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Effects of adenovirus-mediated human cyclooxygenase-2 antisense RNA on the growth of hepatocellular carcinoma

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World Journal of Gastroenterology 2005年第39期11卷 6110-6114页

作者： Xiao-Hu Wang Sheng-Bao Li Qiang Tong Guo-Jian Xie qing-ming wu Department of Gastroenterology Taihe Hospital Yunyang Medical College Shiyan 442000 Hubei Province China

AIM: To investigate the relation between the expression of cyclooxygenase-2 (COX-2) and liver cancer, to construct the recombinant adenovirus encoding human COX-2antisense RNA, and to explore its effects on liver cancer cell ***: We studied the expression of COX-2 in 34cases of hepatocellular carcinoma (HCC) and SMMC7402and SMMC7721 by immunohistochemical *** adenovirus Ad-AShcox-2 was constructed and transfected into human HCC cell lines SMMC7402and SMMC7721, and its effects on COX-2 expression, cell apoptosis and cell cycle were analyzed by flow *** proliferation was determined by colony-forming ***: We observed COX-2 expression in 82.4% of HCC and SMMC7402 cells, but no COX-2 expression in SMMC7721 cells. In addition, recombinant adenovirus encoding antisense COX-2 fragment Ad-AShcox-2 was obtained with the titer of 1.06× 1012 PFU/mL. Ad-AShcox-2 could reduce the expression of COX-2 and enhance the percentage of cells in G1/G0 phase in SMMC7402 cell *** difference of apoptotic index between the Ad-AShcox2 group and control group was statistically significant(tcontrol group= 32.62 and tAd-LacZ= 10.93, P＜0.001) in SMMC7402 but not in SMMC7721. Similarly, colony-forming rates of SMMC7402 and SMMC7721 cell lines, after the transfer of Ad-AShcox-2, were (2.7±0.94)% and(33.6±4.24)%, ***: Reduction in the expression of COX-2 can inhibit COX-2 expressing HCC cells.

关键词： Adenovirus Cyclooxygenase-2 Antisense RNA Hepatocellular carcinoma

RiceDB:A Web-Based Integrated Database for Annotating Rice Microarray

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Rice science 2007年第4期14卷 256-264页

作者： HE Fei SHI qing-yun CHEN ming wu Ping State Key Laboratory of Plant Physiology ＆ Biochemistry College of Life Sciences Zhejiang University Hangzhou 3100584 China

RiceDB, a web-based integrated database to annotate rice microarray in various biological contexts was developed. It is composed of eight modules. RiceMap module archives the process of Affymetrix probe sets mapping to different databases about rice, and aims to the genes represented by a microarray set by retrieving annotation information via the identifier or accession number of every database; RiceGO module indicates the association between a microarray set and gene ontology （GO） categories; RiceKO module is used to annotate a microarray set based on the KEGG biochemical pathways; RiceDO module indicates the information of domain associated with a microarray set; RiceUP module is used to obtain promoter sequences for all genes represented by a microarray set; RiceMR module lists potential microRNA which regulated the genes represented by a microarray set; RiceCD and RiceGF are used to annotate the genes represented by a microarray set in the context of chromosome distribution and rice paralogous family distribution. The results of automatic annotation are mostly consistent with manual annotation. Biological interpretation of the microarray data is quickened by the help of RiceDB.

关键词： Affymetrix microarray annotation Oryza sativa molecular database

Role of gut microbiome in regulating the effectiveness of metformin in reducing colorectal cancer in type 2 diabetes

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World Journal of Clinical Cases 2020年第24期8卷 6213-6228页

作者： Qi-You Huang Fei Yao Chuan-Ren Zhou Xiao-Ying Huang Qiang Wang Hui Long qing-ming wu Institute of Infection Immunology and Tumor MicroenvironmentMedical CollegeWuhan University of Science and TechnologyWuhan 430065Hubei ProvinceChina Department of Gastroenterology Tianyou Affiliated HospitalWuhan University of Science and TechnologyWuhan 430064Hubei ProvinceChina

The prevalence of colorectal cancer (CRC) and type 2 diabetes mellitus (T2DM) isincreasing globally. It is rarely noticed that the incidence of CRC is higher inpatients with T2DM. What needs to be mentioned is that metformin, a commonlyused clinical drug for T2DM, attracts scholars’ attention because of its benefits inlowering the risk of developing CRC. Hence, we try to find the common groundsof initiation of T2DM and CRC and the reason why metformin reduces the risk ofCRC in patients with T2DM. We noticed consistent changes of gut microbiota,such as elevated Bacteroides, Prevotella and Bifidobacterium and depressedFirmicutes and Lactobacillus. Furthermore, many studies in recent years haveproved that the efficacy of metformin, such as improving blood glucose, dependson the gut microbiota. Coincidentally, the progression of CRC is inseparable fromthe contributions of gut microbiota. Therefore, we first proposed the concept ofthe metformin-gut microbiota–CRC (in T2DM) axis to explain the effect ofmetformin in reducing CRC in patients with T2DM. In this review, we elaboratedthe new concept and its potential clinical application value.

关键词： Metformin Colorectal cancer Gut microbiota Type 2 diabetes mellitus

Adenovirus expressing p27^(kip1) suppresses growth of established esophageal carcinoma xenografts

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World Journal of Gastroenterology 2005年第42期11卷 6582-6586页

作者： Wei-Guo Zhang qing-ming wu Jie-Ping Yu Qiang Tong Guo-Jian Xie Xiao-Hu Wang Sheng-Bao Li Digestive Department TaiheHospital Yunyang Medical College Shiyan 442000 HubeiProvince China Digestive Department Renmin Hospital of WuhanUniversity Wuhan 430060 Hubei Province China

AIM： TO investigate the growth suppression of adenovirus expressing p27^kip1 on established esophageal tumors in nude mice. METHODS： Esophageal carcinoma xenografts in nude mice were established by tumor tissue mass transplantation. The successfully constructed recombinant adenoviral vectors carrying p2^kip1 gene （Ad- p27^%kip1） were directly injected into the esophageal tumors in nude mice. Compared to control group, the growth curve of tumor was drawn and the growth inhibition rate of tumor was calculated. The histology of tumors was examined by hematoxylin and eosin （H＆E） staining. The expression of p27^kip1 and survivin was detected in tumors by immunohistochemical technique. RESULTS： The growth of tumors in gene therapy group with Ad-p27^kip1 was obviously suppressed compared to control group （0.42±0.08 g vs 1.17±0.30 g, t=6.39, P〈0.01）, the inhibition rate of tumor growth reached 64.1%. Pathological detection showed that the tumors in nude mice were poorly differentiated esophageal squarnous carcinoma. In addition, the expression of p27^kip1 was increased, while the expression of survivin was decreased in tumors after being transfected with Ad-p27^kip1. CONCLUSION： p27^kip1 gene therapy mediated by adenovirus vector has a significant inhibitory effect on esophageal carcinoma in vivo. Up-regulated p27^kip1 expression and down-regulated survivin expression may be its important mechanisms.

关键词： p27^kip1 gene Esophageal carcinoma

Manganese carbonate stromatolites of the Ediacaran Doushantuo Formation in Chengkou,northern Yangtze Craton,China

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Journal of Palaeogeography 2021年第3期10卷 356-381页

作者： Yi Zhang Jian Li Long Chen Yi Wei Qiang Shi Dong-Ge Wang qing-ming wu Liao-Yuan Song Meng Tian Hong-Wei Kuang Yong-qing Liu Kaarel Mänd Hua-qing Bai Zi-Liang Liu Yu-Chong Wang Da-Wei Qiao Wen-Jun Zhu No.205 Geological Team Chongqing Bureau of Geology and Minerals ExplorationChongqing 402160China Institute of Geology Chinese Academy of Geological SciencesBeijing 100037China Department of Earth and Atmospheric Sciences University of AlbertaEdmontonAlberta T6G 2E8Canada Department of Geology University of TartuTartu 50411Estonia State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of TechnologyChengdu 610059Sichuan ProvinceChina PetroChina Xinjiang Oilfield Company Karamay 834099Xinjiang Uygur Autonomous RegionChina

The origin process of manganese ores remains unsolved *** the origins of stromatolites that contain manganese may be a key to deciphering the sedimentary environments and metallogenic processes of these ***,only a few manganese stromatolites have been discovered and described until *** are well developed in the manganese deposits,located near the top of the Ediacaran Doushantuo Formation in Chengkou area of Chongqing,northern Yangtze Craton,but has not been explicitly studied;and whether they are true stromatolites or Epiphyton microbialites remains *** on field and core observations and thin section microscopy,the characteristics of five types of manganese stromatolites and their growth modes are described in detail in this *** results show that these stromatolites grew in a biostrome in shoal and lagoon environments and were syngenetic with oncolites and oolites on a carbonate ramp behind the *** stromatolites can be categorized into three forms:(1)stratiform;(2)columnar,which includes branched and columnar types;and(3)stratiform-columnar,which is a transitional *** on a criterion that the diameter is less than or greater than 1 mm,columnar stromatolites are further divided into micro-columnar(1 mm)columns,which display synchronous growth and are similar to *** shapes are mainly controlled by water depths and hydrodynamic *** greater the water depth,the more columnar the columns tend to *** strong hydrodynamic conditions decrease the growth rate of stromatolites,and they even stopped growth due to wave ***,pillared laminar textures(not Epiphyton),which consist of dendritic,micro-branched and microcolumnar stromatolites,are a common feature of the larger stratiform,stratiform-columnar and columnar *** alternations of laminae with different internal textures record subtle fluctuations in wat

关键词： Microbialites Manganese stromatolites Ediacaran Doushantuo Formation Yangtze Craton Growth mode

The Hubei Aging Study: a Cohort among Community-dwelling Seniors to Identify Risk Factors and Early Markers of Alzheimer’s Disease

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The Hubei Aging Study: a Cohort among Community-dwelling Sen...

硒蛋氨酸对胃癌细胞BGC-803化疗敏感性的影响

中国神经科学学会第十二届全国学术会议

作者： Dan Liu Yan Zeng Gui-rong Cheng Lang Xu ming Ou Yang qing-ming wu Brain and Cognitive Function Laboratory Wuhan University of Science and Technology Hubei Key Laboratory of Hazard Identification and Control for Occupational Disease

Objective Despite the high risk of dementia（mainly AD） in aged populations and the medical burden for care and treatment of this disease, the accumulated results still can’t establish a direct and complete correlation between related risk factors and physiopathological mechanisms of late-onset AD, it may be a key problem for the prevention and control of the AD. The project has been designed with a view to characterizing the risk factors and biochemical underpinnings of mild cognitive impairment（MCI） and dementia（mainly AD） in the first step of the survey, conducted among residents aged ≥ 65 in multiple communities of Hubei, China. To the end of the project, we will establish an AD risk early warning model that suitable for China, and explore the new blood polypeptide biomarkers for AD onset. Methods We are launching the Hubei Aging Project（HAP）, a cohort study of non-demented people aged 65–85. Given the exploratory nature of this project, multidimensional and machine learning techniques are applied, in addition to the traditional multivariate statistical methods. Standardised assessments of mental and physical health, and cognitive function are carried out including informant interviews. Besides health status, medical and family history, demographic and socio-cultural information are explored, as well as education, habitat network, and social behavior. Data relative to motor function, including balance, walk, limits of stability, history of falls and accidents are further detailed. Finally, biological examinations, including Apo E genetic polymorphism and amyloid peptide in the blood are carried out. In addition to standard blood parameters. Diagnoses of dementia and MCI are made using standard criteria via consensus diagnosis. Results A total of 3469 subjects were recruited between October 2014 and December 2016. Mean age was 74.4 years（SD 3.9）, and 63.5% of the subjects were women. Cognitive diagnoses at inclusion were as follows: normal cognition 93.0% and mild

关键词： Elderly cohort cognitive impairment risk factors of dementia Alzheimer’s disease big data technology

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世界华人消化杂志 2009年第34期17卷 3538-3542页

作者：陈晋吴清明武汉科技大学医学院湖北省武汉市430065 武汉科技大学附属天佑医院湖北省武汉市430064

目的:探讨硒蛋氨酸对胃癌细胞BGC-803化疗敏感性的影响.方法:用MTT法检测不同浓度的硒蛋氨酸对胃癌细胞BGC-803化疗敏感性的影响;免疫组织化学SP法检测化疗时补硒对胃癌细胞BGC-803中硒蛋白P表达的影响.结果:各浓度顺铂加硒蛋氨酸对胃... 详细信息

目的:探讨硒蛋氨酸对胃癌细胞BGC-803化疗敏感性的影响.方法:用MTT法检测不同浓度的硒蛋氨酸对胃癌细胞BGC-803化疗敏感性的影响;免疫组织化学SP法检测化疗时补硒对胃癌细胞BGC-803中硒蛋白P表达的影响.结果:各浓度顺铂加硒蛋氨酸对胃癌细胞BGC-803的增殖均有明显抑制作用,与单用同浓度顺铂组及空白对照组比较有差异性(均P<0.05),且呈一定的浓度、时间依赖性,当顺铂加硒蛋氨酸浓度为40mg/L+20mmol/L作用72h对胃癌细胞BGC-803的增殖抑制作用最强;同时,化疗时补充硒蛋氨酸能增强胃癌细胞BGC-803中硒蛋白P的表达.结论:硒蛋氨酸可增强顺铂对胃癌细胞BGC-803增殖的抑制作用,为其作为胃癌化疗的辅助剂提供了理论依据;硒蛋白P可能成为衡量胃癌化疗敏感性的指标之一.

关键词：硒蛋氨酸胃癌顺铂硒蛋白P

Plasmonic tandem heterojunctions enable high-efficiency charge transfer for broad spectrum photocatalytic hydrogen production

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Journal of Energy Chemistry 2025年第1期100卷 710-720页

作者： Wenxuan Chen Xiu-qing Qiao Guo Hui Bojing Sun Dongfang Hou Meidi Wang Xueqian wu Tao wu Dong-Sheng Li College of Materials and Chemical Engineering Key Laboratory of Inorganic Nonmetallic Crystalline and Energy Conversion Materials China Three Gorges University Hubei Three Gorges Laboratory College of Chemistry and Materials Science and Guangdong Provincial Key Laboratory of Supramolecular Coordination Chemistry Jinan University

Rational engineering of semiconductor photocatalysts for efficient hydrogen production is of great significance but still challenging,primarily due to the limitations in charge transfer ***,a fascinating plasmonic tandem heterojunction with the hc-CdS/Mo2C@C heterostructure is aimfully prepared for effectively promoting the charge separation kinetics of the CdS photocatalyst via the synergistic strategy of phase junction,Schottky junction,and photothermal *** difference in atomic configuration between cubic-CdS （c-CdS） and hexagonal-CdS （h-CdS） leads to effective charge separation through a typical Ⅱ charge transfer mechanism,and plasmonic Schottky junction further extracts the electrons in the hc-CdS phase junction to realize gradient charge ***,the photothermal effect of Mo2C@C helps to expand the light absorption,accelerate charge transfer kinetics,and reduce the hydrogen evolution energy *** carbon layer provides a fast channel for charge transfer and protects the photocatalyst from *** a result,the optimized hc-CMC photocatalyst exhibits a significantly high photocatalytic H2production activity of 28.63 mmol/g/h and apparent quantum efficiency of 61.8%,surpassing most of the reported *** study provides a feasible strategy to enhance the charge transfer kinetics and photocatalytic activity of CdS by constructing plasmonic tandem heterogeneous junctions.

关键词： Heterojunctions LSPR Photocatalyst Phase junction Schottky junction Photothermal effect

基于工程量清单计价模式下的已竣工工程数据库建设

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建筑技术 2017年第11期48卷 1227-1230页

作者：张晓东仲青吴明庆江苏开放大学建筑工程学院南京210024 江苏捷宏工程项目咨询有限公司南京210036

工程造价信息数据库是工程造价信息化的基础,应建有两大数据库,即收集信息的工程造价信息相关数据库以及应用于预测的计算方法数据库,其中收集、建立已完工程数据库是重点与难点。目前工程量清单计价模式已推行多年,其实质就是一个工程... 详细信息

工程造价信息数据库是工程造价信息化的基础,应建有两大数据库,即收集信息的工程造价信息相关数据库以及应用于预测的计算方法数据库,其中收集、建立已完工程数据库是重点与难点。目前工程量清单计价模式已推行多年,其实质就是一个工程造价的数据库,其业已形成的数据已成为已完工程数据库建立的重要来源,应将之纳入到工程造价信息数据库建设体系中。

关键词：工程量清单计价数据库项目编码备案制度 BIM