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Journal of Pharmaceutical Analysis 2024年第1期14卷 140-148页

作者： Jingxian Zhang qinsheng chen Lianglong Zhang Biru Shi Men Yu Qingxia Huang Huiru Tang State Key Laboratory of Genetic Engineering School of Life SciencesHuman Phenome InstituteZhangjiang Fudan International Innovation CenterMetabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular PhenomicsZhongshan HospitalFudan UniversityShanghai200032China Wuhan Laboratory for Shanghai Metabolome Institute(SMI)Ltd. Wuhan430000China

Acylcarnitines are metabolic intermediates of fatty acids and branched-chain amino acids having vital biofunctions and pathophysiological significances. Here, we developed a high-throughput method for quantifying hundreds of acylcarnitines in one run using ultrahigh performance liquid chromatography and tandem mass spectrometry (UPLC-MS/MS). This enabled simultaneous quantification of 1136 acylcarnitines (C0–C26) within 10-min with good sensitivity (limit of detection 0.992), accuracy (relative error 0.998) for predicting retention time (tR) of acylcarnitines with no standards and built a database of their multiple reaction monitoring parameters (tR, ion-pairs, and collision energy). Furthermore, we quantified 514 acylcarnitines in human plasma and urine, mouse kidney, liver, heart, lung, and muscle. This provides a rapid method for quantifying acylcarnitines in multiple biological matrices.

关键词： Acylcarnitine UPLC-MS/MS Quantitative structure-retention relationship Molecular phenotype

Rapid quantification of 50 fatty acids in small amounts of biological samples for population molecular phenotyping

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Biophysics Reports 2023年第6期9卷 299-308页

作者： Pinghui Liu qinsheng chen Lianglong Zhang chengcheng Ren Biru Shi Jingxian Zhang Shuaiyao Wang Ziliang chen Qi Wang Hui Xie Qingxia Huang Huiru Tang State Key Laboratory of Genetic Engineering School of Life Sciences Human Phenome Institute Zhangjiang Fudan International Innovation Center Metabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular Phenomics Zhongshan Hospital Fudan University Wuhan Laboratory for Shanghai Metabolome Institute (SMI) Ltd

Efficient quantification of fatty-acid(FA) composition(fatty-acidome) in biological samples is crucial for understanding physiology and pathophysiology in large population cohorts. Here, we report a rapid GC-FID/MS method for simultaneous quantification of all FAs in numerous biological matrices. Within eight minutes, this method enabled simultaneous quantification of 50 FAs as fatty-acid methyl esters(FAMEs) in femtomole levels following the efficient transformation of FAs in all lipids including FFAs,cholesterol-esters, glycerides, phospholipids and sphingolipids. The method showed satisfactory interday and intra-day precision, stability and linearity(R2 > 0.994) within a concentration range of 2–3 orders of magnitude. FAs were then quantified in typical multiple biological matrices including human biofluids(urine, plasma) and cells, animal intestinal content and tissue samples. We also established a quantitative structure-retention relationship(QSRR) for analytes to accurately predict their retention time and aid their reliable identification. We further developed a novel no-additive retention index(NARI) with endogenous FAMEs reducing inter-batch variations to 15 seconds; such NARI performed better than the alkanes-based classical RI, making meta-analysis possible for data obtained from different batches and platforms. Collectively, this provides an inexpensive high-throughput analytical system for quantitative phenotyping of all FAs in 8-minutes multiple biological matrices in large cohort studies of pathophysiological effects.

关键词： Fatty-acidomics High-throughput quantification Structure-retention relationship No-additive retention index

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国内环氧浇注干式变压器的现状及其发展

变压器 2000年第8期37卷 8-10页

作者：陈琴生中国电机工程学会城市供电专委会上海200081

介绍了国内环氧浇注干式变压器的结构形式及其引进的技术。

关键词：干式变压器环氧浇注填料局部放电电力变压器

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双光纤布拉格光栅电流传感器

应用激光 2007年第4期27卷 338-341页

作者：陈钦生杨淑连山东理工大学物理与光电信息技术学院山东淄博255049 山东理工大学电气与电子工程学院山东淄博255049

两电流产生的电磁力使等腰三角形悬臂梁变形,从而导致安装在悬臂梁两边的光纤布拉格光栅的布拉格波长漂移。通过检测两个布拉格光栅的波长漂移差,得到被测电流。双光纤布拉格光栅通过补偿温度效应,解决了光纤布拉格光栅传感器的交叉敏... 详细信息

两电流产生的电磁力使等腰三角形悬臂梁变形,从而导致安装在悬臂梁两边的光纤布拉格光栅的布拉格波长漂移。通过检测两个布拉格光栅的波长漂移差,得到被测电流。双光纤布拉格光栅通过补偿温度效应,解决了光纤布拉格光栅传感器的交叉敏感问题。垂直放置的等腰三角形悬臂梁,确保光纤光栅在传感过程中不出现啁啾现象,又避免了自身重量和导线重量对测量结果的影响,从而减少了测量误差。该系统传感灵敏度为0.097nm/A,与理论值的相对误差为3.38%,结果表明该传感器结构是可行的。

关键词：光纤传感器电流传感器电流测量光纤布拉格光栅

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对干式变压器的展望

供用电 2007年第1期24卷 52-53页

作者：陈琴生厦门ABB电器控制设备有限公司福建厦门361004

对国内干式变压器近20年来的发展情况作一简要的回顾,分析了目前产品的技术经济参数和产品的结构,结合当前国家对产品的节能和环保的要求提出了一些看法和建议。

关键词：干式变压器环氧树脂节能环保

显露喉返神经和甲状旁腺在甲状腺癌根治术中的临床价值探讨

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临床医学工程 2019年第9期26卷 1229-1230页

作者：陈海德陈龙林陈钦盛广东省汕头市潮阳区大峰医院

目的探讨显露喉返神经和甲状旁腺在甲状腺癌根治术中的临床价值。方法选择我院2017年3月至2019年2月期间收治的甲状腺癌患者65例,根据术中是否显露喉返神经和甲状旁腺分为实验组(n=35)和对照组(n=30),对比两组患者的术中出血量、术后引... 详细信息

目的探讨显露喉返神经和甲状旁腺在甲状腺癌根治术中的临床价值。方法选择我院2017年3月至2019年2月期间收治的甲状腺癌患者65例,根据术中是否显露喉返神经和甲状旁腺分为实验组(n=35)和对照组(n=30),对比两组患者的术中出血量、术后引流量、住院时间及喉返神经和甲状旁腺功能损伤情况。结果实验组的术中出血量、术后引流量及住院时间明显优于对照组,差异有统计学意义(P<0.05)。实验组的喉返神经和甲状旁腺功能损伤率分别为5.7%、2.9%,明显低于对照组的23.3%、20.0%,差异有统计学意义(P<0.05)。结论甲状腺癌根治术中显露喉返神经和甲状旁腺能够促进患者更快恢复,喉返神经和甲状旁腺功能损伤率较低,具有一定的临床应用价值。

关键词：喉返神经甲状旁腺甲状腺癌根治术临床价值

拟穴青蟹对急性低盐胁迫的神经内分泌-免疫响应

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海洋湖沼通报 2023年第4期45卷 63-72页

作者：张岩吴翔宇陈钦胜唐贤明赵群李二超海南大学海洋学院海南海口570228 海南省热带海水养殖技术重点实验室海南省海洋与渔业科学院海南海口571126

本文以拟穴青蟹(Scyllaparamamosain)原生盐度15为对照,以低盐度5为处理,在实验周期192h内,初步研究了急性低盐胁迫对甲壳动物神经内分泌-免疫调控机制的影响。结果发现:低盐处理组中的促肾上腺皮质激素释放激素和促肾上腺皮质激素浓度... 详细信息

本文以拟穴青蟹(Scyllaparamamosain)原生盐度15为对照,以低盐度5为处理,在实验周期192h内,初步研究了急性低盐胁迫对甲壳动物神经内分泌-免疫调控机制的影响。结果发现:低盐处理组中的促肾上腺皮质激素释放激素和促肾上腺皮质激素浓度在12h内显著升高(P<0.05);多巴胺、去甲肾上腺素及5-羟色胺浓度在0~48h内呈峰值的变化,在12h达到最大值(P<0.05),在48h后基本恢复至对照组水平。低盐胁迫下,青蟹血细胞数量在实验时间内呈峰值变化,至12h达到最小值并保持稳定,与对照组呈显著差异(P<0.05);血细胞酚氧化酶原激活因子和酚氧化酶原基因表达及其活性均在0~48h内呈峰值变化,12h至最小值(P<0.05);酚氧化酶活性在12h内显著升高(P<0.05);随后以上指标均在48h基本恢复至对照组水平;吞噬率在12h内显著下降(P<0.05),48h后恢复至对照组水平;类甲壳肽基因表达量及抗菌活性均在0~96h内呈峰值变化,至12h达到最小值(P<0.05),96h恢复至对照组水平;C-型凝集素基因表达量和凝集活性在12h内显著下降(P<0.05),48h时逐渐恢复至对照组水平。结果提示,低盐胁迫下,拟穴青蟹通过激素、生物胺等神经内分泌因子调控机体免疫响应,造成短时间内的免疫抑制,调控路径为CRH➝ACTH➝生物胺➝血细胞免疫响应。

关键词：拟穴青蟹急性低盐胁迫激素生物胺免疫调控机制

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簇发振荡能量采集动力学理论研究进展

动力学与控制学报 2024年第8期22卷 1-12页

作者：姜文安卞明礼陈立群毕勤胜江苏大学土木工程与力学学院镇江212013 上海大学力学与工程科学学院上海200072

将自然环境中的振动能,通过能量采集装置转化为电能,为低功耗的电子器件提供清洁可持续的能源,对推动无线传感电子技术的发展具有重要意义.当环境的振动频率远低于采集装置的固有频率时,传统振动能量采集技术的采集效率将会明显降低,无... 详细信息

将自然环境中的振动能,通过能量采集装置转化为电能,为低功耗的电子器件提供清洁可持续的能源,对推动无线传感电子技术的发展具有重要意义.当环境的振动频率远低于采集装置的固有频率时,传统振动能量采集技术的采集效率将会明显降低,无法匹配低频的环境激励,而基于低频激励的簇发振荡通常表现为激发态的大幅振荡与沉寂态的微幅振荡的组合,具有大幅运动的高能轨道,在低频慢变激励的环境下依然保持较为良好的采集性能.因此,开展适应低频激励环境的能量采集技术的研究是很有必要.本文系统地概述了簇发振荡能量采集器的机电耦合能量转换装置、簇发振荡能量采集的动力学机理、簇发振荡能量采集的效率三个方面的研究进展.最后,给出了开展簇发振荡能量采集技术研究的一些建议.

关键词：簇发振荡能量采集快慢分析分岔理论

Improving energy harvesting by internal resonance in a spring-pendulum system

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Acta Mechanica Sinica 2020年第3期36卷 618-623页

作者： Wenan Jiang Xiujing Han Liqun chen qinsheng Bi Faculty of Civil Engineering and Mechanics Jiangsu UniversityZhenjiang 212013China School of Science Harbin Institute of TechnologyShenzhen 518055China Department of Mechanics Shanghai UniversityShanghai 200072China

This paper proposes a two-to-one internal resonance to widen the bandwidth of vibratory energy *** describe the improved characteristic,an electromagnetic spring-pendulum harvester is *** analytical solutions of the electromechanical coupled system are carried out by introducing the method of multiple scales,and the frequency response relationships of the displacement and the current are *** character of broadband harvesting performance is examined,the two peaks and double jump phenomena for variation of design parameters were *** effect of key control parameters on the harvesters bandwidth is considered,and the nonlinear behaviors of the harvester are validated via numerical results.

关键词： Energy harvesting Internal resonance Broadband Nonlinear modal interactions

Relaxation oscillations induced by an order gap between exciting frequency and natural frequency

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Science China(Technological Sciences) 2017年第2期60卷 289-298页

作者： chen XiaoKe LI ShaoLong ZHANG ZhengDi BI qinsheng Faculty of Civil Engineering and Mechanics Jiangsu University Zhenjiang 212013 China Faculty of Science Jiangsu University Zhenjiang 212013 China

The main purpose of the paper is to display the relaxation oscillations, known as the bursting phenomena, for the coupled oscillators with periodic excitation with an order gap between the exciting frequency and the natural frequency. For the case when the exciting frequency is much smaller than the natural frequency, different types of bursting oscillations such as fold/fold, Hopf/Hopf bursting oscillations can be observed. By regarding the whole exciting term as a slow-varying parameter on the fact that the exciting term changes on a much smaller time scale, bifurcations sets of the generalized autonomous system is derived, which divide the parameter space into several regions associated with different types of dynamical behaviors. Two cases with typical bifurcation patterns are focused on as examples to explore the dynamical evolution with the variation of the amplitude of the external excitation. Bursting oscillations which alternate between quiescent states (QSs) and repetitive spiking states (SPs) can be obtained, the mechanism of which is presented by introducing the transformed phase portraits overlapping with the bifurcation diagrams of the generalized autonomous system. It is found that not only the forms of QSs and SPs, but also the bifurcations at the transition points between QSs and SPs, may influence the structures of bursting attractors. Furthermore, the amplitudes and the frequencies related to SPs may depend on the bifurcation patterns from the quiescent sates.

关键词： coupled oscillators periodic excitation bursting oscillations bifurcation mechanism