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Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression

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Journal of Molecular Cell Biology 2022年第1期14卷 32-46页

作者： Yuwei Li Chencheng Yang Huishan Wang Ling Zhao qinghua kong Yu Cang Shuhua Zhao Longbao Lv Yan Li Bingyu Mao Pengcheng Ma State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Kunming College of Life Science University of Chinese Academy of SciencesKunming 650203China Experimental Animal Center Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Kunming Institute of Botany Chinese Academy of SciencesKunming 650223China Department of Urology the Affiliated Hospital of Yunnan UniversityKunming 650021China First Affiliated Hospital of Kunming Medical University Kunming 650032China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China

Sonic hedgehog (Shh) signaling is essential for the proliferation of cerebellar granule neuron progenitors (CGNPs), and its misregulation is linked to various disorders, including cerebellar cancer medulloblastoma (MB). During vertebrate neural development, RNF220, a ubiquitin E3 ligase, is involved in spinal cord patterning by modulating the subcellular location of glioma-associated oncogene homologs (Glis) through ubiquitination. RNF220 is also required for full activation of Shh signaling during cerebellum development in an epigenetic manner through targeting embryonic ectoderm development. ZC4H2 was reported to be involved in spinal cord patterning by acting as an RNF220 stabilizer. Here, we provided evidence to show that ZC4H2 is also required for full activation of Shh signaling in CGNP and MB progression by stabilizing RNF220. In addition, we found that the ubiquitin E3 ligase RING finger LIM domain-binding protein (RLIM) is responsible for ZC4H2 stabilization via direct ubiquitination, through which RNF220 is also thus stabilized. RLIM is a direct target of Shh signaling and is also required for full activation of Shh signaling in CGNP and MB cell proliferation. We further provided clinical evidence to show that the RLIM‒ZC4H2‒RNF220 cascade is involved in Shh-group MB progression. Disease-causative human RLIM and ZC4H2 mutations affect their interaction and regulation. Therefore, our study sheds light on the regulation of Shh signaling during cerebellar development and MB progression and provides insights into neural disorders caused by RLIM or ZC4H2 mutations.

关键词： ZC4H2 RLIM RNF220 Shh signaling cerebellum medulloblastoma(MB)

ZC4H2 stabilizes RNF220 to pattern ventral spinal cord through modulating Shh/Gli signaling

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Journal of Molecular Cell Biology 2020年第5期12卷 337-344页

作者： Pengcheng Ma Ning-Ning Song Xiaoning Cheng Liang Zhu Qiong Zhang Long long Zhang Xiangcai Yang Huishan Wang qinghua kong Deli Shi Yu-Qiang Ding Bingyu Mao State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institute of Brain SciencesFudan UniversityShanghai 200032China Affiliated Hospital of Guangdong Medical University Zhanjiang 524001China Kunming College of Life Science University of Chinese Academy of SciencesKunming 650223China Key Laboratory of Arrhythmias Ministry of Education of ChinaEast Hospitaland Department of Anatomy and NeurobiologyCollaborative Innovation Center for Brain ScienceTongji University School of MedicineShanghai 200092China State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650203China Department of Laboratory Animal Science Fundan UniversityShanghai 200032China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China Present address:Department of Clinical Laboratory The Affiliated Hospital of KMUSTMedical FacultyKunming University of Science and TechnologyKunming 650032China

ZC4H2 encodes a C4H2 type zinc-finger nuclear factor,the mutation of which has been associated with disorders with various clinical phenotypes in human,including developmental delay,intellectual disability and ***4H2 has been suggested to regulate spinal cord patterning in zebrafish as a co-factor for RNF220,an ubiquitin E3 ligase involved in Gli *** we showed that ZC4H2 and RNF220 knockout animals phenocopy each other in spinal patterning in both mouse and zebrafish,with mispatterned progenitor and neuronal domains in the ventral spinal *** showed evidence that ZC4H2 is required for the stability of RNF220 and also proper Gli ubiquitination and signaling in *** data provides new insights into the possible etiology of the neurodevelopmental impairments observed in ZC4H2-associated syndromes.

关键词： ZC4H2 RNF220 spinal cord patterning Gli signaling

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区域物流与区域经济发展关系研究

物流技术 2013年第9期32卷 268-270页

作者：孔庆华西安航空职业技术学院陕西西安710089

区域物流与区域经济两者间发展为互动关系,在Y市区域物流产值、投入指标与区域经济指标相关性分析的基础上对该地区区域物流与区域经济的发展关系进行了分析与验证,并提出了相应建议。

关键词：区域物流区域经济互动关系

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论肖伯纳的散文作品

外国文学研究 2002年第3期24卷 53-56页

作者：孔庆华青岛大学师范学院外语系 266071

本文结合肖伯纳的散文作品从宗教信仰、教育制度、学术研究、社会时尚等领域分析了作者的散文对英国社会的批判 ,同时又对肖伯纳散文的幽默、比喻及结尾等主要艺术特色进行了分析 ,从而肯定了肖伯纳散文在思想、艺术及认识方面的重要价值。

关键词：散文作品肖伯纳艺术特色作者幽默社会时尚结尾宗教信仰批判价值

RACK1 is essential for adipocyte differentiation through regulating β-Catenin signaling

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RACK1 is essential for adipocyte differentiation through reg...

脂质组学与脂蛋白的新功能——第十二届全国脂质与脂蛋白学术会议

作者： qinghua kong Lan Gao Yan Li Wenyong Xiong State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of Sciences

Objectives:Adipose tissue plays critical roles in maintaining whole body energy *** healthy state,adipocyte differentiation is precisely regulated by many signaling pathways,and it is of great interests to identify new regulators in the ***1 has been identified as an adaptor protein in multiple intracellular signal transduction ***,its function in adipogenesis has not been reported to ***,we explored the

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利率市场化、股权结构与银行风险承担行为

金融发展研究 2017年第12期 54-59页

作者：陈敏孔庆华山东理工大学经济学院山东淄博255012

股权结构会改变银行风险承担行为,而利率市场化则会影响二者关联的强度。本文基于2006—2015年我国上市商业银行面板数据,运用GMM估计方法,从股权集中度、股权属性、股权制衡度三个维度考察了股权结构对商业银行风险承担行为的影响,并... 详细信息

股权结构会改变银行风险承担行为,而利率市场化则会影响二者关联的强度。本文基于2006—2015年我国上市商业银行面板数据,运用GMM估计方法,从股权集中度、股权属性、股权制衡度三个维度考察了股权结构对商业银行风险承担行为的影响,并在此基础上分析了利率市场化对二者关系的调节作用。结果表明,股权集中度与银行风险承担呈显著正向关系。股权集中度越高,其承担的风险越高;股权制衡度与银行风险承担水平呈显著负相关。中小股东对大股东的制衡能够削弱银行风险承担;国家控股和具有政府背景的银行与其他银行相比,风险承担意愿更强;利率市场化有助于弱化股权集中度和股权性质对商业银行风险承担行为的正向作用,同时强化了股权制衡能力对商业银行风险承担行为的负向作用。基于此,本文建议不断优化银行股权结构、进一步推进利率市场化,以更合理地管理商业银行风险承担行为。

关键词：股权结构利率市场化商业银行风险承担

Analysis of Nitrogen Metabolism Related Enzymes and Related Physiological Indexes of Main Foxtail Millet Varieties Developed in North China

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Agricultural Biotechnology 2019年第6期8卷 9-14页

作者： qinghua kong Erying CHEN Shuting YU Yanbing YANG Ling QIN Feifei LI Yanan GUAN Shandong Normal University Jinan250014China Institute of Crop Sciences Shandong Academy of Agricultural SciencesJinan 250100China Qingdao Ruibo Xingke Biotechnology Co. LtdQingdao266011China

[Objectives] This study was conducted to clarify the evolution characteristics of foxtail millet varieties in different ages and provide a basis for the breeding of new varieties. [Methods] A field experiment was carried out on 20 main foxtail millet varieties promoted in North China developed from the 1980 s to 2000 s. The physiological and biochemical indexes of different foxtail millet varieties in four ages were compared, including chlorophyll content, soluble protein content, glutamine synthetase(GS) activity and glutamate synthase(GOGAT) activity, and the correlation between enzyme activity and yield was analyzed. [Results] The chlorophyll SPAD values of the flag leaf and functional leaves of foxtail millet varieties decreased with the filling process. The SPAD values of the flag leaf, top second leaf and top third leaf were higher in the varieties developed in the 1990 s and 2000 s than those in the 1980 s and 2010 s. The activity of glutamine synthetase(GS) and glutamate synthase(GOGAT) showed a single-peak curve in different foxtail millet varieties developed in the recent 30 years, and the peaks of the two were at 7 d and at 7 or 14 d, respectively. The activity of GS and GOGAT increased with the breeding age. In the period from 7 d after anthesis to the mature period, the decreases in the soluble protein content followed an order of 2010 s, 2000 s, 1990 s and 1980 s from small to large, indicating that the degradation rate of various enzyme sources and metabolic regulators in foxtail millet plants decreased during the improvement process. At 35 d after anthesis, the correlation coefficient between GS activity and yield was-0.247, that is, there was a negative correlation with yield. And there was a significant positive correlation between GOGAT activity and yield, and the correlation coefficient was as high as 0.455 at 7 d after anthesis. [Conclusions] Changes in the GS activity, GOGAT activity and soluble protein content in the flag leaf of foxtail mille

关键词： North China Foxtail millet Glutamine synthetase Glutamate synthase Age

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面向语义的制造服务构建技术

同济大学学报（自然科学版） 2012年第1期40卷 102-108页

作者：吴晶晶孔庆华徐克林同济大学机械工程学院上海201804

为了支持网络化协同制造过程中制造服务的匹配、选择和组合,同时提高服务的查全率和查准率,提出了基于语义的制造服务形成方法.首先,从制造服务的基本信息、服务类别、功能、资源构成、关联制造任务、访问、状态及服务质量等方面对制造... 详细信息

为了支持网络化协同制造过程中制造服务的匹配、选择和组合,同时提高服务的查全率和查准率,提出了基于语义的制造服务形成方法.首先,从制造服务的基本信息、服务类别、功能、资源构成、关联制造任务、访问、状态及服务质量等方面对制造服务进行了形式化定义和案例说明.其次,利用XML文档对标准OWL-S(Web服务本体语言)进行了扩展以传达服务的动态信息.然后,采用扩展后的OWL-S对制造服务模型进行了描述实现.最后,给出了协同制造平台原型与运行流程,通过某纺织机械制造企业的生产实例进行了原型系统的验证和应用.

关键词：面向服务架构制造服务形式化定义语义描述

22MnB5钢板不等温拉深成形的影响因素研究

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同济大学学报（自然科学版） 2011年第4期39卷 567-571页

作者：林建平孔庆华徐洲闵峻英同济大学机械工程学院上海201804

建立了高温下方盒形件不等温拉深成形的有限元模型.利用正交试验,研究热冲压过程中模具温度对22MnB5钢板的不等温拉深成形能力的影响,并分析零件几何参数对22MnB5钢板的不等温成形能力的影响.结果表明,模具温度越高,22MnB5钢板的成形能... 详细信息

建立了高温下方盒形件不等温拉深成形的有限元模型.利用正交试验,研究热冲压过程中模具温度对22MnB5钢板的不等温拉深成形能力的影响,并分析零件几何参数对22MnB5钢板的不等温成形能力的影响.结果表明,模具温度越高,22MnB5钢板的成形能力越好;其中凹模温度对成形能力的影响最大,压边圈影响次之,凸模温度影响不大.方盒形件的凸、凹模圆角半径越大,22MnB5钢板在高温下不等温拉深成形能力越好;转角半径越大,转角区域越不易起皱.

关键词：不等温拉深方盒形件有限元模拟成形能力