检索结果-南通市图书馆

Testicular mixed germ cell tumor:A case report

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2023年第28期11卷 6902-6907页

作者： Qi-Fan Xiao Jie Li Bin Tang yu-qing zhu Department of International General Practice China-Japan Friendship HospitalBeijing 100029China Department of General Practice Xinggu Street Community Health Service CenterBeijing 101200China

BACKGROUND Testicular mixed germ cell tumors(TMGCTs)are rare malignant tumors that are more common in men aged 20–40 *** comprise two or more types of germ cell tumors that primarily affect the *** onset is undetectable;thus,early diagnosis is ***,early recognition and diagnosis substantially improve patient *** SUMMARY We evaluated a rare case of TMGCT in a male patient presenting with recurrent fever and left supraclavicular lymphadenectasis instead of testicular enlargement and pain,which may easily lead to *** report the clinical signs and symptoms,histopathological characteristics,and immunohistochemical results of this case of malignant *** Our case,which was typical with multiple components,along with a literature review,may serve as a basis for early diagnosis.

关键词： Germ cell tumor Testicular tumor Lymphadenectasis Immunotherapy Case report

Synthesis and Crystal Structure of a Zinc(II) Complex Salt with the Schiff Base of Picolinaldehyde N-oxide and Semicarbazone

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2005年第11期24卷 1271-1275页

作者： yu qing zhu Li-Gang BIAN He-Dong DENG Ji-Hua YANG Xiao-E GUO Gui-Quan LIANG Hong College of Chemistry and Chemical Engineering Guangxi Normal University Guilin Guangxi 541004 China

The title zinc（Ⅱ） complex salt [Zn（H2O）6]（ClO4）2-（PNOS）4, where PNOS is derived from picolinaldehyde N-oxide with semicarbazone, has been prepared and structurally characterized by X-ray single-crystal analysis. It crystallizes in triclinic, space group PI with a = 7.529（3）, b = 10.206（4）, c = 14.678（6）A, a = 86.293（6）, β= 87.686（7）, γ= 81.382（6）°, C28H44Cl2N16O22Zn, Mr = 1093.06, V = 1112.3（8） ,A^3 Z = 1, Dc = 1.632 g/cm^3, S = 1.089, μ（MoKa） = 0.773 mm^-1, F（000） = 564, the final R = 0.0438 and wR = 0.1076 for 3888 independent reflections with Rint = 0.0224. The crystal structure possesses a [Zn（H2O）6]^2＋ cation, two ClO4^- anions and four PNOSs. In the crystal structure, Zn^2＋ cation is located at the symcenter and coordinated by six water molecules. In [Zn（H2O）6]^2＋, an elongate octahedral complex cation, the average Zn-O bond length is 2.087（2） A. There exist a lot of H bonds in the structure, linking the cation [Zn（H2O）6]^2＋, anion ClO4^- and PNOS to form a 3D network.

关键词： zinc(Ⅱ) complex salt picolinaldehyde N-oxide semicarbazone crystal structure,Schiff base

Receptor-binding domain of SARS-Cov spike protein: Soluble expression in ***, purification and functional characterization

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第39期11卷 6159-6164页

作者： Jing Chen Lin Miao Jia-Ming Li Yan-Ying Li qing-yu zhu Chang-Lin Zhou Hong-qing Fang Hui-Peng Chen Institute of Biotechnology Academy of Military Medical Science Beijing 100071 China The Pharmaceutical University of China Nanjing 210009 Jiangsu Province China Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 China

AIM： To find a soluble and functional recombinant receptor-binding domain of severe acute respiratory syndrome-associated coronavirus （SARS-Cov）, and to analyze its receptor binding ability. METHODS： Three fusion tags （glutathione S-transferase, GST; thioredoxin, Trx; maltose-binding protein, MBP）, which preferably contributes to increasing solubility and to facilitating the proper folding of heteroprotein, were used to acquire the soluble and functional expression of RBD protein in Escherichia coli （BL21（DE3） and Rosetta-gamiB （DE3） strains）. The receptor binding ability of the purified soluble RBD protein was then detected by ELISA and flow cytometry assay. RESULTS： RBD of SARS-Cov spike protein was expressed as inclusion body when fused as TrxA tag form in both BL21 （DE3） and Rosetta-gamiB （DE3） under many different cultures and induction conditions. And there was no visible expression band on SDS-PAGE when RBD was expressed as MBP tagged form. Only GST tagged RBD was soluble expressed in BL21（DE3）, and the protein was purified by AKTA Prime Chromatography system. The ELISA data showed that *** antigen had positive reaction with anti-RBD mouse monoclonal antibody 1A5. Further flow cytometry assay demonstrated the high efficiency of RBD＇s binding ability to ACE2 （angiotensin-converting enzyme 2） positive Vero E6 cell. And ACE2 was proved as a cellular receptor that meditated an initial-affinity interaction with SARS-Cov spike protein. The geometrical mean of GST and *** binding to Vero E6 cells were 77.08 and 352.73 respectively. CONCLUSION： In this paper, we get sufficient soluble N terminal GST tagged RBD protein expressed in EcoliBL21 （DE3）; data from ELISA and flow cytometry assay demonstrate that the recombinant protein is functional and binding to ACE2 positive Vero E6 cell efficiently. And the recombinant RBD derived from *** can be used to developing subunit vaccine to block S protein binding with receptor and to neutralizing SARS-Cov infection.

关键词： Receptor-binding domain SARS-Cov Spike protein expression E.coli

Establishment of the Eukaryotic Cell Lines for Inducible Control of SARS-CoV Nucleocapsid Gene Expression

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2010年第5期25卷 361-368页

作者： Guo-hui CHANG Andrew Dividson Lei LIN Matt Wilson Stuart G Siddell qing-yu zhu State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China Department of Cellular and Molecular Medicine School of Medical and Veterinary Sciences University of Bristol Bristol BS8 1TD United Kingdom

In order to establish the eukaryotic cell lines for inducible control of SARS-CoV nucleocapsid gene *** recombinant plasmid of pTRE-Tight-SARS-N was constructed by using the plasmid p8S as the PCR template which contains a cDNA clone covering the nucleocapsid gene of SARS-CoV HKU-39449. Restriction enzymes digestion and sequence analysis indicated the recombinant plasmid of pTRE-Tight-SARS-N contained the nucleocapsid gene with the optimized nucleotide sequence which will improve the translation efficiency. Positive cell clones were selected by cotransfecting pTRE-Tight-SARS-N with the linear marker pPUR to BHK-21 Tet-on cells in the presence of puromycin. A set of double-stable eukaryotic cell lines (BHK-Tet-SARS-N) with inducible control of the SARS-CoV neucleocapsid gene expression was identified by using SDS-PAGE and Western-blot analysis. The expression of SARS-CoV nucleocapsid protein was tightly regulated by the varying concentration of doxcycline in the constructed double-stable cell line. The constructed BHK-Tet-SARS-N cell strains will facilitate the rescue of SARS-CoV in vitro and the further reverse genetic research of SARS-CoV.

关键词： SARS-CoV Nucleocapsid protein Inducible expression Double stable cell lines

在线全文

学校读者我要写书评

暂无评论

薄壁结构棱边强化效果

交通运输工程学报 2016年第5期16卷 57-65页

作者：郑玉卿朱西产胡强刘觐同济大学汽车学院上海201804 江西省科学院应用物理研究所江西南昌330029

基于方管理想化折叠单元模型、塑性挤压过程能量耗散分解法与棱边强化薄壁方管在轴向静态压溃作用下的能量平衡修正方程,分别导出了棱边强化薄壁方管的平均压溃力、吸能预测与棱边强化效果的理论表达式。建立了系列棱边强化薄壁方管准... 详细信息

基于方管理想化折叠单元模型、塑性挤压过程能量耗散分解法与棱边强化薄壁方管在轴向静态压溃作用下的能量平衡修正方程,分别导出了棱边强化薄壁方管的平均压溃力、吸能预测与棱边强化效果的理论表达式。建立了系列棱边强化薄壁方管准静态轴向压溃有限元模型,导入LS-DYNA程序进行弹塑性动力学仿真,获得了平均压溃力,分析了棱边应力强化的敏感性。将棱边强化技术应用于某车前保横梁和吸能盒相应棱边强度设计中,采用超高应力强化棱边和普通延性钢板组合去置换较高强度钢薄壁结构,在冲击试验验证过的有限元模型基础上,建立了棱边强化前保险杠子结构独立评价有限元模型,分别进行了棱边强化模型和原始模型的50km·h^(-1)正碰仿真试验。仿真结果表明:导出的平均压溃力公式可以预测棱边与平板屈服强度比为1~4的棱边强化薄壁方管轴向压溃力学特性,仿真结果与理论结果的最大偏差不超过5.66%;由前保险杠子结构正碰仿真结果得到了棱边强化前后近似的塑性变形模式与传力路径,能量吸收差值不超过0.3kJ。可见,选择性棱边应力强化后的普通延性钢前保险杠子结构有望实现等效置换较高强度钢前保险杠子结构。

关键词：汽车工程薄壁结构棱边强化数值仿真力学特性前保横梁吸能盒

Construction and Genetic Analysis of Murine Hepatitis Virus Strain A59 Nsp16 Temperature Sensitive Mutant and the Revertant Virus

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2011年第1期26卷 19-29页

作者： Guo-hui Chang Bao-jun Luo Pin Lu Lei Lin Xiao-yan Wu Jing Li Yi Hu qing-yu zhu State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China Changchun Changsheng Life Sciences Limited Changchun 130103 China

Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely studied model system for Coronavirus replication and pathogenesis. In this study,we created a MHV-A59 temperature sensitive (ts) mutant Wu"-ts18(cd) using the recombinant vaccinia reverse genetics system. Virus replication assay in 17C1-1 cells showed the plaque phenotype and replication characterization of constructed Wu"-ts18(cd) were indistinguishable from the reported ts mutant Wu"-ts18. Then we cultured the ts mutant Wu"-ts18(cd) at non-permissive temperature 39.5°C,which "forced" the ts recombinant virus to use second-site mutation to revert from a ts to a non-ts phenotype. Sequence analysis showed most of the revertants had the same single amino acid mutation at Nsp16 position 43. The single amino acid mutation at Nsp16 position 76 or position 130 could also revert the ts mutant Wu"-ts18 (cd) to non-ts phenotype,an additional independent mutation in Nsp13 position 115 played an important role on plaque size. The results provided us with genetic information on the functional determinants of Nsp16. This allowed us to build up a more reasonable model of CoVs replication-transcription complex.

关键词： Genetic analysis MHV-A59 Temperature-sensitive mutant Revertant Nonstructural proteins (Nsp)

Effect of pyrite content on chalcopyrite flotation under different regrinding conditions

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 49-57页

作者： Zejun Wang qing Shi Guofan Zhang yuxuan zhu Binbin Li School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources Central South UniversityChangsha 410083China

This study aimed to investigate the effect of varying pyrite(Py)content on copper(Cu)in the presence of different regrinding conditions,which were altered using different types of grinding media:iron,ceramic balls,and their mixture,followed by flotation in the cleaner *** flotation performance of rough Cu concentrate can be improved by changing the regrinding conditions based on the Py *** electron microscopy,X-ray spectrometry,ethylenediaminetetraacetic acid disodium salt extraction,and X-ray photoelectron spectroscopy studies illustrated that when the Py content was high,the use of iron media in regrinding promoted the generation of hydrophilic Fe OOH on the surface of Py and improved the Cu *** ceramic medium with a low Py content prevented excessive Fe OOH from covering the surface of chalcopyrite(Cpy).Electrochemical studies further showed that the galvanic corrosion current of Cpy-Py increased with the addition of Py and became stronger with the participation of iron media.

关键词： chalcopyrite regrinding media pyrite galvanic corrosion flotation

在线全文

学校读者我要写书评

暂无评论

某600MW机组冷端系统优化及应用研究

汽轮机技术 2017年第1期59卷 63-64,66页

作者：蒋文胡清宁玉琴朱文中杭州华电能源工程有限公司杭州310030 华电电力科学研究院杭州310030

介绍了一种冷端系统改造技术,并对该技术在国内某600MW机组投入运行后的经济性进行了分析,得出以下结论:冷端系统改造技术可大幅降低凝汽器运行压力,相比于改造前,凝汽器运行压力降低0.99kPa;该冷端系统改造技术投入运行后,机组年平均... 详细信息

介绍了一种冷端系统改造技术,并对该技术在国内某600MW机组投入运行后的经济性进行了分析,得出以下结论:冷端系统改造技术可大幅降低凝汽器运行压力,相比于改造前,凝汽器运行压力降低0.99kPa;该冷端系统改造技术投入运行后,机组年平均标煤耗降低2.48g/(kW·h),按机组年运行5500h、标煤单价600元/t计算,节能效益为515.6万元/年。

关键词：冷端系统凝汽器压力标煤耗节能效益

在线全文

学校读者我要写书评

暂无评论

高校青年教师思想政治工作的现状及对策

河北青年管理干部学院学报 2002年第2期14卷 52-53页

作者：于庆珠石家庄铁路工程职业技术学院工商管理系河北石家庄050041

高校青年教师在校园里有着教师和青年人的双重角色特征。他们既是教育的主体,承担着教书育人的职责,同时因为他们是年轻人,也面临着知识的升级与更新,世界观的重塑与定型。长期以来,人们只重视了他们的青年人特征,注重了他们业务上的发... 详细信息

高校青年教师在校园里有着教师和青年人的双重角色特征。他们既是教育的主体,承担着教书育人的职责,同时因为他们是年轻人,也面临着知识的升级与更新,世界观的重塑与定型。长期以来,人们只重视了他们的青年人特征,注重了他们业务上的发展和提高,而忽视了他们也是受教育者的特征,忽视了他们思想政治素质的提高。目前高校青年教师思想政治工作的现状,告诉我们必须要充分认识高校青年教师思想政治工作的重要性和紧迫性,把青年教师的思想和业务工作做好、做精,为高校的持续发展奠定良好的基础。

关键词：高校青年教师思想政治工作角色特征教书育人受教育者思想政治素质校园业务现状持续发展