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Genetic characterizations of Toll-like receptors in the brown rat and their associations with pathogen infections

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Integrative Zoology 2022年第5期17卷 879-889页

作者： Qianqian SU Yi CHEN Bo WANG qingxun zhang Hongxuan HE National Research Center for Wildlife-Borne Diseases Chinese Academy of SciencesInstitute of ZoologyBeijingChina University of Chinese Academy of Sciences BeijingChina State Key Laboratory of Integrated Management of Pest Insects and Rodents Chinese Academy of SciencesInstitute of ZoologyBeijingChina

Toll-like receptors(TLRs)are important initiators of innate immune responses that target host-pathogen ***,further research into the molecular characteristics of TLRs in wild populations is required,as well as how TLRs genetically influenced pathogen infections in the brown rat(Rattus norvegicus).Here,we explored the genetic characterization and evolution of 2 sensing nucleic acid TLRs(TLR7 and TLR8)and 2 sensing nonnucleic acid TLRs(TLR2 and TLR4)in the wild brown rat,and assessed their associations with 2 RNA viruses(Seoul hantavirus and rat hepatitis E virus(HEV))and 2 bacteria(Leptospira and Bartonella).In these 4 TLRs,we discovered a total of 16 ***,TLR8 had high genetic diversity among 7 variants,while TLR2 had low genetic diversity with only 1 *** to selective pressure analyses,TLR4,TLR7,and TLR8 genes evolved under purifying ***,significant associations were found between 3 TLR8 variants and HEV infection,as well as 1 TLR2 variant and Bartonella ***,our findings provided a glimpse into the genetic characterization of TLRs in the brown rat,and further demonstrated that TLR2 and TLR8 genetic variations were related to Bartonella and HEV infection,***,TLR8 may be a good candidate immune gene for future research on molecular ecology and functional adaptation in wild populations.

关键词： Bartonella brown rats hepatitis E virus polymorphism susceptibility Toll-like receptors

Surface pseudocapacitance of mesoporous Mo_(3)N_(2) nanowire anode toward reversible high-rate sodium-ion storage

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Journal of Energy Chemistry 2021年第4期30卷 295-303页

作者： Yalong Jiang Jun Dong Shuangshuang Tan Qiulong Wei Fangyu Xiong Wei Yang Yuanhao Shen qingxun zhang Zi'ang Liu Qinyou An Liqiang Mai State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070HubeiChina Department of Materials Science and Engineering Fujian Key Laboratory of Materials GenomeCollege of MaterialsXiamen UniversityXiamen 361005FujianChina Foshan Xianhu Laboratory of the Advanced Energy Science and Technology Guangdong Laboratory Xianhu Hydrogen ValleyFoshan 528200GuangdongChina

Sodium-ion storage devices are highly desirable for large-scale energy storage applications owing to the wide availability of sodium resources and low *** metal nitrides(TMNs)are promising anode materials for sodium-ion storage,while their detailed reaction mechanism remains ***,we synthesize the mesoporous Mo3N2 nanowires(Meso-Mo_(3)N_(2)-NWs).The sodium-ion storage mechanism of Mo3N2 is systematically investigated through in-situ XRD,ex-situ experimental characterizations and detailed kinetics ***,the Mo_(3)N_(2) undergoes a surface pseudocapacitive redox charge storage *** from the rapid surface redox reaction,the Meso-Mo_(3)N_(2)-NWs anode delivers high specific capacity(282 m Ah g^(-1) at 0.1 A g^(-1)),excellent rate capability(87 m Ah g^(-1) at 16 A g^(-1))and long cycling stability(a capacity retention of 78.6%after 800 cycles at 1 A g^(-1)).The present work highlights that the surface pseudocapacitive sodium-ion storage mechanism enables to overcome the sluggish sodium-ion diffusion process,which opens a new direction to design and synthesize high-rate sodiumion storage materials.

关键词： Surface pseudocapacitance Sodium-ion storage Nitrogen vacancy Molybdenum nitride High-rate

Identification and genetic characterization of Contracaecum sp.(Nematoda:Anisakidae)from China

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Integrative Zoology 2021年第6期16卷 929-938页

作者： qingxun zhang Meng MENG Chengmei HUANG Shengyong FENG Jie LIU Yan TANG Yu FAN Guohui YUAN Shuyi HAN Jing LUO Baohua ZHAO Hongxuan HE National Research Center for Wildlife-Borne Diseases Institute of ZoologyChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina College of Wildlife Resources Northeast Forestry UniversityHaerbinHeilongjiangChina China Wildlife Conservation Association BeijingChina Hebei Normal University ShijiazhuangHebeiChina

Contracaecum species are economically important fish-borne larval nematodes with zoonotic *** June 2019,more than 100 piscivorous birds died in their habitats close to the Wild Duck Lake,located in Yanqing,northwest of Beijing,***-mortem examination of Black Night Herons(Nycticorax)revealed the presence of numerous anisakid nematodes in the *** nematodes were identified as Contracaecum *** on morphological *** analysis of the mitochondrial(mt)genome and the ITS gene showed that sequences of Contracaecum *** isolates were grouped into a new individual ***,the parasite was successfully isolated from fresh dead birds,feces of piscivorous birds,and fish and prevalence ranged from 8.0%to 81.8%.Consequently,our study demonstrated Contracaecum *** in different sources from China,which might constitute a threat to wildlife,aquaculture,and public health.

关键词： Anisakid epidemiology morphology phylogenetic analysis

聚合羟基铝改性膨润土催化合成尼龙酸二异丁酯的研究

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精细石油化工 2005年第6期22卷 6-8页

作者：金振兴孙曙光张庆勋渤海大学应用化学辽宁省重点实验室辽宁锦州121013

以聚合羟基铝与膨润土进行交联反应制备聚合羟基铝改性膨润土酯化催化剂,并用于催化合成尼龙酸二异丁酯。通过正交实验确定的最佳反应条件为:酸醇摩尔比为1∶2.5,催化剂为反应物总量的2.3%,反应回流3.0h,在此条件下酯化率可达96.5%。

关键词：聚合羟基铝膨润土尼龙酸二异丁酯

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井筒信息一体化地层建模及地质导向技术

测井技术 2021年第3期45卷 227-232页

作者：邵才瑞翟星雨原野张福明张庆勋中国石油大学(华东)地球科学与技术学院山东青岛266580 青岛海洋科学与技术国家实验室-海洋矿产资源评价与探测技术功能实验室山东青岛266580 中石油测井重点实验室中国石油大学(华东)研究室山东青岛266580 深层油气重点实验室山东青岛266580

利用随钻测井资料进行地质导向是定向钻井的前沿技术,由于目前随钻测井资料基本以无方位自然伽马、电阻率资料为主,因此,存在地层建模和地质导向的不确定性。鉴于录井资料可提供钻遇地层的原始资料,钻时和钻压资料可间接反映岩性和缝洞... 详细信息

利用随钻测井资料进行地质导向是定向钻井的前沿技术,由于目前随钻测井资料基本以无方位自然伽马、电阻率资料为主,因此,存在地层建模和地质导向的不确定性。鉴于录井资料可提供钻遇地层的原始资料,钻时和钻压资料可间接反映岩性和缝洞异常等,综合利用随钻测井、录井和钻井资料对钻遇地层进行评价和建模可以弥补单一随钻测井资料的不足。介绍了井筒信息和地层模型一体可视化技术,以及综合利用测井、录井、钻井资料进行储层评价、地层模型精细刻画和地质导向的方法与流程。应用实例表明,综合利用测井、录井、钻井资料进行地层精细刻画建模及地质导向是在缺少方位随钻测井资料条件下减少地层模型不确定性的有效方法。

关键词：井筒信息综合可视化地层评价地质导向

麋鹿角柄发育规律与相关内分泌及信号通路检测

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野生动物学报 2023年第3期44卷 565-571页

作者：夏志强单云芳李俊芳张庆勋贺永惠崔艳红钟震宇柏超张成林白加德孟庆辉北京市科学技术研究院北京麋鹿生态实验中心北京100076 河南科技学院动物科技学院新乡453003 圈养野生动物技术北京市重点实验室北京动物园管理处北京100044

鹿类角柄终生有2次发育,第1次在胚胎期,第2次在青春期,出生后角柄的激活和首次发育对于鹿角再生极为重要,角柄的青春期发育对于鹿类个性及等级序位形成、鹿角大小、争夺配偶、种间竞争和应对天敌等有重要意义。利用红外测距仪,开展麋鹿(... 详细信息

鹿类角柄终生有2次发育,第1次在胚胎期,第2次在青春期,出生后角柄的激活和首次发育对于鹿角再生极为重要,角柄的青春期发育对于鹿类个性及等级序位形成、鹿角大小、争夺配偶、种间竞争和应对天敌等有重要意义。利用红外测距仪,开展麋鹿(Elaphurus davidianus)角柄生长发育的宏观测量,结合青春期骨膜组织取样、血液激素水平和信号通路表达情况检测,对麋鹿角柄发育情况进行研究。结果表明:与成年不同,幼龄雄性麋鹿在第1年秋季开始出生后的首次角柄发育,由毛旋处长出骨质突起,翌年2—3月角柄长至2.5~3.0 cm,逐渐形成初角基,5—6月首次生茸,冬至脱落,此时形成完整的角柄;随青春期发育和年龄增长,角柄逐渐变粗变短;角柄围长由2岁时的(17.17±1.00)cm增加到3岁时的(35.43±0.83)cm,差异极显著(F_(1,9)=4.1281,n=9,p=0.0053);角柄直径由2岁时的(3.66±0.59)cm增加到3岁时的(5.91±0.72)cm,差异极显著(F_(1,9)=3.1740,n=9,p=0.0246)。血液激素检测表明睾酮分泌水平与角柄发育密切相关,睾酮分泌水平由1岁时的(564.27±41.16)pg/m L增加到3岁时的(737.96±66.57)pg/m L,差异极显著(F_(1,9)=4.3030,n=9,p=0.0022);分子检测结果也显示TGF-β/Smads信号通路参与了角柄发育过程。研究揭示了麋鹿角柄发育规律,探讨与之相关的内分泌因素及分子表达情况,为今后持续开展麋鹿角柄发育及茸再生机制多样化奠定了基础。

关键词：角柄发育麋鹿内分泌信号通路 TGF-β Smads

DYNAMIC CHARACTERISTICS OF AXIALLY SYMMETRIC SUPERSONIC FLOW UNDER MICROWAVE CONDITIONS

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DYNAMIC CHARACTERISTICS OF AXIALLY SYMMETRIC SUPERSONIC FLOW...

基于CRISPR-Cas系统的病原体检测研究进展

2002 3rd International Conference on Microwave and Millimeter Wave Technology

作者： Zhao qingxun Ding Wenge zhang Kaixi College of Physics Science and Technology Hebei University

ISBN: (纸本)078037486X

＜正＞Using supersonic dynamic and Onsager potential transfer differential equation of irreversible process thermodynamic,dynamic characteristics of axially symmetric supersonic flow radiated by microwave beam has been studied *** results corresponding to this research are relatively satisfied in comparison with experimental *** is useful to study parameters of the plasma by ultrasonic method for the plasma diagnostics.

关键词： irreversible thermodynamics supersonic flow axial symmetry plasma diagnostics plasma interactions plasma radiofrequency heating microwave beam radiated axially symmetric supersonic flows supersonic dynamics Onsager potential transfer differential equation irreversible process thermodynamics supersonic flow dynamic characteristics ultrasonic plasma parameter study method plasma diagnostics plasma interactions

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中国畜牧兽医 2022年第8期49卷 3190-3199页

作者：张庆勋钟震宇郭青云何宏轩白加德北京麋鹿生态实验中心北京100076 北京生物多样性保护研究中心北京100076 中国科学院动物研究所北京100101

开发快速、灵敏、特异的检测方法对于新发突发人兽共患病、动物疫病的监测及防控至关重要。规律成簇的间隔短回文重复序列及相关蛋白(CRISPR-Cas)系统以高敏感性、特异性及可扩展性的DNA和RNA识别为特点,已成为新一代的分子诊断工具,在... 详细信息

开发快速、灵敏、特异的检测方法对于新发突发人兽共患病、动物疫病的监测及防控至关重要。规律成簇的间隔短回文重复序列及相关蛋白(CRISPR-Cas)系统以高敏感性、特异性及可扩展性的DNA和RNA识别为特点,已成为新一代的分子诊断工具,在病原体快速检测技术研发中得到广泛应用。在新型冠状病毒肺炎疫情中,基于CRISPR-Cas系统开发的新型冠状病毒(SARS-CoV-2)检测试剂盒(SHERLOCK和DETECTR)在美国相继获批,中国研发的基于CRISPR-Cas12系统的SARS-CoV-2检测试剂盒也已获批上市,这表明基于CRISPR-Cas系统的病原体检测方法在未来的应用前景非常可观。文章首先简述了CRISPR-Cas系统类型,尤其是常用于病原体检测的Cas9、Cas12和Cas13;介绍了CRISPR-Cas系统的作用机理以及广泛应用。同时分析了CRISPR-Cas检测平台研发的关键技术问题,包括靶标基因的序列保守性、靶标基因的富集与扩增、终信号不同的读取方式等,并对CRISPR-Cas系统的未来发展及应用前景进行了展望。

关键词： CRISPR-Cas系统病原体检测诊断应用

水貂源铜绿假单胞菌的分离鉴定、耐药性及毒力基因检测

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中国畜牧兽医 2021年第6期48卷 2230-2237页

作者：张庆勋景胜凡韩姝伊王若琪冯胜勇何宏轩中国科学院动物研究所北京100101 河北农业大学保定071001 沈阳工学院抚顺113122 中国科学院大学北京100049

为查明河北某水貂养殖场水貂死亡原因,本研究进行了病理组织学检查、细菌分离培养、细菌形态学观察、生化鉴定、16S rDNA鉴定、药敏试验、耐药基因和毒力基因检测、动物回归试验等。结果显示,共分离到3株铜绿假单胞菌,16S rDNA序列与Gen... 详细信息

为查明河北某水貂养殖场水貂死亡原因,本研究进行了病理组织学检查、细菌分离培养、细菌形态学观察、生化鉴定、16S rDNA鉴定、药敏试验、耐药基因和毒力基因检测、动物回归试验等。结果显示,共分离到3株铜绿假单胞菌,16S rDNA序列与GenBank中铜绿假单胞菌相应序列相似性达到100%,且3株分离菌均能使大鼠死亡。药敏结果表明,分离菌对左氧氟沙星、庆大霉素、阿米卡星、多黏菌素B敏感,对β-内酰胺类、氨基糖苷类、大环内酯类、喹诺酮类、四环素类等抗生素耐药。分离菌携带3种超广谱β-内酰胺酶基因(bla_(CTX-M1)、bla_(OXA-2)、bla_(OXA-10))、3种碳青霉烯酶基因(bla_(VIM-1)、bla_(SPM-1)、bla_(KPC-1))及吸附相关基因、T1SS-T3SS分泌系统、氧化应激相关基因、群体行为调控基因、磷脂酶相关的17种毒力基因。本研究结果为水貂绿脓杆菌病的临床诊断和治疗提供了参考依据。

关键词：水貂绿脓杆菌分离鉴定耐药性