samples and stability tests on both spiked QCs and real sample QCs stored in different conditions met the acceptance *** online-SPE method was successfully applied to a bioequivalence study of75 mg single dose clopidogrel tablets in 48 healthy male subjects.

关键词： Clopidogrel Online-SPE LC–MS/MS Back-conversion Bioequivalence Deuterated analogue Real samples stability FDA guidelines

2013—2023年南京市流感样病例及流感病毒病原学特征分析

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首都公共卫生 2024年第2期18卷 81-86页

作者：胡洋田梦圆周沁易丁松宁吴小清南京市疾病预防控制中心江苏210003

目的了解2013—2023年南京市流感样病例(influenza-like illness, ILI)的流行病学特征及病原学变化趋势,为流行性感冒(以下简称流感)防控提供科学依据。方法通过中国疾病预防控制信息系统收集2013—2023年南京市ILI监测数据、病原学... 详细信息

目的了解2013—2023年南京市流感样病例(influenza-like illness, ILI)的流行病学特征及病原学变化趋势,为流行性感冒(以下简称流感)防控提供科学依据。方法通过中国疾病预防控制信息系统收集2013—2023年南京市ILI监测数据、病原学监测、暴发疫情数据并进行统计分析。结果 2013—2023年南京市哨点医院共报告1 919 187例ILI,ILI百分比(ILI%)为5.85%,总体呈先上升后下降趋势。2018—2019年ILI%最高(11.00%),2016—2017年最低(2.62%)。ILI以0~<5岁年龄组为主,占66.21%。共检测ILI样本45 074份,流感病毒阳性率为11.68%(5 265份),其中甲型H3N2最多(2 206份,占41.90%),其次为乙型Victoria系(1 224份,占23.25%)、甲型H1N1(1 201份,占22.81%)和乙型Yamagata系(599份,占11.38%)。不同监测年度阳性率比较,差异有统计学意义(χ2=1 282.83,P<0.001)。除2020—2021年没有流行高峰,其余9个年份均在冬春季出现了流行高峰,其中2014—2015、2015—2016、2017—2018、2021—2022和2022—2023年还出现了夏季流行高峰。2013—2023年南京市共报告ILI暴发疫情96起,主要发生在中小学校(含托幼机构),共94起(占97.92%)。结论南京市流感流行以冬春季为主,不同监测年度各流感亚型交替流行。0~<15岁儿童是流感防控重点人群,学校是重点防控场所。应持续加强和规范流感监测工作,密切关注南京市流感活动水平和趋势,及时进行预警和预测,科学精准防控流感疫情。

关键词：流行性感冒流感样病例病原学监测哨点监测

南京市输入的新型冠状病毒感染病例的流行特征分析

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医学动物防制 2024年第9期40卷 847-850页

作者：赵跃媛马涛周沁易严子康王恒学丁松宁南京市疾病预防控制中心急性传染病防制科江苏南京210003

目的分析南京市2020年1月—2022年7月报告输入的新型冠状病毒感染病例的流行特征,为疫情防控提供参考依据。方法通过中国疾病预防控制信息系统、流行病学调查报告获取感染者资料,对相关数据进行描述流行病学分析。结果2020年1月1日—202... 详细信息

目的分析南京市2020年1月—2022年7月报告输入的新型冠状病毒感染病例的流行特征,为疫情防控提供参考依据。方法通过中国疾病预防控制信息系统、流行病学调查报告获取感染者资料,对相关数据进行描述流行病学分析。结果2020年1月1日—2022年7月31日,南京市共报告输入的新型冠状病毒感染病例360例,63.89%(230/360)为无症状感染者。男女性别比为2.40∶1,发病年龄以20~<40岁为主。58.06%(209/360)的COVID-19病例来自意大利、俄罗斯、新加坡和尼日利亚。入境—首次核酸采样阳性的时间间隔中位数(median,M)(P_(25),P_(75))为2(0,6)d,33.06%(119/360)的COVID-19病例在入境当日采样即核酸阳性。从新型冠状病毒疫苗接种情况看,48.61%(175/360)输入的新型冠状病毒感染病例接种过新型冠状病毒疫苗,不同年龄组的接种情况差异有统计学意义(χ^(2)=9.593,P=0.044)。是否接种过新型冠状病毒疫苗在Ct值上差异无统计学意义[ORF1ab基因(硕世试剂:Z=-0.223,P=0.824;达安试剂:Z=-0.150,P=0.880);N基因(硕世试剂:Z=-1.024,P=0.306;达安试剂:Z=-0.580,P=0.562)]。结论南京市输入的新型冠状病毒感染病例以无症状为主,中青年男性居多。作为报告南京市输入的新型冠状病毒感染病例最多的禄口机场,外防输入仍是其疫情防控工作的重点。

关键词：新型冠状病毒感染输入病例流行特征分析

Sema3A secreted by sensory nerve induces bone formation under mechanical loads

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International Journal of Oral Science 2024年第1期16卷 62-72页

作者： Hongxiang Mei Zhengzheng Li qinyi Lv Xingjian Li Yumeng Wu Qingchen Feng Zhishen Jiang Yimei zhou Yule Zheng Ziqi Gao Jiawei zhou Chen Jiang Shishu Huang Juan Li State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&West China Hospital of Stomatology Sichuan UniversityChengduChina Department of Orthopedic Surgery and Orthopedic Research Institute West China HospitalSichuan UniversityChengduChina

Bone formation and deposition are initiated by sensory nerve infiltration in adaptive bone remodeling. Here, we focused on the role of Semaphorin 3A(Sema3A), expressed by sensory nerves, in mechanical loads-induced bone formation and nerve withdrawal using orthodontic tooth movement(OTM) model. Firstly, bone formation was activated after the 3rd day of OTM,coinciding with a decrease in sensory nerves and an increase in pain threshold. Sema3A, rather than nerve growth factor(NGF),highly expressed in both trigeminal ganglion and the axons of periodontal ligament following the 3rd day of OTM. Moreover, in vitro mechanical loads upregulated Sema3A in neurons instead of in human periodontal ligament cells(hPDLCs) within 24 ***, exogenous Sema3A restored the suppressed alveolar bone formation and the osteogenic differentiation of hPDLCs induced by mechanical overload. Mechanistically, Sema3A prevented overstretching of F-actin induced by mechanical overload through ROCK2 pathway, maintaining mitochondrial dynamics as mitochondrial fusion. Therefore, Sema3A exhibits dual therapeutic effects in mechanical loads-induced bone formation, both as a pain-sensitive analgesic and a positive regulator for bone formation.

关键词： Sema3A formation overload

MOF-based magnetic microrobot swarms for pH-responsive targeted drug delivery

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Science China Chemistry 2024年第4期67卷 1216-1223页

作者： qinyi Cao Yifan Zhang Yanping Tang Changjin Wu Jizhuang Wang Dan Li College of Chemistry and Materials Science Jinan UniversityGuangzhou 510632China Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications Jinan UniversityGuangzhou 510632China Guangdong Provincial Key Laboratory of Spine and Spinal Cord Reconstruction The Fifth Affiliated Hospital(Heyuan Shenhe People’s Hospital)Jinan UniversityHeyuan 517000China JNU-HKUST Joint Laboratory for Neuroscience and Innovative Drug Research College of PharmacyJinan UniversityGuangzhou 510632China Department of Chemistry The University of Hong KongHong KongChina

Metal-organic frameworks(MOFs)hold significant potential as vehicles for drug delivery due to their expansive specific surface area,biocompatibility,and versatile ***,magnetically actuated micro/nano-robots(MNRs)offer distinct advantages,such as untethered and precise *** fusion of these technologies presents a promising avenue for achieving non-invasive targeted drug ***,we report a MOF-based magnetic microrobot swarm(MMRS)for targeted *** approach overcomes limitations associated with a single MNR,including limited drug loading and the risk of loss during *** select Zeolitic Imidazolate Framework-8(ZIF-8)as the drug vehicle for its superior loading potential and p H-sensitive *** design incorporates magnetic responsive components into the one-pot synthesis of Fe@ZIF-8,enabling collective behaviors under *** the yaw angle of alternating magnetic fields and nanoparticles'amount,the MMRSs with controllable size achieve instantaneous transformation among different configurations,including vortex-like swarms,chain-like swarms,and elliptical swarms,facilitating adaptation to environmental *** to the subcutaneous T24 tumor site,the MMRSs with encapsulated doxorubicin(DOX)automatically degrade and release the drug,leading to a dramatic reduction of the tumor in *** investigation signifies a significant advancement in the integration of biodegradable MOFs into microrobot swarms,ushering in new avenues for accurate and non-invasive targeted drug delivery.

关键词： magnetic microrobot swarm MOF nanoparticles magnetic propulsion pH-responsive drug release targeted drug delivery

Tailoring the austenite characteristics via dual nanoparticles to synergistically optimize the strength-ductility in cold rolled medium Mn steel

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Journal of Materials Science & Technology 2024年第2期169卷 158-171页

作者： Qizhe Ye Haokai Dong qinyi Guo Yishuang Yu Lijie Qiao Yu Yan Beijing Advanced Innovation Center for Materials Genome Engineering Corrosion and Protection CenterInstitute for Advanced Materials and TechnologyUniversity of Science and Technology BeijingBeijing 100083China School of Mechanical and Automotive Engineering South China University of TechnologyGuangzhou 510641China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200240China

In this work,we proposed a novel Cu/θdual nanoparticles strategy to tailor the austenite characteris-tics of a medium Mn steel via a tempering-annealing process to optimize the mechanical *** explored the effects of Cu-rich particles and cementite precipitated in the tempering process on the austenite reversion during the subsequent annealing *** experiments and numerical simula-tions verified that the austenite inherited from cementite had a finer size and a higher Mn enrichment compared with the austenite originating from the tempered martensite *** addition,quantitative evaluations revealed that the pinning effect exerted by the Cu-rich particles could significantly hinder theα/γinterface migration and the recrystallized grain growth,thereby further refining the final *** contributions from the effects of dual nanoprecipitates on the austenite reversion,the heterogeneous austenite grains inherited from varying nucleation sites ensured the sustained and gradual deformation-induced martensite and twinning ***,the Cu-added steels subjected to a tempering-annealing process achieved synergetic enhancement of the tensile strength from 1055 MPa to 1250 MPa and elongation from 33%to 45%.This strategy may provide new guidance for the development and alloy design of high-performance medium Mn steels.

关键词： Dual nanoparticles Medium Mn steel Tempering-annealing process Austenite reversion Mechanical properties

Research on Variation of Bamboo Density

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Chinese Forestry Science and Technology 2009年第1期8卷 24-28页

作者： LI Xiazhen REN Haiqing zhou qinyi LOU Wanli GUO Yan Research Institute of Wood Industry Chinese Academy of ForestryBeijing 100091P.R.China Chinese Academy of Forestry Beijing 100091P.R.China

Bamboo as an important biological resource has become an alternative to wood gradually,but the variation in mechanical properties leads to difficulty in its use to some *** deep understanding of the nature in bamboo is needed to satisfy the utilization *** we have known,the density is one of mechanical properties of ***,this paper studied the variation in bamboo density with the weighing *** results showed that the density decreases gradually from the outer to the inn...

关键词： Moso bamboo(Phyllostachys heterocycla) density variability

Promising real-time fruit and vegetable quality detection technologies applicable to manipulator picking process

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International Journal of Agricultural and Biological Engineering 2024年第2期17卷 14-26页

作者： Jintao Feng qinyi Yang Hao Tian Zhipeng Wang Shijie Tian Huirong Xu College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310058China Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province Hangzhou 310058China

In recent years,worldwide research on fruit and vegetable quality detection technology includes machine vision,spectroscopy,acoustic vibration,tactile sensors,*** technologies have also been gradually applied to fruit and vegetable grading and sorting lines in recent years,greatly improving the income of *** have been numerous reviews of these *** of the published research on fruit and vegetable quality detection technology is still carried out in the *** emphases have been on quality feature extraction,model establishment and experimental *** successful application in the fruit and vegetable sorting production line proves that these studies have high application potential and value,and we look forward to the performance of these sensing technologies in the fruit and vegetable picking ***,in this paper,based on the future highly automated fruit and vegetable picking mode,we will focus on three kinds of fruit and vegetable quality detection technologies including machine vision,tactile sensor and spectroscopy,to provide some reference for future *** there are currently limited cases of detecting quality during the fruit and vegetable picking,experiments performed on prototypes of manipulator,or devices such as Nanocilia sensors,portable spectrometers,etc.,which are compact and convenient to mount on manipulator will be *** tables and mosaics showing the performance of the three technologies in the detection of fruit and vegetable quality over the past five years have been *** performance of each sensing technology was relatively satisfactory in the laboratory in ***,in the picking scenario,there are still many challenges to be *** from industrial environments,agricultural scenarios are complex and *** and vulnerable agricultural products pose another *** development of portable devices and nanomaterials have become important

关键词： fruit and vegetable quality detection machine vision spectroscopy tactile sensors picking scenario