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Gene cloning,protein expression,and enzymatic characterization of a double-stranded RNA degrading enzyme in Apolygus lucorum

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Insect Science 2024年第1期31卷 119-133页

作者： jie-Yu Zhang jing Zhao Keyan Zhu-Salzman qin-qin ji Yi-Ping jiang Liu-Bin Xiao De-jin Xu Guang-Chun Xu Lin-Quan Ge Yong-An Tan College of Plant Protection Yangzhou UniversityYangzhouJiangsu ProvinceChina Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjingJiangsu ProvinceChina Department of Entomology Texas A&M UniversityCollege StationTXUSA Taizhou Customs of the People's Republic of China TaizhouJiangsu ProvinceChina

RNA interference(RNAi)is a powerful tool that post-transcriptionally silences target genes in eukaryotic ***,silencing efficacy varies greatly among different insect ***,we met with little success when attempting to knock down genes in the mirid bug Apolygus lucorum via dsRNA *** disappearance of double-stranded RNA(dsRNA)could be a potential factor that restricts RNAi ***,we found that dsRNA can be degraded in midgut fluids,and a dsRNase of ***(AldsRNase)was identified and *** alignment indicated that its 6 key amino acid residues and the Mg2+-binding site were similar to those of other insects’*** signal peptide and endonuclease non-specific domain shared high sequence identity with the brown-winged green stinkbug Plautia stali *** showed high salivary gland and midgut expression and was continuously expressed through the whole life cycle,with peaks at the 4th instar ecdysis in the whole *** purified AldsRNase protein obtained by heterologously expressed can rapidly degrade *** comparing the substrate specificity of AldsRNase,3 specific substrates(dsRNA,small interfering RNA,and dsDNA)were all degraded,and the most efficient degradation is ***,immunofluorescence revealed that AldsRNase was expressed in the cytoplasm of midgut *** cloning and functional study of AldsRNase,the enzyme activity and substrate specificity of the recombinant protein,as well as the subcellular localization of nuclease,the reason for the disappearance of dsRNA was explained,which was useful in improving RNAi efficiency in *** and related species.

关键词： Apolygus lucorum cloning dsRNase enzymatic activity protein expression

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A Tri-mode of Mock-Walker Cells

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Advances in Atmospheric Sciences 2024年第4期41卷 671-679页

作者： Han qin ji NIE Zhiyong MENG Laboratory for Climate and Ocean-Atmosphere Studies Department of Atmospheric and Oceanic SciencesSchool of PhysicsPeking UniversityBeijing 100871China China Meteorological Administration Tornado Key Laboratory Foshan 528061China

This work uses cloud-resolving simulations to study mock-Walker cells driven by a specified sea surface temperature(SST).The associated precipitation in the mock-Walker cells exhibits three different modes,including a single peak of precipitation over the SST maximum(mode 1),symmetric double peaks of precipitation straddling the SST maximum(mode 2),and a single peak of precipitation on one side of the SST maximum(mode 3).The three modes are caused by three distinct convective activity center migration *** indicate that the virtual effect of water vapor plays an important role in differentiating the three *** the SST gradient is large,the virtual effect may be strong enough to overcome the temperature effect,generating a low-level low-pressure anomaly below the ascending branch of the Walker cell off the *** results here highlight the importance of the virtual effect of water vapor and its interaction with convection and large-scale circulation in the Walker circulation.

关键词： Walker circulation cloud-resolving simulation virtual effect

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Role of catalyst surface-active sites in the hydrogenation ofα,β-unsaturated aldehyde

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Frontiers of Chemical Science and Engineering 2024年第6期18卷 41-69页

作者： Haixiang Shi Tongming Su Zuzeng qin Hongbing ji School of Chemistry and Chemical Engineering Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification TechnologyGuangxi UniversityNanning 530004China Zhejiang Green Petrochemical and Light Hydrocarbon Transformation Research Institute Zhejiang University of TechnologyHangzhou 310014China

As an important technology in fine chemical production,the selective hydrogenation ofα,β-unsaturated aldehydes has attracted much attention in recent *** the process ofα,β-unsaturated aldehyde hydrogenation,a conjugated system is formed between>C=CC=O,leading to hydrogenation at both ends of the conjugated system,which competes with each other and results in more complex ***,improving the reaction selectivity is also difficult in industrial ***,many researchers have reported that surface-active sites on catalysts play a crucial role inα,β-unsaturated aldehyde *** review attempts to summarize recent advances in understanding the effects of surface-active sites(SASs)over metal catalysts for enhancing the process of *** construction strategies and roles of SASs for hydrogenation catalysts are *** attention has been given to the adsorption configuration and transformation mechanism ofα,β-unsaturated aldehydes on catalysts,which contributes to understanding the relationship between SASs and hydrogenation *** addition,recent advances in metal-supported catalysts for the selective hydrogenation ofα,β-unsaturated aldehydes to understand the role of SASs in hydrogenation are briefly ***,the opportunities and challenges will be highlighted for the future development of the precise construction of SASs.

关键词： α,β-unsaturatedd aldehydes hydrogenation active site cinnamaldehyde

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Experimental and numerical investigation of the impacts of rotor tip-rake on excitation forces of pump-jet propulsors

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Journal of Hydrodynamics 2024年第1期36卷 142-157页

作者： Xue-qin ji Xiao-song Zhang Chen-jun Yang Xiao-qian Dong State Key Laboratory of Ocean Engineering Marine Numerical Experiment CentreSchool of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China CSSC Shanghai Marine Energy Saving Technology Co. Ltd.Shanghai200011China China Ship Scientific Research Center Shanghai Branch Shanghai200011China

The tip-clearance flow in a pump-jet propulsor exerts great impacts on the fluctuating pressures and resultant unsteady forces,which are important sources of structural vibrations and radiated noise *** blade geometry close to the tip is an important factor determining the vortex strength in the tip-clearance *** the open-water condition,the effects of raking the rotor tips on the duct-surface fluctuating pressures and the resultant unsteady forces acting on different components of the propulsor are investigated via physical model experiments and the numerical solution of Reynolds-averaged Navier-Stokes(RANS)equations coupled with the SST k-ωturbulence *** measured and simulated results of hydrodynamic pressures are consistent to each other,and the simulated flows help better understand why the fluctuating pressures change with the tip *** strong fluctuations of duct-surface pressures are caused by intensive tip separation *** duct-surface pressure fluctuations are effectively reduced by using the rake distribution near the tip towards blade back side and,for the combination of the five-bladed rotor and the seven-bladed stator,the resultant unsteady horizontal(and vertical)forces acting on the duct and stator are also reduced;while increasing rake leads to negative effect on pressure fluctuations and unsteady horizontal(and vertical)forces acting on all the components of the propulsor.

关键词： Pump-jet tip-clearance tip-rake fluctuating pressures excitation forces

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Resolution Enhancement of SOHO/MDI Magnetograms

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Research in Astronomy and Astrophysics 2024年第6期24卷 358-367页

作者： Ying qin Kai-Fan ji Hui Liu Xiao-Guang Yu Yunnan Observatories Chinese Academy of SciencesKunming 650011China University of Chinese Academy of Sciences Beijing 100049China Yunnan Key Laboratory of Solar Physics and Space Science Kunming 650216China Key Laboratory for the Structure and Evolution of Celestial Objects Chinese Academy of SciencesKunming 650216China

Research on the solar magnetic field and its effects on solar dynamo mechanisms and space weather events has benefited from the continual improvements in instrument resolution and measurement *** augmentation and assimilation of historical observational data timelines also play a significant role in understanding the patterns of solar magnetic field *** the realm of astronomical data processing,super-resolution(SR)reconstruction refers to the process of using a substantial corpus of training data to learn the nonlinear mapping between low-resolution(LR)and high-resolution(HR)images,thereby achieving higherresolution astronomical *** paper is an application study in high-dimensional nonlinear *** learning models were employed to perform SR modeling on SOHO/MDI magnetograms and SDO/HMI magnetograms,thus reliably achieving resolution enhancement of full-disk SOHO/MDI magnetograms and enhancing the image resolution to obtain more detailed *** this study,a data set comprising 9717pairs of data from 2010 April to 2011 February was used as the training set,1332 pairs from 2011 March were used as the validation set and 1034 pairs from 2011 April were used as the test *** data preprocessing,we randomly cropped 128×128 sub-images as the LR cases from the full-disk MDI magnetograms,and the corresponding 512×512 sub-images as HR ones from the HMI full-disk magnetograms for model *** tests conducted have shown that the study successfully produced reliable 4×SR reconstruction of full-disk MDI *** MESR model's results(0.911)were highly correlated with the target HMI magnetographs as indicated by the correlation coefficient ***,the method achieved the best PSNR,SSIM,MAE and RMSE values,indicating that the MESR model can effectively reconstruct magnetograms.

关键词： magnetic fields techniques:image processing virtual observatory tools Sun:magnetic fields

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In-Vehicle Network Injection Attacks Detection Based on Feature Selection and Classification

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Automotive Innovation 2024年第1期7卷 138-149页

作者： Haojie ji Liyong Wang Hongmao qin Yinghui Wang Junjie Zhang Biao Chen Key Laboratory of Modern Measurement and Control Technology Ministry of EducationBeijing Information Science and Technology UniversityBeijing 100192China State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body College of Mechanical and Vehicle EngineeringHunan UniversityChangsha 410082China School of Transportation Science and Engineering Beihang UniversityBeijing 100191China Hefei Innovation Research Institute Beihang UniversityHefei 230012China

Detecting abnormal data generated from cyberattacks has emerged as a crucial approach for identifying security threats within in-vehicle *** transmission of information through in-vehicle networks needs to follow specific data for-mats and communication protocols ***,statistical algorithms are employed to learn these variation rules and facilitate the identification of abnormal ***,the effectiveness of anomaly detection outcomes often falls short when confronted with highly deceptive in-vehicle network *** this study,seven representative classification algorithms are selected to detect common in-vehicle network attacks,and a comparative analysis is employed to identify the most suitable and favorable detection *** consideration of the communication protocol characteristics of in-vehicle networks,an optimal convolutional neural network(CNN)detection algorithm is proposed that uses data field characteristics and classifier selection,and its comprehensive performance is *** addition,the concept of Hamming distance between two adjacent packets within the in-vehicle network is introduced,enabling the proposal of an enhanced CNN algorithm that achieves robust detection of challenging-to-identify abnormal *** paper also presents the proposed CNN classifica-tion algorithm that effectively addresses the issue of high false negative rate(FNR)in abnormal data detection based on the timestamp feature of data *** experimental results validate the efficacy of the proposed abnormal data detection algorithm,highlighting its strong detection performance and its potential to provide an effective solution for safeguarding the security of in-vehicle network information.

关键词： Classification algorithm Anomaly detection In-vehicle network Feature extraction Injecting attack

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Data-driven casting defect prediction model for sand casting based on random forest classification algorithm

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China Foundry 2024年第2期21卷 137-146页

作者： Bang Guan Dong-hong Wang Da Shu Shou-qin Zhu Xiao-yuan ji Bao-de Sun Shanghai Key Lab of Advanced High-Temperature Materials and Precision Forming School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China State Key Lab of Metal Matrix Composites Shanghai Jiao Tong UniversityShanghai 200240China Hefei Casting and Forging Factory of Anhui Heli Co. LtdHefei 230000China State Key Laboratory of Materials Processing and Die&Mould Technology Huazhong University of Science and TechnologyWuhan 430074China

The complex sand-casting process combined with the interactions between process parameters makes it difficult to control the casting quality,resulting in a high scrap rate.A strategy based on a data-driven model was proposed to reduce casting defects and improve production efficiency,which includes the random forest(RF)classification model,the feature importance analysis,and the process parameters optimization with Monte Carlo *** collected data includes four types of defects and corresponding process parameters were used to construct the RF *** results show a recall rate above 90% for all *** Gini Index was used to assess the importance of the process parameters in the formation of various defects in the RF ***,the classification model was applied to different production conditions for quality *** the case of process parameters optimization for gas porosity defects,this model serves as an experimental process in the Monte Carlo method to estimate a better temperature *** prediction model,when applied to the factory,greatly improved the efficiency of defect *** show that the scrap rate decreased from 10.16% to 6.68%.

关键词： sand casting process data-driven method classification model quality prediction feature importance

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Theoretical and experimental study of a bi-stable piezoelectric energy harvester under hybrid galloping and band-limited random excitations

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Applied Mathematics and Mechanics(English Edition) 2024年第3期45卷 461-478页

作者： Haitao LI Tianyu ZHENG Weiyang qin Ruilan TIAN Hu DING J.C.ji Liqun CHEN Department of Engineering Mechanics North University of ChinaTaiyuan 030051China Department of Engineering Mechanics Northwestern Polytechnical UniversityXi'an 710072China Department of Engineering Mechanics Shijiazhuang Tiedao UniversityShijiazhuang 050043China Shanghai Key Laboratory of Mechanics in Energy Engineering Shanghai Frontier Science Center of MechnoinformaticsShanghai Institute of Applied Mathematics and MechanicsSchool of Mechanics and Engineering ScienceShanghai UniversityShanghai 200072China School of Mechanical and Mechatronic Engineering University of Technology SydneyNew South Wales 2007Australia

In the practical environment,it is very common for the simultaneous occurrence of base excitation and *** the combined energy of vibration and wind with a single energy harvesting structure is *** this purpose,the effects of the wind speed and random excitation level are investigated with the stochastic averaging method(SAM)based on the energy *** results of the analytical prediction are verified with the Monte-Carlo method(MCM).The numerical simulation shows that the introduction of wind can reduce the critical excitation level for triggering an inter-well jump and make a bi-stable energy harvester(BEH)realize the performance enhancement for a weak base ***,as the strength of the wind increases to a particular level,the influence of the random base excitation on the dynamic responses is weakened,and the system exhibits a periodic galloping response.A comparison between a BEH and a linear energy harvester(LEH)indicates that the BEH demonstrates inferior performance for high-speed *** experiments are conducted to investigate the validity of the theoretical prediction and numerical *** experimental findings also show that strong random excitation is favorable for the BEH in the range of low wind ***,as the speed of the incoming wind is up to a particular level,the disadvantage of the BEH becomes clear and evident.

关键词： bi-stability inter-well response galloping band-limited random

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Graphene photodetectors integrated with silicon and perovskite quantum dots

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MICROSYSTEMS & NANOENGINEERING 2024年第1期10卷 1-13页

作者： Abbas, Kashif ji, Peirui Ullah, Naveed Shafique, Shareen Zhang, Ze Ameer, Muhammad Faizan qin, Shenghan Yang, Shuming Xi An jiao Tong Univ State Key Lab Mfg Syst Engn Xian 710049 Peoples R China Ningbo Univ Ningbo Collaborat Innovat Ctr Nonlinear Calam Syst Sch Phys Sci & Technol Dept Microelect Sci & EngnLab Clean Energy Storag Ningbo 315211 Peoples R China

Photodetectors (PDs) play a crucial role in imaging, sensing, communication systems, etc. Graphene (Gr), a leading two-dimensional material, has demonstrated significant potential for photodetection in recent years. However, its relatively weak interaction with light poses challenges for practical applications. The integration of silicon (Si) and perovskite quantum dots (PQDs) has opened new avenues for Gr in the realm of next-generation optoelectronics. This review provides a comprehensive investigation of Gr/Si Schottky junction PDs and Gr/PQD hybrid PDs as well as their heterostructures. The operating principles, design, fabrication, optimization strategies, and typical applications of these devices are studied and summarized. Through these discussions, we aim to illuminate the current challenges and offer insights into future directions in this rapidly evolving field.

关键词： SCHOTTKY PHOTODETECTOR PHOTORESPONSE

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Kinetic model of vibration screening for granular materials based on biological neural network

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Particuology 2024年第5期88卷 98-106页

作者： Zhan Zhao Yan Zhang Fang qin Mingzhi jin School of Agricultural Engineering Jiangsu UniversityZhenjiang212013China Shandong Academy of Agricultural Machinery Sciences Ji'nan250100China

The kinetic model is the theoretical basis for optimizing the structure and operation performance of vibration screening *** this paper,a biological neurodynamic equation and neural connections were established according to the motion and interaction properties of the material under vibration *** material feeding to the screen and the material passing through apertures were considered as excitatory and inhibitory inputs,respectively,and the generated stable neural activity landscape was used to describe the material distribution on the 2D screen *** dynamic process of material vibration screening was simulated using discrete element method(DEM).By comparing the similarity between the material distribution established using biological neural network(BNN)and that obtained using DEM simulation,the optimum coefficients of BNN model under a certain screening parameter were determined,that is,one relationship between the BNN model coefficients and the screening operation parameters was *** screening parameters were randomly selected,and the corresponding relationships were established as a ***,with straw/grain ratio,aperture diameter,inclination angle,vibration strength in normal and tangential directions as inputs,five independent adaptive neuro-fuzzy inference systems(ANFIS)were established to predict the optimum BNN model coefficients,*** training results indicated that ANFIS models had good stability and *** flexibility and adaptability of the proposed BNN method was demonstrated by modeling material distribution under complex feeding conditions such as multiple regions and non-uniform rate.

关键词： Kinetic model Material distribution Vibration screening Biological neural network DEM simulation Adaptive neuro-fuzzy inference systems

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