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Microbubble-enhanced ultrasound exposure improves gene transfer in vascular endothelial cells

World Journal of Gastroenterology 2006年第46期12卷 7508-7513页

作者： Fang Nie Hui-Xiong Xu qing Tang ming-De Lu Department of Medical Ultrasonics First Affiliated Hospital Sun Yat-Sen UniversityGuangzhou 510080 Guangdong Province China Department of Hepatobiliary Surgery First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080Guangdong Province China

AIM： To explore the effects of ultrasound exposure combined with microbubble contrast agent （SonoVue） on the permeability of the cellular membrane and on the expression of plasrnid DNA encoding enhanced green fluorescent protein （pEGFP） transfer into human umbilical vein endothelial cells （HUVECs）. METHODS： HUVECs with fluorescein isothiocyanatedextran （FD500） and HUVECs with pEGFP were exposed to continuous wave （1.9 MHz, 80.0 mW/cm^2） for 5 min, with or without a SonoVue. The percentage of FD500 taken by the HUVECs and the transient expression rate of pEGFP in the HUVECs were examined by fluorescence microscopy and flow cytornetry, respectively. RESULTS： The percentage of FDS00-positive HUVECs in the group of ultrasound exposure combined with SonoVue was significantly higher than that of the group of ultrasound exposure alone （24.0%± 5.5% vs 66.6% ± 4.1%, P 〈 0.001）. Compared with the group of ultrasound exposure alone, the transfection expression rate of pEGFP in HUVECs was markedly increased with the addition of SonoVue （16.1% ± 1.9% vs 1.5% ± 0.2%, P 〈 0.001）. No statistical significant difference was observed in the HUVECs survival rates between the ultrasound group with and without the addition of SonoVue （94.1% ± 2.3% vs 91.1% ± 4.1% ）. CONCLUSION： The cell membrane permeability of HUVECs and the transfection efficiency of pEGFP into HUVECs exposed to ultrasound are significantly increased after addition of an ultrasound contrast agent without obvious damage to the survival of HUVECs. This non- invasive gene transfer method may be a useful tool for clinical gene therapy of hepatic tumors.

关键词： Microbubble Ultrasound Gene transfer Human umbilical vein endothelial cell Enhanced green fluorescent protein

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QTL Mapping for Grain Size Traits Based on Extra-Large Grain Rice Line TD70

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Rice science 2013年第6期20卷 400-406页

作者： ZHANG Ya-dong ZHANG Ying-hui DONG Shao-ling CHEN Tao ZHAO qing-yong ZHU Zhen ZHOU Li-hui YAO Shu ZHAO Ling YU xing WANG Cai-lin Institute of Food Crops Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D CenterNanjing Branch of China National Center for Rice Improvement

Grain size traits, including grain length, grain width and grain thickness, are controlled by quantitative trait loci （QTLs）. Many QTLs relating to rice grain size traits had been reported, but their control mechanisms have not yet been elucidated. A recombinant inbred line （RIL） population of 240 lines, deriving from a cross between TD70, an extra-large grain size japonica line with 80 g of 1000-grain weight, and Kasalath, a small grain size indica variety, were constructed and used to map grain size QTLs to a linkage map by using 141 SSR markers in 2010 and 2011. Five QTLs for grain length, six for grain width and seven for grain thickness were detected distributing over chromosomes 2, 3, 5, 7, 9 and 12. Seven QTLs, namely qGL3.1, qGW2, qGW2.2, qGW5.1, qGW5.2, qGT2.3 and qGT3.1, were detected in either of the two years and explained for 56.19%, 4.42%, 29.41%, 10.37%, 7.61%, 21.19% and 17.06% of the observed phenotypic variances on average, respectively. The marker interval RM1347-RM5699 on chromosome 2 was found common for grain length, grain width and grain thickness; qGL3.1 and qGT3.1 were mapped to the same interval RM6080-RM6832 on chromosome 3. All 18 QTL alleles were derived from the large grain parent TD70. Most of the QTLs mapped in the present study were found the same as the genes previously cloned （GW2, GS3 or qGL3, GW5 and GS5）, and several were the same as the QTLs （GS7 and qGL-7） previously mapped. Three QTLs, qGL2.2 on chromosome 2, qGW9 and qGT9 on chromosome 9, were first detected. These results laid a foundation for further fine mapping or cloning of these QTLs.

关键词： rice extra-large grain recombinant inbred line grain trait quantitative trait locus

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Yu Gan Long Ameliorates Hepatic Fibrosis by Inhibiting PI3K/AKT,Ras/ERK and JAK1/STAT3 Signaling Pathways in CCl4-induced Liver Fibrosis Rats

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Current Medical Science 2020年第3期40卷 539-547页

作者： Hou-gang LI Peng-tao YOU Yu XI Yu CAI Yi-jun TU Meng-heng WANG Wan-ci SONG Tai-min QUAN Hui-ying REN Yan-wen LIU Han-xiong DAN Shi-qing XU Hubei Key Laboratory of Resources and Chemistry of Chinese Medicine Hubei University of Chinese MedicineWuhan430065China College of Pharmacy Hubei University of Chinese MedicineWuhan430065China Wuhan Junan Pharmaceutical Co. Ltd.Wuhan430023China

Yu Gan Long(YGL)is a Chinese traditional herbal formula which has been reported to attenuate liver fibrosis for many years and we have explored its anti-fibrotic mechanism through blocking transforming growth factor(TGF-β)in the previous *** the mechanisms associated with platelet-derived growth factor(PDGF)-BB remain *** this study,we further investigated the mechanism of YGL reducing carbon tetrachloride(CCl4)-induced liver fibrosis in *** results showed that YGL suppressed CCl4-induced upregulation of collagen IV(Col IV),type HI precollagen(PCHI),hyaluronuc acid(HA)and laminin(LN),which are implicated in liver ***,YGL reduced theα-smooth muscle actin(α-SMA)expression,which acts as the indicator of liver ***,YGL decreased the serum levels of hepatic stellate cell(HSC)mitogen PDGF-BB and inflammation cytokines,including TNF-α,IL-1β,*** involved in liver fibrosis,such as Ras,p-Raf-1,p-ERK1/2,p-JNK,p-P38,p-PI3K,p-AKT,p-JAKl,p-STAT3 were downregulated significantly after treatment with *** results indicated that YGL ameliorated CCl4-induced liver fibrosis by reducing inflammation cytokines production,and suppressing Ras/ERK,PI3K/AKT,and JAK1/STAT3 signaling pathways,which provided further evidence towards elucidation of the anti-fibrotic mechanism of YGL.

关键词： Yu Gan Long traditional Chinese herbal therapy liver fibrosis Ras/ERK PI3K/AKT JAK1/STAT3

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1960 MPa级Ф15.20 mm高强度低松弛预应力镀锌钢绞线研制

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金属制品 2024年第1期50卷 12-14页

作者：周鑫张青李权大吴光虎贵州钢绳股份有限公司贵州遵义563000

介绍1 960 MPa级Ф15.20 mm低松弛预应力镀锌钢绞线研发工艺。采用的Ф13.0 mm 82B专用盘条添加Cr和V元素,采用盐酸酸洗和锌系磷化表面处理工艺,中频加热温度380℃,张力120 kN,捻制速度40 m/min,生产的镀锌钢绞线力学性能和表面质量均符... 详细信息

介绍1 960 MPa级Ф15.20 mm低松弛预应力镀锌钢绞线研发工艺。采用的Ф13.0 mm 82B专用盘条添加Cr和V元素,采用盐酸酸洗和锌系磷化表面处理工艺,中频加热温度380℃,张力120 kN,捻制速度40 m/min,生产的镀锌钢绞线力学性能和表面质量均符合GB/T 33363—2016要求,满足客户对产品的技术使用要求。

关键词：钢绞线热镀锌表面处理抗拉强度

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Production of glycerol carbonate using crude glycerol from biodiesel production with DBU as a catalyst

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Chinese Journal of Chemical Engineering 2018年第9期26卷 1912-1919页

作者： Ying qing Houfang Lu Yingying Liu Changjun Liu Bin Liang Wei Jiang School of Chemical Engineering Sichuan University Institute of New Energy and Low Carbon Technology Sichuan University

The biodiesel production technology catalyzed by 1,8-diazabicycloundec-7-ene（DBU）is developed in this *** glycerol containing DBU and DBU/glycerol/CO2（DGC）ionic compounds reacts directly with dimethyl carbonate（DMC）to produce high value-added glycerol carbonate（GC）catalyzed by DBU and *** catalytic performance of DBU and DGC,as well as the kinetics of the reaction catalyzed by DBU,were *** results show that DGC has a weak catalytic effect on the transesterification of glycerol and *** the temperature is higher than 60℃,DGC catalyzes the reaction jointly with DBU,which is produced from the decomposition of *** has a good catalytic effect on the reaction between glycerol and DMC,with 90%conversion of glycerol and 84%selectivity to GC under the following conditions：DMC-to-glycerol molar ratio of 3：1,4.0%DBU（based on glycerol mass）,reaction time of 60 min,and reaction temperature of 40℃.The apparent kinetics results show that the activation energies are 30.95 kJ·mol^-1 and 55.16 kJ·mol^-1 for the forward and reverse GC generation reactions,respectively,and the activation energy of the decomposition reaction of GC to glycidol（GD）is 26.58 kJ·mol^-1.

关键词： Glycerol DBU Glycerol carbonate Apparent kinetics

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表达绿色荧光蛋白的坦布苏病毒的构建与鉴定

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动物医学进展 2024年第6期45卷 44-50页

作者：章丽娇田宇杰张兰香赵冬敏韩凯凯黄欣梅杨婧吴凤瑶刘青涛刘宇卓张小飞李银江苏省农业科学院兽医研究所江苏南京210014 兽用生物制品(泰州)国泰技术创新中心江苏泰州225300 贵州省农业科学院畜牧兽医研究所贵州贵阳550005 山东省日照市五莲县动物疫病预防控制中心山东五莲262300

报告病毒在高通量抗病毒药物筛选、活体动物体内成像及新型疫苗研发等方面具有重要应用价值。通过反向遗传操作技术,以坦布苏病毒JXSP株感染性克隆为基础,采用两种策略构建报告病毒,即分别在病毒基因组5′UTR和C蛋白基因、N S5基因和3′... 详细信息

报告病毒在高通量抗病毒药物筛选、活体动物体内成像及新型疫苗研发等方面具有重要应用价值。通过反向遗传操作技术,以坦布苏病毒JXSP株感染性克隆为基础,采用两种策略构建报告病毒,即分别在病毒基因组5′UTR和C蛋白基因、N S5基因和3′UTR间引入外源基因EGFP,通过融合PCR获得全长cDNA PCR产物,体外转录生成mRNA,转染至BHK-21拯救重组病毒并研究其生长特性。结果显示,利用5′UTR-C位点构建的报告病毒mRNA转染BHK-21细胞后能观察到明显的绿色荧光;报告病毒P0接种DEF细胞后可高强度表达绿色荧光蛋白,在BHK-21和DEF细胞上增殖速度略微低于亲本毒株,但差异不显著。结果表明,成功构建了1株坦布苏绿色荧光报告病毒rJXSP-5′EGFP,在BHK-21和DEF细胞上均能有效增殖。

关键词：坦布苏病毒绿色荧光蛋白报告病毒

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文冠果油神经酸乙酯对脱髓鞘小鼠脂肪酸、血脂的影响

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食品安全质量检测学报 2024年第2期15卷 235-242页

作者：陈方会杜俊民张芳荣贺文彬李钦青李春江胡美变山西中医药大学中药与食品工程学院晋中030619 山西中医药大学中医脑病山西省重点实验室晋中030619

目的探究文冠果油神经酸乙酯对双环己酮草酰二腙(cuprizone,CPZ)诱导的脱髓鞘小鼠脂肪酸、血脂的影响。方法SPF级雌性昆明小鼠50只,除空白组(KO)外,通过在饲料中加入0.25%CPZ饲喂雌性昆明小鼠造模并分为模型组(CPZ)、神经酸乙酯低剂量组... 详细信息

目的探究文冠果油神经酸乙酯对双环己酮草酰二腙(cuprizone,CPZ)诱导的脱髓鞘小鼠脂肪酸、血脂的影响。方法SPF级雌性昆明小鼠50只,除空白组(KO)外,通过在饲料中加入0.25%CPZ饲喂雌性昆明小鼠造模并分为模型组(CPZ)、神经酸乙酯低剂量组(NA-L,0.001912 mL/g)、神经酸乙酯中剂量组(NA-M,0.003824 mL/g)、神经酸乙酯高剂量组(NA-H,0.007648 mL/g),持续5周至实验结束。于造模2周后开始给药至第5周结束后取材,并通过气相色谱仪、血脂仪分别检测文冠果油神经酸乙酯对CPZ诱导的脱髓鞘小鼠脑组织、肝脏、心脏、血清、粪便里的脂肪酸含量及血液中血脂水平的影响。结果与KO组相比,CPZ组脱髓鞘小鼠脑组织、肝脏、心脏中神经酸、芥酸含量显著下降(P<0.05);与CPZ组相比,NA-L、NA-M、NA-H组小鼠脑组织、肝脏、心脏、血清、粪便中的神经酸含量均显著增加(P<0.05),其中KO、CPZ组小鼠血清、粪便中未检测到神经酸和芥酸,NA-L、NA-M、NA-H组小鼠血清、粪便中含有一定量的神经酸和芥酸;与KO组相比,CPZ组血脂升高;与CPZ组相比,NA-L、NA-M、NA-H组均可降低血液中的甘油三酯(triglyceride,TG)含量,升高高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)含量。结论在饲料中添加0.25%CPZ诱导小鼠脱髓鞘会导致小鼠血脂升高,降低小鼠体内神经酸、芥酸含量,脱髓鞘疾病与神经酸的积累有关,脱髓鞘疾病会导致神经酸的供应受损。在这种情况下,文冠果油神经酸乙酯提取物能够增加脱髓鞘小鼠体内神经酸及芥酸含量,提高脑组织不饱和脂肪酸含量,调节脱髓鞘小鼠血脂水平。此外,神经酸、芥酸参与脱髓鞘小鼠体内代谢,并在小鼠体内共存,文冠果油神经酸乙酯提取物可作为脱髓鞘小鼠补充神经酸的外源性物质。

关键词：文冠果油神经酸乙酯芥酸脂肪酸脱髓鞘

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基于UHPLC-Q-Exactive Orbitrap MS的复方一枝蒿颗粒化学成分快速鉴定

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中国现代中药 2024年第3期26卷 507-517页

作者：刘明娟李凯琳张敏唐苏女刘玲李晴郭俐兰杨建波李辉湖南医药学院药学院侗医药研究湖南省重点实验室湖南怀化418000 新疆维吾尔自治区药品检验研究院国家药品监督管理局中药(维药)质量控制重点实验室新疆乌鲁木齐830054 中国食品药品检定研究院北京100050

目的:采用超高效液相色谱-四极杆-静电轨道场离子阱质谱法对复方一枝蒿颗粒的化学成分进行分析。方法:采用Thermo Scientific Syncronis C_(18)色谱柱(100 mm×2.1 mm,1.7μm),流动相为0.1%甲酸-乙腈,梯度洗脱,流速为0.3 mL·min^(–1)... 详细信息

目的:采用超高效液相色谱-四极杆-静电轨道场离子阱质谱法对复方一枝蒿颗粒的化学成分进行分析。方法:采用Thermo Scientific Syncronis C_(18)色谱柱(100 mm×2.1 mm,1.7μm),流动相为0.1%甲酸-乙腈,梯度洗脱,流速为0.3 mL·min^(–1),柱温为45℃,进样量为2μL。在正负离子模式下采集复方一枝蒿颗粒的质谱数据,根据高分辨质谱提供的精确相对分子质量和碎片离子信息,结合对照品比对和相关文献报道对复方一枝蒿颗粒的化学成分进行分析。结果:共分析鉴定出90个化合物,包括2个氨基酸类、8个萜类、25个有机酸类、11个含氮类、44个黄酮类成分。其中,16个成分通过与对照品比对确认。结论:分析鉴定了复方一枝蒿颗粒的化学成分,为其质量控制及药效物质基础研究提供了依据。

关键词：复方一枝蒿颗粒超高效液相色谱-四极杆-静电轨道场离子阱质谱法化学成分

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基于Vague故障树的发动机系统可靠性分析

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汽车工程 2011年第3期33卷 267-270,275页

作者：张晓南刘安心李永青星常兴解放军理工大学工程兵工程学院南京210007

针对基于概率论的故障树分析(FTA)模型不适用于结构复杂、且失效行为具有不确定性汽车发动机系统的可靠性分析,将Vague集引入其故障树分析中,用Vague集描述底事件的失效行为,构造了基于三角形Vague集算术运算的最大和最小与门模糊算子,... 详细信息

针对基于概率论的故障树分析(FTA)模型不适用于结构复杂、且失效行为具有不确定性汽车发动机系统的可靠性分析,将Vague集引入其故障树分析中,用Vague集描述底事件的失效行为,构造了基于三角形Vague集算术运算的最大和最小与门模糊算子,并确定了Vague故障树分析的步骤、原则与方法。通过建立某典型发动机系统的Vague故障树分析(VFTA)模型,解决了故障树分析中部分事件故障判据及数据的模糊性问题。仿真结果表明VFTA模型比其它模糊FTA模型更灵活、有效。

关键词：发动机系统可靠性分析 Vague故障树

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Genetic Diversity of Wild Rice Species in Yunnan Province of China

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Rice science 2012年第1期19卷 21-28页

作者： CHENG Zai-quan YING Fu-you LI Ding-qing Yu Teng-qiong Fu Jian YAN Hui-jun ZHONG Qiao-fang ZHANG Dun-yu LI Wei-jiao HUANG xing-qi Biotechnology ＆ Genetic Resource Research Institute Yunnan Academy of Agricultural Sciences Kunming 650223 China Flower Research Institute Yunnan Academy of Agricultural Sciences Kunming 650223 China

Yunnan Province of China is one of the important centers for origin and evolution of cultivated rice worldwide. Wild rice is the ancestor of the cultivated rice. Many elite traits of wild rice have widened the genetic basis in cultivated rice. However, many populations of wild rice species have disappeared in the past few years. Therefore, the current status of wild rice resources should be updated and the genetic diversity of wild rice species should be examined for further germplasm preservation and utilization. Our investigations showed that the number of natural wild rice populations declined sharply in Yunnan Province during the past few years due to various reasons. Fortunately, one population of Oryza rufipogon, three of O officinalis and ten of O. granulata have been newly found in different ecological sites, which were confirmed by inter-simple sequence repeat （ISSR） marker analysis in this study. ISSR analysis and investigation of some important traits of nutritional values indicated that the genetic diversity of the currently existing wild rice resources in Yunnan is still rich. The demonstration of genetic diversity of wild rice by a combined use of geographical distribution, morphological traits, nutrition contents and ISSR markers would be helpful for the conservation and exploration of these important wild rice resources.

关键词： wild rice resource natural distribution genetic diversity genetic trait

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