检索结果-南通市图书馆

Animal Nutrition 2024年第1期16卷 251-266页

作者： Pengju Wang Lv-hui Sun Xu Wang qinghua wu Aimei Liu Hubei Key Laboratory of Diabetes and Angiopathy Medicine Research Institute Xianning Medical College Hubei University of Science and Technology Hubei Hongshan Laboratory College of Animal Science and Technology Huazhong Agricultural University National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues Huazhong Agricultural University College of Life Science Yangtze University

T-2 toxin is one of the most widespread and toxic fungal toxins in food and feed. It can cause gastrointestinal toxicity, hepatotoxicity, immunotoxicity, reproductive toxicity, neurotoxicity, and nephrotoxicity in humans and animals. T-2 toxin is physicochemically stable and does not readily degrade during food and feed processing. Therefore, suppressing T-2 toxin-induced organ toxicity through antidotes is an urgent issue. Protective agents against the organ toxicity of T-2 toxin have been recorded widely in the literature, but these protective agents and their molecular mechanisms of detoxification have not been comprehensively summarized. In this review, we provide an overview of the various protective agents to T-2 toxin and the molecular mechanisms underlying the detoxification effects. Targeting appropriate targets to antagonize T-2 toxin toxicity is also an important option. This review will provide essential guidance and strategies for the better application and development of T-2 toxin antidotes specific for organ toxicity in the future.

关键词： T-2 toxin Organ toxicity Protective agent Detoxification mechanism

Foodborne toxin Aflatoxin B_(1)induced glomerular podocyte inflammation through proteolysis of RelA,downregulation of miR-9 and CXCR4/TXNIP/NLRP3 pathway

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年第4期13卷 2289-2309页

作者： Jie Zhang Shuang Yang Baocai Xu Zihui Qin Xinyi Guo Ben Wei qinghua wu Kamil Kuca Tushuai Li Wenda wu School of Biology and Food Engineering Changshu Institute of TechnologySuzhou 215500China Center for Clinical Mass Spectrometry School of Pharmaceutical SciencesSoochow UniversitySuzhou 215123China School of Food and Biological Engineering Hefei University of TechnologyHefei 230009China MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095China Department of Chemistry Faculty of ScienceUniversity of Hradec KraloveHradec Kralove 50003Czech Republic College of Life Science Yangtze UniversityJingzhou 434025China wuxi Medical College Jiangnan UniversityWuxi 214122China wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute wuxi Branch Wuxi 214013China

Aflatoxin B_(1)(AFB_(1))is a naturally-occurring mycotoxin and recognized as the most toxic foodborne toxin,particularly causing damages to kidney.Glomerular podocytes are terminally differentiated epithelial cells.AFB_(1)induces podocyte inflammation,proteinuria and renal dysfunction.Studying the mechanism of AFB_(1)-induced podocyte inflammation and murine kidney dysfunction,we detected that AFB_(1)increased ubiquitindependent degradation of the transcription factor RelA through enhanced interaction of RelA with E3 ubiquitin ligase tripartite motif containing 7(TRIM7)in mouse podocyte clone-5(MPC-5)and mouse glomeruli.Reduction of RelA resulted in decreasing microRNA-9(miR-9)and activating the chemokine receptor 4(CXCR4),thioredoxin interacting protein(TXNIP),and NOD-like receptor pyrin domain-containing 3(NLRP3)signaling axis(CXCR4/TXNIP/NLRP3 pathway),leading to podocyte inflammation.We also determined that downregulation of miR-9 led to CXCR4 expression and the downstream TXNIP/NLRP3 pathway activation.Overexpression of miR-9 or deletion of CXCR4 suppressed AFB_(1)-induced CXCR4/TXNIP/NLRP3 pathway,resulting in alleviating podocyte inflammation and kidney dysfunction.Our findings indicated that ubiquitin-dependent proteolysis of RelA,downregulation of miR-9,and activation of CXCR4/TXNIP/NLRP3 pathway played an essential role in AFB_(1)-induced glomerular podocyte inflammation.Our study revealed a novel mechanism,via RelA,for the control of AFB_(1)’s nephrotoxicity,leading to an effective protection of food safety and public health.

关键词： Aflatoxin B_(1) Podocyte inflammation miRNA-9 Chemokine(C-X-C motif)receptor 4 RelA ubiquitin-dependent degradation

Cytochrome P450 enzymes mediated by DNA methylation is involved in deoxynivalenol-induced hepatoxicity in piglets

在线全文

学校读者我要写书评

暂无评论

Animal Nutrition 2022年第2期9卷 269-279页

作者： Aimei Liu Yaqin Yang Jingchao Guo Yan Gao qinghua wu Ling Zhao Lv-hui Sun Xu Wang National Reference Laboratory of Veterinary Drug Residues and Key Laboratory for Detection of Veterinary Drug Residues Huazhong Agricultural UniversityMinistry of Agriculture(MOA)Wuhan430070China Hubei Hongshan Laboratory College of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan430070China MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Wuhan430070China College of Life Science Yangtze UniversityJingzhou434025China

Deoxynivalenol(DON)is an inevitable contaminant in animal feed and can lead to liver damage,then decreasing appetite and causing growth retardation in piglets.Although many molecular mechanisms are related to hepatoxicity caused by DON,few studies have been done on cytochrome P450(CYP450)enzymes and DNA methylation.To explore the role of CYP450 enzymes and DNA methylation in DONinduced liver injury,male piglets were fed a control diet,or diet containing 1.0 or 3.0 mg/kg DON for 4 weeks.DON significantly raised the activity of aspartate aminotransferase(AST),alanine aminotransferase(ALT),and glutamyl transpeptidase(GGT)(P<0.01),leading to liver injury.In vivo study found that DON exposure increased the expression of CYP450 enzymes(such as CYP1A1,CYP2E1,CYP3A29)(P<0.05),and disturbed the expression of nicotinamide N-methyltransferase(NNMT),galanin-like peptide(GALP)and insulin-like growth factor 1(IGF-1)(P<0.05),in which DNA methylation affected the expression of these genes.In vitro study(human normal hepatocytes L02)further proved that DON elevated the expression of CYP1A1,CYP2E1 and CYP3A4(P<0.05),and inhibited cell growth in a dose-dependent manner,resulting in cell necrosis.More importantly,knockdown of CYP1A1 or CYP2E1 could alleviate DON-induced growth inhibition by promoting IGF-1 expression.Taken together,increased CYP450 enzymes expression was one of the mechanisms of hepatoxicity and growth inhibition induced by DON,suggesting that the decrease of CYP450 enzymes can antagonize the hepatoxicity in animals,which provides some value for animal feed safety.

关键词： Deoxynivalenol Hepatoxicity Growth inhibition CYP450 enzyme DNA methylation Piglet

Metabolomic and proteomic responses of Phaeodactylum tricornutum to hypoxia

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2022年第5期40卷 1963-1973页

作者： Peipei ZHAO qinghua wu Xuekui XIA Shiyi GUO Sizhong SHEN Yujue WANG Aiyou HUANG Key Biosensor Laboratory of Shandong Province Biology InstituteQilu University of Technology(Shandong Academy of Sciences)Jinan 250103China State Key Laboratory of Marine Resource Utilization in the South China Sea Hainan UniversityHaikou 570228China Laboratory of Development and Utilization of Marine Microbial Resource Hainan UniversityHaikou 570228China Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province Haikou 570228China College of Marine Sciences Hainan UniversityHaikou 570228China Department of Chemistry University of WashingtonSeattle WA 98195USA

Diatoms are important contributors to global net primary productivity,and play a crucial role in the biogeochemical cycles of carbon,phosphorus,nitrogen,iron,and silicon.Currently in some regions in the ocean,there’s a trend that carbon content is high while oxygen concentration is low,and the underlying mechanisms of diatoms’response to low oxygen environments are worth investigating.Phaeodactylum tricornutum is a model diatom whose genome has been sequenced;it provides a universal molecular toolbox and a stable transgenic expression system.Therefore,the study of the responses of P.tricornutum to low oxygen has not only fundamental research significance but also important ecologic al significance.In this study,growth rates were determined and proteomic analysis and metabolomic analysis were performed to examine P.tricornutum responses under different oxygen concentrations(2%oxygen concentration for hypoxic condition and 21%oxygen concentration for the normal condition(sterilized air)).Results show that the hypoxic environment inhibited the growth of P.tricornutum.In the hypoxic conditions,P.tricornutum could reset its metabolism pathways,including enhancement in lipid utilization,replenishment of tricarboxylic acid(TCA)cycle through the glyoxylic acid cycle,and down-regulation of photorespiration to reduce energy waste.Additionally,the stress resistance mechanism was activated to facilitate the adaptation to low oxygen conditions.This study helps to reveal the different metabolic changes to hypoxia of diatom from that of higher plants,which might be ascribed to their different habitats and needs further exploration in the future.

关键词： Phaeodactylum tricornutum hypoxia metabolomics proteomics

Health： a dream from reality to the future

在线全文

学校读者我要写书评

暂无评论

Frontiers of Medicine 2016年第2期10卷 233-235页

作者： Chunsong Hu qinghua wu Department of Cardiovascular Medicine Nanchang University Hospital Nanchang University Nanchang 330006 China

这份报纸主张健康是可以提高幸福的索引的现实主义的生产力量。作为所有开发的基础和来源一人和其家庭幸福，健康要求不仅主要、第二等的预防，而且政策预防，那是说，等级 -- 零预防。因此，人们应该在所有政策对健康给予更多的注意。... 详细信息

这份报纸主张健康是可以提高幸福的索引的现实主义的生产力量。作为所有开发的基础和来源一人和其家庭幸福，健康要求不仅主要、第二等的预防，而且政策预防，那是说，等级 -- 零预防。因此，人们应该在所有政策对健康给予更多的注意。作为新预防策略，政策预防将帮助显著地改进民族健康并且支持健康中国和中国梦或世界梦的概念从现实实现一个梦到未来。

关键词：健康幸福指数预防生产力中国

Research progresses and prospects of medicated oil dual-purpose crop safflower based on patent mining

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oil Crop Science 2022年第4期7卷 209-218页

作者： Lanyu Zhou Yuhang Yan Yue Wang qinghua wu Jie Yan Jin Pei State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China Chengdu611137China School of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu611137China

Safflower is widely cultivated and used as a dual-purpose medicinal oil worldwide.This research used the incoPat patent database to search the global safflower patents for nearly 20 years,aiming to reveal the development process and current situation of the safflower industry in China and around the world by analyzing patent application trends,patent technology characteristics,advantageous industries and regions,patent quality,etc.The analysis shows that safflower patent applications are generally increasing,and the period 2010-2016 is characterized by the rapid development of patent applications.The technical features of global patents are mainly concentrated in pharmaceutical preparations,cosmetics,food and health care,various beverages,animal feed,safflower cultivation and harvesting equipment,and safflower seed oil.During the rapid development of patent applications,safflower was further developed in cosmetics,edible oil,and harvesting equipment.In addition,patent applications in rheumatism and bone diseases have increased,with the potential for development.China is the leading region for researching and developing medicinal safflower patents,especially Shandong and Henan provinces.Foreign patents are more focused on the value development of safflower seed oil in food and chemicals.Compared to the major countries and organizations that apply for safflower patents on a global scale,the quality of Chinese safflower patents is medium,and more awareness of patent protection is required.This paper provides suggestions for the subsequent development of the safflower industry,summarizes and analyzes the development trend of safflower patents,and provides new ideas to obtain innovative patent results.

关键词： Safflower Patent Medicated oil dual-purpose crop Industry incoPat

Cloning,expression and activity analysises of chalcone synthase genes in Carthamus tinctorius

在线全文

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2023年第2期15卷 291-297页

作者： Xiaohui Tang Chaoxiang Ren Jing Hu Jiang Chen Jie Wang Rui Wang qinghua wu Wan Liao Jin Pei State Key Laboratory of Southwestern Chinese Medicine Resources Chengdu University of Traditional Chinese MedicineChengdu 611137China College of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu 611137China Sichuan Academy of Grassland Sciences Chengdu 611731China

Objective:Flavonoids are the bioactive compounds in safflower(Carthamus tinctorius),in which chalcone synthase(CHS)is the first limiting enzyme.However,it is unclear that which chalcone synthase genes(CHSs)are participated in flavonoids biosynthesis in C.tinctorius.In this study,the CHSs in the molecular characterization and enzyme activities were investigated.Methods:Putative chalcone biosynthase genes were screened by the full-length transcriptome sequences data in C.tinctorius.Chalcone biosynthase genes in C.tinctorius(CtCHSs)were cloned from cDNA of flowers of C.tinctorius.The cloned gene sequences were analyzed by bioinformatics,and their expression patterns were analyzed by real-time PCR(RT-PCR).The protein of CtCHS in the development of flowers was detected by polyclonal antibody Western blot.A recombinant vector of CtCHS was constructed.The CtCHS recombinant protein was induced and purified to detect the enzyme reaction(catalyzing the reaction of p-coumaryl-CoA and malonyl-CoA to produce naringin chalcone).The reaction product was detected by HPLC and LC-MS.Results:Two full-length CtCHS genes were successfully cloned from the flowers of safflower(CtCHS1 and CtCHS3),with gene lengths of 1525 bp and 1358 bp,respectively.RT-PCR analysis showed that both genes were highly expressed in the flowers,but the expression of CtCHS1 was higher than that of CtCHS3 at each developmental stage of the flowers.WB analysis showed that only CtCHS1 protein could be detected at each developmental stage of the flowers.HPLC and LC-MS analyses showed that CtCHS1 could catalyze the conversion of p-coumaryl-CoA and malonyl-CoA substrates to naringin chalcone.Conclusion:CtCHS1 is involved in the biosynthesis of naringin chalcone in safflower.

关键词： Carthamus tinctorius L. chalcone synthase expression analysis flavonoids functional identification gene cloning safflower

Optimal reactive power dispatch with wind power integrated using group search optimizer with intraspecific competition and le´vy walk

在线全文

学校读者我要写书评

暂无评论

Journal of Modern Power Systems and Clean Energy 2014年第4期2卷 308-318页

作者： Yuanzheng LI Mengshi LI qinghua wu Department of Electric Power Engineering South China University of Technology(SCUT)Guangzhou 510641GuangdongChina Department of Electrical Engineering and Electronics University of LiverpoolLiverpool L693GJUK

This paper presents the mean–variance(MV)model to solve power system reactive power dispatch problems with wind power integrated.The MV model considers the profit and risk simultaneously under the uncertain wind power(speed)environment.To describe this uncertain environment,the Latin hypercube sampling with Cholesky decomposition simulation method is used to sample uncertain wind speeds.An improved optimization algorithm,group search optimizer with intraspecific competition and le´vy walk,is then used to optimize the MV model by introducing the risk tolerance parameter.The simulation is conducted based on the IEEE 30-bus power system,and the results demonstrate the effectiveness and validity of the proposed model and the optimization algorithm.

关键词： Mean-variance model Reactive power dispatch Wind power Group search optimizer with intraspecific competition and le´vy walk(GSOICLW)

Stochastic optimization of cost-risk for integrated energy system considering wind and solar power correlated

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Modern Power Systems and Clean Energy 2019年第6期7卷 1472-1483页

作者： Jiehui ZHENG Yanni KOU Mengshi LI qinghua wu School of Electric Power Engineering South China University of TechnologyGuangzhou 510640China

Due to the growing penetration of renewable energies(REs)in integrated energy system(IES),it is imperative to assess and reduce the negative impacts caused by the uncertain REs.In this paper,an unscented transformation-based mean-standard(UT-MS)deviation model is proposed for the stochastic optimization of cost-risk for IES operation considering wind and solar power correlated.The unscented transformation(UT)sampling method is adopted to characterize the uncertainties of wind and solar power considering the correlated relationship between them.Based on the UT,a mean-standard(MS)deviation model is formulated to depict the trade-off between the cost and risk of stochastic optimization for the IES optimal operation problem.Then the UT-MS model is tackled by a multi-objective group search optimizer with adaptive covariance and Levy flights embedded with a multiple constraints handling technique(MGSO-ACL-CHT)to ensure the feasibility of Peratooptimal solutions.Furthermore,a decision-making method,improved entropy weight(IEW),is developed to select a final operation point from the set of Perato-optimal solutions.In order to verify the feasibility and efficiency of the proposed UT-MS model in dealing with the uncertainties of correlative wind and solar power,simulation studies are conducted on a test IES.Simulation results show that the UT-MS model is capable of handling the uncertainties of correlative wind and solar power within much less samples and less computational burden.Moreover,the MGSOACL-CHT and IEW are also demonstrated to be effective in solving the multi-objective UT-MS model of the IES optimal operation problem.

关键词： Integrated energy system Renewable energies Unscented transformation Mean-standard deviation Multi-objective optimization Decision making

Nitrate Removal by a Permeable Reactive Barrier of Fe0:A Model-Based Evaluation

在线全文

学校读者我要写书评

暂无评论

Journal of Earth Science 2017年第3期28卷 447-456页

作者： qinghua wu Chunmiao Zheng Jiafa Zhang Fawang Zhang Changjiang River Scientific Research Institute Wuhan 430010China Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological SciencesShijiazhuang 050061China Department of Geological Sciences University of AlabamaTuscaloosaAlabama 35487USA Institute of Karst Geology Chinese Academy of Geological SciencesGuilin 541004China

Permeable reactive barrier（PRB） filled with zero valent iron（ZVI, Fe0） can be an effective option to remove nitrate from contaminated groundwater. The long-term performance of such PRBs, however, might be compromised by the problem of declining reactivity and permeability, which could cause a decrease in the nitrate removal efficiency. In this study we explored suitable model formulations that allow for a process-based quantification of the passivation effect on denitrification rates and tested the model for a 40 years long operation scenario. The conceptual model underlying our selected formulation assumes the declining reactivity of the ZVI material through the progressing passivation caused by the precipitation of secondary minerals and the successive depletion of the ZVI material. Two model scenarios, i.e., the base model scenario which neglects the explicit consideration of the passivation effect and one performed with the model in which the impact of the passivation effect on denitrification was considered, were compared. The modeling results illustrate that nitrate removal in the model of considered passivation started to be incomplete after 10 years, and the effluent nitrate concentration of PRB rose up to 86% of the injected water concentration after 40 years, in contrast to the base scenario, corresponding well with the field observations of successively declining nitrate removal efficiencies. The model results also showed that the porosity of the PRB increased in both models. In order to improve and recover the reactivity of ZVI, pyrite was added to the PRB, resulting in completely nitrate removal and lower consumption of ZVI.

关键词： groundwater permeable reactive barrier zero valent iron PHT3D denitrification