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Finite Element Simulation of Cold-Rolling Process of Shaped Steel Tube for Driving Shaft

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Journal of Iron and Steel Research International 2010年第8期17卷 25-29页

作者： SHUAI Mei-rong LIU Shao-bo GAO Cong-min qin jian-ping College of Material and Science Engineering Taiyuan University of Science and TechnologyTaiyuan 030024ShanxiChina Xi'an Heavy Machinery Research Institute Xi'an 710032ShaanxiChina

Based on the deformation characteristics of Y-type mill,a finite element model for simulating the formation process of shaped steel tube for driving shaft is *** distributions of stress and strain were obtained from the *** outer diameter and transverse wall thickness were also analyzed *** was done on Y-type mill.A comparison between simulation results and experiment results shows that the simulated results of shaped steel tube are in good agreement with the on-site *** model could provide the basis for theoretical research and engineering applications of shaped steel tube rolling process.

关键词： shaped steel tube Y-type mill cold-rolling simulation experiment

Transconjugation and genotyping of the plasmid-mediated AmpC β-lactamase and extended-spectrum β-lactamase genes in Klebsiella pneumoniae

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Chinese Medical Journal 2009年第9期122卷 1092-1096页

作者： SHI Wei-feng ZHOU Jun qin jian-ping Department of Clinical Laboratory Third Affiliated Hospital ofSuzhou University Changzhou Jiangsu 213003 China Department of Respiratory Medicine Third Affiliated Hospital ofSuzhou University Changzhou Jiangsu 213003 China Department of Clinical Laboratory Changzhou Traditional ChineseMedicine Hospital Changzhou Jiangsu 213003 China

Bsckgroud AmpC β-lactamases and extended-spectrum β-lactamases （ESBLs） are becoming predominant causes of resistance to third and forth-generation cephalosporins in Klebsiella pneumoniae （K. pneumoniae）. It is very difficult to treat infectious diseases caused by multidrug-resistant K. pneumoniae. The purpose of the present study was to investigate transconjugation and characteristics of β-lactamase genes in K. pneumoniae producing AmpC β-lactamases and ESBLs. Methods AmpC β-lactamases were detected by three-dimension test and ESBLs by disc confirmatory test. Minimum inhibitory concentrations （MICs） were determined by agar dilution. Transfer of resistance to EC600 （Rifr） was attempted by conjugation in broth and screened on agar containing cefotaxime （2 μg/ml） plus rifampin （1024 μg/ml）. The genes encoding AmpC or ESBLs and their transconjugants were detected by PCR and verified by DNA sequencing. Results The resistant rates to ampicillin and piperacillin were 100% in 18 isolates of K. pneumoniae. However, imipenem was still of great bactericidal activity on K. pneumoniae, and its MIC50 was 0.5 μg/mL. Eleven β-lactamase genes, including TEM-1, TEM-11, SHV-13, SHV-28, CTX-M-9, CTX-M-22, CTX-M-55, OXA-1, LEN, OKP-6 and DHA-1, were found from 18 isolates. And at least one β-lactamase gene occurred in each isolate. To our surprise, there were six β-lactamase genes in the CZ04 strain. Among 18 isolates of K. pneumoniae, the partial resistant genes in 8 isolates were conjugated successfully, which had 100% homological sequence with donors by sequence analysis. Compared with donors, 8 transconjugants had attained resistance to most β-lactams, including ampicillin, piperacillin, cefoxitin, cefotaxime and aztreonam, or even amikacin and gentamicin. Conclusions R plasmids can be easily transferred between the resistant and sensitive negative bacilli. It is very difficult to block and prevent the spread of antimicrobial resistance. So more attention should be paid to red

关键词： Klebsiella pneumoniae plasmid transconjugation β-lactamase

An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis

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Hepatobiliary & Pancreatic Diseases International 2008年第1期7卷 51-57页

作者： jiang, Ming-De Zheng, Shu-Mei Xu, Hui Zeng, Wei-Zheng Zhang, Yong Sun, Hao-ping Wang, Yun-Xia qin, jian-ping Wu, Xiao-Ling Gen Hosp Chenghu Milt Command Dept Gastroenterol Chengdu 610083 Peoples R China

BACKGROUND: The pathogenesis of hepatic fibrosis and cirrhosis is still not fully understood. The extracellular signal-regulated kinase (ERK) pathway is involved in the regulation of cell proliferation and differentiation. The aim of this study was to investigate the effects of PD98059, a specific inhibitor of ERK, on the cell cycle, cell proliferation, secretion of type I collagen and expression of cyclin D1 mRNA, CDK4 mRNA and transforming growth factor-beta 1 (TGF-beta 1) mRNA in rat hepatic stellate cells (HSCs) stimulated by acetaldehyde. METHODS: Rat HSCs stimulated by acetaldehyde were incubated with PD98059 at different concentrations. The cell cycle was analysed by flow cytometry. Cell proliferation was assessed by the methyl thiazolyl tetrazolium colorimetric assay. The mRNA expression of cyclin D1, CDK4 and TGF-beta 1 was examined using the reverse transcriptase-polymerase chain reaction. Type I collagen in the culture medium was detected by enzyme-linked immunosorbent assay. RESULTS: 20, 50 and 100 mu mol/L PD98059 significantly inhibited the proliferation and provoked a G0/G1-phase arrest of acetaldehyde-induced HSCs in a dose-dependent manner. The secretion of type I collagen and the expression of cyclin D1, CDK4 and TGF-beta 1 mRNA in acetaldehyde-induced HSCs were markedly inhibited by 50 and 100 mu mol/L PD98059, respectively. CONCLUSIONS: The ERK pathway regulates the cell proliferation, secretion of type I collagen and the expression of TGF-beta 1 mRNA in rat HSCs stimulated by acetaldehyde, which is likely related to its regulative effect on the cell cycle.

关键词： extracellular signal-regulated kinase hepatic stellate cell hepatic fibrosis

Silencing transformer and transformer-2 in Zeugodacus cucurbitae causes defective sex determination with inviability of most pseudomales

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Journal of Integrative Agriculture 2024年第3期23卷 938-947页

作者： qin Ma Zizhen Fan ping Wang Siya Ma jian Wen Fengqin Cao Xianwu Lin Rihui Yan Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests of Ministry of Education School of Plant ProtectionHainan UniversityHaikou 570228China School of Life Sciences HainanUniversityHaikou 570228China Hainan Yazhou Bay Seed Lab Sanya 572000China

transformer is a switch gene for sex determination in many insects, which cooperates with transformer-2 that is expressed in both sexes to regulate female differentiation, particularly in dipterans. Zeugodacus cucurbitae(Coquillett) is a very destructive pest worldwide, however, its sex determination pathway remains largely uncharacterized. Here, we show that the female sex ratio is sharply reduced with knockdown of either transformer or transformer-2 by RNA interference in early embryos of Z. cucurbitae. Most of the males grown from the embryos with transient transformer and transformer-2 suppression mated with wild-type females and produced mixed sex progeny, with one exception that produced only female progeny, and all of the few remaining males failed to mate with wild-type females and produced no progeny. The exceptional male and those males with mating failure were XX pseudomales as determined by the detection of Y chromosome-linked Maleness-on-the-Y, indicating that most XX pseudomales are not viable. The phenotypes of transformer and transformer-2 suggest that they play a key role in regulating sex determination and are required for female sexual development of Z. cucurbitae. Our results will be beneficial to the understanding of sex determination in Z. cucurbitae and can facilitate the development of genetic sexing strains for its biological control.

关键词： Zeugodacus cucurbitae transformer transformer-2 sex determination RNA interference biological control

On entire solutions of some Fermat type differential-difference equations

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Applied Mathematics(A Journal of Chinese Universities) 2024年第1期39卷 69-88页

作者： LONG jian-ren qin Da-zhuan School of Mathematical Sciences Guizhou Normal UniversityGuiyang 550025China

On one hand,we study the existence of transcendental entire solutions with finite order of the Fermat type difference *** the other hand,we also investigate the existence and growth of solutions of nonlinear different... 详细信息

关键词： entire solutions differential-difference equations existence finite order

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桥头台背路堤与桥台设计方案评价

长安大学学报（自然科学版） 2008年第4期28卷 47-50页

作者：秦建平长安大学特殊地区公路工程教育部重点实验室陕西西安710064

为了研究桥头路堤不均匀沉降,分析了桥头台背地基、地面与路堤的变形所产生的影响,研究了轻型桥台耳墙、牛腿、伸缩缝、桥头塔板的结构设计功能,并对设计方案进行了改进和完善。结果表明:在台背10 m范围内对地基进行处治,可明显减少路... 详细信息

为了研究桥头路堤不均匀沉降,分析了桥头台背地基、地面与路堤的变形所产生的影响,研究了轻型桥台耳墙、牛腿、伸缩缝、桥头塔板的结构设计功能,并对设计方案进行了改进和完善。结果表明:在台背10 m范围内对地基进行处治,可明显减少路堤工后沉降;取消耳墙、牛腿的桥台结构方案,可以扩大台背压实工作面,有效提高压实度;建立的搭板变位模型可预测台背路堤工后沉降引起的搭板刚性支承端变位量。

关键词：桥梁工程桥头桥台设计方案

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路面水流线模型及水文计算参数选择

长安大学学报（自然科学版） 2007年第5期27卷 53-57页

作者：秦建平长安大学特殊地区公路工程教育部重点实验室陕西西安710064

根据几何学中空间平面对水平投影面最大坡度线原理以及解析几何中二次抛物线在凸凹型竖曲线的最高、最低点几何构成方法,应用软件CAD绘图功能进行二次开发,分析了高速公路曲线路段的路面排水规律,建立了公路路面水流线合成坡度大小和水... 详细信息

根据几何学中空间平面对水平投影面最大坡度线原理以及解析几何中二次抛物线在凸凹型竖曲线的最高、最低点几何构成方法,应用软件CAD绘图功能进行二次开发,分析了高速公路曲线路段的路面排水规律,建立了公路路面水流线合成坡度大小和水流线方向的模型。该模型可以定量计算路面汇水区大小,并确定排水设施的设置。同时针对公路几何线形划分,提出不同线形组合排水设计的参数选择模式,并对《公路排水设计规范》(JTJ18-97)中水力计算公式的参数选择提出了修正。

关键词：道路工程路面排水合成坡度最大坡度线参数

Reproductive biology of Hemitripterus villosus in the Bohai Strait,China

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Journal of Oceanology and Limnology 2024年第3期42卷 881-892页

作者： ping SUN Song qin Zhengyi LIU Longchuan ZHUANG Key Laboratory of Coastal Biology and Biological Resource Utilization Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

To understand the reproductive biology of Hemitripterus villosus in the Bohai Strait,and fill the key knowledge gap in verifying reproductive seasonality and documenting gonadotropin dependent reproductive cycle of this species,a year-round detail study was conducted for the first *** total,263 individuals were randomly sampled off the coasts of the Bohai *** show that and the overall female-to-male ratio(1.35:1)did not differ significantly from the 1:1 sex *** length and weight relationship for females and males were regressed,depicting an isometric growth for both *** reproductive phases of the complete gonadotropin dependent reproductive cycle in both sexes were *** monthly variations in the gonadosomatic and hepatosomatic indexes for both sexes further indicated that this species spawns a single batch of eggs per year and the peak spawning period is from October to *** average diameter of eggs ranged from 2.10 to 3.57 *** length at first sexual maturity was 224.87 mm for females and 241.26 mm for *** positive relationships between absolute fecundity and fish size,and also gonadal weight were *** from the Bohai Strait exhibited smaller body size,smaller egg diameter,lower gonadosomatic index,lower absolute fecundity,and higher population extinction risk than the other populations’studied *** findings contribute to understanding the status of this isolated westernmost population,and we emphasize that more efficient protection measures for the species and control measures are urgently required to improve the coastal environment in the Bohai Strait.

关键词： Hemitripterus villosus Bohai Strait fecundity length at first maturity reproductive seasonality

Chain Extender-induced Hydrogen Bonding Organization Determines the Morphology and Properties of Thermoplastic Polycarbonate Polyurethane

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Chinese Journal of Polymer Science 2024年第1期42卷 87-96,I0009页

作者： Yi-Lang qin ping Zhu Chen-Xi Ouyang Xia Dong Fuwai Hospital Chinese Academyof Medical Sciences&Peking Union Medical College/National Center for Cardiovascular DiseasesBejing 100037China CASKey Laboratoryof Engineering Plastics CAS Research/Education Centerfor Excellence in Molecular SciencesInstitute ofChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Thermoplastic polycarbonate polyurethanes(PCUs) are multiblock copolymers that have been applied for medical devices for long time. Aliphatic diols are common chain extenders(CE) involved in the composition of the hard segments of PCU. However, limited knowledge was discovered about how the chemical structure of CE affects the hydrogen bonding organization within PCUs and their mechanical *** investigate this problem, a group of PCUs were synthesized respectively by extending the polymer chain with 1,4-butanediol(BDO),aminoethanol(MEA), ethanediol(EO) as three kinds of chain extenders. Tiny differences in the CE chemical structure results in remarkable variations in phase separation, condensed morphologies, thermal and mechanical properties, which are characterized by Fourier transform infrared spectrometer, atomic force microscopy, small-angle X-ray scattering, differential scanning calorimetry, and tensile tests. The microstructural evolution during unilateral deformation and the different mechanism induced by the different CEs was probed and unveil by in situ wide-and small-angle X-ray diffraction. Symmetry of CE can improve the organization of the hydrogen bonding. The coherence strength of the urethane/urea group also plays a key role by comparing the two PCUs with ethanediol and aminoethanol.

关键词： Thermoplastic polycarbonate polyurethane Chain extender Hydrogen bonding Mechanical properties