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In situ observation of surface structures of cardiovascular endothelial cells with atomic force microscope

Journal of Geriatric Cardiology 2009年第3期6卷 178-181页

作者： tong Yin Jin Luo Ya-Min Ma xiao-Long Ji Yu-Sheng Zhao Shi-Wen Wang Institute of Geriatric Cardiology Department of Cardiovascular Surgery General Hospital of PLA Beijing 100853 China Institute of Nanomedicine General Hospital o fArmed Police Forces Beo＇ing 100039 China

Objective To observe the surface structures of cardiovascular endothelial cells in situ with atomic force microscope （AFM）. Methods Fresh aorta and aortic valve were dissected from 10 healthy male New Zealand white rabbits. Before fixed in 1% formaldehyde, the fresh tissues were washed in the buffer phosphate solution. Under general microscope, the fixed aorta or valve was spread on the double side stick tape which had already been stuck on the glass slide. The intima of aorta or the aorta side of valve was towards upside, Then the specimen was dried under 37 degrees centigrade in an attemperator and was washed with pure water. After dried again, the specimen was loaded on the platform ofNanoScope Ⅲa AFM and was scanned in tapping mode with the scanning speed of 0.5 HZ. Results The surface structures of endothelial cell on the fixed and dried tissue could be observed clearly in situ with AFM. aortic endothelial cells were large, branched and arranged sparsely and parallel to the direction of blood flow, whereas endothelial cells on aorta valve surface were small, less branched and arranged intensively and vertical to the direction of blood flow. When the scanning range was dwindled, granular ultra-structures could be observed on the surface of endothelial cells, and, as the scanning range was dwindled further, fissure and convolution could be seen on the surface of granules from aortic endothelial cells. Centre cavity and surrounding swelling volcano-like structure could be seen on the surface of granules from endothelial cells of aortic valve. Conclusions It＇s feasible to observe the surface ultra-structures of cardiovascular endothelial cells in situ with AFM and morphological information provided by AFM might be of clinical value in future histopathological diagnosis（JGeriatr Cardiol2009; 6：178-181）.

关键词： atomic force microscope endothelial cell

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Upregulation of UBAP2L in Bone Marrow Mesenchymal Stem Cells Promotes Functional Recovery in Rats with Spinal Cord Injury

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Current Medical Science 2018年第6期38卷 1081-1089页

作者： Guan-lin LIN Huan WANG Jun DAI xiao LI Ming GUAN qing DING Huai-xi WANG Huang FANG Department of Orthopaedics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Post-translational modifications of cellular proteins with ubiquitin or ubiquitin-like proteins regulate many cellular processes,such as cell proliferation,differentiation,apoptosis, signal transduction,intercellular immune recognition,inflammatory response,stress response,and DNA ***4/UBAP2L is an important member in the family of ubiquitin-like proteins,and its biological function remains *** study aimed to investigate the effect of UBAP2L on spinal cord injury (SCI).At first,rat bone marrow mesenchymal stem cells (BMSCs)were infected with adeno-associated virus to induce over-expression of ***,the infected BMSCs were transplanted into rats suffering from semi-sectioned *** results showed that the over-expression of Nice4 significantly promoted the proliferation and differentiation of BMSCs. In addition,the transplantation of infected BMSCs into the injured area of SCI rats improved the function repair of ***,the immunohistochemical and hematoxylin-eosin staining and RT-PCR results showed that the number of neuronal cells,oligodendrocytes,and astrocytes was significantly increased in the injured area,along with significantly upregulated expression ofcyclin D1 and p38 mitogen-activated protein kinase (MAPK).Meanwhile,the expression of caspase 3 protein was significantly *** conclusion,the over-expression of Nice4 gene can promote the functional recovery in SCI rats by promoting cell proliferation and inhibiting apoptosis. The results of this study indicate an alternative option for the clinical treatment of SCI.

关键词： bone marrow mesenchymal stem cells spinal cord injury ubiquitin-associated protein 2-like apoptosis proliferation

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The current status of foot self-care knowledge, behaviours, and analysis of influencing factors in patients with type 2 diabetes mellitus in China

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International Journal of Nursing Sciences 2014年第3期1卷 266-271页

作者： Rao Li Li Yuan xiao-Hui Guo qing-qing Lou Fang Zhao Li Shen Ming-Xia Zhang Zi-Lin Sun Department of Endocrinology West China HospitalSichuan UniversityChengduChina Department of Endocrinology the First Hospital Affiliated to Peking UniversityBeijingChina Department of Health Education Combine Traditional Chinese and Western Medicine HospitalJiangsuChina Department of Endocrinology China-Japan Friendship HospitalBeijingChina Department of Nursing Tsinghua Chang Gung HospitalBeijingChina Department of Endocrinology Peking University People's HospitalBeijingChina Department of Endocrinology Southeast University Affiliated Zhongda HospitalJiangsuChina

Purpose:To investigate the knowledge and behaviours on foot self-care in patients with type 2 diabetes mellitus;analyse the influential factors,and provide information for the intervention ***:A total of 5961 patients with type 2 diabetes mellitus from 144 hospitals in China were included in the *** investigation content included patients'demographic data,foot self-care knowledge and *** investigation tools were the questionnaires on the general diabetes information,on the foot self-care knowledge,and on the foot selfcare ***:The foot self-care knowledge was medium and the foot self-care behaviour was *** status of knowledge and behaviours were influenced by education,duration of diabetes mellitus,periodic inspection,and education about diabetic *** analysis demonstrated that there was positive correlation between knowledge score and behaviour score(r?0.27,p<0.001).Conclusions:The status of foot self-care knowledge and behaviours are not *** to the patients'own characteristics,the theory of knowledge,attitude and practice applies to encouraging patients to go for periodic inspection and education about diabetic complications so as to enhance the knowledge and promote the self-care behaviours.

关键词： Behaviour Diabetic foot Knowledge Self-care

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Predictive value of presepsin and acylcarnitines for severity and biliary drainage in acute cholangitis

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World Journal of Gastroenterology 2023年第16期29卷 2502-2514页

作者： Han-Yu Zhang Hong-Li xiao Guo-Xing Wang Zhao-qing Lu Miao-Rong Xie Chun-Sheng Li Department of Emergency Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China

BACKGROUND Bacteremia,which is a major cause of mortality in patients with acute cholangitis,induces hyperactive immune response and mitochondrial *** is responsible for pathogen recognition by innate *** are established mitochondrial *** To clarify the early predictive value of presepsin and acylcarnitines as biomarkers of severity of acute cholangitis and the need for biliary *** Of 280 patients with acute cholangitis were included and the severity was stratified according to the Tokyo Guidelines *** presepsin and plasma acylcarnitines were tested at enrollment by chemiluminescent enzyme immunoassay and ultra-high-performance liquid chromatography-mass spectrometry,*** The concentrations of presepsin,procalcitonin,short-and medium-chain acylcarnitines increased,while long-chain acylcarnitines decreased with the severity of acute *** areas under the receiver operating characteristic curves(AUC)of presepsin for diagnosing moderate/severe and severe cholangitis(0.823 and 0.801,respectively)were greater than those of conventional *** combination of presepsin,direct bilirubin,alanine aminotransferase,temperature,and butyryl-L-carnitine showed good predictive ability for biliary drainage(AUC:0.723).Presepsin,procalcitonin,acetyl-L-carnitine,hydroxydodecenoyl-Lcarnitine,and temperature were independent predictors of bloodstream *** adjusting for severity classification,acetyl-L-carnitine was the only acylcarnitine independently associated with 28-d mortality(hazard ratio 14.396;P<0.001)(AUC:0.880).Presepsin concentration showed positive correlation with direct bilirubin or *** Presepsin could serve as a specific biomarker to predict the severity of acute cholangitis and need for biliary ***-L-carnitine is a potential prognostic factor for patients with acute *** immune response was associated with mitoc

关键词： Acute cholangitis Severity Biliary drainage Presepsin Acylcarnitines

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Dysregulated RUNX1 Predicts Poor Prognosis by Mediating Epithelialmesenchymal Transition in Cervical Cancer

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Current Medical Science 2022年第6期42卷 1285-1296页

作者： Ling-ling ZHENG Lei CAI xiao-qing ZHANG Zhe LEI Chang-sheng YI Xing-dang LIU Ji-gang YANG Department of Nuclear Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing100050China Department of Nuclear Medicine Huashan HospitalFudan UniversityShanghai200040China Reproductive Medicine Center Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Thoracic Surgery the Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhou450003China

Objective Runt-related transcription factor 1(RUNX1)has been proven to be over-expressed and vital in many ***,its role in cervical cancer is still *** Some online databases(Oncomine,GEPIA,UALCAN,LinkedOmics,and others)were used to explore the expression level,prognostic significance,and gene mutation characteristics of RUNX1 in cervical *** protein levels of RUNX1 in cervical cancer were measured by immunohistochemistry(IHC).The functional changes of cervical cancer cells were measured in vitro after decreasing *** Bioinformatic results revealed that RUNX1 was upregulated in cervical cancer compared to normal ***,over-expression of RUNX1 was significantly correlated with cervical cancer patients’clinical parameters(e.g.,individual cancer stages,patients’age,nodal metastasis status,and others).Meanwhile,functional enrichment analysis of RUNX1-related genes indicated that RUNX1 was mainly involved in the epithelial-mesenchymal transition(EMT)process in cervical ***,RUNX1 may be upregulated by hsamiR-616-5p and hsa-miR-766 identified by miRDB,TargetScan,and ***,RUNX1 was upregulated in cervical cancer compared to normal tissues by IHC in collected cervical cancer *** invasion and migration abilities of cervical cancer cells were significantly reduced by repressing EMT after knocking down RUNX1 in *** RUNX1 was highly expressed in cervical cancer,and upregulated RUNX1 could significantly promote the invasive abilities of cervical cancer cells by inducing ***,RUNX1 may be a potential biomarker for early diagnosis and targeted therapy of cervical cancer.

关键词： runt-related transcription factor 1 cervical cancer epithelial-mesenchymal transition bioinformatics

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上呼吸道益生菌Biohalo23用于呼吸道感染及中耳炎的专家共识——现有循证科学依据及未来研究建议

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中国合理用药探索 2024年第8期21卷 33-47页

作者：王强张亚同成晓玲郭智刘洋夏立红刘志刚郑俊青张子和孙凯沈关心武汉科技大学医学院武汉430065 北京医院北京100730 首都医科大学附属北京儿童医院北京100045 华中科技大学协和深圳医院深圳518052 武汉亚心总医院武汉430056 山东中医药大学附属医院济南250014 济南市妇幼保健院济南250001 山东省妇幼保健院济南250000 山东第一医科大学第一附属医院济南250014 华中科技大学同济医学院武汉430030

呼吸道菌群决定着宿主是否成为呼吸道感染易感体质,并与宿主免疫系统共筑成人体对抗感染的第一道防线。呼吸道菌群特征的塑造与生命早期的无症状病毒暴露、生命早期呼吸道感染、过敏体质以及孕期或生命早期的抗生素暴露息息相关,这些因... 详细信息

呼吸道菌群决定着宿主是否成为呼吸道感染易感体质,并与宿主免疫系统共筑成人体对抗感染的第一道防线。呼吸道菌群特征的塑造与生命早期的无症状病毒暴露、生命早期呼吸道感染、过敏体质以及孕期或生命早期的抗生素暴露息息相关,这些因素也是日后形成反复呼吸道感染或过敏体质的重要因子。近年来,有许多研究试图探索人体微生态干预对调节宿主免疫及预防呼吸道感染的效果,但由于研究之间存在非同质化且不一致的再现性,使医生对于推荐益生菌给患者持有不同的见解。益生菌成品研究作为近几年学术界推荐的科研循证方法,是推动益生菌产业良性发展的有效方法,研究结论更符合真实世界使用结果,利于医生临床使用和消费者选择参考。鉴于中国和欧洲真实世界中的上呼吸道益生菌Biohalo23临床实践经验与患者的积极正向反馈,本专家组认为,具有循证科学依据并在多项研究中取得一致及积极临床研究结果的上呼吸道益生菌Biohalo23,可被视为医生在标准药物治疗基础上推荐给需要的患者以提高呼吸道感染相关生活质量并减轻呼吸道感染易感负担的“工具”,同时也鼓励医生关注呼吸道微生态在医学应用发展的新研究领域,以及呼吸道菌群干预可提供更佳疾病管理的巨大潜力。

关键词：上呼吸道益生菌Biohalo23 呼吸道感染中耳炎专家共识循证科学依据

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Clustering of Inflammatory Biomarkers and Risk of Hypertension in a Mongolian Population in China

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Biomedical and Environmental Sciences 2012年第5期25卷 562-568页

作者： ZHU xiao Hong JIAO Yang LI Hong Mei WANG Ai Li WANG Gui Yan tong Wei Jun ZHANG Yong Hong Department of Epidemiology School of Public Health Medical College of Soochow University Suzhou 215123 Jiangsu China Suzhou Industrial Park Centers for Disease Control and Prevention (Institute of Health Inspection and Supervision) Suzhou 215021 Jiangsu China Department of Health Care Xiamen Maternal and ChiM Care Hospital Xiamen 361000 Fujian China

Abstract Objective There is little knowledge on whether there is clustering of inflammatory biomarkers, such as C-reactive protein （CRP）, soluble intracellular adhesion molecule1-1 （slCAM-1）, and angiotensin It （Ang Ⅱ）, in individuals with hypertension in the Mongolian population. In the present study, we investigated this relationship in a Mongolian population in China. Methods A total of 2589 adult Mongolians, aged 20 years and older, were recruited as study participants. Data on demographics, lifestyle, family history of hypertension, blood pressure, and blood chemistry were collected, and inflammatory biomarkers were measured in all participants. Results The proportion of subjects with increased levels of two or three biomarkers was significantly higher in those with hypertension （21.0% and 6.0%, respectively） than in those with prehypertension （12.7% and 0.5%, respectively） or normotension （8.1% and 0.2%, respectively）. The multivariate adjusted odds ratios （95% confidence interval） of hypertension associated with increased levels of one, two or three biomarkers were 0.94 （0.72-1.22）, 1.42 （0.93-2.16）, and 11.08 （1.45-84.80）, respectively, compared with subjects with no increase in any biomarker. Conclusion Hypertension was associated with a cluster of inflammatory biomarkers in the Mongolian population.

关键词： C-reactive protein Soluble intracellular adhesion molecule-i Angiotensin II Hypertension Mongolia

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生物样品中的百草枯和敌草快的反相高效液相色谱-二极管阵列同时测定法

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环境与健康杂志 2024年第3期41卷 259-262页

作者：张玉丹耿琪王超洋杨柳肖庆锋河南省第三人民医院(河南省职业病医院) 河南省化学毒物检测医学重点实验室郑州450052

目的建立和优化了pH中性条件下同时测定生物样品中百草枯和敌草快的反相高效液相色谱-二极管阵列检测法。方法采用35%的高氯酸对尿样、胃洗液、血液样品进行酸脱蛋白后,以乙腈-磷酸缓冲液(体积比为4∶96,含5 mmol/L庚烷磺酸钠,pH 6)为... 详细信息

目的建立和优化了pH中性条件下同时测定生物样品中百草枯和敌草快的反相高效液相色谱-二极管阵列检测法。方法采用35%的高氯酸对尿样、胃洗液、血液样品进行酸脱蛋白后,以乙腈-磷酸缓冲液(体积比为4∶96,含5 mmol/L庚烷磺酸钠,pH 6)为流动相进行色谱分离,柱温30℃,流速为1.2 ml/min,百草枯在257 nm,敌草快在309 nm下同时检测。结果在0.05~5.0μg/ml的范围内,所得百草枯的回归方程为y=32842x-2603.2,r=0.9994;所得敌草快的回归方程为y=26729x-999.52,r=0.9997。该方法对百草枯、敌草快的检出限分别为0.015μg/ml、0.017μg/ml;平均回收率分别为91.1%~98.7%和88.9%~104.6%,批内RSD分别为0.79%~7.61%和1.98%~5.41%,批间RSD分别为1.04%~7.08%和2.19%~7.40%。结论该方法操作简便、准确度较好,精密度和灵敏度均较高,适用于临床上百草枯和敌草快样品的快速检测与浓度监测。

关键词：百草枯敌草快尿血胃洗液色谱法,高效液相二极管阵列

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Singularity of Some Software Reliability Models and Parameter Estimation Method

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Wuhan University Journal of Natural Sciences 2000年第1期5卷 35-40页

作者： XU Ren-zuo ZHOU Rui YANG xiao-qing (State Key Laboratory of Software Engineering, Wuhan University, Wuhan 430072, China) Wuhan Univ Wuhan China

According to the principle, “The failure data is the basis of software reliability analysis”, we built a software reliability expert system (SRES) by adopting the artificial intelligence technology. By reasoning out the conclusion from the fitting results of failure data of a software project, the SRES can recommend users “the most suitable model” as a software reliability measurement model. We believe that the SRES can overcome the inconsistency in applications of software reliability models well. We report investigation results of singularity and parameter estimation methods of experimental models in SRES.

关键词： software reliability measurement models software reliability expert system singularity parameter estimation method path following method maximum likelihood ML-fitting algorithm

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复材风扇叶片榫头元件低周疲劳和损伤模式

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南京航空航天大学学报 2024年第3期56卷 486-493页

作者：肖贾光毅唐旭张煜坤陈勇中国航发商用发动机有限责任公司上海200241 上海交通大学机械与动力工程学院上海201100 燃气轮机与民用航空发动机教育部工程研究中心上海201100

为考核层合结构复材风扇叶片在1.5倍离心载荷下的低周疲劳寿命,制备一批风扇叶片,在薄弱部位取样。通过建立层合结构风扇叶片有限元模型,模拟榫头的应力状态,从而确定拉伸载荷水平,开展拉-拉载荷低周疲劳测试。在相邻铺层间引入内聚力单... 详细信息

为考核层合结构复材风扇叶片在1.5倍离心载荷下的低周疲劳寿命,制备一批风扇叶片,在薄弱部位取样。通过建立层合结构风扇叶片有限元模型,模拟榫头的应力状态,从而确定拉伸载荷水平,开展拉-拉载荷低周疲劳测试。在相邻铺层间引入内聚力单元,并考虑榫头试样单向带面内损伤,模拟损伤变量随循环数累积的过程,增加虚拟拉伸载荷,得到榫头低周疲劳失效时刻的损伤状态。试验结果说明榫头元件形成分层裂纹的平均寿命为17207次循环,首次出现分层裂纹的位置基本一致。数值仿真结果表明榫头单向带基体和层间损伤累积引起裂纹扩展,导致低周疲劳失效。本研究可为建立层合结构复材风扇叶片低周疲劳寿命试验流程和疲劳失效判据提供支撑。

关键词：低周疲劳寿命风扇叶片复合材料有限元法渐进损伤分析

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