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Nanobodies against African swine fever virus p72 and CD2v proteins as reagents for developing two cELISAs to detect viral antibodies

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Virologica Sinica 2024年第3期39卷 478-489页

作者： Jiahong Zhu qingyuan liu liuya Li Runyu Zhang Yueting Chang Jiakai Zhao Siyu liu Xinyu Zhao Xu Chen Yani Sun Qin Zhao Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F UniversityYangling Observing and Experimental Station of National Data Center of Animal HealthMinistry of AgricultureYangling712100China

African swine fever virus(ASFV)poses a significant threat to the global swine ***,there are no effective vaccines or treatments available to combat ASFV infection in *** primary means of controlling the spread of the disease is through rapid detection and subsequent elimination of infected ***,a lower virulent ASFV isolate with a deleted EP402R gene(CD2v-deleted)has been reported in China,which further complicates the control of ASFV infection in pig ***,an EP402R-deleted ASFV variant has been developed as a potential live attenuated vaccine candidate ***,it is crucial to develop detection methods that can distinguish wild-type and EP402R-deleted ASFV *** this study,two recombinant ASFV-p72 and-CD2v proteins were expressed using a prokaryotic system and used to immunize Bactrian ***,eight nanobodies against ASFV-p72 and ten nanobodies against ASFV-CD2v were *** the production of these nanobodies with horse radish peroxidase(HRP)fusion proteins,the ASFV-p72-Nb2-HRP and ASFV-CD2v-Nb22-HRP fusions were selected for the development of two competitive ELISAs(cELISAs)to detect anti-ASFV *** two cELISAs exhibited high sensitivity,good specificity,repeatability,and *** coincidence rate between the two cELISAs and commercial ELISA kits was 98.6%and 97.6%,***,the two cELISA for detecting antibodies against ASFV demonstrated ease of operation,a low cost,and a simple production *** two cELISAs could determine whether pigs were infected with wild-type or CD2v-deleted ASFV,and could play an important role in monitoring ASFV infections in pig farms.

关键词： African swine fever virus(ASFV) ASFV-p72 ASFV-CD2v Nanobody-HRP Competitive ELISA

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Towards Subspace Identification with Time Delays via Parallel Knowledge-Enhanced Bayesian Optimization

Towards Subspace Identification with Time Delays via Paralle...

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第35届中国过程控制会议

作者： qingyuan liu Chao Shang Dexian Huang Department of Automation Tsinghua University

For decades, subspace identification method(SIM) has been widely adopted for modelling multiple-input multipleoutput(MIMO) processes. However, in face of processes with large time delays, it is necessary to use a high-order state-space model to capture the time-delay behavior, and thus the ordinary SIMs suffer from unsatisfactory modeling accuracy. To overcome this limitation, a viable solution is to consider time delays in state-space model. However, the subspace identification with time delays receives little attention and still remains a challenge. In this work, by exploiting advanced numerical optimization techniques, an efficient method for subspace identification with time delays is proposed, implemented with a novel parallel knowledge-enhanced Bayesian optimization(KeBO) scheme, where a parallel BO structure is designed to address the constraint in optimization problem, and a tailored acquisition function(AF) that enables the exploitation of process knowledge about time delays is adopted. The advantages of the proposed method in the identification of MIMO processes with large time delays are evidenced with numerical case studies.

关键词： Subspace identification Time-delayed systems Bayesian optimization

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Two nanobody‑based immunoassays to differentiate antibodies against genotype 1 and 2 porcine reproductive and respiratory syndrome virus

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Animal Diseases 2024年第2期4卷 98-114页

作者： Xu Chen Yueting Chang Lu Zhang Xinyu Zhao Zhihan Li Zhijie Zhang Pinpin Ji qingyuan liu Jiakai Zhao Jiahong Zhu Baoyuan liu Xinjie Wang Yani Sun Qin Zhao Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F UniversityYanglingShaanxi 712100China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518100China

Porcine reproductive and respiratory syndrome virus(PRRSV)infection causes significant economic loss to the global pig *** 1 and 2 PRRSV(PRRSV-1 and-2)infections have been reported in China,Europe and *** accurate prevention,nanobodies were first used as diagnostic reagents for PRRSV *** this study three nanobodies targeting both PRRSV-1 and-2,two targeting PRRSV-1 and three targeting PRRSV-2,were screened and *** develop two competitive ELISAs(cELISAs),the g1-2-PRRSV-Nb3-HRP nanobody was chosen for the g1-2-cELISA,to detect common antibodies against PRRSV-1 and-2,and the g1-PRRSV-Nb136-HRP nanobody was chosen for the g1-cELISA,to detect anti-PRRSV-1 *** two cELISAs were developed using PRRSV-1-N protein as coating antigen,and the amounts for both were 100 ng/*** optimized dilution of testing pig sera was 1:20,the optimized reaction times were 30 min,and the colorimetric reaction times were 15 ***,the cut-off values of the g1-2-cELISA and g1-cELISA were 26.6%and 35.6%,*** of them have high sensitivity,strong specificity,good repeatability,and *** addition,for the 1534 clinical pig sera,an agreement rate of 99.02%(Kappa values=0.97)was determined between the g1-2-cELISA and the commercial IDEXX ELISA *** the g1-cELSIA,it can specifically detect anti-PRRSV-1 antibodies in the clinical pig ***,combining two nanobody-based cELISAs can differentially detect antibodies against PRRSV-1 and-2.

关键词： PRRSV Competitive ELISA Nanobody Antigen epitope

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分析师关注提高了企业的ESG表现吗

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现代经济探讨 2024年第2期 51-66页

作者：王雪刘清源对外经济贸易大学金融学院北京100029

以2011-2021年中国A股非金融类上市公司数据,实证考察了分析师关注对企业ESG表现的提升效应及其影响机制,并且这一促进作用在融资约束程度高、信息透明度低和盈余管理程度高的企业中更为明显。机制分析表明,分析师外部监督作用发挥的直... 详细信息

以2011-2021年中国A股非金融类上市公司数据,实证考察了分析师关注对企业ESG表现的提升效应及其影响机制,并且这一促进作用在融资约束程度高、信息透明度低和盈余管理程度高的企业中更为明显。机制分析表明,分析师外部监督作用发挥的直接效应和其信息中介作用吸引的特定投资者参与公司治理的间接效应协同发力,共同促进了企业ESG表现的提升。进一步分析发现,分析师关注在提高企业环境和治理分项得分方面的表现突出。此外,分析师调研以及调研过程中的绿色关注都对企业ESG表现有着明显的促进作用。进一步证实了证券分析师在中国资本市场上的重要作用,也为政策制定者构建企业ESG相关制度提供了决策依据。

关键词：分析师关注企业ESG表现信息中介外部监督

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中国未破裂颅内动脉瘤临床管理指南(2024版)解读

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中华医学杂志 2024年第21期104卷 1903-1906页

作者：赵兵刘清源王硕上海交通大学医学院附属仁济医院神经外科上海首都医科大学附属北京天坛医院神经外科北京

随着脑血管影像技术的普及, 未破裂颅内动脉瘤(UIA)临床检出率越来越高。UIA破裂风险低, 但是一旦破裂, 严重影响人类健康。UIA治疗存在较大争议, 引起临床广泛关注。中华医学会神经外科学分会、中国卒中学会脑血管外科分会、国家神经... 详细信息

随着脑血管影像技术的普及, 未破裂颅内动脉瘤(UIA)临床检出率越来越高。UIA破裂风险低, 但是一旦破裂, 严重影响人类健康。UIA治疗存在较大争议, 引起临床广泛关注。中华医学会神经外科学分会、中国卒中学会脑血管外科分会、国家神经系统疾病医学中心与国家神经系统疾病临床研究中心联合制订《中国未破裂颅内动脉瘤临床管理指南(2024版)》, 该指南采用模块化形式, 突出推荐意见, 并指出当前研究不足及未来研究方向, 从UIA流行病学、人群筛查、临床影像诊断、破裂风险评估、治疗决策及选择, 术后随访及长期管理等方面提供了全面的临床管理意见。将证据来源分为中国人群和其他人群, 有助于指导国内临床实践。

关键词：颅内动脉瘤指南破裂风险治疗临床管理

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基于人工智能的未破裂颅内动脉瘤形态学指标三维测量方法

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中国卒中杂志 2024年第1期19卷 112-119页

作者：杨溢刘清源刘伟奇王硕首都医科大学附属北京天坛医院神经外科北京100070 同心智医科技(北京)股份有限公司

未破裂颅内动脉瘤形态学分析被广泛用于动脉瘤破裂的风险评估和术前规划。三维视角较既往的二维视角更能精确体现动脉瘤的形态变化,但人工测量在三维视角下准确度较低且可重复性差,而人工智能在三维视角下的测量更有优势。但是,目前尚... 详细信息

未破裂颅内动脉瘤形态学分析被广泛用于动脉瘤破裂的风险评估和术前规划。三维视角较既往的二维视角更能精确体现动脉瘤的形态变化,但人工测量在三维视角下准确度较低且可重复性差,而人工智能在三维视角下的测量更有优势。但是,目前尚缺乏人工智能在三维视角下的动脉瘤形态测量方法。考虑到三维视角下的规范化测量对形态学参数的临床和科研运用具有重要意义,本文提出了一套三维视角下基于人工智能的未破裂颅内动脉瘤形态学指标测量方法,以期推动动脉瘤研究的同质化和临床诊疗的规范化发展。

关键词：颅内动脉瘤形态学指标人工智能三维

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“两个结合”形成的内在机理与价值意蕴

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兰州工业学院学报 2024年第4期31卷 109-113页

作者：刘青远宫留记河南大学马克思主义学院河南开封475001

“两个结合”是中国共产党人在百年奋斗中总结出来的科学理论,是习近平新时代中国特色社会主义思想的重要内容。“两个结合”重要论断将马克思主义基本原理与中国具体实际、中华优秀传统文化深度融合,是对马克思主义理论的守正创新。在... 详细信息

“两个结合”是中国共产党人在百年奋斗中总结出来的科学理论,是习近平新时代中国特色社会主义思想的重要内容。“两个结合”重要论断将马克思主义基本原理与中国具体实际、中华优秀传统文化深度融合,是对马克思主义理论的守正创新。在两个一百年奋斗目标的交汇期,“两个结合”的提出契合了时代发展的主题,为实现中华民族的伟大复兴提供了行动指南。深入分析“两个结合”的内在机理、价值意蕴,有利于加深对中国共产党创新理论的理解,在把握其旨归的基础上探索实践路径,推动社会主义现代化建设。

关键词：两个结合马克思主义中国化守正创新生成逻辑

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铋剂四联疗法联合针刺督脉背段压痛穴位治疗脾胃虚寒型Hp感染性胃溃疡疗效分析

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中国烧伤创疡杂志 2024年第2期36卷 152-156页

作者：王小沙刘青塬河南科技大学第一附属医院康复医学科河南洛阳471000

目的分析铋剂四联疗法联合针刺督脉背段压痛穴位治疗脾胃虚寒型幽门螺杆菌(Hp)感染性胃溃疡的临床疗效。方法选取2020年1月至2021年12月河南科技大学第一附属医院收治的80例脾胃虚寒型Hp感染性胃溃疡患者作为研究对象,按照不同治疗方法... 详细信息

目的分析铋剂四联疗法联合针刺督脉背段压痛穴位治疗脾胃虚寒型幽门螺杆菌(Hp)感染性胃溃疡的临床疗效。方法选取2020年1月至2021年12月河南科技大学第一附属医院收治的80例脾胃虚寒型Hp感染性胃溃疡患者作为研究对象,按照不同治疗方法将其分为联合组(40例)与对照组(40例),联合组患者采用铋剂四联疗法联合针刺督脉背段压痛穴位治疗,对照组患者单纯采用铋剂四联疗法治疗,对比观察两组患者中医症候评分、溃疡长径、血清胃泌素(GAS)水平、Hp转阴率及临床疗效。结果治疗14 d后,联合组患者胃脘疼痛、食后腹胀、嗳气泛酸、恶心反胃评分以及血清GAS水平均明显低于对照组(t=6.910、6.318、7.140、9.345、8.819,P均<0.001),溃疡长径明显短于对照组(t=18.860,P<0.001);联合组患者Hp转阴率为87.5%,明显高于对照组患者的Hp转阴率67.5%(χ^(2)=4.588,P=0.032);联合组患者中显效26例、有效11例、无效3例,明显优于对照组患者的显效19例、有效10例、无效11例(Z=-1.997,P=0.046)。结论铋剂四联疗法联合针刺督脉背段压痛穴位治疗脾胃虚寒型Hp感染性胃溃疡,可明显降低GAS水平,提高Hp转阴率,改善患者脾胃虚寒症状,促进溃疡愈合。

关键词：针刺督脉背段压痛穴位铋剂四联疗法 Hp感染胃溃疡脾胃虚寒

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High Order Finite Difference WENO Methods for Shallow Water Equations on Curvilinear Meshes

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Communications on Applied Mathematics and Computation 2023年第1期5卷 485-528页

作者： Zepeng liu Yan Jiang Mengping Zhang qingyuan liu School of Mathematical Sciences University of Science and Technology of ChinaHefei 230026AnhuiChina School of Mathematics and Physics Anhui Jianzhu UniversityHefei 230026AnhuiChina

A high order finite difference numerical scheme is developed for the shallow water equations on curvilinear meshes based on an alternative flux formulation of the weighted essentially non-oscillatory(WENO)*** exact C-property is investigated,and comparison with the standard finite difference WENO scheme is *** derivation and numerical results show that the proposed finite difference WENO scheme can maintain the exact C-property on both stationarily and dynamically generalized coordinate *** Harten-Lax-van Leer type flux is developed on general curvilinear meshes in two dimensions and verified on a number of benchmark problems,indicating smaller errors compared with the Lax-Friedrichs *** addition,we propose a positivity-preserving limiter on stationary meshes such that the scheme can preserve the non-negativity of the water height without loss of mass conservation.

关键词： Shallow water equation Well-balanced High order accuracy WENO scheme Curvilinear meshes Positivity-preserving limiter

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Azimuthal Variations of the Convective-scale Structure in a Simulated Tropical Cyclone Principal Rainband

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Advances in Atmospheric Sciences 2020年第11期37卷 1239-1255页

作者： Yue JIANG Liguang WU Haikun ZHAO Xingyang ZHOU qingyuan liu Key Laboratory of Meteorological Disaster of Ministry of Education Nanjing University of Information Science and TechnologyNanjing 210044China State Key Laboratory of Severe Weather Chinese Academy of Meteorological SciencesBeijing 100081China Department of Atmospheric and Oceanic Sciences and Institute of Atmospheric Sciences Fudan UniversityShanghai 200433China Nanjing Joint Institute for Atmospheric Sciences Nanjing 210009China

Previous numerical simulations have focused mainly on the mesoscale structure of the principal rainband in tropical cyclones with a relatively coarse model *** this study,the principal rainband was simulated in a semi-idealized experiment at a horizontal grid spacing of 1/9 km and its convective-scale structure was examined by comparing the convective elements of the simulated principal rainband with previous observational *** is found that the convective scale structure of the simulated principal rainband is well comparable to the *** azimuthal variations of the convective scale structure were examined by dividing the simulated principal rainband into the upwind,middle and downwind *** new features are found in the simulated principal ***,the overturning updraft contains small-scale rolls aligned along the inward side of the outward-leaning reflectivity tower in the middle ***,the inner-edge downdraft is combined with a branch of inflow from the upper levels in middle and downwind portions,carrying upper-level dry air to the region between the overturning updrafts and eyewall,and the intrusion of the upper-level dry air further limits the altitude of the overturning updrafts in the middle and downwind portions of the principal ***,from the middle to downwind portions,the strength of the secondary horizontal wind maximum is gradually replaced by a low-level maximum of the tangential wind collocated with the low-level downdraft.

关键词： azimuthal variations principal rainband tropical cyclone WRF-LES simulation

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