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How incubation temperature affects hatchling performance in reptiles: an integrative insight based on plasticity in metabolic enzyme

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Current Zoology 2024年第2期70卷 195-203页

作者： Dan-yang Wu Xing-Zhi Han Teng li Bao-Jun Sun Xiao-Yan qin College of life and Environment Sciences Minzu University of ChinaBeijing 100081China Key Laboratory of Animal Ecology and Conservational Biology Institute of ZoologyChinese Academy of SciencesBeijing 10011China College of Wildlife and Protected Area Northeast Forestry UniversityHarbin 150040China College of Resources and Environmental Sciences Nanjing Agricultural UniversityNanjing 210095China

Evaluating the effects of temperature variations on animals plays an important role in understanding the threat of climate *** effects of developmental temperature on offspring performance are critical in evaluating the effects of warming temperatures on the fitness of ovipa-rous species,but the physiological and biochemical basis of this developmental plasticity is largely *** this study,we incubated eggs of the turtle Pelodiscus sinensis at low(24℃),medium(28℃),and high(32℃)temperatures,and evaluated the effects of developmental temperature on offspring fitness,and metabolic enzymes in the neck and limb muscles of *** hatchlings from eggs incubated at the medium temperature showed better fitness-related performance(righting response and swimming capacity)and higher activities of metabolic enzymes(hexokinase,HK;lactate dehydrogenase,LDH)than hatchlings from the eggs incubated at high or low *** addition,the swimming speed and righting response were significantly correlated with the HK activities in limb(swimming speed)and neck(righting response)muscles,suggesting that the developmental plasticity of energy metabolic pathway might play a role in determining the way incubation temperature affects offspring *** the fitness-related performance and the activities of metabolic enzymes,we predict that the P sinensis from high latitude would not face the detrimental effects of climate warming until the average nest temperatures reach 32℃.

关键词： developmental plasticity energetic metabolism enzyme activity hatchling turtle.

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Hypermethylation in H3K9me3 regions characterizes the centenarian methylomes in healthy aging

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National Science Review 2023年第6期10卷 60-63页

作者： Fu-Hui Xiao Hao-Tian Wang Xiao-Qiong Chen Ming-Xia Ge Dongjing Yan Xing-li yang li-qin yang Rong lin Rong-Hui Guo Wen Zhang Nelson Leung-Sang Tang Yonghan He Jumin Zhou Wang-Wei Cai qing-Peng Kong State Key Laboratory of Genetic Resources and Evolution Key Laboratory of Healthy Aging Research of Yunnan Province Kunming Key Laboratory of Healthy Aging Study Kunming Institute of ZoologyChinese Academy of Sciences KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases Kunming College of life Science University of Chinese Academy of Sciences Department of Biochemistry and Molecular Biology Hainan Medical College Department of Biology Hainan Medical College Department of Chemical Pathology and Laboratory for Genetics of Disease Susceptibility Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences Faculty of Medicine The Chinese University of Hong Kong Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences Key Laboratory of Healthy Aging Research of Yunnan Province Kunming Institute of Zoology CAS Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences

Centenarians,as excellent examples of extreme yet successful aging,not only display a much longer lifespan but can also delay or even escape major agerelated diseases,such as cardiovascular disorders,neurodegenerative diseases,and cancer [1].Studies have suggested that genetic factors account for 20%-30%of human longevity [2];however,only a handful of longevity-associated genes/mutations have been observed in long-lived individuals,even with the use of genome-wide scanning/sequencing assays [3,4].In contrast,evidence has shown the crucial roles of epigenetic alternations in aging and age-related diseases [5],suggesting that epigenetic modifications play central roles in human healthy ***,a comprehensive analysis of the epigenomic landscape of centenarians is urgently needed.

关键词： LONGEVITY DNA

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Simulation of Dynamic Recrystallization in 7075 Aluminum Alloy Using Cellular Automaton

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Journal of Wuhan University of Technology(Materials Science) 2024年第2期39卷 425-435页

作者：赵晓东 SHI Dongxing 李亚杰 qin Fengming CHU Zhibing yang Xiaorong School of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024China

The evolution of microstructure during hot deformation is key to achieving good mechanical properties in aluminum *** have developed a cellular automaton(CA) based model to simulate the microstructural evolution in 7075 aluminum alloy during hot *** compression tests were conducted to obtain material parameters for 7075 aluminum alloy,leading to the establishment of models for dislocation density,nucleation of recrystallized grains,and grain *** these aspects with grain topological deformation,our CA model effectively predicts flow stress,dynamic recrystallization(DRX) volume fraction,and average grain size under diverse deformation conditions.A systematic comparison was made between electron back scattered diffraction(EBSD) maps and CA model simulated under different deformation temperatures(573 to 723 K),strain rates(0.001 to 1 s^(-1)),and strain amounts(30% to 70%).These analyses indicate that large strain,high temperature,and low strain rate facilitate dynamic recrystallization and grain *** results from the CA model show good accuracy and predictive capability,with experimental error within 10%.

关键词： cellular automaton dynamic recrystallization 7075 aluminum alloy hot compression

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Effect of streamlined nose length on aerodynamic performance of high-speed train with a speed of 400 km/h

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第7期25卷 525-540页

作者： Nianxun li Tian li Zhiyuan DAI Deng qin Jiye ZHANG State Key Laboratory of Rail Transit Vehicle System Southwest Jiaotong UniversityChengdu 610031China

The streamlined nose length(SNL)plays a crucial role in determining the aerodynamic performance of high-speed *** appropriate SNL can not only effectively reduce the magnitude of aerodynamic drag and lift forces,but also improve the performance of the high-speed train in tunnel passing and crosswind *** this study,a numerical simulation of the aerodynamic performance of high-speed trains at a speed of 400 km/h,with varying SNLs,is conducted using the k-ωshear stress transport(SST)turbulence *** different SNLs include 6.0,7.0,8.0,9.0,9.8,12.0,15.0,and 18.0 *** order to validate the accuracy of the numerical simulation,its results are compared with wind tunnel test data obtained from the *** simulation is carried out using compressible and incompressible gases to determine the effect of gas compressibility on *** impact of SNL on the aerodynamic performance of the trains is analyzed in terms of aerodynamic forces,velocity,and pressure *** comparison to the original train,the train with a 6.0 m SNL experienced a 10.8%increase in overall aerodynamic ***,the lift forces on the head and tail cars increased by 35.7%and 75.5%,*** the other hand,the train with an 18.0 m SNL exhibited a 16.5%decrease in aerodynamic ***,the lift forces on the head and tail cars decreased by 21.9%and 49.7%,*** aerodynamic drag force of the entire train varies linearly with the SNL,while the aerodynamic lift of the tail car follows a quadratic function in relation to the SNL.

关键词： Streamlined nose length(SNL) High-speed train Aerodynamic performance Numerical simulation Flow structures

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Method for the classification of tea diseases via weighted sampling and hierarchical classification learning

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International Journal of Agricultural and Biological Engineering 2024年第3期17卷 211-221页

作者： Rujia li Weibo qin Yiting He Yadong li Rongbiao Ji Yehui Wu Jiaojiao Chen Jianping yang College of Big Data Yunnan Agricultural UniversityKunming 650201China College of Plant Protection Jilin Agricultural UniversityChangchun 130118China

This study proposed a weighted sampling hierarchical classification learning method based on an efficient backbone network model to address the problems of high costs,low accuracy,and time-consuming traditional tea disease recognition *** method enhances the feature extraction ability by conducting hierarchical classification learning based on the EfficientNet model,effectively alleviating the impact of high similarity between tea diseases on the model’s classification *** better solve the problem of few and unevenly distributed tea disease samples,this study introduced a weighted sampling scheme to optimize data processing,which not only alleviates the overfitting effect caused by too few sample data but also balances the probability of extracting imbalanced classification *** experimental results show that the proposed method was significant in identifying both healthy tea leaves and four common leaf diseases of tea(tea algal spot disease,tea white spot disease,tea anthracnose disease,and tea leaf blight disease).After applying the“weighted sampling hierarchical classification learning method”to train 7 different efficient backbone networks,most of their accuracies have *** EfficientNet-B1 model proposed in this study achieved an accuracy rate of 99.21%after adopting this learning method,which is higher than EfficientNet-b2(98.82%)and MobileNet-V3(98.43%).In addition,to better apply the results of identifying tea diseases,this study developed a mini-program that operates on *** can quickly obtain accurate identification results and corresponding disease descriptions and prevention methods through simple *** intelligent tool for identifying tea diseases can serve as an auxiliary tool for farmers,consumers,and related scientific researchers and has certain practical value.

关键词： tea diseases hierarchical classification learning weighted sampling classification method EfficientNet miniprogram

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Engineered spidroin-derived high-performance fibers for diverse applications

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Nano Research 2024年第2期17卷 492-502页

作者： Dawen qin Jingjing li Huanrong li Hongjie Zhang Kai liu National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization Tianjin Key Laboratory of Chemical Process SafetySchool of Chemical Engineering and TechnologyHebei University of TechnologyTianjin 300130China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Engineering Research Center of Advanced Rare Earth Materials Ministry of EducationDepartment of ChemistryTsinghua UniversityBeijing 100084China University of Science and Technology of China Hefei 230026China

Spider silks are well known for their exceptional mechanical properties that are tougher than Kevlar and ***,the restricted production amounts from their native sources limit applications of spider *** the decades,there have been significant interests in fabricating man-made silk fibers with comparable performance to natural silks,inspiring many efforts both for biosynthesizing recombinant spider silk proteins(spidroins)in amenable heterologous hosts and biomimetic spinning of artificial spider *** strategies provide promising routes to produce high-performance and functionally optimized fibers with diverse ***,we summarize the hosts that have been applied to produce recombinant *** addition,the fabrication and mechanical properties of recombinant spidroin fibers and their composite fibers are also ***,we demonstrate the applications of recombinant spidroin-based ***,facing the challenges in biosynthesis,scalable production,and hierarchical assembly of high-performance recombinant spidroins,we give a summary and perspective on future development.

关键词： spider silk recombinant spidroin heterologous expression fiber fabrication mechanical property

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OsFK1 encodes C-14 sterol reductase,which is involved in sterol biosynthesis and affects premature aging of leaves in rice

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The Crop Journal 2024年第4期12卷 1010-1021页

作者： Ping li Xia Zhang Wuzhong Yin yang Shui Jie Zhang Nana Xu Dasong Bai qingxiong Huang Yuanyuan li Pan Qi Xing li Qianlong li Shihong yang Guotao yang Hong Chen Peng qin Yungao Hu Youlin Peng Rice Research Institute School of Life Science and EngineeringSouthwest University of Science and TechnologyMianyang 621010SichuanChina Hainan Yazhou Bay Seed Laboratory Sanya 572025HainanChina Rice Research Institute State Key Laboratory of Crop Gene Exploration and Utilization in Southwest ChinaSichuan Agricultural UniversityChengdu 610061SichuanChina Yazhouwan National Laboratory Sanya 572704HainanChina

The enzyme C-14 sterol reductase is involved in biosynthesis of brassinosteroids(BR)and sterols,as well as plant ***1,a member of the sterol biosynthesis pathway located in the endoplasmic reticulum(ER),encodes C-14 sterol ***,there is little research on the function of C-14 sterol reductase in *** with the wild type,an osfk1 mutant showed dwarf phenotype and premature aging in the second leaf during the trefoil stage,and abnormal development of leaf veins during the tillering *** osfk1 mutant showed signs of aberrant PCD,as evidenced by TUNEL *** suggested that high ROS buildup caused DNA damage and ROS-mediated cell death in the *** osfk1 mutant also showed decreased chlorophyll content and aberrant chloroplast *** of the osfk1 mutant allele revealed a non-synonymous G to A mutation in the final intron,leading to early ***,we identified the OsFK1 allele,cloned it by Mutmap sequencing,and verified it by ***-MS/MS assays demonstrated that the osfk1 mutation caused lower phytosterol *** findings showed that the OsFK1 allele encoding C-14 sterol reductase is involved in phytosterol biosynthesis and mediates normal development of rice plants.

关键词： Leaf development ROS Phytosterol biosynthesis Rice

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SHARP POINTWISE-IN-TIME ERROR ESTIMATE OF L1 SCHEME FOR NONliNEAR SUBDIFFUSION EQUATIONS

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Journal of Computational Mathematics 2024年第3期42卷 662-678页

作者： Dongfang li Hongyu qin Jiwei Zhang School of Mathematics and Statistics Huazhong University of Science and TechnologyWuhan 430074China Hubei Key Laboratory of Engineering Modeling and Scientific Computing Huazhong University of Science and TechnologyWuhan 430074China School of Mathematics and Statistics Wuhan UniversityWuhan 430072China

An essential feature of the subdiffusion equations with theα-order time fractional derivative is the weak singularity at the initial *** weak regularity of the solution is usually characterized by a regularity parameterσ∈(0,1)∪(1,2).Under this general regularity assumption,we present a rigorous analysis for the truncation errors and develop a new tool to obtain the stability results,i.e.,a refined discrete fractional-type Grönwall inequality(DFGI).After that,we obtain the pointwise-in-time error estimate of the widely used L1 scheme for nonlinear subdiffusion *** present results fill the gap on some interesting convergence results of L1 scheme onσ∈(0,α)∪(α,1)∪(1,2].Numerical experiments are provided to demonstrate the effectiveness of our theoretical analysis.

关键词： Sharp pointwise-in-time error estimate Ll scheme Nonlinear subdiffusion equations Non-smooth solutions

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Current status and future development of aortic stent fenestration-assisted techniques

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Chinese Medical Journal 2024年第6期137卷 752-754页

作者： Gezheng Chen Xiaoyu Qi Wanying Wu Dongsheng Fu li qin Chao yang Department of Vascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Vascular Surgery Nanjing Drum Tower HospitalAffiliate Hospital of Nanjing University Medical SchoolNanjingJiangsu 210008China

To the Editor:In 1996,Parodi et al^([1])introduced the technique of fenestrated endovascular aneurysm repair(f-EVAR)for treatment of pararenal abdominal aortic *** use physician modified stent grafts(PMSGs)for fenestration,which allows for stent grafts to gain sufficient proximal sealing zones and maintain the blood perfusion of visceral arteries.^([2])Stent grafts used in the f-EVAR technique developed from PMSGs to Company Modified Devices(CMDs)and off-the-shelf devices.^([3])To improve the precision of PMSGs and shorten the time of stent graft modification,various techniques that assist in the process of fenestration have been researched and developed,including 3-dimensional(3D)printing technique,the Mixed Reality(MR)technique,and the 3D parametric surface planar topological guide *** article describes the current status and future development of these techniques.

关键词： aortic perfusion arteries

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Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex

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Chinese Chemical Letters 2024年第5期35卷 330-337页

作者： yang qin Jiangtian li Xuehao Zhang Kaixuan Wan Heao Zhang Feiyang Huang limei Wang Hongxun Wang Longjie li Xianjin Xiao Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pancreatic Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Wuhan Polytechnic University Wuhan 430023China

Strand displacement reaction is a crucial component in the assembly of diverse DNA-based nanodevices,with the toehold-mediated strand displacement reaction representing the prevailing ***,the single-stranded Watson-Crick sticky region that serves as the trigger for strand displacement can also cause leakage reactions by introducing crosstalk in complex DNA ***,we proposed the toeless and reversible DNA strand displacement reaction based on the Hoogsteen-bond triplex,which is compatible with most of the existing DNA *** demonstrated that our proposed reaction can occur at pH 5 and can be reversed at pH *** also observed an approximately linear relationship between the degree of reaction and pH within the range of pH 5-6,providing the potential for precise regulation of the ***,by altering the sequence orientation,we have demonstrated that our proposed reaction can be initiated or regulated through the same toeless mechanism without the requirement for protonation in low pH *** on the proposed reaction principle,we further constructed a variety of DNA nanodevices,including two types of DNA logic gates that rely on pH 5/pH 9 changes for initiating and reversing:the AND gate and the OR *** also successfully constructed a DNA Walker based on our proposed reaction modes,which can move along a given track after the introduction of a programmable DNA sequence and complete a cycle after 4 *** findings suggest that this innovative approach will have broad utility in the development of DNA circuits,molecular sensors,and other complex biological systems.

关键词： DNA strand displacement Toeless Triplex DNA strands Reversible DNA circuit DNA walker

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