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Journal of Pharmaceutical Analysis 2010年第2期22卷 97-101页

作者： qin xiao1,2.hui chen2.jin-ming lin2.1. college of ocean,hebei agricultural university,qinhuangdao 066003 2. The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology,Department of Chemistry,Tsinghua university,Beijing 100084,China. college of ocean Hebei Agricultural University Qinhuangdao 066003 China Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Department of Chemistry Tsinghua University Beijing 100084 China

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase,equipped with a polyhistidine affinity tag,was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108,and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein,the ATP content of bacteria was 9.48×10-16mol/mL,and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together,our results provided a simple and efficacious method of the preparation of recombinant luciferase,which could be applied in the determination of bacteria via ATP bioluminescence.

关键词： ATP bioluminescence bacterial detection expression firefly luciferase purification

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蔗渣木质素液化影响因素及其产物表征

林产工业 2024年第5期61卷 6-11,18页

作者：覃琼林刘金明谢恩俊冯睿张建辉甘卫星广西师范大学环境与资源学院广西桂林541000 广西广林新材木业集团广西南宁530022 国家林业和草原局产业发展规划院北京100010

生物质液化是实现农林废弃物的高值转化和梯次化替代化工原料的重要技术手段。本文选用有机溶剂液化法对蔗渣木质素进行液化,探究不同液化参数(催化剂量,液固比,液化温度,液化时间)对其液化效率的影响,并结合正交试验进行液化工艺优化,... 详细信息

生物质液化是实现农林废弃物的高值转化和梯次化替代化工原料的重要技术手段。本文选用有机溶剂液化法对蔗渣木质素进行液化,探究不同液化参数(催化剂量,液固比,液化温度,液化时间)对其液化效率的影响,并结合正交试验进行液化工艺优化,同时对液化产物进行表征。结果表明:蔗渣木质素液化优化工艺参数为:液化温度150℃,液化时间1 h,液固比3∶1,催化剂量15%,此时残渣率为0.87%。蔗渣木质素在苯酚和硫酸的共同作用下,由原本的球形、球凹形结构降解成残渣颗粒。液化反应通过裂解、脱水、脱氢、脱氧和脱羧等方式,断裂连接蔗渣木质素大分子结构间的醚键和碳碳键,并通过缩合、羟甲基化和醚化反应重新排列液化中间产物中的小分子片段,最终形成较稳定的、可溶于苯酚的复杂混合物。

关键词：木质素蔗渣苯酚液化液化产物

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外源褪黑素对桃生长及果实品质的影响

果树学报 2021年第1期38卷 40-49页

作者：吴彩芳李红艳刘琴廖明安刘磊吕秀兰梁东王进夏惠林立金陈栋涂美艳四川农业大学果蔬研究所成都611130 重庆市林业科学研究院重庆400036 四川农业大学园艺学院成都611130 四川省农业科学院园艺研究所成都610066

【目的】探讨外源褪黑素对桃生长和果实品质的影响。【方法】以早熟桃‘早蜜’为试材,于果实膨大期对其叶片喷施不同浓度的褪黑素,测定了桃果实成熟期的新梢生长量、生理状况和果实品质指标。【结果】50~150μmol·L^-1的褪黑素处理能... 详细信息

【目的】探讨外源褪黑素对桃生长和果实品质的影响。【方法】以早熟桃‘早蜜’为试材,于果实膨大期对其叶片喷施不同浓度的褪黑素,测定了桃果实成熟期的新梢生长量、生理状况和果实品质指标。【结果】50~150μmol·L^-1的褪黑素处理能促进桃新梢的生长,增加其茎长和茎粗。150μmol·L^-1褪黑素提高了桃叶片超氧化物歧化酶活性,但其余处理较对照有所降低;50~100μmol·L^-1褪黑素提高了桃叶片过氧化氢酶活性,其余处理较对照有所降低。褪黑素提高了桃果实的苯丙氨酸解氨酶、抗坏血酸过氧化酶和脂氧合酶活性,降低了多酚氧化酶活性。100~150μmol·L^-1褪黑素增加了桃果实单果质量、果实纵横径,降低了果实硬度,提高了果实维生素C、可溶性固形物、蔗糖、果糖和山梨醇含量,降低了葡萄糖含量。【结论】褪黑素能够促进桃生长、改善桃果实品质。

关键词：桃褪黑素生长果实品质

372份花生种质资源的耐冷性评价及耐冷种质筛选

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中国油料作物学报 2023年第6期45卷 1247-1257页

作者：张玉松符明联雷永罗怀勇张丽芹陆建美舒兴香普兴林何柳罗金超陈晓艳刘珏王绍彩原小燕姜慧芳云南省农业科学院经济作物研究所云南昆明650205 中国农业科学院油料作物研究所湖北武汉430062 东川区经济作物技术推广站云南东川654100 云南省砚山县农业技术中心云南砚山663100 云南大学农学院云南昆明650500 大理州农业科学研究院云南大理671600 德宏州农业技术推广中心云南芒市678400 盐津县农业技术推广中心云南盐津657500

低温是限制花生生长的重要非生物胁迫因子之一,选育耐冷品种是解决低温冷害最直接有效的手段。本研究以国内外收集的372份花生种质资源为研究对象,对其在温暖区(武汉)和冷凉区(昆明)两种不同生态环境下的产量相关性状及品质进行分析,采... 详细信息

低温是限制花生生长的重要非生物胁迫因子之一,选育耐冷品种是解决低温冷害最直接有效的手段。本研究以国内外收集的372份花生种质资源为研究对象,对其在温暖区(武汉)和冷凉区(昆明)两种不同生态环境下的产量相关性状及品质进行分析,采用隶属函数法结合连锁聚类分析,筛选优异耐冷资源,并分析冷凉气候对产量相关因子及品质的影响,筛选关键耐冷性鉴定指标,对其耐冷性进行鉴定评价。结果表明,花生的植株、产量和品质主要受结荚期和饱果成熟期温度、光照和积温等影响,温度和积温越高、光照时间越长,产量和含油量、蛋白质等含量越高,油酸、亚油酸和蔗糖含量越低,且不同材料对低温的耐受性存在差异。对综合耐冷评价值(D)进行聚类分析,将372份材料划分为4类,第Ⅰ类属于强耐冷型共2份;第Ⅱ类属于耐冷型共86份材料;第Ⅲ类属于不耐冷型共280份材料;第Ⅳ类属于敏感型共4份材料。采用多元逐步回归分析法,建立花生田间耐冷性评价模型Y=-0.002+0.003X1+0.008X2+0.002X3+0.001X4,并筛选出相对百仁重、相对单株果数、相对株高和相对单株产量可作为花生田间耐冷性初筛选指标。本研究为花生耐冷性新品种培育和相关理论研究提供基础材料和高效鉴定方法。

关键词：花生耐冷性评价指标隶属函数法多元回归分析法

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山黧豆根腐病病原菌亚洲镰孢菌的分离鉴定

草地学报 2021年第6期29卷 1350-1356页

作者：邢会琴王春明金社林周天旺郭成河西学院农业与生态工程学院甘肃张掖734000 甘肃省农业科学院植物保护研究所甘肃兰州730070

为了明确山黧豆(Lathyrus sativus)根腐病病原菌的分类地位,本研究于2.18年8月在四川省南充市采集山黧豆根腐病病样,经组织分离法进行分离纯化,采用形态学和翻译延伸因子(Translation elongation factor 1-agene,EF-1α)基因序列分析相... 详细信息

为了明确山黧豆(Lathyrus sativus)根腐病病原菌的分类地位,本研究于2.18年8月在四川省南充市采集山黧豆根腐病病样,经组织分离法进行分离纯化,采用形态学和翻译延伸因子(Translation elongation factor 1-agene,EF-1α)基因序列分析相结合的方法对致病菌进行鉴定。结果表明:从采集的山黧豆根腐病病样上共分离获得39株分离物,其中3株分离物Shan-27,Shan-28和Shan-29对山黧豆具有强致病性,分离频率为7.69%;这3株致病菌在形态学上均接近于禾谷镰孢菌复合种(Fusarium graminearum species complex),而对其翻译延伸因子(EF-1α)序列分析发现,其均与亚洲镰孢菌聚在同一分支上,同源性达99%以上。因此,根据菌株形态学特征和EF-1α基因序列分析结果,将引致山黧豆根腐病的病原菌确定为亚洲镰孢菌(Fusarium asiaticum)。

关键词：山黧豆根腐病亚洲镰孢菌病原鉴定 EF-1α

Fungal diversity notes 111-252-taxonomic and phylogenetic contributions to fungal taxa

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Fungal Diversity 2015年第6期75卷 27-274页

作者： Hiran A.Ariyawansa Kevin D.Hyde Subashini C.Jayasiri Bart Buyck K.W.Thilini Chethana Dong qin Dai Yu cheng Dai Dinushani A.Daranagama Ruvishika S.Jayawardena Robert Lücking Masoomeh Ghobad-Nejhad Tuula Niskanen Kasun M.Thambugala Kerstin Voigt Rui lin Zhao Guo-Jie Li mingkwan Doilom Saranyaphat Boonmee Zhu L.Yang qing Cai Yang-Yang Cui Ali H.Bahkali Jie chen Bao Kai Cui Jia Jia chen Monika C.Dayarathne Asha J.Dissanayake Anusha H.Ekanayaka Akira Hashimoto Sinang Hongsanan E.B.Gareth Jones Ellen Larsson Wen jing Li Qi-Rui Li Jian Kui Liu Zong Long Luo Sajeewa S.N.Maharachchikumbura Ausana Mapook Eric H.C.McKenzie Chada Norphanphoun Sirinapa Konta Ka Lai Pang Rekhani H.Perera Rungtiwa Phookamsak Chayanard Phukhamsakda Umpava Pinruan Emile Randrianjohany Chonticha Singtripop Kazuaki Tanaka cheng ming Tian Saowaluck Tibpromma Mohamed A.Abdel-Wahab Dhanushka N.Wanasinghe Nalin N.Wijayawardene jin-Feng Zhang Huang Zhang Faten A.Abdel-Aziz Mats Wedin Martin Westberg Joseph F.Ammirati Timur S.Bulgakov Diogo X.Lima Tony M.Callaghan Philipp Callac cheng-Hao Chang Luis F.Coca Manuela Dal-Forno Veronika Dollhofer Kateřina Fliegerová Katrin Greiner Gareth W.Griffith Hsiao-Man Ho Valerie Hofstetter Rajesh Jeewon Ji Chuan Kang Ting-Chi Wen Paul M.Kirk Ilkka Kytövuori James D.Lawrey Jia Xing Hong Li Zou Yi Liu Xing Zhong Liu Kare Liimatainen H.Thorsten Lumbsch Misato Matsumura Bibiana Moncada Salilaporn Nuankaew Sittiporn Parnmen AndréL.C.M.de Azevedo Santiago Sujinda Sommai Yu Song Carlos A.F.de Souza Cristina M.de Souza-Motta Hong Yan Su Satinee Suetrong Yong Wang Syuan-Fong Wei Ting Chi Wen Hai Sheng Yuan Li Wei Zhou Martina Réblová Jacques Fournier Erio Camporesi J.Jennifer Luangsa-ard Kanoksri Tasanathai Artit Khonsanit Donnaya Thanakitpipattana Sayanh Somrithipol Paul Diederich Ana M.Millanes Ralph S.Common Marc Stadler Ji Ye Yan XingHong Li Hye Won Lee Thi T.T.Nguyen Hyang Burm Lee Eliseo Battistin Orlando Marsico Alfredo Vizzini Jordi Vila Enrico Ercole Ursula Eberhardt Giampaolo Simonini Hua-An Wen Xin-Hua chen Otto Mie Guizhou Key Laboratory of agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouChina Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanChina Centre of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailan Département Systématique et Evolution CP 39ISYEBUMR 7205 CNRS MNHN UPMC EPHEMuséum National d’Histoire Naturelle12 Rue BuffonF-75005 ParisFrance Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesNo.9 of Shu Guang Hua Yuan Zhong LuHaidian DistrictBeijing 10097China Microbial Drugs Helmholtz-Centre for Infection Research Inhoffenstrasse 738124 BraunschweigGermany Staatliches Museum für Naturkunde Stuttgart Rosenstein 1D-70191StuttgartGermany Institute of Microbiology Beijing Forestry UniversityPO Box 61Beijing 100083China State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesNo 31st West Beichen RoadChaoyang DistrictBeijing 100101China The Herbarium(ANDA) Universitas AndalasPadangSumatera Barat 25163Indonesia Botanischer Garten&Botanisches Museum Berlin Freie Universität BerlinKönigin-Luise-Straße 6-814195 BerlinGermany Department of Biotechnology Iranian Research Organization for Science and Technology(IROST)P.O.Box 3353-5111Tehran ***Iran Jodrell Laboratory Royal Botanic GardensKew TW93DSUK Leibniz Institute for Natural Product Research and Infection Biology Jena Microbial Resource CollectionAdolf-Reichwein-Strasse 2307745 JenaGermany Department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box:2455Riyadh 1145Saudi Arabia The United Graduate School of agricultural Sciences Iwate University18-8 Ueda 3 ChomeMorioka 020-8550Japan Faculty of Agriculture and Life Sciences Hirosaki University3 Bunkyo-choHirosakiAomori 036-8561Japan Department of Biological and Environmental Sciences University of GothenburgBox 461SE

This paper is a compilation of notes on 142 fungal taxa,including five new families,20 new genera,and 100 new species,representing a wide taxonomic and geographic *** new families,Ascocylindricaceae,Caryosporaceae and Wicklowiaceae(Ascomycota)are introduced based on their distinct lineages and unique *** new Dothideomycete genera Pseudomassariosphaeria(Amniculicolaceae),Heracleicola,Neodidymella and Pseudomicrosphaeriopsis(Didymellaceae),Pseudopithomyces(Didymosphaeriaceae),Brunneoclavispora,Neolophiostoma and Sulcosporium(Halotthiaceae),Lophiohelichrysum(Lophiostomataceae),Galliicola,Populocrescentia and Vagicola(Phaeosphaeriaceae),Ascocylindrica(Ascocylindricaceae),Elongatopedicellata(Roussoellaceae),Pseudoasteromassaria(Latoruaceae)and Pseudomonodictys(Macrodiplodiopsidaceae)are *** newly described species of Dothideomycetes(Ascomycota)are Pseudomassariosphaeria bromicola(Amniculicolaceae),Flammeascoma lignicola(Anteagloniaceae),Ascocylindrica marina(Ascocylindricaceae),Lembosia xyliae(Asterinaceae),Diplodia crataegicola and Diplodia galiicola(Botryosphaeriaceae),Caryospora aquatica(Caryosporaceae),Heracleicola premilcurensis and Neodidymella thailandicum(Didymellaceae),Pseudopithomyces palmicola(Didymosphaeriaceae),Floricola viticola(Floricolaceae),Brunneoclavispora bambusae,Neolophiostoma pigmentatum and Sulcosporium thailandica(Halotthiaceae),Pseudoasteromassaria fagi(Latoruaceae),Keissleriella dactylidicola(Lentitheciaceae),Lophiohelichrysum helichrysi(Lophiostomataceae),Aquasubmersa japonica(Lophiotremataceae),Pseudomonodictys tectonae(Macrodiplodiopsidaceae),Microthyrium buxicola and Tumidispora shoreae(Microthyriaceae),Alloleptosphaeria clematidis,Allophaeosphaeria cytisi,Allophaeosphaeria subcylindrospora,Dematiopleospora luzulae,Entodesmium artemisiae,Galiicola pseudophaeosphaeria,Loratospora luzulae,Nodulosphaeria senecionis,Ophiosphaerella aquaticus,Populocrescentia forlicesenensis and Vagicola vagans(Phaeosphaeriaceae),Elon

关键词： Fungi Taxonomy New genus New species Phylogeny