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Horticultural Plant Journal 2021年第1期7卷 49-58页

作者： Xiaoyun Wang Wencong shi Jilei Wang Zheng Gao Shuang Li Ningxin Wang qinghua shi College of Horticulture Science and Engineering Shandong Agricultural UniversityTai’an 271018China College of Life Sciences Shandong Agricultural UniversityTai’an 271018China College of Plant Protection Shandong Agricultural UniversityTai’an 271018China State Key Laboratory of Crop Biology Shandong Agricultural UniversityTai’an 271018China Shandong Liangjian Bioengineering Co. Ltd.Jinan 271100China

Biofumigation is an environmentally friendly strategy used to control nematodes and plant *** volatile oil of Zanthoxylum bungeanum has high insecticidal and antibacterial ***,it is not known if the seed of *** is a suitable material for biofumigation to control southern root-knot nematodes(SRKN)on tomato,and how it may regulate the soil bacterial community *** used pot experiments in the greenhouse to determine the effects of *** seeds on SRKN,plant growth parameters,soil physicochemical and microbial characteristics.A total of 26 volatile components,including nematicidally active substances,were identified from *** *** seed biofumigation significantly reduced the SRKN population by 88.89%and 81.55%on the 50th and 100th day after transplanting,*** to the control,the total soluble sugar,soluble solids,soluble protein,titratable acid,root activity and the fruit yield per tomato plant increased *** content of soil alkali-hydrolyzed nitrogen,available potassium and the soil enzyme activities were also significantly *** soil bacterial diversity and the co-occurrence network complexity were increased by *** seed *** keystone OTUs in biofumigation soil had potential plant growth-promoting *** function of *** seed increasing tomato production in SRKN-infected soil depends on directly killing SRKN and improving soil *** results indicate that *** seed can be used as both a nematicide and a high quality organic fertilizer in tomato production.

关键词： biofumigant Zanthoxylum bungeanum seed root-knot nematode tomato soil bacterial community

Establishment and characterization of a complete set of Triticum durum-Thinopyrum elongatum monosomic addition lines with resistance to Fusarium head blight in wheat

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Journal of Genetics and Genomics 2019年第11期46卷 547-549页

作者： Jing Wang qinghua shi Xianrui Guo Fangpu Han Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022China State Kay Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Durum wheat(Triticum durum Desf.,2n=28,AABB),which is mainly used to make pasta,is the second cultivated wheat variety ***,the durum wheat production is severely affected by a number of diseases,such as Fusarium head blight(FHB)and stem rust(Klindworth et al.,2017;Zhao et al.,2018).Thinopyrum elongatum(2n=4x=28),a close relative of wheat.

关键词： Establishment characterization Thinopyrum

Effect of Different Rootstocks on the Salt Stress Tolerance in Watermelon Seedlings

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Horticultural Plant Journal 2018年第6期4卷 239-249页

作者： YAN Yanyan WANG Shuoshuo WEI Min GONG Biao shi qinghua State Key Laboratory of Crop Biology Ministry of Agriculture Key Laboratory of Horticultural Crop Biology and Germplasm Creation in Huang-Huai Region College of Horticulture Science and Engineering Shandong Agricultural University

The selection of salt-tolerant watermelon rootstocks requires the evaluation of its underlying mechanism. The present study investigated the effect of applying 200 mmol·L-1 NaCl on various physiological and biochemical parameters of Citrullus lanatus ‘Jingxin No.2'(abbreviated as W) seedlings grafted onto W, Cucurbita moschata ‘Quanneng Tiejia'(P1), ‘Kaijia No.1'(P2), and Lagenaria siceraria ‘Hanzhen No.3'(G), which were grown hydroponically. All the measured growth parameters were significantly inhibited by salt stress, with W/P2 exhibiting superior growth. Salt stress increased Na+uptake, which in turn disrupted K+/Na+homeostasis in all grafting combinations. The photosynthetic capacity, chlorophyll concentration, and photochemical efficiency of photosystem II of all grafts significantly decreased with salt stress. However, W grafted onto G and P2 showed higher resistance than W grafted onto W and P1. Electrolyte leakage(EL) superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase activity were significantly affected by both grafting and salt stress. Minimum EL was observed in W/P2, which also exhibited superior antioxidative capacity. Physiological and biochemical assessment indicated that W grafted onto the P2 rootstock displayed the greatest salt tolerance. Plants grafted onto salt-resistant P2 accumulated less Na+and reactive oxygen species(ROS) and exhibited superior growth,photosynthesis, and ROS-scavenging capacity compared to those grafted onto the other rootstocks.

关键词： watermelon rootstock grafting salt stress physiological response

Genome-wide characterization of MATE family members in Cucumis melo *** their expression profiles in response to abiotic and biotic stress

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Horticultural Plant Journal 2022年第4期8卷 474-488页

作者： Shuoshuo Wang Kun Chen Jiayu Zhang Jianquan Wang Haosen Li Xiaoyu Yang qinghua shi College of Horticulture Science and Engineering Shandong Agricultural UniversityTai’anShandong 271018China State Key Laboratory of Crop Biology Shandong Agricultural UniversityTai’anShandong 271018China Institute of Vegetables Shangqiu Academy of Agriculture and Forestry SciencesShangqiuHenan 476002China

The multidrug and toxic compound extrusion(MATE) family plays pivotal roles in the detoxification process in plants, while no information has been provided for this gene family in melon(Cucumis melo L.) thus far, limiting our understanding of its functions in melon acclimation to stressful environments. In this study, a total of 39 MATEs(CmMATE1–CmMATE39) were observed in the melon genome;these were unevenly distributed in all chromosomes, with the most on Chromosome 1. Based on their orthologous relationship with those from Arabidopsis, rice, and sorghum, melon MATEs were clustered into three subfamilies of Clades Ⅰ, Ⅱ, and Ⅲ, wherein 23, 9, and 7 members were included, *** exon number was observed in CmMATEs, and the most were harbored by CmMATE8. Gene ontology(GO) term and cis-regulatory element(CRE) analyses pointed to the potential roles of CmMATEs in both the regulation of melon development and acclimation to various abiotic and biotic stressors. The RNA-seq and qRT-PCR(quantitative real-time PCR) results demonstrated that under normal growth conditions, CmMATEs were expressed in a tissue-and development-specific manner, while their abundance apparently varied in a stress-dependent manner when melon plants were exposed to unfavorable environmental conditions. Altogether, these observations could expand our knowledge about the plant MATE family and benefit functional genomics analysis for CmMATEs in the future.

关键词： Melon MATE Orthologous relationship GO term Expression Stress

Current understanding of ovarian aging

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Science China(Life Sciences) 2012年第8期55卷 659-669页

作者： LI Qian GENG XiaoDan ZHENG Wei TANG Jie XU Bo shi qinghua Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of ChinaHefei 230027China

The reproductive system of human female exhibits a much faster rate of aging than other body *** aging is thought to be dominated by a gradual decreasing numbers of follicles,coinciding with diminished quality of *** is the final step in the process of ovarian *** review focuses on the mechanisms underlying the ovarian aging involving a poor complement of follicles at birth and a high rate of attrition each month,as well as the alternated endocrine *** also discuss the possible causative factors that contribute to ovarian aging,e.g.,genetic factors,accumulation of irreparable damage of microenvironment,pathological effect and other *** appropriate and reliable methods to assess ovarian aging,such as quantification of follicles,endocrine measurement and genetic testing have also been *** knowledge of the ovarian aging mechanisms may improve the prevention of premature ovarian failure.

关键词： ovarian aging menopause genetic factors microenvironment pathology assessment

Characterization and Genome Changes of New Amphiploids from Wheat Wide Hybridization

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Journal of Genetics and Genomics 2015年第8期42卷 459-461页

作者： Xiang Guo qinghua shi Jing Wang Yanlin Hou Yuhai Wang Fangpu Han State Kay Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences University of Chinese Academy of Sciences

The introduction of alien genes or chromosome fragments from wild related species into wheat （Triticum spp.） has been considered valuable for wheat breeding （Valkoun, 2001）. Several relatives, such as Secale cereale （Gupta and Shepherd, 1993; Ma et al., 2000）, Thinopyrum elongatum （Liu et al., 2008）, Th. intermedium （Cao et al., 2014） and Th. ponticum （Li et al., 2008） showed distinct applications of introgression of interesting genes or traits into wheat. However, there are still some challenges in using these genetic resources. The introgressed alien fragments often bring genes with potentially negative impacts on the traits. In this letter, we mainly focus on multi-strategies for synthesis of polyploids between wheat and rye, Th. elongatum, Th. intermedium and Th. ponticum. We have successfully selected different novel ploidy triticale and trititrigia lines which carried excellent disease-resistance genes and could provide essential genetic resources for wheat breeding.

关键词： Th gene GISH

Site-specific transfer of chromosomal segments and genes in wheat engineered chromosomes

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Journal of Genetics and Genomics 2017年第11期44卷 531-539页

作者： Jing Yuan qinghua shi Xiang Guo Yalin Liu Handong Su Xianrui Guo Zhenling Lv Fangpu Han State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Recently, engineered minichromosomes have been produced using a telomere-mediated truncation technique in some plants. However, the study on transferring genes to minichromosomes is very ***, telomere-mediated truncation was successfully performed in common wheat（Triticum aestivum）to generate stable truncated chromosomes accompanied by a relatively high frequency of chromosomal rearrangements. After the cross between transgenic parents, a promoter-less DsRed gene in a chromosome from one parent was transferred to another chromosome from the other parent at the site behind a maize ubiquitin promoter via the Cre/lox system. DsRed transcripts and red fluorescent proteins were detected in the recombinant plants. In one such seedling, transgenic signals were detected at the centric terminus of chromosome 4D and the distal terminus of chromosome 3A. Clear translocations could be detected at the transgenic loci of these two chromosomes. Intriguingly, signals of centric-specific sequences were co-localized with the translocated D-group chromosomal segment in the terminal region of chromosome 3A. Our results indicate that the Cre/lox system induces the gene swapping to the target chromosome and non-homologous chromosomal recombination simultaneously. These approaches could offer a platform to transfer large DNA fragments or even terminal chromosomal segments to other chromosomes of the natural genome.

关键词： Telomere-mediated truncation Engineered chromosome Crellox system Chromosomal recombination Chromosome translocation Triticum aestivum

The Effect of Composite Flexures on Aeroelastic Stability of a Hingeless Rotor Blade

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航空制造技术 2007年第Z1期50卷 128-133页

作者： shi qinghua Beijing Aeronautical Manufacturing Technology Research Institute

The effects of ply orientation angle of composite flexures on stability of hingeless rotor blade system are *** composite hingeless rotor blade system is simplified as a hub,a flap flexure and a lag *** bearing and main *** kinematics formulations are inferred by employing the moderate deflection beam *** shear deformation and warping related to torsion are *** quasi-steady strip theory with dynamic inflow effects is applied to obtain the aerodynamic loads acting on the *** on *** set of finite element formulations of a hingeless rotor blade system is worked *** numerical results show that the ply angle of the composite flexures has great effects on the aeroelastic stability of rotor blade.

关键词： Aeroelastic Stability Blade Composite Flexure beam

Integrated transcriptomics and metabolomics analyses reveal the molecular mechanisms of red-light on carotenoids biosynthesis in tomato fruit

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Food Quality and Safety 2022年第1期6卷 56-67页

作者： Jun Tang Yan Li Zhongliang Liu Min Wei qinghua shi Fengjuan Yang College of Horticultural Science and Engineering Shandong Agricultural UniversityTai'anChina Scientific Observing and Experimental Station of Environment Controlled Agricuitural Engineering in Huang-Huai-Hai RegionMinistry of AgricultureTaianChina Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production Tai'anChina State Key Laboratory of Crop Biology Shandong Agricultural UniversityTai'anChina Taian Academy of Agricultural Sciences Tai'anChina

Carotenoids are the main pigments responsible for the coloration and account for the major antioxidant activity of tomato(Solanum lycopersicum L.)*** increments in total carotenoids and lycopene levels were observed in tomato fruit illuminated by red light relative to white light in previous studies,but the mechanism of carotenoid biosynthesis regulated by red light is stll *** the present study,the influence of red light on carotenoid biosynthesis in postharvest tomato fruit was conducted using targeted metabolomics and transcriptomic methods.A total of 25 differentially accumulated carotenoids and 1939 differentially expressed genes were isolated and *** results illustrated that the contents of phytoene and lycopene were considerably higher in fruit treated with red light than those with white light at 12 *** differentially expressed genes are mainly enriched in plant hormone signal transduction,photosynthesis,secondary metabolite biosynthesis,and plant circadian ***,from the results of coexpression network analysis,15 transcription factors from red light-treated fruit were screened;among these,transcription factors of SIERF4,SlbHLH93 and SllAA29,which are involved in signal transduction of light and hormones,respectively,may also play important roles in carotenoid biosynthesis regulated by red light in tomato *** is concluded that red light enhanced carotenoid biosynthesis in postharvest tomato fruit and the mechanisms of enhanced carotenoid biosynthesis were not only associated with the direct regulation by red light signalling,but also with the indirect regulation by hormonal signalling.

关键词： Red light tomato carotenoids targeted metabolomics coexpression analysis