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Rhamnolipids Induced by Glycerol Enhance Dibenzothiophene Biodegradation in Burkholderia sp. C3

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Engineering 2020年第5期6卷 533-540页

作者： Camila AOrtega Ramirez Abraham Kwan qing x.li Department of Molecular Biosciences and Bioengineering University of Hawaii at ManoaHonoluluHI 96822USA

In highly urbanized areas,pollution from anthropogenic activities has compromised the integrity of the land,decreasing soil availability for agricultural ***(DBT)is a heterocyclic aromatic hydrocarbon frequently found in urbanized areas,and is often used as a model chemical to study the microbial transformation of *** potential for human exposure and its health risk makes DBT a chemical of concern;thus,it needs to be environmentally *** utilized glycerol to stimulate Burkholderia sp.C3 in order to degrade DBT in respect to①DBT biodegradation kinetics,②bacterial growth,③rhamnolipid(RL)biosynthesis,and④RL *** an optimum glycerol-to-DBT molar ratio,the DBT biodegradation rate constants increased up to 18-fold and enhanced DBT biodegradation by 25%–30%at day 1 relative to cultivation with DBT *** enhancement was correlated with an increase in bacterial growth and RL *** studies revealed the enzymes involved in the upper and main steps of RL *** RL congeners Rha-C10-C10,Rha-Rha-C10-C10,Rha-Rha-C10-C12,and Rha-Rha-C12-C12 were identified in the medium supplemented with glycerol and DBT,whereas only Rha-C12-C12 was identified in cultures without glycerol or with RL *** studies indicated that glycerol enhances DBT biodegradation via increased RL synthesis and bacterial *** results warrant further studies of environmental biostimulation with glycerol to advance bioremediation technologies and increase soil availability for agricultural purposes.

关键词： Biodegradation Bioremediation Biosurfactant Biotransformation Glycerol Microbial metabolism Rhamnolipid

Laboratory studies of rice bran as a carbon source to stimulate indigenous microorganisms in oil reservoirs

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Petroleum Science 2016年第3期13卷 572-583页

作者： Chun-Mao Chen Jin-ling Wang Jung Bong Kim qing-Hong Wang Jing Wang Brandon A.Yoza qing x.li State Key Laboratory of Heavy Oil Processing China University of Petroleum Department of Molecular Biosciences and Bioengineering University of Hawaii at Manoa Department of Agro-Food Resources National Institute of Agricultural SciencesRural Development Administration Hawaii Natural Energy Institute University of Hawaii at Manoa

There is a great interest in developing cost-efficient nutrients to stimulate microorganisms in indigenous microbial enhanced oil recovery（IMEOR） *** the present study,the potential of rice bran as a carbon source for promoting IMEOR was investigated on a laboratory *** co-applications of rice bran,K2HPO4 and urea under optimized bio-stimulation conditions significantly increased the production of gases,acids and *** structure and diversity of microbial community greatly changed during the IMEOR process,in which Clostridium sp.,Acidobacteria sp.,Bacillus sp.,and Pseudomonas *** ***,acidification and emulsification due to microbial activities and interactions markedly improved the IMEOR *** study indicated that rice bran is a potential carbon source for IMEOR.

关键词： Rice bran Bio-stimulation Petroleum Microbial diversity Indigenous microbial enhanced oil recovery

Efficient ozonation of reverse osmosis concentrates from petroleum refinery wastewater using composite metal oxideloaded alumina

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Petroleum Science 2017年第3期14卷 605-615页

作者： Yu Chen Chun-Mao Chen Brandon A.Yoza qing x.li Shao-Hui Guo Ping Wang Shi-Jie Dong qing-Hong Wang State Key Laboratory of Petroleum Pollution Control Beijing Key Laboratory of Oil and Gas Pollution Control China University of Petroleum Hawaii Natural Energy Institute University of Hawaii at Manoa Department of Molecular Biosciences and Bioengineering University of Hawaii at Manoa

Novel Mn–Fe–Mg-and Mn–Fe–Ce-loaded alumina（Mn–Fe–Mg/Al2O3 and Mn–Fe–Ce/Al2O3） were developed to catalytically ozonate reverse osmosis concentrates generated from petroleum refinery wastewaters（PRW-ROC）. Highly dispersed 100–300-nm deposits of composite multivalent metal oxides of Mn（Mn^2＋）, Mn^3＋,and Mn^4＋, Fe（Fe^2＋）and Fe^3＋ and Mg（Mg^2＋）, or Ce（Ce^4＋） were achieved on Al2O3 supports. The developed Mn–Fe–Mg/Al2O3 and Mn–Fe–Ce/Al2O3 exhibited higher catalytic activity during the ozonation of PRW-ROC than Mn–Fe/Al2O3, Mn/Al2O-3, Fe/Al2O3, and Al2O3. Chemical oxygen demand removal by Mn–Fe–Mg/Al2O3-or Mn–Fe–Ce/Al2O3-catalyzed ozonation increased by 23.9% and23.2%, respectively, in comparison with single ***–Fe–Mg/Al2O3 and Mn–Fe–Ce/Al2O3 notably promoted áOH generation and áOH-mediated oxidation. This study demonstrated the potential use of composite metal oxide-loaded Al2O3 in advanced treatment of bio-recalcitrant wastewaters.

关键词： Petroleum refinery wastewater Reverseosmosis concentrate Catalytic ozonation Compositemetal oxide

Probiotic Lactobacillus brevis CLB3 prevents azoxymethane/dextran sulfate sodium-induced colon carcinogenesis in mice by reducing amino acid transport and IL-17A levels and repressing the IL-6/AKT/p-STAT3 signaling pathway

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Food Quality and Safety 2022年第4期6卷 664-677页

作者：钱伟祎张勇龙艳杨薇胡瑞李金萍冷燕刘欣洁李庆孝万向元魏珣 Zhongzhi International Institute of Agricultural Biosciences Shunde Graduate SchoolResearch Center of Biology and AgricultureUniversity of Science and Technology BeijingBeijingChina Beijing Beike Institute of Precision Medicine and Health Technology BeijingChina Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding Beijing International Science and Technology Cooperation Base of Bio-Tech BreedingBeijing Solidwill Sci-Tech Co.Ltd.BeijingChina College of Basic Science Tianjin Agricultural UniversityTianjinChina Department of Molecular Biosciences and Bioengineering University of Hawaii at ManoaHonoluluHawaiiUSA

Amino acid intake plays a crucial role in the Warburg effect of *** microbes can regulate intestinal amino acid ***,it is still unknown whether probiotic therapy can protect the host from intestinal tumor invasion by reducing amino acid *** in vitro methods,three acid-tolerant strains from fermented pickles were screened *** azoxymethane/dextran sulfate sodium-induced colon cancer models,we evaluated the therapeutic effects of Lactobacillus brevis CLB3,Lactobacillus plantarum xLP,and Lactobacillus johnsonii CM on model *** functional mechanisms were further explained through anatomy section,quantitative reverse transcription polymerase chain reaction(qRT-PCR),Western blot,and immunohistochemical staining analyses as well as database mining and gut *** Lactobacillus brevis(***)CLB3 treatment significantly improved the clinical signs and symptoms of colon cancer,alleviated colon damage,and inhibited colon carcinogenesis in *** addition,this treatment significantly increased gut cultivable Lactobacillus abundance,inhibited the expression and translation levels of the tumor metabolism-related solute carrier(SLC)amino acid transporter including SLC7A5 and SLC7A11,lowered circulating interleukin-6(IL-6)and interleukin-17A(IL-17A)levels,and improved the accumulation of tumor-infiltrating lymphocytes and cancer proliferation *** findings suggest that *** CLB3 can reduce amino acid transport,inhibit mammalian target of rapamycin(mTOR)signaling and enhance intestinal anti-tumor immune responses,which provides a potential targeting amino acid transporter strategy for preventing colorectal cancer.

关键词： Lactobacillus brevis CLB3 probiotic gut microbes colorectal cancer amino acid transporter

Selective photosynthesis of imines from biomass-derived aldehydes over Ni/TiO_(2)

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Rare Metals 2024年第3期43卷 1116-1124页

作者： Wu-lin li qing-qing Zhang lin Dong Wen-Jun Zhang li-li lin Zu-Peng Chen Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources International Innovation Center for Forest Chemicals and MaterialsCollege of Chemical EngineeringNanjing Forestry UniversityNanjing210037China Institute of Industrial Catalysis State Key Laboratory of Green Chemistry Synthesis TechnologyCollege of Chemical EngineeringZhejiang University of TechnologyHangzhou310014China

Photocatalytic reductive amination of biomass-derived aldehydes is a desirable way to selectively upgrade biomass into value-added nitrogen-containing chemicals under mild ***,it is challenging to produce imines in high selectivity because of the undesirable side reactions caused by the activity of functional ***,we demonstrate the highly reactive and selective production of imines from biomass derived aldehydes via the photocatalytic reductive amination,using a defective TiO_(2)supported nickel *** employment of methanol as the hydrogen donor and ammonia solution as the nitrogen source avoids the use of high-pressure H_(2)and expensive amines,rendering the current catalytic process safe,economical,and environmentally *** depth investigations attribute the improved separation and transfer of photogenerated charge carriers to the presence of oxygen vacancies and decorated Ni nanoparticles,thereby accelerating the production of imines from benzaldehyde amination(conversion,95.8%;seleetivity,95.2%).Furthermore,the developed system could be easily translated to the photocatalytic conversions of various biomass derived aldehydes,which provided an example of a cost-effective and sustainable approach for the valorization of biomass derived feedstocks.

关键词： Photocatalysis Biomass derivatives Imines Reduction amination Heterogeneous catalyst

lightweight Malicious Code Classification Method Based on Improved Squeeze Net

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Computers, Materials & Continua 2024年第1期78卷 551-567页

作者： li li Youran Kong qing Zhang School of Computer and Control Engineering Northeast Forestry UniversityHarbin150040China

With the growth of the Internet,more and more business is being done online,for example,online offices,online education and so *** this makes people’s lives more convenient,it also increases the risk of the network being attacked by malicious ***,it is important to identify malicious codes on computer systems ***,most of the existing malicious code detection methods have two problems:(1)The ability of the model to extract features is weak,resulting in poor model performance.(2)The large scale of model data leads to difficulties deploying on devices with limited ***,this paper proposes a lightweight malicious code identification model lightweight Malicious Code Classification Method Based on Improved SqueezeNet(LCMISNet).In this paper,the MFire lightweight feature extraction module is constructed by proposing a feature slicing module and a multi-size depthwise separable convolution *** feature slicing module reduces the number of parameters by grouping *** multi-size depthwise separable convolution module reduces the number of parameters and enhances the feature extraction capability by replacing the standard convolution with depthwise separable convolution with different convolution kernel *** addition,this paper also proposes a feature splicing module to connect the MFire lightweight feature extraction module based on the feature reuse and constructs the lightweight model *** malicious code recognition accuracy of LCMISNet on the BIG 2015 dataset and the Malimg dataset reaches 98.90% and 99.58%,*** proves that LCMISNet has a powerful malicious code recognition *** addition,compared with other network models,LCMISNet has better performance,and a lower number of parameters and computations.

关键词： lightweight neural network malicious code classification feature slicing feature splicing multi-size depthwise separable convolution

Numerical Study on Local Flow and Heat Transfer Characteristics of Supercritical CO_(2) in PCHE with Sinusoidal Channels

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Journal of Thermal Science 2024年第1期33卷 41-55页

作者： WENZhexi LV Yigao li qing WU Jinglin School of Energy Science and Engineering Central South UniversityChangsha 410083China

Local heat transfer and flow characteristics,which is crucial to the overall performance of supercritical CO_(2) recuperators,is rarely examined in details in reported *** this paper,the local heat transfer and flow characteristics of supercritical CO_(2) in sinusoidal channel printed circuit heat exchangers are numerically investigated under the working conditions of *** on the simplified physical model constituted by10 pitches,the variations of Re,heat flux,Nu,secondary flow and other relevant parameters along the flow direction are analyzed *** is further made between the high and low temperature recuperators(HTR and LTR).It's observed that the local heat transfer and flow characteristics vary greatly from the inlet to the outlet,especially in the *** of 127.5% and 61.7% can be observed on the cold side of the LTR for Re and heat transfer coefficient h, *** indicate that the temperature difference and heat transfer coefficient h should not be regarded as constant and the distribution of h should be carefully considered in the design of the *** complicated interactions among the varying thermophysical properties,buoyancy and the periodically changed centrifugal force are believed to be the key that shapes the flow ***,the changes of the wavy angle θ are found to have greater influence than the changes of mass flow rate in reshaping the flow field when θ>25°.Though gravity direction strongly affects the local heat transfer and flow characteristics,it's also found that the effects on the overall thermal-hydraulic performance are relatively *** the installation direction that yields a_y=g should better be avoided for the sinusoidal channel printed circuit heat exchanger.

关键词： printed circuit heat exchanger supercritical CO_(2) sinusoidal channel heat transfer recuperator

A GAN-EfficientNet-Based Traceability Method for Malicious Code Variant Families

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Computers, Materials & Continua 2024年第7期80卷 801-818页

作者： li li qing Zhang Youran Kong School of Computer and Control Engineering Northeast Forestry UniversityHarbin150040China

Due to the diversity and unpredictability of changes in malicious code,studying the traceability of variant families remains *** this paper,we propose a GAN-EfficientNetV2-based method for tracing families of malicious code *** method leverages the similarity in layouts and textures between images of malicious code variants from the same source and their original family of malicious code *** method includes a lightweight classifier and a *** classifier utilizes the enhanced EfficientNetV2 to categorize malicious code images and can be easily deployed on mobile,embedded,and other *** simulator utilizes an enhanced generative adversarial network to simulate different variants of malicious code and generates datasets to validate the model’s *** process helps identify model vulnerabilities and security risks,facilitating model enhancement and *** classifier achieves 98.61%and 97.59%accuracy on the MMCC dataset and Malevis dataset,*** simulator’s generated image of malicious code variants has an FID value of 155.44 and an IS value of 1.72±*** classifier’s accuracy for tracing the family of malicious code variants is as high as 90.29%,surpassing that of mainstream neural network *** meets the current demand for high generalization and anti-obfuscation abilities in malicious code classification models due to the rapid evolution of malicious code.

关键词： Malicious code variant traceability feature reuse lightweight neural networks code visualization attention mechanism

A Coupled Thermomechanical Crack Propagation Behavior of Brittle Materials by Peridynamic Differential Operator

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Computer Modeling in Engineering & Sciences 2024年第7期140卷 339-361页

作者： Tianyi li xin Gu qing Zhang College of Architectural Engineering Jiangsu Open UniversityNanjing210036China Department of Engineering Mechanics Hohai UniversityNanjing211100China

This study proposes a comprehensive,coupled thermomechanical model that replaces local spatial derivatives in classical differential thermomechanical equations with nonlocal integral forms derived from the peridynamic differential operator(PDDO),eliminating the need for calibration *** model employs a multi-rate ex.licit time integration scheme to handle varying time scales in multi-physics *** simulations conducted on granite and ceramic materials,this model demonstrates its *** successfully simulates thermal damage behavior in granite arising from incompatible mineral expansion and accurately calculates thermal crack propagation in ceramic slabs during *** account for material heterogeneity,the model utilizes the Shuffle algorithm andWeibull distribution,yielding results that align with numerical simulations and experimental *** coupled thermomechanical model shows great promise for analyzing intricate thermomechanical phenomena in brittle materials.

关键词： Peridynamic differential operator thermomechanical coupling heterogeneity numerical simulation