检索结果-南通市图书馆

Pooled Plasmid Sequencing Reveals the Relationship Between Mobile Genetic Elements and Antimicrobial Resistance Genes in Clinically Isolated Klebsiella pneumoniae

引用

Genomics, Proteomics & Bioinformatics 2020年第5期18卷 539-548页

作者： yan Jiang yanfei Wang Xiaoting hua Yue Qu Anton Y.Peleg Yunsong Yu Department of Infectious Diseases Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province Hangzhou 310016China Biomedicine Discovery Institute Department of MicrobiologyFaculty of MedicineNursing and Health SciencesMonash UniversityMelbourne 3800Australia Department of Infectious Diseases The Alfred Hospital and Central Clinical SchoolMonash UniversityMelbourne 3004Australia

Plasmids remain important microbial components mediating the horizontal gene transfer(HGT)and dissemination of antimicrobial *** systematically explore the relationship between mobile genetic elements(MGEs)and antimicrobial resistance genes(ARGs),a novel strategy using single-molecule real-time(SMRT)sequencing was *** approach was applied to pooled conjugative plasmids from clinically isolated multidrug-resistant(MDR)Klebsiella pneumoniae from a tertiary referral hospital over a 9-month *** conjugative plasmid pool was obtained from transconjugants that acquired antimicrobial resistance after plasmid conjugation with 53 clinical *** plasmid pool was then subjected to SMRT sequencing,and 82 assembled plasmid fragments were *** total,124 ARGs(responsible for resistance to b-lactam,fluoroquinolone,and aminoglycoside,among others)and 317 MGEs[including transposons(Tns),insertion sequences(ISs),and integrons]were derived from these *** of these ARGs were linked to MGEs,allowing for the establishment of a relationship network between MGEs and/or ARGs that can be used to describe the dissemination of resistance by mobile *** elements involved in resistance transposition were identified,including IS26,Tn3,IS903 B,ISEcp1,and *** the most predominant IS in the network,a typical IS26-mediated multi-copy composite transposition event was illustrated by tracing its flanking 8-bp target site duplications(TSDs).The landscape of the pooled plasmid sequences highlights the diversity and complexity of the relationship between MGEs and ARGs,underpinning the clinical value of dominant HGT profiles.

关键词： Horizontal transfer profile Transconjugant Single-molecule real time sequencing Insertion sequence Plasmid

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthesis and Investigation of Monoesters of Polymaleic Acids with Azo-Group

引用

Chemical Research in Chinese Universities 1997年第4期13卷 70-74页

作者： SU Feng yu LI yan TANG Jun TIAN yan qing ZHAO Ying ying ZHOU En le Polymer Physics Laboratory Changchun Institute of Applied Chemistry130022 Department of Chemistry Jilin UniversityChangchun130022

A series of amphiphilic polymers, monoesters of polymaleic acid containing azo side groups (PMAN A n Me) was synthesized by the reaction of alcohol(A n Me) with polymaleic anhydride (PMAN) for Langmuir Blodgett assembly. The effects of the length of flexible spacer in the side chain on the π A isotherms and LB films were investigated. The UV Vis and infrared linear dichroism spectra showed that the azo side groups were highly oriented in PMAN A 6Me LB film deposited on silica or CaF 2 substrate.

关键词： Monoesters of polymaleic acids LB film Synthesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

DIAGNOSIS AND MANAGEMENT OF ISOLATED PANCREATIC TUBERCULOSIS: EXPERIENCE OF 13 CASES

引用

Chinese Medical Sciences Journal 2007年第3期22卷 152-155页

作者： Chang-qing yan Jun-chao Guo Yu-pei Zhao Department of General Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 Department of General Surgery Second Hospital of Hebei Medical University Shijiazhuang 050000

Objective To analyze the diagnosis and treatment of pancreatic tuberculosis. Methods Retrospectively reviewed and summarized 13 pancreatic tuberculosis patients＇ clinical information, presentation, diagnostic methods, therapeutic approaches, and prognosis from 1958 to 2004 at Peking Union Medical College Hospital. Resuits All cases presented a wide series of symptoms, including fever in 6 cases, upper abdominal tenderness in 13, epigastric mass in 4, obstructive jaundice in 3, night sweat in 4, weight loss in 7, hypersplenoa＇ophy and hypersplenism in 1, and being complicated with tuberculosis of other organs in 3. One case was diagnosed by clinical symptoms and biopsy of lymph node, and only received anti-tubercular treatment. Others were diagnosed by intra-operative biopsy and anti-tubercular treatment, and got well without recurrent tuberculosis in pancreas and other organs during 6 months to 2 years of follow-up. The non-operative case presented extrahepatic portal hypertension. Conclusions Pancreatic tuberculosis may be considered in the patients with fever, abdominal tenderness, weight loss, and imaging evidence of regional pancreatic lesion. Efficacy of anti-tubercular agents and laparotomy for pancreatic tuberculosis is evident.

关键词： pancreatic tuberculosis diagnosis management

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Unraveling pharmaceuticals removal in a sulfur-driven autotrophic denitrification process:Performance,kinetics and mechanisms

引用

Chinese Chemical Letters 2023年第2期34卷 333-337页

作者： Jiying huang Yujian yan Yuyi Ou yanyan Jia Lianpeng Sun qing Zhao Hui Lu School of Environmental Science and Engineering Sun Yat-sen UniversityGuangzhou 510006China School of Ecology Sun Yat-sen UniversityGuangzhou 510006China Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology(Sun Yat-sen University) Guangzhou 510006China School of Civil Engineering Guangzhou UniversityGuangzhou 510006China

The removal of eight typical pharmaceuticals(Ph ACs)(i.e.,ibuprofen(IBU),ketoprofen(KET),diclofenac(DIC),sulfadiazine(SD),sulfamethoxazole(SMX),trimethoprim(TMP),ciprofloxacin(CIP)and enoxacin(ENO))in sulfur-driven autotrophic denitrification(Sd AD)process were firstly investigated via long-term operation of bioreactor coupled with batch *** results indicated that IBU and KET can be effectively removed(removal efficiency>50%)compared to other six Ph ACs in Sd AD *** was the primary removal route for IBU and KET with the specific biodegradation rates of 5.3±0.718.1±1.8μg g ^(-1)-VSS d ^(-1)at initial concentrations of 25-200μg/*** biotransformation intermediates of IBU and KET were examined,and the results indicated that IBU was biotransformed to three intermediates via hydroxylation and *** biotransformation could be initiated from the reduction of the keto group following with a series of oxidation/reduction reactions,and five intermediates of KET were observed in this *** microbial community composition in the system was markedly shifted when long-term exposure to Ph ***,the functional microbes(e.g.,genus Thiobacillus)showed high tolerance to Ph ACs,resulting in the high efficiency for Ph ACs,N and S removal during long-term Sd AD reactor *** findings provide better insight into Ph ACs removal in Sd AD process,especially IBU and KET,and open up an innovative opportunity for the treatment of Ph ACs-laden wastewater using sulfur-mediated biological process.

关键词： Pharmaceuticals(PhACs) Ibuprofen(IBU) Ketoprofen(KET) Biodegradation Sulfur-driven autotrophic denitrification process

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

杭白菊及其F1代单株头状花序的营养成分比较分析

引用

现代食品科技 2024年第6期40卷 53-65页

作者：吕金李凤马天贵周厚高王凤兰陈砚罗红辉伍青仲恺农业工程学院园艺园林学院广东省普通高校热带亚热带花卉与园林植物重点实验室广东广州510225 息烽都市现代农业扶贫开发投资有限公司贵州贵阳551100 仁善田园(广东)农业科技发展有限公司广东云浮527329

通过液相质谱联用技术对杭白菊及其F1代优选单株的花朵(头状花序)进行营养成分比较分析。结果表明,杭白菊、杭F1-17和杭F1-22中分别检测到502、505和496个代谢物,三者共有代谢物309个。结合差异倍数值FC≥1.5和≤0.8筛选杭白菊vs杭F1-17... 详细信息

通过液相质谱联用技术对杭白菊及其F1代优选单株的花朵(头状花序)进行营养成分比较分析。结果表明,杭白菊、杭F1-17和杭F1-22中分别检测到502、505和496个代谢物,三者共有代谢物309个。结合差异倍数值FC≥1.5和≤0.8筛选杭白菊vs杭F1-17和vs杭F1-22的差异代谢物中分别有135和206种上调、144和154种下调,其中,杭F1-17和杭F1-22的黄酮类代谢物山奈酚(FC为16.77和18.69)、木犀草素(FC为7.35和3.74)、异鼠李素(FC为3.21和6.38)、人体必须氨基酸之一的甲硫氨酸(FC为103.22和67.71)、主要有机酸苹果酸(FC为306.73和2.76)和柠檬酸(FC为4.82和12.16)、菊花主要保健成分绿原酸(FC为1.53和3.11)等物质的相对含量均明显上调。此外,与母本杭白菊相比,杭F1-17特有熊果酸、维生素A和K3等成分,杭F1-22特有鼠尾草酚、杨梅素、维生素B1等成分。以上研究表明杭白菊F1代优选单株中的主要营养成分相对含量明显较母本高,同时在F1代单株中还检测到特有营养成分,且杭F1-17头状花序的托桂特性也为开发观食两用的优良茶用菊新品种提供了参考。

关键词：杭白菊 F1代单株茶用菊营养成分液相质谱

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

工程装备液压系统维修模拟实训台设计

引用

机电产品开发与创新 2024年第2期37卷 176-179页

作者：洪广学李彦革周春华河北冀川实业总公司河北石家庄050200 陆军工程大学野战工程学院江苏南京210007

分析了工程装备液压系统的典型故障特点、故障检测维修现状与维修保障需求,应用嵌入式技术、故障检测技术、虚拟仪器技术、模块化技术等,设计实现了工程装备液压系统维修模拟实训台。通过装备液压系统工作原理展示、典型故障虚实模拟、... 详细信息

分析了工程装备液压系统的典型故障特点、故障检测维修现状与维修保障需求,应用嵌入式技术、故障检测技术、虚拟仪器技术、模块化技术等,设计实现了工程装备液压系统维修模拟实训台。通过装备液压系统工作原理展示、典型故障虚实模拟、人机交互手动实践多种形式,实现了既可循序渐进又可灵活跳跃地解析和仿真液压系统故障的发生机理、发展过程与表现特征,利用可视化手段指导和辅助培训对象进行液压系统故障的检测诊断与排除维修,为维修人员的教育和培训提供了一个有效的解决途径。

关键词：液压系统故障模拟虚拟维修实训台

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microarray analysis of Escherichia coli0157:H7

引用

World Journal of Gastroenterology 2005年第37期11卷 5811-5815页

作者： Hui-Ying Jin Kai-hua Tao Yue-Xi Li Fa-qing Li Su-Qin Li huadong Research Institute for Medicine and Biotechnics Nanjing 210002 Jiangsu Province China

AIM： To establish the rapid, specific, and sensitive method for detecting O157：H7 with DNA microchips. METHODS： Specific oligonucleotide probes （26-28 nt） of bacterial antigenic and virulent genes of E. coliO157：H7 and other related pathogen genes were pre-synthesized and immobilized on a solid support to make microchips. The four genes encoding O157 somatic antigen （rfbE）, H7 flagellar antigen （fliC） and toxins （SLT1, SLT2） were monitored by multiplex PCR with four pairs of specific primers. Fluorescence-Cy3 labeled samples for hybridization were generated by PCR with Cy3-1abeled single prime. Hybridization was performed for 60 min at 45 ℃. Microchip images were taken using a confocal fluorescent scanner. RESULTS： Twelve different bacterial strains were detected with various combinations of four virulent genes. All the O157：H7 strains yielded positive results by multiplex PCR. The size of the PCR products generated with these primers varied from 210 to 678 bp. All the rfbE/fliC/SLT1/SLT2 probes specifically recognized Cy3-1abeled fluorescent samples from O157：H7 strains, or strains containing O157 and H7 genes. No cross hybridization of O157：H7 fluorescent samples occurred in other probes. Non-O157：H7 pathogens failed to yield any signal under comparable conditions. If the Cy3-1abeled fluorescent product of O157 single PCR was diluted 50-fold, no signal was found in agarose gel electrophoresis, but a positive signal was found in microarray hybridization. CONCLUSION： Microarray analysis of O157：H7 is a rapid, specific, and efficient method for identification and detection of bacterial pathogens.

关键词： Microarray Multiplex PCR, Escherichia coli O157:H7 Shiga-like toxin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

血小板计数与血沉检测在社区结核病筛查中的价值

引用

中国实用医药 2024年第19期19卷 88-91页

作者：金丽华严聪聪王军伟浙江省天台县人民医院感染科 317200

目的探讨血小板计数与血沉检测在社区结核病筛查中的价值。方法对县域医共体内公共卫生检查数据进行分析,抽取血沉(mm/h)+血小板计数(万/mm^(3))≥80者,结合既往史、现病史及胸部X线资料进行分析,观察结核病筛查结果。结果共筛查公... 详细信息

目的探讨血小板计数与血沉检测在社区结核病筛查中的价值。方法对县域医共体内公共卫生检查数据进行分析,抽取血沉(mm/h)+血小板计数(万/mm^(3))≥80者,结合既往史、现病史及胸部X线资料进行分析,观察结核病筛查结果。结果共筛查公共卫生健康体检者111489人,男性56721人,女性54768人,共筛查到血沉(mm/h)+血小板计数(万/mm^(3))≥80者共1589例,占比1.43%。其中<18岁909例,人数比例最高,且以急性炎症性疾病居多;成人(≥18岁)680例。成人中,年龄≥80岁109例(结核病71例,占65.14%);70~79岁195例(结核病65例,占33.33%);60~69岁178例(结核病37例,占20.79%);50~59岁106例(结核病17例,占16.04%);30~49岁76例(结核病6例,占7.89%);18~29岁16例(结核病3例,占18.75%),随着年龄增大,结核病检出率呈上升趋势,各年龄段比较有显著性差异(P<0.01)。男性286例(结核病101例,占35.31%),女性394例(结核病98例,占24.87%),男性的检出率明显高于女性,有显著性差异(P<0.01)。结论血小板计数与血沉在社区结核病筛查中有一定价值,血沉(mm/h)+血小板计数(万/mm^(3))≥80者结核病可能性大,尤其是60岁以上患者价值更大。

关键词：血小板计数血沉结核病筛查县域医共体

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Properties of fiber reinforced plaster molds for investment casting

引用

China Foundry 2020年第5期17卷 332-340页

作者： Jie Ren Xiang Chen yan-xiang Li Yuan Liu hua-wei Zhang School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China Key Laboratory for Advanced Materials Processing Technology Tsinghua UniversityBeijing 100084China

In order to improve the performance of plaster molds for investment casting,a diverse content of glass fiber and polypropylene(PP)fiber was incorporated into the slurry for the preparation of a fiber-reinforced *** green and fired bending strengths,thermal expansion properties,permeability,and thermal shock resistance of the mold were examined,and the scanning electron microscope(SEM)with energy dispersive spectrometer(EDS)was applied for the observation of fracture *** appropriate content,the introduction of glass fiber was proved to increase the green bending strength and fired bending strength,restrain the thermal expansion and improve the thermal shock resistance of the mold,while the polypropylene fiber added was able to raise the green bending strength and the permeability,reduce the thermal expansion and heighten the thermal shock resistance as well,though the fired bending strength would be weakened *** distributed fibers were capable of enhancing the mechanical properties of the matrix,but agglomerations and bundles of fibers resulting from excessive addition had a negative ***,it was also manifested that micropores left by ablative polypropylene fibers could improve the permeability and reduce the thermal expansion of the mold,and the fired bending strength would be decreased slightly by the deterioration of continuous *** different ratios of hybrid fiber were employed in plaster molds,which can meet altered requirement of *** samples modified with hybrid fiber possessed lower thermal deformation,higher air permeability,and better resistance of thermal shock,while the mechanical strength was equal to the fiber free sample or slightly increased.

关键词： plaster mold investment casting fiber-reinforced bending strength thermal expansion permeability thermal shock resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genome-wide identification of key regulatory lncRNAs in esophageal cancer metastasis

引用

Signal Transduction and Targeted Therapy 2021年第3期6卷 621-623页

作者： Wen Wen Xu Can-Can Zheng Qian Zuo Jun-Qi Li Pan Hong yan-Ru Qin Xin-Yuan Guan qing-Yu He hua-Xin Liao Bin Li MOE Key Laboratory of Tumor Molecular Biology and Guangdong Provincial Key Laboratory of Bioengineering Medicine National Engineering Research Center of Genetic MedicineInstitute of BiomedicineCollege of Life Science and TechnologyJinan UniversityGuangzhouChina MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health EngineeringCollege of Life Science and TechnologyJinan UniversityGuangzhouChina State Key Laboratory of Esophageal Cancer Prevention and Treatment Department of Clinical OncologyFirst Affiliated HospitalZhengzhou UniversityZhengzhouChina Department of Clinical Oncology Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong Kong SARChina

Dear Editor,Metastasis leads to a poor prognosis of patients with esophageal squamous cell carcinoma(ESCC).1-3 but the study on cancer metastasis has been hampered by a lack of reliable cell and animal *** identification and functional validation of metastasis-associated long non-coding RNAs(lncRNAs)and microRNAs(miRNAs),as well as-their interactions in ESCC are urgently needed.

关键词： metastasis esophageal cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：