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E3 ubiquitin ligase PbrATL18 is a positive factor in pear resistance to drought and Colletotrichum fructicola infection

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Horticultural Plant Journal 2024年第3期10卷 698-712页

作者： Likun Lin qiming chen Kaili Yuan Caihua Xing Qinghai Qiao Xiaosan Huang Shaoling Zhang State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture Nanjing Agricultural UniversityNanjingJiangsu210095China

The Arabidopsis Toxicos en Levadura(ATL)protein is a subfamily of the E3 ubiquitin ligases,which exists widely in plants and is extensively involved in plant growth and development.Although the ATL family has been identified in other species,such as Arabidopsis,Oryza sativa,and grapevine,few reports on pear ATL gene families have been reported.In this study,92 PbrATL genes were identified and analyzed from the Pyrus breschneideri genome.Motif analysis and phylogenetic tree generation divided them into nine subgroups,and chromosome localization analysis showed that the 92 PbrATL genes were distributed in 16 of 17 pear chromosomes.Transcriptome data and quantitative real-time polymerase chain reaction(qRT-PCR)experiments demonstrated that PbrATL18,PbrATL41,and PbrATL88 were involved in both pear drought resistance and Colletotrichum fructicola infection.In addition,Arabidopsis thaliana overexpressing PbrATL18 showed greater resistance to drought stress than the wild type(WT),and PbrATL18-silenced pear seedlings showed greater sensitivity to drought and C.fructicola infection than the controls.PbrATL18 regulated plant resistance by regulating chitinase(CHI),phenylalanine ammonia-lyase(PAL),polyphenol oxidase(PPO),catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD)activities.This study provided a reference for further exploring the functions of the PbrATL gene in drought resistance and C.fructicola infection.

关键词： Pear Colletotrichum fructicola Arabidopsis Toxicos en Levadura(ATL) Drought stress

Sodium Nitrate Passivation as a Novel Insulation Technology for Soft Magnetic Composites

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Engineering 2023年第1期20卷 134-142页

作者： Mi Yan qiming chen Dong Liu chen Wu Jian Wang State Key Laboratory of Silicon Materials School of Materials Science and EngineeringZhejiang UniversityHangzhou 310027China School of Chemical Engineering Shandong University of TechnologyZibo 255049China National Institute for Materials Science Tsukuba 305-0047Japan

Sodium nitrate passivation has been developed as a new insulation technology for the production of FeSiAl soft magnetic composites (SMCs). In this work, the evolution of coating layers grown at different pH values is investigated involving analyses on their composition and microstructure. An insulation coating obtained using an acidic NaNO_(3) solution is found to contain Fe2O_(3), SiO_(2), Al2O_(3), and AlO(OH). The Fe2O_(3) transforms into Fe3O4 with weakened oxidizability of the NO_(3)– at an elevated pH, whereas an alkaline NaNO_(3) solution leads to the production of Al2O_(3), AlO(OH), and SiO_(2). Such growth is explained from both thermodynamic and kinetic perspectives and is correlated to the soft magnetic properties of the FeSiAl SMCs. Under tuned passivation conditions, optimal performance with an effective permeability of 97.2 and a core loss of 296.4 mW∙cm−3 is achieved at 50 kHz and 100 mT.

关键词： Soft magnetic composites Surface passivation Insulation technology Growth mechanism Magnetic performance

Synergistic effect of V_(2)O_(5)and Bi_(2)O_(3) on the grain boundary structure of high-frequency NiCuZn ferrite ceramics

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Journal of Advanced Ceramics 2022年第6期11卷 912-921页

作者： Xiuyuan FAN Guohua BAI Zhenhua ZHANG qiming chen Jiaying JINa Jiafeng XUa Xuefeng ZHANG Mi YAN School of Materials Science and Engineering State Key Laboratory of Silicon MaterialsKey Laboratory of Novel Materials for Information Technology of Zhejiang ProvinceZhejiang UniversityHangzhou 310027China Institute of Advanced Magnetic Materials College of Materials&Environmental EngineeringHangzhou Dianzi UniversityHangzhou 310012China

High-frequency soft magnetic ferrite ceramics are desired in miniaturized and efficient power electronics but remain extremely challenging to deploy on account of the power loss(Pcv)at megahertz frequencies.Here,we prepared NiCuZn ferrite with superior high-frequency properties by V_(2)O_(5)and Bi_(2)O_(3)synergistic doping,which proves to be a potent pathway to reduce Pcv of the ferrite at megahertz frequencies.The sample doped with 800 ppm V_(2)O_(5)and 800 ppm Bi_(2)O_(3)yielded the most optimized magnetic properties with a Pcv of 113 kW/m^(3)(10 MHz,5 mT,25℃),an initial permeability(μi)of 89,and a saturation induction(Bs)of 340 mT,which is at the forefront of the reported results.These outstanding properties are closely related to the notable grain boundary structure,which features a new type of nano-Bi_(2)Fe_(4)O_(9)phase around ferrite grains and a Ca/Si/V/O amorphous layer.Our results indicate great strides in correlating the grain boundary structure with multiple-ion doping and set the scene for the developing high-frequency soft magnet ferrites.

关键词： NiCuZn ferrite megahertz application grain boundary Bi_(2)Fe_(4)O_(9) ion doping

Genome-wide identiﬁcation of the mitogen-activated protein kinase kinases in pear and their functional analysis in response to black spot

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Horticultural Plant Journal 2023年第4期9卷 681-692页

作者： Zan Zhang qiming chen Luting Jia Ming Qian Qinghai Qiao Xiaosan Huang Shaoling Zhang College of Horticulture State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingJiangsu 210095China

The mitogen-activated protein kinase(MAPK)cascade is crucial to plant growth,development,and stress responses.MAPK kinases(MAPKK)play a vital role in linking upstream MAPKK kinases(MAPKKK)with the downstream MAPK.Black spot is one of the most serious fungal diseases of pear which is an important part of the fruit industry in China.The MAPKK genes have been identified in many plants,however,none has been reported in pear(Pyrus bretschneideri).In order to explore whether MAPK gene of pear is related to black spot disease,we designed this experiment.The present study investigated eight putative PbrMAPKK genes obtained from the Chinese white pear genome.The phylogenetic analysis revealed that PbrMAPKK genes were divided into A,B,C,and D groups.These PbrMAPKK genes are randomly distributed on 7 out of 17 chromosomes and mainly originated from the whole-genome duplication(WGD)event.The expression analysis of PbrMAPKK genes in seven pear tissues and the leaves of susceptible and resistant varieties after Alternaria alternata infection by quantitative real-time PCR(qRT-PCR)identified seven candidate genes associated with resistance.Furthermore,virus-induced gene silencing(VIGS)indicated that PbrMAPKK6 gene enhanced resistance to pear black spot disease in pear.

关键词： Pyrus bretschneideri Mitogen-activated protein kinase kinase(MAPKK) Gene family Disease resistance Pear black spot

PbrChiA:a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation

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Horticulture Research 2023年第10期10卷 185-201页

作者： qiming chen Huizhen Dong Qionghou Li Xun Sun Xin Qiao Hao Yin Zhihua Xie Kaijie Qi Xiaosan Huang Shaoling Zhang State Key Laboratory of Crop Genetics and Germplasm Enhancement Centre of Pear Engineering Technology ResearchNanjing Agricultural UniversityNanjing 210095China

Pear ring rot,caused by the pathogenic fungi Botryosphaeria dothidea,seriously affects pear production.While the infection-induced reactive oxygen species(ROS)burst of infected plants limits the proliferation of B.dothidea during the early infection stage,high ROS levels can also contribute to their growth during the later necrotrophic infection stage.Therefore,it is important to understand how plants balance ROS levels and resistance to pathogenic B.dothidea during the later stage.In this study,we identified PbrChiA,a glycosyl hydrolases 18(GH18)chitinase-encoding gene with high infection-induced expression,through a comparative transcriptome analysis.Artificial substitution,stable overexpression,and virus induced gene silencing(VIGS)experiments demonstrated that PbrChiA can positively regulate pear resistance as a secreted chitinase to break down B.dothidea mycelium in vitro and that overexpression of PbrChiA suppressed infection-induced ROS accumulation.Further analysis revealed that PbrChiA can bind to the ectodomain of PbrLYK1b2,and this interaction suppressed PbrLYK1b2-mediated chitin-induced ROS accumulation.Collectively,we propose that the combination of higher antifungal activity from abundant PbrChiA and lower ROS levels during later necrotrophic infection stage confer resistance of pear against B.dothidea.

关键词： infection ChiA resistance

基于ChatGPT+Prompt的专利技术功效实体自动生成研究

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数据分析与知识发现 2024年第4期8卷 14-25页

作者：白如江陈启明张玉洁杨超山东理工大学信息管理研究院淄博255000 浙江大学公共管理学院杭州310058

【目的】针对专利技术功效实体的自动识别问题,智能感知生成专利文献中的关键技术功效,辅助专利技术功效矩阵高质量构建。【方法】本文提出将ChatGPT应用于专利技术功效实体抽取任务的新思路,使用ChatGPT+Prompt的方法实现专利技术词、... 详细信息

【目的】针对专利技术功效实体的自动识别问题,智能感知生成专利文献中的关键技术功效,辅助专利技术功效矩阵高质量构建。【方法】本文提出将ChatGPT应用于专利技术功效实体抽取任务的新思路,使用ChatGPT+Prompt的方法实现专利技术词、功效词以及技术-功效二元组的识别、提取和生成。【结果】本文识别生成了4个领域、三种语言的专利技术功效实体,跨领域、跨语言、提示样本数量对比的实验结果(ROUGE值)表明,该方法能够较为准确地识别技术功效二元组。新能源汽车领域效果最佳,英文专利表现最优,跨域能力和跨语言能力显著,给予One-Shot显著提升模型性能。【局限】本文方法仍存在Prompt不标准、生成内容重复、单轮或多轮问答的选择困难等问题。【结论】本文方法具备合理性和可行性,有效降低技术功效实体生成的人力成本和任务门槛,拓展AIGC的应用场景,释放ChatGPT在专利文献挖掘的潜力。

关键词：专利技术功效矩阵技术词功效词实体识别生成式模型 ChatGPT Prompt

一种基于改进双二极管脉波倍增电路的串联型24脉波整流器

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中国电机工程学报 2024年第12期44卷 4878-4890,I0023页

作者：王景芳赵晨姚绪梁于天龙刘腾陈启明哈尔滨工程大学智能科学与工程学院黑龙江省哈尔滨市150001 哈尔滨工业大学电气工程及自动化学院黑龙江省哈尔滨市150006

为了在不增加串联型12脉波整流器中移相变压器复杂度的前提下,有效地抑制整流器的输入电流谐波,该文提出一种基于改进双二极管脉波倍增电路(improved dual-diode pulse-doubling circuit,IDPC)的串联型24脉波整流器。提出的IDPC被安装... 详细信息

为了在不增加串联型12脉波整流器中移相变压器复杂度的前提下,有效地抑制整流器的输入电流谐波,该文提出一种基于改进双二极管脉波倍增电路(improved dual-diode pulse-doubling circuit,IDPC)的串联型24脉波整流器。提出的IDPC被安装在整流器的直流侧并由单相自耦变压器、平衡电抗器、2个电容器和2个辅助二极管组成。IDPC中的辅助二极管根据整流器中2个三相整流桥的输出电压和单相自耦变压器绕组电压之间的关系交替导通,使IDPC周期性工作在3种模态下,然后根据交直流侧电压和电流关系将整流器的脉波数从12增加到24,使得输入电流的THD减小为原来的二分之一,有效地抑制输入电流谐波。因IDPC中磁性器件的总容量仅为输出功率的2.35%,辅助二极管的电流应力仅为输出电流的6.8%,提出的方案具有电路结构简单、容量小,成本低和导通损耗小的优点。与类似结构相比,提出的整流器在中高压工业场合具有更好的应用前景。搭建了一台输出功率为1 kW的实验样机,实验结果验证了理论分析的有效性和正确性。

关键词：串联24脉波整流器电流谐波脉波倍增双辅助二极管

Cronobacter spp.,foodborne pathogens threatening neonates and infants

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Frontiers of Agricultural Science and Engineering 2018年第3期5卷 330-339页

作者： qiming chen Yang ZHU Zhen QIN Yongjun QIU Liming ZHAO School of Biotechnology State Key Laboratory of Bioreactor EngineeringR&D Center of Separation and Extraction Technology in Fermentation IndustryEast China University of Science and TechnologyShanghai 200237China Shanghai Collaborative Innovation Center for Biomanufacturing Technology(SCICBT) Shanghai 200237China Bioprocess Engineering Group Agrotechnology and Food SciencesWageningen University and Research6700 AAWageningenthe Netherlands

Cronobacter spp.(formerly Enterobacter sakazakii) are special foodborne pathogens. Cronobacter infection can cause necrotizing enterocolitis, sepsis and meningitis in all age groups, especially neonates and infants, with a high fatality of up to 80%, although the infection is rare. Outbreaks of Cronobacter infection are epidemiologically proven to be associated with contaminated powdered infant formula(PIF). Cronobacter spp.can resist dry environments and survive for a long period in food with low water activity. Therefore, Cronobacter spp.have become serious pathogens of neonates and infants, as well as in the dairy industry. In this review, we present the taxonomy, pathogenesis, resistance, detection and control of Cronobacter spp.

关键词： Cronobacter spp. desiccation resistance pathogen control pathogen detection powdered infant formula

Effects of dietary arginine levels on growth performance,body composition, serum biochemical indices and resistance ability against ammonia-nitrogen stress in juvenile yellow catfish(Pelteobagrus fulvidraco)

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Animal Nutrition 2016年第3期2卷 204-210页

作者： qiming chen Hongxia Zhao Yanhua Huang Junming Cao Guoxia Wang Yuping Sun Yongjuan Li Institute of Animal Science Guangdong Academy of Agricultural Sciences College of Animal Science South China Agricultural University Guangdong Public Laboratory of Animal Breeding and Nutrition Guangdong Key Laboratory of Animal Breeding and Nutrition

This experiment was conducted to investigate the effects of dietary arginine levels on growth performance, body composition, serum biochemical indices and resistance ability against ammonia-nitrogen stress in juvenile yellow catfish(Pelteobagrus fulvidraco). Five isonitrogenous and isolipidic diets(42%protein and 9% lipid) were formulated to contain graded levels of arginine(2.44%, 2.64%, 2.81%, 3.01% and3.23% of diet), by supplementing L-Arginine HCI. Seven hundred juvenile yellow catfish with an initial average body weight of 1.13 ± 0.02 g were randomly divided into 5 groups with 4 replicates of 35 fish each and each group was fed one of the diets. After 56 d feeding, fish were exposed to 100 mg/L of ammonia-nitrogen for 72 h. The results showed that weight gain(WG) and specific growth rate(SGR) in2.64% and 2.81% groups were significantly higher than those in 3.23% group(P 0.05). After the fish were challenged to ammonia-nitrogen for 72 h, their cumulative mortality rate in 2.81% group was significantly lower than that in 2.44% group(P < 0.05). The results suggested that dietary arginine level at 2.81%could optimize anti-ammonia-nitrogen stress ability of juvenile yellow catfish and a level of 3.23%arginine seemed to depress the growth performance of fish and decreased their tolerance to the ammonia-nitrogen st

关键词： Arginine Yellow catfish(Pelteobagrus fulvidraco) Growth performance Body composition Serum biochemical indices Ammonia-nitrogen stress