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Effect of biochar application on rice,wheat,and corn seedlings in hydroponic culture

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Journal of Environmental Sciences 2024年第1期135卷 379-390页

作者： Yingjie Yin Jikai Li Sihang zhu qing Chen Chong Chen Yukui Rui Jianying Shang College of Land Science and Technology China Agricultural UniversityKey Laboratory of Plant-Soil InteractionsMinistry of Educationand Key Laboratory of Arable Land Conservation in North ChinaMinistry of Agriculture and Rural AffairsBeijing 100193China Agricultural Management Institute Ministry of Agriculture and Rural AffairsBeijing 102208China College of Resources and Environmental Sciences China Agricultural UniversityBeijing Key Laboratory of Farmland Soil Pollution Prevention and RemediationBeijing 100193China

In recent years,biochar has attracted considerable attention for soil quality improvement and carbon sequestration due to its unique physicochemical properties.However,the mechanism by which biochar application negatively affects the growth of crop seedlings has not been fully investigated.In this study,a hydroponic experiment was conducted to evaluate the response of rice,wheat,and corn seedlings to biochar application(CK,0 g/L;BC1,0.5 g/L;and BC2,1.0 g/L).Compared with the CK treatment,the BC1 and BC2 treatments decreased the fresh shoot and root weights of rice and corn seedlings(P0.05).For the contents of nutrient elements in seedlings,both BC1 and BC2 treatments hindered the roots from absorbing Fe and Cu and increased the uptake of Ca and Mn.Compared with the CK treatment,the translocation factor(TF)values of Ca,Mn,and Zn were significantly decreased especially in rice seedlings(35.3%-36.8%,68.7%-76.5%,and 29.8%-22.0%,respectively)under the BC1 and BC2 treatments,while only Mn was significantly decreased in wheat and corn seedlings(P<0.05).Transmission electron microscope(TEM)analysis of root cross-sections showed that nano-sized biochar particles(10~23 nm)were found in the root cells under BC2 treatment conditions.Our findings reveal that a large amount of biochar application can reduce nutrient absorption and translocation,and hinder rice,wheat,and corn seedlings,particularly rice seedling,in hydroponic system.

关键词： Biochar Toxicity Seedling growth Hydroponic cultivation

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Day-ahead P2P Energy Sharing Strategy Among Energy Hubs Considering Flexibility of Energy Storage and Loads

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CSEE Journal of Power and Energy Systems 2024年第3期10卷 1105-1118页

作者： Penghua Li Wanxing Sheng qing Duan Jun Liang Cunhao zhu China Electric Power Research Institute Beijing 100192China School of Engineering Cardiff UniversityUK IEEE

Multi-energy systems are one of the key technologies to tackle energy crisis and environmental pollution.An energy hub(EH)is a minimum multi-energy system.Interconnection of multiple EHs through energy routers(ERs)can realize mutual energy assistance.This paper proposes a peer-to-peer(P2P)energy sharing strategy between EHs including ERs in an interconnected system,which is divided into two levels.In the lower level,a method of determining the charging/discharging constraints of energy storage devices is proposed.Based on the Lyapunov optimization method,virtual queues are used to model the energy storage devices and flexible loads in the system.The objective is to minimize the overall operating cost of the interconnected system.In the upper level,a non-cooperative game model is introduced to minimize the cost of purchasing power from other EHs for each EH.A best response-based method is adapted to find the Nash equilibrium.The simulation outcomes demonstrate that application of the proposed strategy can reduce operating costs of an interconnected system and each EH.On basis of a real-world dataset of interconnected EHs,both analytical and numerical results show the effectiveness of the proposed strategy.

关键词： Energy hub energy router Lyapunov optimization non-cooperative game peer to peer virtual queue

In Vivo Voltammetric Imaging of Metal Nanoparticle-Catalyzed Single-Cell Electron Transfer by Fermi Level-Responsive Graphene

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Research 2024年第1期2023卷 271-280页

作者： qing Xia Rui Liu Xueqin Chen Zixuan Chen Jun-Jie zhu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023P.R.China Shenzhen Research Institute of Nanjing University Shenzhen 518000P.R.China

Metal nanomaterials can facilitate microbial extracellular electron transfer(EET)in the electrochemically active biofilm.However,the role of nanomaterials/bacteria interaction in this process is still unclear.Here,we reported the single-cell voltammetric imaging of Shewanella oneidensis MR-1 at the single-cell level to elucidate the metal-enhanced EET mechanism in vivo by the Fermi level-responsive graphene electrode.Quantified oxidation currents of~20 fA were observed from single native cells and gold nanoparticle(AuNP)-coated cells in linear sweep voltammetry analysis.On the contrary,the oxidation potential was reduced by up to 100 mV after AuNP modification.It revealed the mechanism of AuNP-catalyzed direct EET decreasing the oxidation barrier between the outer membrane cytochromes and the electrode.Our method offered a promising strategy to understand the nanomaterials/bacteria interaction and guide the rational construction of EET-related microbial fuel cells.

关键词： Fermi voltammetry Electron

Development and validation of a new prognostic model for patients with acute-on-chronic liver failure in intensive care unit

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World Journal of Gastroenterology 2024年第20期30卷 2657-2676页

作者： Zong-Yi zhu Xiu-Hong Huang Hui-qing Jiang Li Liu Department of Gastroenterology The Second Hospital of Hebei Medical UniversityShijiazhuang 050000Hebei ProvinceChina Department of Gastroenterology Weixian People's HospitalXingtai 054700Hebei ProvinceChina

BACKGROUND Cirrhotic patients with acute-on-chronic liver failure(ACLF)in the intensive care unit(ICU)have a poor but variable prognoses.Accurate prognosis evaluation can guide the rational management of patients with ACLF.However,existing prognostic scores for ACLF in the ICU environment lack sufficient accuracy.AIM To develop a new prognostic model for patients with ACLF in ICU.METHODS Data from 938 ACLF patients in the Medical Information Mart for Intensive Care(MIMIC)database were used to develop a new prognostic model(MIMIC ACLF)for ACLF.Discrimination,calibration and clinical utility of MIMIC ACLF were assessed by area under receiver operating characteristic curve(AUROC),calibration curve and decision curve analysis(DCA),respectively.MIMIC ACLF was then externally validated in a multiple-center cohort,the Electronic Intensive Care Collaborative Research Database and a single-center cohort from the Second Hospital of Hebei Medical University in China.RESULTS The MIMIC ACLF score was determined using nine variables:ln(age)×2.2+ln(white blood cell count)×0.22-ln(mean arterial pressure)×2.7+respiratory failure×0.6+renal failure×0.51+cerebral failure×0.31+ln(total bilirubin)×0.44+ln(internationalized normal ratio)×0.59+ln(serum potassium)×0.59.In MIMIC cohort,the AUROC(0.81/0.79)for MIMIC ACLF for 28/90-day ACLF mortality were significantly greater than those of Chronic Liver Failure Consortium ACLF(0.76/0.74),Model for End-stage Liver Disease(MELD;0.73/0.71)and MELD-Na(0.72/0.70)(all P<0.001).The consistency between actual and predicted 28/90-day survival rates of patients according to MIMIC ACLF score was excellent and superior to that of existing scores.The net benefit of MIMIC ACLF was greater than that achieved using existing scores within the 50%threshold probability.The superior predictive accuracy and clinical utility of MIMIC ACLF were validated in the external cohorts.CONCLUSION We developed and validated a new prognostic model with satisfactory accuracy for cirrhotic patients with ACLF hospitalized in

关键词： Acute-on-chronic liver failure Cirrhosis Risk stratification Prognosis Model Scores

Ultraconformable Integrated Wireless Charging Micro-Supercapacitor Skin

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Nano-Micro Letters 2024年第7期16卷 46-58页

作者： Chang Gao qing You Jiancheng Huang Jingye Sun Xuan Yao Mingqiang zhu Yang Zhao Tao Deng School of Electronic and Information Engineering Beijing Jiaotong UniversityBeijing 100044People’s Republic of China School of Microelectronics Tianjin UniversityTianjin 300072People’s Republic of China Key Laboratory of Cluster Science Ministry of Education of China Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion MaterialsSchool of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing 100081People’s Republic of China

Conformable and wire-less charging energy storage devices play important roles in enabling the fast development of wearable,non-contact soft electronics.However,current wire-less charging power sources are still restricted by limited flexural angles and fragile connection of components,resulting in the failure expression of performance and constraining their fur-ther applications in health monitoring wearables and moveable artificial limbs.Herein,we present an ultracompatible skin-like integrated wireless charging micro-supercapacitor,which building blocks(including electrolyte,electrode and substrate)are all evaporated by liquid precursor.Owing to the infiltration and permeation of the liquid,each part of the integrated device attached firmly with each other,forming a compact and all-in-one configuration.In addition,benefitting from the controllable volume of electrode solution precursor,the electrode thickness is easily regulated varying from 11.7 to 112.5μm.This prepared thin IWC-MSC skin can fit well with curving human body,and could be wireless charged to store electricity into high capacitive micro-supercapacitors(11.39 F cm-3)of the integrated device.We believe this work will shed light on the construction of skin-attachable electronics and irregular sensing microrobots.

关键词： Micro-supercapacitor Electronic skin Supercapacitor skin Wireless charging energy storage device

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High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein

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Journal of Animal Science and Biotechnology 2024年第3期15卷 971-986页

作者： Haorong He Yuanhang Wei Yuqi Chen Xiyu Zhao Xiaoxu Shen qing zhu Huadong Yin Key Laboratory of Livestock and Poultry Multi‑Omics Ministry of Agriculture and Rural AffairsCollege of Animal Science and TechnologySichuan Agricultural UniversityChengduSichuan 611130China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengduSichuan 611130China

Background The reproductive performance of chickens mainly depends on the development of follicles.Abnor-mal follicle development can lead to decreased reproductive performance and even ovarian disease among chick-ens.Chicken is the only non-human animal with a high incidence of spontaneous ovarian cancer.In recent years,the involvement of circRNAs in follicle development and atresia regulation has been confirmed.Results In the present study,we used healthy and atretic chicken follicles for circRNA RNC-seq.The results showed differential expression of circRALGPS2.It was then confirmed that circRALGPS2 can translate into a protein,named cir-cRALGPS2-212aa,which has IRES activity.Next,we found that circRALGPS2-212aa promotes apoptosis and autophagy in chicken granulosa cells by forming a complex with PARP1 and HMGB1.Conclusions Our results revealed that circRALGPS2 can regulate chicken granulosa cell apoptosis and autophagy through the circRALGPS2-212aa/PARP1/HMGB1 axis.

关键词： Apoptosis Autophagy Chicken CircRALGPS2 Follicle atresia PARP1 RNC-seq

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Unresolved Excess Accumulation of Myelin-Derived Cholesterol Contributes to Scar Formation after Spinal Cord Injury

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Research 2024年第1期2023卷 17-32页

作者： Bolin Zheng Yijing He Shuai Yin Xu zhu qing Zhao Huiyi Yang Zhaojie wang Rongrong zhu Liming Cheng Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration Ministry of EducationDepartment of OrthopedicsTongji HospitalSchool of MedicineTongji UniversityShanghai 200092China Frontier Science Center for Stem Cell Research School of Life Science and TechnologyTongji UniversityShanghai 200092China Clinical Center for Brain and Spinal Cord Research Tongji UniversityShanghai 200092China

Spinal cord injury triggers complex pathological cascades,resulting in destructive tissue damage and incomplete tissue repair.Scar formation is generally considered a barrier for regeneration in the central nervous system.However,the intrinsic mechanism of scar formation after spinal cord injury has not been fully elucidated.Here,we report that excess cholesterol accumulates in phagocytes and is inefficiently removed from spinal cord lesions in young adult mice.Interestingly,we observed that excessive cholesterol also accumulates in injured peripheral nerves but is subsequently removed by reverse cholesteroltransport.Meanwhile,preventing reverse cholesterol transport leads to macrophage accumulation and fibrosis in injured peripheral nerves.Furthermore,the neonatal mouse spinal cord lesions are devoid of myelin-derived lipids and can heal without excess cholesterol accumulation.We found that transplantation of myelin into neonatal lesions disrupts healing with excessive cholesterol accumulation,persistent macrophage activation,and fibrosis.Myelin internalization suppresses macrophage apoptosis mediated by CD5L expression,indicating that myelin-derived cholesterol plays a critical role in impaired wound healing.Taken together,our data suggest that the central nervous system lacks an efficient approach for cholesterol clearance,resulting in excessive accumulation of myelin-derived cholesterol,thereby inducing scar formation after injury.

关键词： healing Spinal impaired

TRPC6 Knockout Alleviates Renal Fibrosis through PI3K/AKT/GSK3B Pathway

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Current Medical Science 2024年第3期44卷 589-602页

作者： An-bang SUN Fang-hua LI Lin zhu Xi-xi ZENG Min zhu qing-hua LEI Yan-hong LIAO Department of Anatomy School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Transfusion Medicine Wuhan Hospital of Traditional Chinese and Western MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Nephrology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou450052China Department of Thoracic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China National Basic Medical Teaching Demonstration Center Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Key Laboratory of Neurological Diseases of Ministry of Education Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Objective Renal fibrosis is the ultimate pathway of various forms of acute and chronic kidney damage.Notably,the knockout of transient receptor potential channel 6(TRPC6)has shown promise in alleviating renal fibrosis.However,the regulatory impact of TRPC6 on renal fibrosis remains unclear.Methods In vivo,TRPC6 knockout(TRPC6−/−)mice and age-matched 129 SvEv(WT)mice underwent unilateral renal ischemia-reperfusion(uIR)injury surgery on the left renal pedicle or sham operation.Kidneys and serum were collected on days 7,14,21,and 28 after euthanasia.In vitro,primary tubular epithelial cells(PTECs)were isolated from TRPC6−/−and WT mice,followed by treatment with transforming growth factorβ1(TGFβ1)for 72 h.The anti-fibrotic effect of TRPC6−/−and the underlying mechanisms were assessed through hematoxylin-eosin staining,Masson staining,immunostaining,qRT-PCR,and Western blotting.Results Increased TRPC6 expression was observed in uIR mice and PTECs treated with TGFβ1.TRPC6−/−alleviated renal fibrosis by reducing the expression of fibrotic markers(Col-1,α-SMA,and vimentin),as well as decreasing the apoptosis and inflammation of PTECs during fibrotic progression both in vivo and in vitro.Additionally,we found that the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/glycogen synthase kinase 3 beta(GSK3β)signaling pathway,a pivotal player in renal fibrosis,was down-regulated following TRPC6 deletion.Conclusion These results suggest that the ablation of TRPC6 may mitigate renal fibrosis by inhibiting the apoptosis and inflammation of PTECs through down-regulation of the PI3K/AKT/GSK3βpathway.Targeting TRPC6 could be a novel therapeutic strategy for preventing chronic kidney disease.

关键词： transient receptor potential channel 6 ischemia-reperfusion injury renal fibrosis renal tubular epithelial cells

Metal/covalent organic frameworks for aqueous rechargeable zinc-ion batteries

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Science China Chemistry 2024年第1期67卷 247-259页

作者： Hu Hong Xun Guo Jiaxiong zhu zhuoxi Wu qing Li Chunyi Zhi Department of Materials Science and Engineering City University of Hong KongHong Kong 999077China

Lithium-ion batteries(LIBs)have become one of the most successful energy storage systems due to their high operating voltage,high energy density,and long cycle life.However,with the widespread use of LIBs in recent decades,lithium resources are at risk of being exhausted.Therefore,it is necessary to find a substitute for LIBs to meet the needs of future large-scale energy storage systems.Because of their competitiveness,low cost,and high safety,aqueous rechargeable zinc-ion batteries(ARZIBs)are regarded as promising components in the post-lithium-ion-battery era.Given the tunable composition,ordered porous channels,and controllable structure of metal-organic frameworks(MOFs)and covalent organic frameworks(COFs),these frameworks are viewed as potential materials for developing high-performance ARZIBs.In this review,we focus on the recent developments in the applications of MOF-/COF-based materials in ARZIBs,including in electrode materials,anode modifications,separators,and solid electrolytes.We then focus on the critical factors and optimization techniques of MOF-/COF-based materials that affect the performance of ARZIBs.Finally,we conclude with some projections for the expansion of ARZIBs containing MOF-/COF-based materials.

关键词： metal/covalent organic frameworks aqueous zinc-ion batteries electrode materials anode modifications separators electrolytes

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