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Propofol blocks apoptosis and Bcl-2 and Bax expression induced by hypoxia-reoxygenation in primary cultures of rat hippocampal astrocytes

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Neural Regeneration Research 2009年第7期4卷 518-522页

作者： qing li Juying liu Long Zhou Chengming Qin Department of Anesthesiology Taihe Hospital of Yunyang Medical Coflege Shiyan 442000 Hubei Province China

BACKGROUND： Cerebral hippocampal astrocytes are more *** ischemic injury than neurons. Hypoxic-ischemic brain injury induces profound astrocyte apoptosis, and propofol may protect against astrocyte apoptosis. OBJECTIVE： To verify the protective effects of propofol against astrocyte apoptosis and to investigate anti-apoptotic Bcl-2 and pro-apoptotic Bax expression in primary cultures of rat hippocampal astrocytes exposed to hypoxia-reoxygenation for different periods of time following propofol treatment. DESIGN, TIME, AND SETTING： In vitro neural immunocytochemistry was performed at the Central Laboratory of Yunyang Medical College between September 2007 and March 2008.MATERIALS： A total of 30 Wistar rats, aged 1-3 days, wJth equal numbers of males and females, were included for isolation and culture of .hippocampal astrocytes. METHODS： Hippocampal astrocytes were purified and cultured for 3 weeks and treated with four culture conditions： 50 μL Hank＇s solution （normal control）; 0.2 mL/L Intralipid; 50 μL Hank＇s solution for 10 minutes followed by hypoxic incubation for 4 hours and normoxic incubation for 12, 24, 36, 48, 60 or 72 hours; propofol （250 μmol/L final） for 10 minutes followed by hypoxic incubation for 4 hours and normoxic incubation for 12, 24, 36, 48, 60 and 72 hours. MAIN OUTCOME MEASURES：（1） Morphologic changes in hippocampal astrocytes. （2） Levels of astrocyte apoptosis and Bcl-2 and Bax expression. RESULTS： Hypoxia and reoxygenation increased apoptosis over time, with Bcl-2 expression peaking at 24 hours and decreasing gradually （P 〈 0.01 ）; Bax expression peaked at 72 hours （P 〈 0.01）; the ratio of Bcl-2/Bax was 1.4, 0.8, and 0.6, respectively, at 24, 48 and 72 hours. Non-apoptotic astrocytes showed significant proliferation and swelling. Propofol treatment decreased apoptosis after hypoxia-reoxygenation （P 〈 0.01）, as well as Bct-2 and Bax expression （P 〈 0.05, P 〈 0.01）, with Bcl-2/Bax ratios of 1.6-1.8. Propofol treatmentalso

关键词： propofol hippocampal astrocyte apoptosis hypoxia and re-oxygenation Bcl-2 Bax

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Diesel Pollution Biodegradation: Synergetic Effect of Mycobacterium and Filamentous Fungi

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Biomedical and Environmental Sciences 2008年第3期21卷 181-187页

作者： You-qing li HONG-FANG liu ZHEN-LE TIAN li-HUA ZHU YING-HUI WU HE-qing TANG Department of Chemistry Huazhong University of Science and Technology Wuhan 430074 Hubei China

Objective To biodegrade the diesel pollution in aqueous solution inoculated with Mycobacterium and filamentous fungi. Methods Bacteria sampled from petroleum hydrocarbons contaminated sites in Karamay Oilfield were isolated and identified as Mycobacterium hyalinum （MH） and cladosporium. Spectrophotometry and gas chromatography （GC） were used to analyze of the residual concentrations of diesel oil and its biodegradation products. Results From the GC data, the values of apparent biodegradation ratio of the bacterial strain MH to diesel oil were close to those obtained in the control experiments. Moreover, the number of MH did not increase with degradation time. However, by using n-octadecane instead of diesel oil, the real biotic degradation ratio increased to 20.9% over 5 days of degradation. Cladosporium strongly biodegraded diesel oil with a real degradation ratio of up to 34% after 5 days treatment. When the two strains were used simultaneously, a significant synergistic effect between them resulted in almost complete degradation of diesel oil, achieving a total diesel removal of 99% over 5 days of treatment, in which one part of about 80% and another part of about 19% were attributed to biotic and abiotic processes, respectively. Conclusion The observed synergistic effect was closely related to the aromatics-degrading ability of Cladosporium, which favored the growth of MH and promoted the bioavailability of diesel oil

关键词： Biodegradation Diesel Synergistic effect Mycobacterium hyalinum Fungi

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Effect of Seawater Stress on Physiological and Biochemical Responses of Five Jerusalem Artichoke Ecotypes

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Pedosphere 2009年第2期19卷 208-216页

作者： LONG Xiao-Hua CHI Jin-He liu ling li qing liu Zhao-Pu College of Resources and Environmental Sciences Nanjing Agricultural University Nanjing 210095 China

Three treatments consisting of 0%,15%,and 30%seawater were investigated to analyse the ecotypic variabilities among five populations of Jerusalem artichoke(Helianthus tuberosus)regarding their responses to seawater stress under a hydroponic culture *** were done 2,4,and 6 days after *** 15%and 30%seawater treatments reduced the growth rates of roots and shoots of *** *** activities of superoxide dismutase, peroxidase,and catalase majored in the leaves were stimulated under the seawater *** electrolyte leakage and malondialdehyde contents of the leaves were also stimulated owing to seawater *** contents of proline and soluble- sugars in the leaves increased significantly with increasing seawater *** concentrations of Na+,K+,and Cl-in the aerial parts and roots increased with an increase in the seawater concentration throughout the experimental *** were ecotypic differences among the five populations of *** as evidenced by the analyses of the above items in both aerial parts and roots under seawater *** magnitude of the ecotypic variance components indicated that a substantial proportion of the total variation for these physiological and biochemical responses were owing to ecotype,indicating the possibility of improvement through hybridization and selection.

关键词： antioxidative enzyme Helianthus tuberosus inorganic and organic solutes seawater stress

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Effect of propofol on glutamate-induced activation and related inflammatory cytokines of astrocytes from spinal cord dorsal horn

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Neural Regeneration Research 2008年第9期3卷 954-957页

作者： Chengming Qin qing li Juying liu Tao Zhu Yong Xiang Department of Anesthesiology Taihe Hospital of Yunyang Medical College Shiyan 442000 Hubei Province China

BACKGROUND： Astrocytes participate in central nervous system-mediated physiological or pathological processes, such as pain. Activated dorsal horn astrocytes from the spinal cord produce nerve active substances and proinflammatory cytokines, such as interleukin-lbeta （IL-1 β ）, IL-6, and tumor necrosis factor- α （TNF-α ）, which play important roles in pain transduction and regulation. OBJECTIVE： To investigate the effects of different doses of propofol on activation of cultured spinal cord dorsal horn astrocytes induced by glutamate, as well as changes in IL-1β, IL-6, and TNF- α, and 1L-10 （anti-inflammatory cytokine） expression in rats, and to explore the dose relationship of propofol. DESIGN, TIME AND SETTING： The cellular and molecular biology experiment was performed at the Central Laboratory of Yunyang Medical College between March 2006 and December 2007. MATERIALS： Forty healthy, Wistar rats, aged 2-3 days, were selected. Propofol was provided by Zeneca, UK; glutamate by Sigma, USA; EPICS XL flow cytometry by Beckman culture, USA; rabbit-anti-mouse glial fibrillary acidic protein （GFAP） antibody kit and inflammatory cytokine detection kit were provided by Zhongshan Biotechnology Company Ltd., Beijing; multimedia color pathologic image analysis system was a product of Nikon, Japan. METHODS： Astrocytes were harvested from T11- L6 spinal cord dorsal horn of Wistar rats and incubated for 3 weeks. The cells were divided into seven groups, according to various treatment conditions： control group was cells cultured in Hank＇s buffered saline solution; intralipid group was cells cultured in intralipid （0.2 mL/L）; glutamate group was cells cultured with 100 u mol/L glutamate; propofol group was cells cultured with 250 u mol/L propofol; three glutamate plus propofol groups were cultured in 100 11 mol/L of glutamate, followed by 5, 25, and 250 u mol/L of propofol 10 minutes later. MAIN OUTCOME MEASURES： GFAP-labeled astrocytes were analyzed using a multimedia pathology im

关键词： astrocyte glutamate inflammatory cytokine propofol spinal cord dorsal horn

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Metabolic Regulation of Mammary Gland Epithelial Cells of Dairy Cow by Galactopoietic Compound Isolated from Vaccariae segetalis

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Agricultural Sciences in China 2011年第7期10卷 1106-1116页

作者： TONG Hui-li GAO Xue-jun li qing-zhang liu Jie li Nan WAN Zhong-ying Key Laboratory of Dairy Science Ministry of Education/Life College Northeast Agricultural University Harbin 150030 P.R.China

In previous experiment, we isolated a compound dibutyl phthalate （DBP） from Vaccaria segetalis which had galactopoietic function on mammary gland epithelial cells of dairy cow （DCMECs）. In this experiment, we ascertained the metabolic regulation function of DBP on DCMECs. Many genes related to lactation including Stat5, AMPK, b-casein, Glut1, SREBP-1, PEPCK, and ACC were detected by real-time PCR. Furthermore, Stat5 and AMPK were detected by Western blot and immunofluorescence co-localization, respectively. The results showed that DBP stimulates the expression of Stat5 and p-Stat5, thus activates Stat5 cell signal transduction pathway and stimulates b-casein synthesis. DBP also raises the activities of Glut1 and AMPK to stimulate glucose uptake and glycometabolism and activates the expression of AMPK downstream target genes PEPCK and ACC and expression of SREBP-1 to stimulate milk fat synthesis. In addition, the activities of HK, G-6-PDH, ICDH, ATPase, and energy charges were stimulated by DBP to increase the energy metabolism level of DCMECs. The results showed DBP stimulates energy metabolism related to galactopoietic function in DCMECs.

关键词： Vaccaria segetalis DBP dairy cow mammary gland epithelial cell metabolic regulation

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Angiofibrolipoma of the spermatic cord

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Asian Journal of Andrology 2009年第6期11卷 746-747页

作者： qing-li liu Bao Tian Hui Zhang De-Shan Qiao Department of Urology The First Hospital of Qinhuangdao Qinhuangdao 066000 China Department of Pathology The First Hospital of Qinhuangdao Qinhuangdao 066000 China

Dear Sir, I am Dr qing-li liu, from the Department of Urology of the First Hospital of Qinhuangdao in Qinhuangdao, Hebei Province, China. I write to present a case report of spermatic cord angiofibrolipoma.

关键词： Angiofibrolipoma spermatic cord

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Mechano-electric Effect of Hardened Cement Paste During Quasi-static Loading

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Journal of Wuhan University of Technology(Materials Science) 2001年第4期16卷 83-85页

作者：孙明清 Department of Engineering Mechanics Wuhan University of Technology Wuhan 430070 China

Mechano-electric effect of cement paste was investigated in this paper. As compressive stress was applied on the specimen, an electrical current was observed. The intensity of the electrical current increased with stress increasing, and decreased with stress decreasing. Different measurement methods were also discussed in this paper. This phenomenon was related to the electrokinetic phenomenon of solid/liquid interface in cement paste. The study on mechano-electric effect of hardened cement paste provides a new method for making smart concrete structures.

关键词： cement paste mechano-electric effect smart concrete

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Preparation of pyramid-SiNWs binary structure with Ag nanoparticles-assisted chemical etching

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Rare Metals 2019年第4期38卷 312-315页

作者： Zi-Long Zhang Bo Wang Yu Chen Yun-Hui Tang Xue-Mei Song qing-liu li Hui Yan Department of Materials Science and Engineering Beijing University of Technology

It is an important way to improve the efficiency of solar cells by using the special microstructures of *** this work,a pyramid-silicon nanowires (pyramidSiNWs) binary structure was prepared on the silicon surfaces with the metal-assisted chemical etching (MACE) *** electron microscope (SEM) was used to observe the micromorphology of the pyramid-SiNWs binary *** formation mechanism of the binary structure was *** role of Ag nanoparticles in MACE is considered to be the template and the *** optical reflectivity of the silicon surfaces was studied with ultraviolet-visible (UV-Vis) *** with the fiat silicon surface and the simple pyramidal structure,the silicon surfaces with the pyramid-SiNWs binary structure achieve a much lower reflectance in a wide range of *** effect of etching time as a parameter on the reflectivity was also discussed.

关键词： Ag nanoparticle Pyramid structure Silicon nanowires Binary structure Reflectance

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Prognostic role of C-reactive protein in prostate ：ancer： a systematic review and meta-analysis

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Asian Journal of Andrology 2014年第3期16卷 467-471页

作者： Zhu-qing liu li Chu Jue-Min Fang Xi Zhang Hua-Xin Zhao Yi-Jing Chen qing Xu Department of Medical Oncology Shanghai Tenth People's Hospital Tongji University School of Medicine Shanghai China.

Several studies have reported that C-reactive protein （CRP）, an inflammation biomarker, may be associated with the prognosis of prostate cancer （PCa）. The objective of this systematic review is to summarize the predictive role of CRP for survival in PCa as reported in previous studies. Related studies were identified, and evaluated for quality through multiple search strategies. Data was collected from studies comparing overall and cancer-specific survival （CSS） in patients with elevated CRP levels and those having lower levels. However, for progression-free survival （PFS）, data were collected according to the log of CRP. The hazard ratio （HR） and its 95% confidence interval （Cl） were used to assess the strength of associations. A total of nine studies （n = 1,497） were evaluated in this meta-analysis （five for overall survival （OS）, four for CSS and two for PFS）. For OS and PFS, the pooled HR of CRP was statistically significant at 1.51 （95% Cl, 1.28-1.79） and 1.50 （95% Cl, 1.25-1.81）, respectively. For CSS, the pooled HR was 1.91 （95% CI, 1.36-2.69） with higher CRP expression in PCa, which strongly indicates poorer survival in PCa. This study demonstrates that CRP may have a critical prognostic value in patients with prostatic cancer.

关键词： C-reactive protein meta-analysis prognosis prostate cancer

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Studies on plasma endothelin changes in varicocele patients

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Asian Journal of Andrology 1999年第3期1卷 159-160页

作者： qing-Quan XU Ji-Chuan ZHU Hu JIANG Xiao-Feng WANG Shu-Kun HOU qing-li liu Department of Urology, People’s Hospital, Beijing Medical University, Beijing 100011, China Department of Urology People's Hospital Beijing Medical University Beijing 100011 China.

Aim: To investigate the possible role endothelin may play in the pathogenesis of varicocele and varicocele-induced infer-tility. Methods: In varicocele patients, radioimmunoassay is used to determine the blood endothelin concentration inthe spermatic vein and the antecubital vein separately. Results: The blood endothelin concentration of the spermaticvein (66.8±23.5 pg/mL) was significantly higher (P lin concentration in the spermatic vein in Grade Ⅱ varicocele patients was not significantly different from thatin Grade Ⅲ patients ( P = 0.87). Conclusion: The higher blood endothelin level in the spermatic vein may indicate theinvolvement of endothelin in the pathophysiology of varicocele and varicocele-induced infertility. (Asian J Androl 1999Sep ; 1: 159 - 160)

关键词： endothelin varicocele infertility

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