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Ultrasound-assisted isolation:A new method to isolate stromal vascular fraction

Chinese Journal of Plastic and Reconstructive Surgery 2024年第1期6卷 8-15页

作者： Yiming Gao Xiaojie Zhang Poh-Ching Tan Yun Xie Peiqi Zhang Tianyu Zhang qingfeng Li Shuangbai Zhou Department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China Department of Burns and Plastic Surgery Shanghai Changzheng HospitalShanghai200003China College of Life Sciences Shanghai Normal UniversityShanghai200233China

Background:The stromal vascular fraction(SVF),a cluster of stem and progenitor cells isolated from adipose tissue,holds significant promise for application in regenerative ***,the existing methods for SVF isolation are time-consuming and ***,in this study,we explored a new method of SVF extrac-tion-ultrasound-assisted SVF isolation(USASI)-and compared the viability and characteristics of SVF isolated using different ***:SVF extraction methods using different combinations of ultrasound power,ultrasound time,collagenase dosage,and collagenase digestion time were compared with those of the control group(collagenase digestion method).The cell yield and vitality of the SVF were evaluated via cell counting and trypan blue *** cell components and immunophenotypes of freshly isolated SVF were analyzed using flow *** prolifer-ative capacity and differentiation potential of the SVF were also ***:Ultrasonication at 95 w-20 kHz for 30 s followed by digestion with 0.15%collagenase for 30 min was identified as the most suitable parameter for the USASI method in isolating SVF,as recommended based on the evaluation of various tested *** USASI method significantly reduced the collagenase dosage and shortened the digestion *** to the collagenase digestion method,the USASI method had a higher cell yield and cell viability,with no adverse effects on cell components,proliferative capacity,or multipotential differentiation ***:with reduced processing time,lower collagenase dosage,and increased cell yield without impairing the viability and characteristics of SVF,USASI holds the potential to emerge as a time-saving and cost-effective method for future clinical applications.

关键词： Stromal vascular fractions SVF isolation Mechanical force Ultrasound

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Utilization of Basalt Saw Mud as a Spherical Porous Functional Aggregate for the Preparation of Ordinary Structure Concrete

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Journal of wuhan University of Technology(Materials Science) 2024年第2期39卷 364-375页

作者：周永祥关青锋 LEng Faguang w.ng Jing LI Tianjun Faculty of Architecture Civil and Transportation EngineeringBeijing University of TechnologyBeijing 100124China Key Laboratory of Urban Security and Disaster Engineering of Ministry of Education Beijing University of TechnologyBeijing 100012China School of Materials Science and Engineering Southeast UniversityNanjing 211189China Institute of Building Materials China Academy of Building ResearchBeijing 100013China State Key Laboratory of Building Safety and Built Environment Beijing 100013China Zhejiang Chinavigor E.P.Technology Co. Ltd.Zhejiang 315043China

To promote the production and application of artificial aggregates,save natural sand resources and protect the ecological environment,we evaluated the feasibility of using spherical porous functional aggregates(SPFAs) formed by basalt saw mud under autoclave curing in ordinary structural *** our work,two types of prewetted functional aggregates were taken as replacements for natural aggregates with different volume substitution rates(0%,5%,10%,15%,20%,25%,and 30%) in the preparation of ordinary structural concrete with water-to-binder ratios(w/B) of 0.48 and *** effects of the functional aggregate properties and content,w/B,and curing age on the fluidity,density,mechanical properties and autogenous shrinkage of ordinary concrete were *** experimental results showed that the density of concrete declined at a rate of not more than 5%,and the 28 d compressive strength could reach 31.0-68.2 *** w/B,long curing age and high-quality functional aggregates were conducive to enhancing the mechanical properties of SPFAs *** the rolling effects,SPFAs can optimize the particle gradation of aggregate systems and improve the fluidity of concrete,and the water stored inside SPFAs provides an internal curing effect,which prolongs the cement hydration process and considerably reduces the autogenous shrinkage of *** exhibits high strength and high density,as well as being more cost-effective and ecological,and is expected to be widely employed in ordinary structural concrete.

关键词： lightweight concrete civil concrete building basalt saw mud fly ash internal curing environmentally friendly

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运动疗法联合帕立骨化醇治疗MHD伴继发性甲状旁腺功能亢进患者的效果及对其FGF23及Klotho蛋白水平的影响

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反射疗法与康复医学 2024年第3期5卷 61-64页

作者：黄庆丰建始县第三医院血液透析室湖北建始445300

目的探讨运动疗法联合帕立骨化醇治疗维持性血液透析(MHD)伴继发性甲状旁腺功能亢进(SHPT)患者的效果及对其成纤维细胞生长因子23(FGF23)及Klotho蛋白水平的影响.方法选取2022年2月—2023年2月建始县第三医院收治的94例MHD合并SHPT患者... 详细信息

目的探讨运动疗法联合帕立骨化醇治疗维持性血液透析(MHD)伴继发性甲状旁腺功能亢进(SHPT)患者的效果及对其成纤维细胞生长因子23(FGF23)及Klotho蛋白水平的影响.方法选取2022年2月—2023年2月建始县第三医院收治的94例MHD合并SHPT患者为研究对象,按照随机数字表法将其分为对照组(n=47)及观察组(n=47).对照组采用帕立骨化醇治疗,观察组在此基础上采用运动疗法治疗.比较两组患者的钙磷代谢情况、血清学相关指标水平及生活质量.结果治疗后,观察组的血磷水平低于对照组,血钙水平高于对照组,组间差异有统计学意义(Png/L、(254.43±58.61)ng/L,均低于对照组的(114.26±11.54)ng/L、(324.54±54.14)ng/L,组间差异有统计学意义(P<0.05).治疗后,观察组的肾病相关生存质量量表评分为(69.23±9.36)分,高于对照组的(61.35±8.26)分,差异有统计学意义(P<0.05).结论采用运动疗法联合帕立骨化醇治疗MHD伴SHPT患者,可调控其iPTH、FGF23及Klotho蛋白,有效改善钙磷代谢情况,提高生活质量.

关键词：继发性甲状旁腺功能亢进帕立骨化醇运动疗法维持性血液透析成纤维细胞生长因子23

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Multiscale simulation of the urban wind environment under typhoon weather conditions

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建筑模拟(英文) 2023年第9期16卷 1713-1734页

作者： Zihan Zhao Yiqing Xiao Chao Li P.w.Chan Gang Hu qingfeng Zhou School of Construction Engineering Shenzhen PolytechnicShenzhenChina School of Civil and Environmental Engineering Harbin Institute of Technology ShenzhenShenzhenChina Hong Kong Observatory KowloonHong KongChina Shenzhen Urban Planning&Land Resources Research Center ShenzhenGuangdong 518055China

Accurate simulations of planetary boundary layer(PBL)winds in urban areas require combining meteorological knowledge and fine-grained geometrical *** fluid dynamics(CFD)is widely used to assess pedestrian wind comfort and wind disasters in planning resilient ***,the CFD-predicted PBL is highly affected by the inflow *** profiles under extreme weather conditions,such as tropical cyclones,can hardly be determined,and associated uniform logarithmic or power law expressions have not been *** this study,urban wind flow over mountainous terrain was simulated using a one-way nested simulation approach between mesoscale and microscale *** inflow wind speed,turbulence scalars,and potential temperature in the CFD code are sustained by the numerical weather prediction(NwP)*** considering typhoon weather conditions were examined to improve the numerical accuracy in determining mesoscale typhoon structures and pedestrian-level wind *** numerical errors were quantified in mesoscale and microscale formulations.A new tendency assimilation was proposed by incorporating local-scale observations into the CFD *** approach entailed empirical mode decomposition to quantify the mean wind speed differences between the observations and NwP results,which were then extrapolated to NwP-CFD nested interfaces via multiplication by the spatial correlation *** numerical performance was validated against both on-site observations for meteorological purposes and wind profiles retrieved from the experimental LiDAR of the landfalling typhoon *** simulated wind field exhibited an increased accuracy in the local urban *** specifically,the index of agreement in wind speeds was improved from 0.28 to 0.72,and the mean absolute errors were reduced from 5.46 m/s to 1.89 m/s.

关键词： NwP-CFD urban wind flow data assimilation typhoon disaster resilient cities

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Tungsten Isotopic Constraints on the Nature of Earth's Accreting Materials

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Acta Geologica Sinica(English Edition) 2022年第4期96卷 1213-1220页

作者： MEI qingfeng YAng Jinhui State Key Laboratory of Lithospheric Evolution Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China

Tungsten isotope is a powerful tracer for the Earth’s accreting materials because of the distinct w isotope compositions of the non-carbonaceous meteorites and carbonaceous *** better understand the evolution of the early Earth,here we calculated the expectedμ^(183)w of the bulk silicate Earth for different assumed compositions of the proto-Earth’s mantle,the Moon-forming giant impactor,and the late veneer using a Monte Carlo *** result shows that the proto-Earth likely has a non-carbonaceous composition,while the carbonaceous chondrite-like materials were delivered to the Earth at the late stages of *** predicted difference inμ^(183)w values between the bulk silicate Earth and the non-carbonaceous meteorites of the scenarios assuming a pure carbonaceous composition for the giant impactor is slightly bigger than that of the scenarios assuming either a pure non-carbonaceous or a mixed carbonaceous-non-carbonaceous composition for the giant impactor(~5 ppm versus~2 ppm).The ancient mantle reservoir that partially lacks the late veneer with carbonaceous composition should have a negativeμ^(183)w value(from-3 to 0).Uncertainties introduced by the cosmogenic effects and mass-independent fractionation should be concerned during the high precision measurement ofμ183w for meteorites and ancient terrestrial samples in further work.

关键词： tungsten isotope nucleosynthetic anomalies Moon-forming giant impact late veneer

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Influence of the symmetry energy on the balance energy of the directed flow

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Science China(Physics,Mechanics & Astronomy) 2012年第2期55卷 252-259页

作者： GUO ChenChen w.ng YongJia LI qingfeng TRAUTMANN w LIU Ling wU LiJuan School of Science Huzhou Teachers CollegeHuzhou 313000China College of Physics Science&Technology Shenyang Normal UniversityShenyang 110034China School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000China GSI Helmholtzzentrum fuer Schwerionenforschung GmbH Darmstadt D-64291Germany

The influence of the density-dependent symmetry energy on the balance energy （Ebal） of directed flow from heavy ion collisions （HICs） at incident energies covered by INDRA and MSU experiments is studied, using the updated version of the ultra- relativistic quantum molecular dynamics （UrQMD） model, especially adapted to low-energy heavy ion collisions （HICs）. Four mass-symmetric reactions with total mass numbers between 192 and 394 are chosen for investigating the influence of the symmetry energy on the system-mass dependence of Eual. The results show that the uncertainty in the density dependence of the symmetry potential causes changes of Ebal of the order of several MeV, depending on the type of particle considered. The Ebal of neutrons from HICs is particularly sensitive to the density dependence of the symmetry potential energy, while the system-mass dependence of EbaI of Z = 1 particles is not.

关键词： symmetry energy directed flow balance energy

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抗缪勒管激素诊断多囊卵巢综合征的年龄特异性阈值探讨及应用

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国际检验医学杂志 2024年第10期45卷 1248-1252页

作者：周庆丰曹敏上海市松江区中心医院检验科上海201600

目的探讨抗缪勒管激素(AMH)诊断多囊卵巢综合征(PCOS)的年龄特异性阈值及其诊断效能。方法收集该院2 187例14~40岁的月经紊乱的患者样本,并采用免疫荧光方法测定血清AMH水平,符合鹿特丹标准患者为PCOS组,反之为对照组。利用广义加性模... 详细信息

目的探讨抗缪勒管激素(AMH)诊断多囊卵巢综合征(PCOS)的年龄特异性阈值及其诊断效能。方法收集该院2 187例14~40岁的月经紊乱的患者样本,并采用免疫荧光方法测定血清AMH水平,符合鹿特丹标准患者为PCOS组,反之为对照组。利用广义加性模型(GAMs)和受试者工作特征(ROC)曲线下面积(AUC)确立AMH的年龄区间和对应的阈值。评估各年龄区间AMH诊断PCOS的效能,并与试剂厂商提供的阈值进行比较。结果基于GAMs模型,AMH预测PCOS的最佳年龄截断值分别为21、28和35岁。在14~ng/mL;AUC分别为0.97(95%CI:0.93~0.99)、0.97(95%CI:0.95~0.98)、0.98(95%CI:0.95~0.98)、0.99(95%CI:0.98~0.99);灵敏度分别为0.87、0.87、0.91、1.00;特异度分别为0.94、0.93、0.90、0.93。在各年龄组中,PCOS组的AMH水平均高于对照组,差异具有统计学意义(P<0.001)。结论应用AMH的年龄特异性阈值,可以更好地评估AMH在区分所有年龄段PCOS患者中的价值。

关键词：抗缪勒管激素年龄特异性阈值广义加性模型多囊卵巢综合征诊断效能

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利拉鲁肽对高糖诱导的足细胞凋亡的影响及机制

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临床医药实践 2024年第8期33卷 563-568,576页

作者：荣青峰郭娜韩乐山西医科大学第二医院山西太原030001

目的:探讨利拉鲁肽对高糖诱导的足细胞凋亡的影响及机制。方法:将购买的小鼠足细胞(MPC5)分为7组:正常糖(ng)组、高糖(HG)组、甘露醇(M)组、高糖+沉默信息调节因子1(SIRT1)激动剂(HG+RSV)组、高糖+SIRT1激动剂+SIRT1阻断剂(HG+RSV+S)组... 详细信息

目的:探讨利拉鲁肽对高糖诱导的足细胞凋亡的影响及机制。方法:将购买的小鼠足细胞(MPC5)分为7组:正常糖(ng)组、高糖(HG)组、甘露醇(M)组、高糖+沉默信息调节因子1(SIRT1)激动剂(HG+RSV)组、高糖+SIRT1激动剂+SIRT1阻断剂(HG+RSV+S)组、高糖+胰高血糖素样肽-1受体(GLP-1R)激动剂利拉鲁肽(HG+LG)组,高糖+胰高血糖素样肽-1受体(GLP-1R)激动剂+GLP-1R阻断剂(HG+LG+E)组。刺激72 h后,采用流式细胞术检测足细胞凋亡率;共聚焦荧光成像技术检测足细胞骨架蛋白synaptopodin表达水平;免疫印迹检测GLP-1R,SIRT1表达。结果:HG组足细胞凋亡率升高,synaptopodin,SIRT1和GLP-1R表达降低,与ng组比较,差异有统计学意义(P<0.01);HG+RSV组足细胞凋亡率降低,SIRT1表达升高,与HG组比较,差异有统计学意义(P<0.01);而SIRT1特异性拮抗剂则阻断了SIRT1激动剂对足细胞的保护作用;HG+LG组足细胞凋亡率降低,SIRT1和GLP-1R表达升高,与HG组比较差异有统计学意义(P<0.01);而GLP-1R特异性拮抗剂则阻断了GLP激动剂对SIRTI的表达调控作用。结论:高糖可诱导足细胞发生凋亡和损伤,利拉鲁肽可减少高糖诱导的足细胞凋亡,其机制可能与激活GLP-1R/SIRT1信号途径有关。

关键词：足细胞凋亡利拉鲁肽沉默信息调节因子1

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气相色谱-质谱法测定帕瑞昔布钠中3种硫酸酯类基因毒性杂质

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化学分析计量 2024年第8期33卷 56-61,68页

作者：崔萍曹庆丰丁逸梅江苏省药物研究所有限公司南京211816 南京工业大学药学院南京211816

建立气相色谱-质谱法检测帕瑞昔布钠原料药及注射用帕瑞昔布钠中硫酸二甲酯、硫酸二乙酯和硫酸二异丙酯。用Thermo TG-5MS毛细管色谱柱(30 m×0.25 mm,0.25μm)分离,载气为氦气,质谱离子源为电子轰击离子源,采集模式为定时选择性离子监... 详细信息

建立气相色谱-质谱法检测帕瑞昔布钠原料药及注射用帕瑞昔布钠中硫酸二甲酯、硫酸二乙酯和硫酸二异丙酯。用Thermo TG-5MS毛细管色谱柱(30 m×0.25 mm,0.25μm)分离,载气为氦气,质谱离子源为电子轰击离子源,采集模式为定时选择性离子监测。3种硫酸酯质量浓度在各自范围内与色谱峰面积线性关系良好,相关系数均大于0.995,平均回收率为95.60%~107.3%,测定结果的相对标准偏差均小于5%(n=6),检出限为20.50~21.88 ng/mL,定量限为61.50~65.64 ng/mL。该法可用于帕瑞昔布钠原料药及注射用帕瑞昔布钠中3种硫酸酯类基因毒性杂质的检测。

关键词：气相色谱-质谱法帕瑞昔布钠注射用帕瑞昔布钠硫酸酯类基因毒杂质

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Novel voltage signal at proximity-induced superconducting transition temperature in gold nanowires

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Science China(Physics,Mechanics & Astronomy) 2018年第8期61卷 69-74页

作者： Jian w.ng JunXiong Tang ZiQiao w.ng Yi Sun qingfeng Sun Moses H.w.Chan International Center for Quantum Materials School of PhysicsPeking UniversityBeijing 100871China Collaborative Innovation Center of Quantum Matter Beijing 100871China State Key Laboratory of Low-Dimensional Quantum Physics Department of PhysicsTsinghua UniversityBeijing 100084China Department of Physzcs The Pennsylvania State UniversityPennsylvania 16802-6300USA CAS Center for Excellence in Topological Quantum Computation University of Chinese Academy of SciencesBeijing 100190China Department of Physics University of Colorado BoulderCO 80309USA

we observed a novel voltage peak in the proximity-induced superconducting gold(Au) nanowire while cooling the sample through the superconducting transition temperature. The voltage peak turned dip during warming. The voltage peak or dip was found to originate respectively from the emergence or vanishing of the proximity-induced superconductivity in the Au *** amplitude of the voltage signal depends on the temperature scanning rate, and it cannot be detected when the temperature is changed slower than 0.03 K/min. This transient feature suggests the non-equilibrium property of the effect. Ginzburg-Landau model clarified the voltage peak by considering the emergence of Cooper pairs of relatively lower free energy in superconducting w contact and the non-equilibrium diffusion of Cooper pairs and quasiparticles.

关键词： proximity-induced superconductivity Au nanowire non-equilibrium process

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