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文献报告会在中医院校基础医学学术型研究生培养中的应用与评价

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中国中医药现代远程教育 2024年第5期22卷 190-193页

作者：成细华雷晓明杨冬梅李亚楠庹勤慧湖南中医药大学医学院湖南长沙410208

目的探讨文献报告会在中医院校基础医学学术型研究生培养中的应用效果和满意度评价。方法选取湖南中医药大学医学院2020级、2021级基础医学学术型在读研究生29名作为研究对象,根据研究生对文献报告会满意度、报告会前后科研各项能力... 详细信息

目的探讨文献报告会在中医院校基础医学学术型研究生培养中的应用效果和满意度评价。方法选取湖南中医药大学医学院2020级、2021级基础医学学术型在读研究生29名作为研究对象,根据研究生对文献报告会满意度、报告会前后科研各项能力自评、文献报告会对研究生综合能力影响评价及师生交流情况设计问卷调查。结果参与文献报告会后,86.21%(25/29)的研究生表示满意文献报告会。调查对象自评其文献检索能力、文献阅读分析能力、文献撰写能力、制作学术PPT能力和文献汇报能力均有显著提升(P<0.05)。89.66%(26/29)的调查对象认同参加文献报告会有助于开拓学术视野,有助于提高创新意识和创新能力;79.31%(23/29)的调查对认同其巩固了基础理论和专业知识;72.41%(21/29)认同其增强了自主学习能力及主动参加线上线下的学术专题讲座意愿。文献报告会也增加了导师与研究生的交流方式和见面频率。结论文献报告会促进了导师与研究生之间的交流,对研究生科研综合能力的培养有积极作用。

关键词：文献报告会基础医学学术型研究生中医教育

Effect of Daxx on apoptosis of RAW264.7 cells induced by cholesterol:model of β-cyclodextrin

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Effect of Daxx on apoptosis of RAW264.7 cells induced by cho...

第11届全国脂质与脂蛋白学术会议

作者： Shuhua Cui qinhui tuo DuanFang Liao Learning Key Laboratory for Pharmacoproteomics School of Life Science and TechnologyUniversity of South China Department of Traditional Chinese Diagnotics School of PharmacyHunan University of Chinese Medicine

Objective:The aim of the present study was to determine the effect of Daxx on apoptosis of RAW264.7 cells induced by ***. Methods:The eukaryotic vector of Daxx was constructed to be stably transfected into RAW264.7 *** detected the expression of Daxx in cells transfected with reorganized vector using RT-PCR and western blotting to decide whether the eukaryotic vector is successfully ***,the recombinant

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Daxx and the functional domain regulate cholesterol contents in HepG2 cells

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Daxx and the functional domain regulate cholesterol contents...

第11届全国脂质与脂蛋白学术会议

作者： Juan Wen qinhui tuo Duan Fang Liao Learning Key Laboratory for Pharmacoproteomics School of Life Science and TechnologyUniversity of South China Department of Traditional Chinese Diagnotics School of PharmacyHunan University of Chinese Medicine

Object:To observe the effects and mechanisms of the functional domain and the full-length of Daxx on the celluar *** to investigate a new way for the prevention and treatment of hyperlipidemia and related acute coronary events. Methods:The Fas death domain-associated protein(Daxx) functional domain mutant and full-length Daxx eukaryotic expression vectors were stably transfected into HepG *** to establish stable cell lines successfully was identified by Western *** interaction of Daxx and AR was observed by ***

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Daxx as a novel inhibitor of vascular smooth muscle cell proliferation and intima hyperplasia

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Daxx as a novel inhibitor of vascular smooth muscle cell pro...

第十一届海峡两岸心血管科学研讨会、2017年全国心脑血管药理学术会议、中国卒中学会第一次新药研讨会

作者： Sun Siyu Chen Jie Chen Jianxiong tuo qinhui Hunan University of Chinese Medicine University of South China University of Mississippi Medical Center

Abnormal proliferation of vascular smooth muscles(VSMCs) is one of the main causes of intima hyperplasia after percutaneous coronary intervention(PCI).Fas death domain-associate protein(Daxx) have various biological functions,such as regulate transcription,cell apoptosis and proliferation,anti-virus ***,the role of Daxx in VSMCs is *** aimed to tested the effect of Daxx on VSMCs proliferation in vitro and intima hyperplasia after vascular injury in ***,we observated that the expression of Daxx is decreased on time-dependent in proliferative VSMCs induced by platelet-derived growth factor(PDGF).Then,by established stable cell lines of pCDNA3.1-Daxx or shRNA-Daxx,Daxx suppressed PDGF-induce VSMCs proliferation,which was associated with inhibition of S phase of cell ***,Daxx increased the levels of PTEN mRNA and protein assayed by RT-PCR and Western *** is important for the VSMC ***,52 male rats were builted the restonsis model of balloon-injured carotid artery by using liposome topical transfection DAXX plasmid;Compared with surgery group,the intimal/medical area ratio was singificantly lower in transfected *** we conclused that Daxx is possiably a novel inhibitor of intima hyperplasia by regulateing VSMC proliferation through PTEN/Akt signaling pathway.

关键词： Daxx as a novel inhibitor of vascular smooth muscle cell proliferation and intima hyperplasia

Advances in the study of the biological function of Daxx

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Advances in the study of the biological function of Daxx

第11届全国脂质与脂蛋白学术会议

作者： Jinhong Liu GuoZuo Xiong DuanFang Liao qinhui tuo Learning Key Laboratory for Pharmacoproteomics School of Life Science and TechnologyUniversity of South China Department of General Surgery The Second Affiliated Hospital in University of South China Department of Traditional Chinese Diagnotics School of PharmacyHunan University of Chinese Medicine

Death domain-associated protein(Daxx) was originally identified as a protein that specifically binds to the death domain of the transmembrane death receptor FAS(also called CD95) in the cytoplasm and potentiates FAS-induced *** of Daxx enhances Fas-mediated apoptosis and activates the Jun N-terminal kinase(JNK) *** was found in the nuclears where it localizes to promyelocytic leukaemia(PML) oncogenic domains(PODs).As a highly conserved nuclear protein,Daxx plays a important role in proapoptosis,antiapoptosis, transcriptional *** article reviews the latest advances in the study on biological function of Daxx.

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Effect of overexpressed Daxx on apoptosis of RAW264.7 cells induced by Chol:MβCD

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Effect of overexpressed Daxx on apoptosis of RAW264.7 cells ...

脂质组学与脂蛋白的新功能——第十二届全国脂质与脂蛋白学术会议

作者： qinhui tuo Shuhua Cui Guozuo Xiong Tianping Li Juan Wen duanfang Liao Hunan University of Chinese Medicine Key Laboratory for Pharmacoproteomics School of Life Science and TechnologyUniversity of South China Department of general surgery the second affiliated hospital in University of South China

To explore whether Daxx mediates Chol:MβCD induced cholesterol accumulation and apopotosis in *** eukaryotic vector of Daxx was constructed to be stably transfected into RAW264.7 cells,the mRNA and protein expressions of Daxx was quantified by RT-PCR and western blotting,*** lipiddroplets and lipid levels were assayed by oil red Q staining and enzymefluorescent *** growth inhibition

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动脉粥样硬化病证结合动物模型研究进展

中国比较医学杂志 2022年第1期32卷 147-150页

作者：全文娟廖端芳庹勤慧湘产大宗药材品质评价湖南省重点实验室湖南中医药大学药学院长沙410208 湖南中医药大学医学院长沙410208

动脉粥样硬化是多种急慢性心脑血管疾病的共同发病机理,受感人群广泛。近年来,祖国医学在防治动脉粥样硬化上发挥重要作用,深入探究中医药治疗动脉粥样硬化的药理机制有利于指导临床用药及新药开发。因此,如何精准建立动脉粥样硬化的病... 详细信息

动脉粥样硬化是多种急慢性心脑血管疾病的共同发病机理,受感人群广泛。近年来,祖国医学在防治动脉粥样硬化上发挥重要作用,深入探究中医药治疗动脉粥样硬化的药理机制有利于指导临床用药及新药开发。因此,如何精准建立动脉粥样硬化的病证结合动物模型亟待解决。本文就动脉粥样硬化病证结合动物模型的研究进展进行综述,以期为中医实验研究动物模型的制备提供依据。

关键词：动脉粥样硬化病证结合动物模型中医药综述

Living fossils unearthed by blasting human chromosomes with Neanderthal mtDNA

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Digital Chinese Medicine 2022年第3期5卷 236-241页

作者： ZHANG Jia ZHOU Cuilan XIAO Li tuo qinhui PENG Cuiying GUO Zifen LIAO Duanfang LI Kai National Engineering Research Center of Personalized Diagnostic and Therapeutic Technology Hunan University of Chinese MedicineChangshaHunan 410208China SNP Institute School of Life ScienceUniversity of South ChinaHengyangHunan 421001China Division of Stem Cell Regulation and Application Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan ProvinceHunan University of Chinese MedicineChangshaHunan 410208China

The successful retrieval of ancient mitochondrial DNA(mtDNA)from Neanderthals provides powerful experimental evidence that clarifies the arguments between the out-of-Africa and multiregional models of ***,the lack of nuclear DNA from Neanderthal fossils and mtDNA of early modern human fossils dating back to approximately the same time in the Pleistocene constitutes a limitation that may compromise the significance of mtDNA phylogenetic *** this report,we introduce a mitochromic analysis using Neanderthal mtDNA as a foreign transgene and humans as a naturally occurring transgenic *** Neanderthal mtDNA retrievable nuclear fragments were identified by blasting human genome data with Neanderthal *** of the 40 fragments exhibited higher correlation with Neanderthal mtDNA than those with modern human ***,these five nuclear fragments harbor Neanderthal mtDNA-unique *** on the 98%+identity between Neanderthal and modern human mtDNA when compared by groups,we suggest that some of the modern human nuclear fragments retrieved using Neanderthal mtDNA may aid in decoding Neanderthal genetic information,and also may simultaneously demonstrate a close genetic evolutionary relationship between modern humans and Neanderthals.

关键词： Neanderthal Mitochromics Human evolution Mitochondrial DNA(mtDNA)

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PTEN与动脉粥样硬化

临床与病理杂志 2023年第4期43卷 836-841页

作者：陈煜(综述) 庹勤慧(审校) 湖南中医药大学医学院血管生物学与转化医学湖南省重点实验室长沙410208

人第10号染色体缺失的磷酸酶及张力蛋白同源(phosphatase and tensin homolog deleted on chromosome ten,PTEN)基因作为一种抑癌基因,在调控肿瘤方面的作用广为人知。近年来研究发现,PTEN除了在肿瘤防治上发挥作用外,还能通过抑制炎症... 详细信息

人第10号染色体缺失的磷酸酶及张力蛋白同源(phosphatase and tensin homolog deleted on chromosome ten,PTEN)基因作为一种抑癌基因,在调控肿瘤方面的作用广为人知。近年来研究发现,PTEN除了在肿瘤防治上发挥作用外,还能通过抑制炎症、氧化应激的发展来减缓动脉粥样硬化(atherosclerosis,AS)的进程。同时,PTEN蛋白能够抑制AS中血管平滑肌细胞(vascular smooth muscle cells,VSMCs)的异常增殖,以及调控巨噬细胞极化的方向、改变巨噬细胞表面清道夫受体的类型来抗AS。而PTEN对于血管内皮细胞(vascular endothelial cells,VECs)的调控则具有双重性。文章对PTEN的结构、功能与发挥的调控作用做一简要综述,以期为AS防治的基础研究提供新的思路和靶点。

关键词：动脉粥样硬化平滑肌细胞内皮细胞氧化应激炎症染色体缺失的磷酸酶及张力蛋白同源